• Datos Personales

    • Identidad

      Nombre en citaciones bibliográficas: Gomez MM
      Documento: Cédula de identidad - 33392042 ,Pasaporte - C724848
      Género: Femenino
      Fecha de nacimiento: 10/11/1983
      País de nacimiento: Uruguay
      Ciudad de nacimiento: Mercedes
      Nacionalidad: Uruguaya
      Estado civil: Divorciado
      Fecha de nacimiento de hijos:
      25/08/2014
    • Dirección personal

      Dirección: Juan Paullier 1369, 203 / 11200 / Montevideo / Montevideo / Uruguay
      Teléfono: 092628797
      Correo electrónico: marielamartinezgomez@gmail.com
  • Datos Generales

    • Institución principal

      Ministerio de Educación y Cultura/ MEC. Instituto de Investigaciones Biológicas «Clemente Estable» / División Biología Molecular y Genética - Departamento de Biología Molecular / Uruguay
    • Dirección institucional

      Institución: Ministerio de Educación y Cultura / MEC. Instituto de Investigaciones Biológicas «Clemente Estable» / Sector Gobierno/Público
      / División Biología Molecular y Genética - Departamento de Biología Molecular
      Dirección: Avenida Italia, 3318 / 11600 / Montevideo , Montevideo , Uruguay
      Teléfono: (+598) 2487-1616
      Correo electrónico/Sitio Web: mgomez@iibce.edu.uy http://www.iibce.edu.uy/
  • Formación

    • Formación académica

      • Concluida

        • MBA

          • Pos graduación Executiva en Medio Ambiente (2011 - 2012)
            Instituto Alberto Luiz Coimbra de Pós-Graduação e Pesquisa de Engenharia , Brasil
            Título de la disertación/tesis/defensa: Qualidade das águas e vírus entéricos como marcadores moleculares de contaminação por esgoto
            Tutor/es: Marcio de Souza Soares de Almeida
            Obtención del título: 2012
            Palabras Clave: gestión medio ambiente
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias de la Tierra y relacionadas con el Medio Ambiente / Ciencias de la Tierra y relacionadas con el Medio Ambiente / Gestión Ambiental

          Doctorado

          • Programa de pos Pos graduación en Biología Celular y Molecular (2010 - 2014)
            Instituto Oswaldo Cruz, Fundação Oswaldo Cruz , Brasil
            Título de la disertación/tesis/defensa: Diversidade genética dos Rotavírus da espécie A detectados antes e após a introdução da vacina monovalente no Brasil
            Tutor/es: JOSE PAULO GAGLIARDI LEITE
            Obtención del título: 2014
            Palabras Clave: Rotavírus CARACTERIZACIÓN MOLECULAR Epidemiologia molecular Biología Molecular evolución secuenciamiento filogenia
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica

          Maestría

          • Programa de pos Pos graduación en Biología Celular y Molecular (2007 - 2009)
            Instituto Oswaldo Cruz, Fundação Oswaldo Cruz , Brasil
            Título de la disertación/tesis/defensa: Análise filogenética dos genes que codificam as proteínas VP4, VP6, VP7 e NSP4 de amostras brasileiras de Rotavírus A, genótipo P[4],G2.
            Tutor/es: Jose Paulo Gagliardi Leite
            Obtención del título: 2009
            Palabras Clave: Rotavírus P[4]G2 VP4 VP6 VP7 e NSP4 Epidemiología molecular Evolución virus RNA Biología molecular
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
        • Grado

          • Licenciatura en Ciencias Biológicas (2002 - 2006)
            Universidad de la República - Facultad de Ciencias - UDeLaR , Uruguay
            Título de la disertación/tesis/defensa: Cepas del vírus de la Hepatitis C aisladas en Bolívia y Colombia revelan mutaciones específicas características de la Región Sudamericana
            Tutor/es: Juan Cristina
            Obtención del título: 2007
            Palabras Clave: Hepatitis C Evolución viral diversidad genética secuenciamiento PCR
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
    • Formación complementaria

      • Concluida

        • Posdoctorados

          • Beca de estadía postdoctoral en Brasil - Programa CAPES: Plano Nacional de Enfrentamiento al Aedes Aegypti y a la Microcefalia (2016 - 2018)
            Sector Extranjero/Internacional/Otros / Instituto Oswaldo Cruz, Fundação Oswaldo Cruz / Laboratório de Biologia Molecular de Flavivírus , Brasil
            Financiación:
            CAPES , Brasil
            Palabras Clave: zika epidemiología molecular biología molecular virus de la fiebre amarilla evolución de virus RNA caracterización molecular
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
          • Postdoctorado Nota 10 (PDR10) (2015 - 2016)
            Sector Extranjero/Internacional/Otros / Instituto Oswaldo Cruz, Fundação Oswaldo Cruz , Brasil
            Financiación:
            Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro , Brasil
            Palabras Clave: virus entéricos evolución de virus RNA epidemiologia molecular biología molecular
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virología
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
          • Postdoctorado Junior (PDJ) (2014 - 2015)
            Sector Extranjero/Internacional/Otros / Instituto Oswaldo Cruz, Fundação Oswaldo Cruz , Brasil
            Financiación:
            CNPq , Brasil
            Palabras Clave: rotavirus evolución de virus RNA epidemiología molecular biología molecular
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
        • Cursos de corta duración

          • Next generation sequencing technologies for viral metagenomic analyses (09/2016 - 09/2016)
            Sector Extranjero/Internacional/Otros / Sociedad Brasileira de Virologia , Brasil
            Palabras Clave: Next generation sequencing virus biología molecular
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / secuenciamiento de tercera generación
          • Gestão de projetos (01/2014 - 01/2014)
            Sector Extranjero/Internacional/Enseñanza superior / Fundação Getúlio Vargas - RJ , Brasil
            30 horas
          • Curso sobre NORMA ISO/IEC 17025:2005 en laboratorio de ensayo y calibración. (01/2013 - 01/2013)
            Sector Gobierno/Público / Laboratorio Tecnológico del Uruguay / Laboratorio Tecnológico del Uruguay , Uruguay
            20 horas
          • 17th International bioinformatics workshop on virus evolution and molecular epidemiology (01/2012 - 01/2012)
            Sector Extranjero/Internacional/Otros / KU Leuven , Bélgica
          • V Curso de biossegurança em laboratórios de pesquisa biomédica. Módulo Risco Biológico (01/2010 - 01/2010)
            Sector Extranjero/Internacional/Otros / Instituto Oswaldo Cruz , Brasil
            27 horas
          • V Curso de biossegurança em laboratórios de pesquisa biomédica. Módulo Experimentação animal. (01/2010 - 01/2010)
            Sector Extranjero/Internacional/Otros / Instituto Oswaldo Cruz , Brasil
            32 horas
          • V Curso de biossegurança em laboratórios de pesquisa biomédica. Módulo Risco Físico. (01/2010 - 01/2010)
            Sector Extranjero/Internacional/Otros / Instituto Oswaldo Cruz , Brasil
            18 horas
          • Replicative and Evolutionary Aspects of Hepatitis viruses (01/2007 - 01/2007)
            Sector Educación Superior/Público / Universidad de la República / Facultad de Ciencias - UDeLaR , Uruguay
          • Curso regional de capacitación sobre buenas prácticas de laboratorio aplicadas a laboratorio de biología molecular (01/2006 - 01/2006)
            Sector Extranjero/Internacional/Otros / Instituto Nacional de Salud , Perú
          • Genómica y Introducción a las herramientas de bioinformática (PEDECIBA) (01/2005 - 01/2005)
            Sector Educación Superior/Público / Universidad de la República / Facultad de Ciencias - UDeLaR , Uruguay
          • Phylogeny and Evolution o Viruses (ICGEB-PAHO-RELAB) (01/2005 - 01/2005)
            Sector Educación Superior/Público / Universidad de la República / Facultad de Ciencias - UDeLaR , Uruguay
          • Curso de técnicas moleculares y radioisotópicas aplicadas al diagnóstico de patologías humanas (01/2005 - 01/2005)
            Sector Educación Superior/Público / Universidad de la República / Facultad de Ciencias - UDeLaR , Uruguay
          • Biodiversidad y Conservación (01/2004 - 01/2004)
            Sector Educación Superior/Público / Universidad de la República / Facultad de Veterinaria - UDeLaR , Uruguay
          • Etología: el estudio del comportamiento animal. (01/2004 - 01/2004)
            Sector Educación Superior/Público / Universidad de la República / Facultad de Veterinaria - UDeLaR , Uruguay
        • Participación en eventos

          • II Jornadas de la Red Interinstitucional de Metagenómica Ambiental (RIMA) (2019)
            Tipo: Otro
            Palabras Clave: metagenomica medio ambiente
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología Celular, Microbiología /
          • Workshop Writing a Scientific Paper. (2018)
            Tipo: Otro
            Institución organizadora: Instituto Oswaldo Cruz, Fiocruz, Brasil
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Otros Tópicos Biológicos / Escrita de artículos científicos
          • QIAday – Sequenciamento de nova geração (NGS) (2017)
            Tipo: Seminario
            Institución organizadora: QIAGEN Biotecnologia, Brasil
          • Painel Febre Amarela: Monitoramento de epizootias no estado do Rio de Janeiro. (2017)
            Tipo: Seminario
            Institución organizadora: Instituto Oswaldo Cruz - Fiocruz, Brasil
          • III Simpósio Internacional de Imunobiológicos. Instituto de Tecnologia em Imunobiológicos (2016)
            Tipo: Simposio
            Institución organizadora: Bio-Manguinhos / Fiocruz, Brasil
          • XXVII Congresso Brasileiro de Virologia, XI Encontro de Virologia do Mercosul (2016)
            Tipo: Congreso
            Institución organizadora: Sociedade Brasileira de Virologia, Brasil
          • Workshop: Arboviroses emergentes: Chikungunya e Zika - III Simpósio Internacional de Imunobiológicos. Instituto de Tecnologia em Imunobiológicos (2016)
            Tipo: Otro
            Institución organizadora: Bio-Manguinhos / Fiocruz, Brasil
          • 12th International Rotavirus Symposium, Melbourne, Australia. (2016)
            Tipo: Simposio
            Institución organizadora: Sabin, Australia
          • II DSSA Debate-Praias limpas e emissão de emissários submarinos de esgotos, problema ou solução? (2015)
            Tipo: Seminario
            Institución organizadora: Departamento de saneamento e saúde ambiental, Escola Nacional de Saúde Pública Sergio Aroucena / Fiocruz, Brasil
          • II Seminário Anual Científico e Tecnológico em Imunobiológicos (2014)
            Tipo: Simposio
            Institución organizadora: Bio-Manguinhos / Fiocruz, Brasil
          • II Simpósio Hermann Schatzmayr de Virologia (2013)
            Tipo: Simposio
            Institución organizadora: Instituto Oswaldo Cruz - Fiocruz, Brasil
          • II Simposio Latinoamericano de Virología Ambiental (2013)
            Tipo: Simposio
            Institución organizadora: UdelaR, Uruguay
          • X Congreso Argentino de Virología, III Simposio de virología clínica, I Simposio de virología veterinaria (2011)
            Tipo: Congreso
            Institución organizadora: Asociación Argentina de Microbiología - AAM, Argentina
          • 4th European Rotavirus Biology Symposium (2011)
            Tipo: Simposio
            Institución organizadora: Sabin / GSK / Universidad de Roma, Italia
          • Harnessing Nature´s Powerful Sequencing Engine: Single-Molecular Real-time DNA Sequencing (2010)
            Tipo: Seminario
            Institución organizadora: Uniscience / Fiocruz, Brasil
          • I Latin American Symposium of Environmental Virology (2010)
            Tipo: Simposio
            Institución organizadora: Instituto Oswaldo Cruz - Fiocruz, Brasil
          • Técnicas inovadoras para concentração de amostras (2010)
            Tipo: Seminario
            Institución organizadora: GeneVac / Fiocruz, Brasil
          • Herpesvirus based vaccines against rotavirus infection – HEVAR Conference day (2009)
            Tipo: Seminario
            Institución organizadora: Instituto Oswaldo Cruz, Brasil
          • XX Encontro Nacional de Virologia. Brasilia, Brasil. (2009)
            Tipo: Encuentro
            Institución organizadora: Sociedade Brasileira de Virologia, Brasil
          • XIX National Meeting of Virology (2008)
            Tipo: Congreso
            Institución organizadora: Sociedade Brasileira de Virologia. SBV, Brasil
          • Avanços em Engenharia de Proteínas e Síntese de Peptídeos (2008)
            Tipo: Otro
            Institución organizadora: IOC / FIOCRUZ, Brasil
          • IV Escola de Modelagem Molecular em Sistemas Biológicos (2008)
            Tipo: Encuentro
            Institución organizadora: LNCC, Brasil
          • XIV Jornadas de Jóvenes Investigadores de la AUGM (2006)
            Tipo: Encuentro
            Institución organizadora: AUGM, Uruguay
          • I Seminario Biomédico del Instituto Pasteur de Montevideo (2006)
            Tipo: Seminario
            Institución organizadora: Instituto Pasteur, Uruguay
          • V Jornadas de Bioquímica y Biología Molecular (2006)
            Tipo: Encuentro
            Institución organizadora: SBBM, Uruguay
          • VIII Congreso Argentino de Virología (2005)
            Tipo: Congreso
            Institución organizadora: Sociedad Argentina de Virología, Argentina
      • En marcha

        • Posdoctorados

          • Postdoctorado IIBCE (2019)
            Sector Gobierno/Público / Ministerio de Educación y Cultura / MEC. Instituto de Investigaciones Biológicas «Clemente Estable» / ?División Biología Molecular y Genética - Departamento de Biología Molecular , Uruguay
            Financiación:
            Ministerio de Educación y Cultura / MEC. Instituto de Investigaciones Biológicas «Clemente Estable» , Uruguay
            Palabras Clave: virus Y de la papa epidemiología molecular caracterización molecular genoma caracterización biológica Uruguay
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Químicas / Ciencias Químicas /
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virología
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Genómica
  • Idiomas

    • Portugués
      Entiende muy bien / Habla muy bien / Lee muy bien / Escribe muy bien
    • Español
      Entiende muy bien / Habla muy bien / Lee muy bien / Escribe muy bien
    • Inglés
      Entiende muy bien / Habla bien / Lee muy bien / Escribe bien
  • Areas de actuación

    • Ciencias Naturales y Exactas

      Ciencias Biológicas /Bioquímica y Biología Molecular /Virología
    • Ciencias Médicas y de la Salud

      Ciencias de la Salud /Enfermedades Infecciosas /Virología
    • Ciencias Naturales y Exactas

      Ciencias Químicas /Ciencias Químicas
    • Ciencias Agrícolas

      Otras Ciencias Agrícolas /Otras Ciencias Agrícolas /Fitopatógenos
    • Ciencias Naturales y Exactas

      Ciencias Biológicas /Biología y Biología de la Evolución /Genómica
  • Actuación profesional

    • Sector Educación Superior/Público - Programa de Desarrollo de las Ciencias Básicas - Uruguay

      Área Química (PEDECIBA)

      • Vínculos con la Institución

        • Funcionario/Empleado (07/2019 - a la fecha)
          Investigador Grado 3 ,1 hora semanal
    • Sector Gobierno/Público - Ministerio de Educación y Cultura - Uruguay

      MEC. Instituto de Investigaciones Biológicas «Clemente Estable» / División Biología Molecular y Genética - Departamento de Biología Molecular

      • Vínculos con la Institución

        • Funcionario/Empleado (01/2019 - a la fecha)Trabajo relevante
          Posdoctorado ,40 horas semanales / Dedicación total
      • Actividades

        • Líneas de investigación

          • Caracterización biológica y molecular del virus Y de la papa en cultivares de papa (Solanum tuberosum) en Uruguay y diseño de herramientas de diagnóstico molecular para detección y caracterización del virus. (01/2019 - a la fecha )
            La papa (Solanum tuberosum), cultivada a nivel mundial, constituye el tercer cultivo en importancia luego del trigo y el arroz. Es susceptible a una serie de enfermedades que reducen el rendimiento y la calidad de los tubérculos. Se han identificado más de 40 virus que la afectan, siendo el de mayor importancia a nivel mundial el virus Y de la papa (Potato Virus Y- PVY). El PVY pertenece a la familia Potyviridae, género Potyvirus, es el agente causal de la enfermedad denominada mosaico severo o mosaico rugoso, responsable de importantes pérdidas en el cultivo. Más del 90% de las variedades cultivadas en Uruguay son susceptibles al PVY, entre otras virosis. Este virus se caracteriza por una gran variabilidad genética, últimamente se han encontrado genomas recombinantes de PVY que pueden tener efectos más negativos en los cultivares de papa que las "no recombinantes". Este proyecto tiene como objetivo, por un lado, caracterizar las variantes del PVY que circulan en nuestro país. El último relato científico de virus en papa en nuestro país data de 1983. A partir de sintomatología obtenida por inoculación en distintas solanáceas, se realizará su caracterización biológica, y con la secuenciación del genoma completo, su caracterización molecular y filogenia en relación a cepas ya caracterizadas a nivel mundial. Por otro lado, con base en estos datos se diseñarán metodologías moleculares de diagnóstico que permitan simultáneamente una rápida detección y caracterización molecular de las cepas de PVY en Uruguay, y en otras regiones. Estas herramientas podrán ser transferidas a los servicios oficiales de protección vegetal así como a empresas nacionales dedicadas al diagnóstico fitopatológico.
            Mixta
            40 horas semanales
            División Biología Molecular y Genética - Departamento de Biología Molecular , Integrante del equipo
            Equipo: Mariela MARTINEZ GOMEZ , Inés PONCE DE LEÓN TADEO , Peyrou M
            Palabras clave: genoma PVY secuenciación Uruguay detección y diagnóstico
            Areas de conocimiento:
            Ciencias Agrícolas / Otras Ciencias Agrícolas / Otras Ciencias Agrícolas / Fitopatógenos
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
        • Extensión

          • Recepción de visitas escolares en el IIBCE, actividad didáctica en el laboratorio. (05/2019 - a la fecha )
            2 horas
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular /
            Ciencias Naturales y Exactas / Ciencias Biológicas / Ciencias Biológicas /
          • Participación en el marco del aniversario del Prof. Clemente Estable y de la Semana de la Ciencia y la Tecnología - Actividades con niños de escuela / Enseñando sobre el ADN (01/2019 - a la fecha )
            4 horas
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular /
    • Sector Extranjero/Internacional/Otros - Brasil

      Instituto Oswaldo Cruz / Ministério de Saúde Pública - MSP

      • Vínculos con la Institución

        • Otro (02/2014 - 12/2018)Trabajo relevante
          Becaria de Post-doctorado ,40 horas semanales / Dedicación total
        • Becario (02/2010 - 01/2014)
          Estudiante de Doctorado ,40 horas semanales / Dedicación total
        • Becario (09/2007 - 10/2009)
          Estudiante de Maestría ,40 horas semanales / Dedicación total
      • Actividades

        • Líneas de investigación

          • Programa CAPES: Plano Nacional de Enfrentamiento al Aedes Aegypti y a la Microcefalia. (01/2016 - 12/2018 )
            Desarrollar investigación científica en el área del postdoctorado propuesta (caracterización molecular, detección y diagnóstico, epidemiología molecular del virus Zika) y otras áreas relacionadas al laboratorio. Ministrar cursos y conferencias, orientar alumnos de iniciación científica, publicar artículos científicos, participar de seminarios científicos, ayudar con el entrenamiento de los recursos humanos que ingresen al laboratorio.
            40 horas semanales
            Laboratorio de Biologia Molecular de Flavivirus, Instituto Oswaldo Cruz - IOC , Integrante del equipo
            Equipo: BONALDO , LOURENCO-DE-OLIVEIRA
            Palabras clave: Zika Diversidad genetica Filogenia fiebre amarilla genoma filogeografía epidemiologia molecular
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
            Ciencias Naturales y Exactas / Ciencias de la Computación e Información / Ciencias de la Información y Bioinformática /
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
          • Caracterización molecular, detección, diagnóstico y epidemiología molecular de rotavirus de la especie A (09/2007 - 09/2016 )
            Desarrollar investigación científica en el área del postdoctorado propuesta (caracterización molecular, detección, diagnóstico y epidemiología molecular de rotavirus) y otras áreas relacionadas al laboratorio. Ministrar cursos y conferencias, orientar alumnos de iniciación científica, publicar artículos científicos, participar de seminarios científicos, ayudar con el entrenamiento de los recursos humanos que ingresen al laboratorio.
            40 horas semanales
            Laboratorio de Virologia Comparada e Ambiental, Laboratorio de Referencia R, Instituto Oswaldo Cruz - IOC , Integrante del equipo
            Equipo: GAGLIARDI LEITE , COLINA , Luis Fernando LÓPEZ TORT , Volotao EM , Juan CRISTINA GHERALDI
            Palabras clave: Rotavírus Diversidad genetica Filogenia secuenciamiento genoma completo diagnóstico
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
        • Docencia

          • Pos graduación Stricto sensu en Biología Celular y Molecular / Pos graduación Stricto sensu en Biología Parasitaria (01/2008 - 12/2017 )
            Doctorado
            Invitado
            Asignaturas:
            Dinâmica das Gastrenterites Agudas de Etiologia Viral, 2 horas, Teórico-Práctico
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Ciencias Biológicas / Virología
          • Curso Técnico em Biotecnologia / IOC - Fiocruz (01/2016 - 12/2016 )
            Grado
            Invitado
            Asignaturas:
            Virología, 2 horas, Teórico
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Ciencias Biológicas / Virología
          • Curso de verao - Metodologias aplicadas ao estudo das infecções virais. (01/2011 - 12/2011 )
            Grado
            Invitado
            Asignaturas:
            Metodologias aplicadas ao estudo das infeccoes virais, 2 horas, Teórico-Práctico
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Ciencias Biológicas / Virología
  • Carga horaria

    • Carga horaria de docencia: 5 horas
    • Carga horaria de investigación: 40 horas
    • Carga horaria de formación RRHH: 10 horas
    • Carga horaria de extensión: 5 horas
    • Carga horaria de gestión: Sin horas
  • Producción científica/tecnológica

    • Es fundamental conocer las cepas de virus del PVY circulantes en las diferentes zonas paperas de nuestro país para minimizar la incidencia del mismo de forma eficaz y disminuir su impacto económico. Con este objetivo estamos realizando estudios de caracterización biológica, molecular y filogenética de las cepas del PVY circulantes en los cultivares de papa en el Uruguay. Con base en los dados de secuencias obtenidos iremos diseñar una herramienta de diagnóstico molecular que permita de forma conjunta la detección y caracterización del virus. La aplicación de esta herramienta permitirá la rápida caracterización de las cepas de PVY circulantes. Pretendemos contribuir para disminuir de forma significativa los efectos perjudiciales del PVY en el rendimiento y la calidad de los tubérculos nacionales, así como en reducir la importación de tubérculos y papa semilla, y por consiguiente, limitar el posible ingreso de nuevas enfermedades a nuestro país.

  • Producción bibliográfica

    • Artículos publicados

      • Arbitrados

        • The evolving epidemiology of rotavirus A infection in Brazil a decade after the introduction of universal vaccination with Rotarix® (Completo, 2019)
          Carvalho-Costa FA , de Assis RMS , Fialho AM , Araújo IT , Silva MF , Gomez MM , Andrade JS , Rose TL , Fumian TM , Volotão EM , Miagostovich MP , Leite JPG
          BMC Pediatrics, v.: 19 42 , p.:1 - 10, 2019
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Epidemiologia de rotavirus
          Medio de divulgación: Internet
          ISSN: 14712431
          DOI: 10.1186/s12887-019-1415-9
          https://bmcpediatr.biomedcentral.com/articles/10.1186/s12887-019-1415-9
          Background: Brazil introduced the monovalent rotavirus vaccine (Rotarix®) in 2006. This study aimed to assess the epidemiology and genotype distribution of species-A rotavirus (RVA) in Brazil, comparing the pre- and postvaccination periods. Methods: Laboratory-based RVA surveillance included 866 municipalities in 22 Brazilian states, over a 21-year period. A total of 16,185 children with diarrheal diseases (DD) aged up to 12 years between 1996 and 2005 (pre-vaccination period, n = 7030) and from 2006 to 2017 (post-vaccination period, n = 9155) were enrolled. RVA was detected using ELISA immune assay and/or polyacrylamide gel electrophoresis and genotyped using nested PCR and/or nucleotide sequencing. RVA-positivity and genotypes detection rates were compared in distinct periods and age groups and Rotarix vaccination status. Results: RVA-positivity in pre- and post-vaccination periods was, respectively: 4?11 months bracket, 33.3% (668/2006) and 16.3% (415/2547) (p < 0.001); 12?24 months, 28.2% (607/2154) and 22.2% (680/3068) (p< 0.001); 25?48 months, 17.4% (215/1235) and 29.4% (505/1720) (p < 0.001). Genotypes distribution in the pre- and post-vaccination periods was, respectively: G1P [8]/G1P[Not Typed], 417/855 (48.8%) and 118/1835 (6.4%) (p < 0.001); G2P [4]/G2P[NT], 47/855 (5.5%) and 838/1835 (45.7%) (p < 0.001); G3P [8]/G3P[NT], 55/855 (6.4%) and 253/1835 (13.8%) (p < 0.001); G9P [8]/G9P[NT], 238/855 (27.8%) and 152/1835 (8.3%) (p < 0.001); G12P [8]/G129P[NT], 0/871 (0%) and 249/1835(13.6%) (p < 0.001). Concerning infants aged 4?11 months, RVA frequency in fully vaccinated and non-vaccinated individuals was 11.9% (125/ 1052) and 24.5% (58/237) (p < 0.001), respectively. In children aged 12?24 months, RVA detection rate was 18.1% (253/ 1395) and 29.6% (77/260) (p < 0.001), for the vaccinated and non-vaccinated individuals, respectively (p < 0.001). Conclusions: RVA infection was significantly less frequent in children aged ?2 years with DD after implementing vaccination, mainly among vaccinated children. It was also observed a decrease of P [8] circulation and emergence of G2P[4] in 2005, and afterwards in the post-vaccine era, with spreading of G12P[8] in 2014?2015 and of G3P[8] in 2017. Continuous RVA surveillance must be carried out in this scenario.

        • Haemagogus leucocelaenus and Haemagogus janthinomys are the primary vectors in the major yellow fever outbreak in Brazil, 2016-2018 (Completo, 2019)
          Abreu FS , Ribeiro I , Brito AF , Santos A , Miranda R , Gomez MM , Bonaldo M , Lourenco-de-Oliveira R
          Emerging Microbes & Infections, v.: 8 1 , p.:218 - 231, 2019
          Palabras clave: Yellow fever; Atlantic forest; Haemagogus; Aedes; Sabethes
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
          Medio de divulgación: Internet
          ISSN: 22221751
          DOI: 10.1080/22221751.2019.1568180
          https://www.tandfonline.com/doi/abs/10.1080/22221751.2019.1568180
          The yellow fever virus (YFV) caused a severe outbreak in Brazil in 2016?2018 that rapidly spread across the Atlantic Forest in its most populated region without viral circulation for almost 80 years. A comprehensive entomological survey combining analysis of distribution, abundance and YFV natural infection in mosquitoes captured before and during the outbreak was conducted in 44 municipalities of five Brazilian states. In total, 17,662 mosquitoes of 89 species were collected. Before evidence of virus circulation, mosquitoes were tested negative but traditional vectors were alarmingly detected in 82% of municipalities, revealing high receptivity to sylvatic transmission. During the outbreak, five species were found positive in 42% of municipalities. Haemagogus janthinomys and Hg. leucocelaenus are considered the primary vectors due to their large distribution combined with high abundance and natural infection rates, concurring together for the rapid spread and severity of this outbreak. Aedes taeniorhynchus was found infected for the first time, but like Sabethes chloropterus and Aedes scapularis, it appears to have a potential local or secondary role because of their low abundance, distribution and infection rates. There was no evidence of YFV transmission by Aedes albopictus and Aedes aegypti, although the former was the most widespread species across affected municipalities, presenting an important overlap between the niches of the sylvatic vectors and the anthropic ones. The definition of receptive areas, expansion of vaccination in the most affected age group and exposed populations and the adoption of universal vaccination to the entire Brazilian population need to be urgently implemented.

        • Distinct YFV Lineages Co-circulated in the Central-Western and Southeastern Brazilian Regions From 2015 to 2018 (Completo, 2019)
          Delatorre , Santos de Abreu , Pereira Ribeiro , Gomez MM , Araújo Cunha dos Santos , Ferreira-de-Brito , Santos Neves , Bonelly , Moraes de Miranda , Dias Furtado , Menezes Souza Raphael , Fernandes da Silva , Gonçalves de Castro , Garkauskas Ramos , Martins Romano , Kallás , Paulo Vicente , Bello , Lourenço-de-Oliveira , Cristina Bonaldo
          Frontiers in Microbiology, v.: 10 p.:1 - 12, 2019
          Palabras clave: yellow fever Brazilian outbreak lineages amino acid changes phylogeography
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
          Medio de divulgación: Internet
          ISSN: 1664302X
          DOI: 10.3389/fmicb.2019.01079
          https://www.frontiersin.org/article/10.3389/fmicb.2019.01079
          The current outbreak of yellow fever virus (YFV) that is afflicting Brazil since the end of 2016 probably originated from a re-introduction of YFV from endemic areas into the non-endemic Southeastern Brazil. However, the lack of genomic sequences from endemic regions hinders the tracking of YFV?s dissemination routes. We assessed the origin and spread of the ongoing YFV Brazilian outbreak analyzing a new set of YFV strains infecting humans, non-human primates (NHPs) and mosquitoes sampled across five Brazilian states from endemic and non-endemic regions between 2015 and 2018. We found two YFV sub-clade 1E lineages circulating in NHP from Goiás state (GO), resulting from independent viral introductions into the Araguaia tributary river basin: while the strain from 2017 clustered intermingled with Venezuelan YFV strains from 2000, the YFV strain sampled in 2015 clustered with sequences of the current YFV outbreak in the Brazilian Southeastern region (named YFV2015?2018 lineage), displaying the same molecular signature associated to the current YFV outbreak. After its introduction in GO at around mid-2014, the YFV2015?2018 lineage followed two paths of dissemination outside GO, originating two major YFV sub-lineages: (1) the YFVMG/ES/RJ sub-lineage spread sequentially from the eastern area of Minas Gerais state to Espírito Santo and then to Rio de Janeiro states, following the Southeast Atlantic basin; (2) the YFVMG/SP sub-lineage spread from the southwestern area of Minas Gerais to the metropolitan region of São Paulo state, following the Paraná basin. These results indicate the ongoing YFV outbreak in Southeastern Brazil originated from a dissemination event from GO almost 2 years before its recognition at the end of 2016. From GO this lineage was introduced in Minas Gerais state at least two times, originating two sub-lineages that followed different routes toward densely populated areas. The spread of YFV outside endemic regions for at least 4 years stresses the imperative importance of the continuous monitoring of YFV to aid decision-making for effective control policies aiming the increase of vaccination coverage to avoid the YFV transmission in densely populated urban centers.

        • G26P[19] rotavirus A strain causing acute gastroenteritis in the American continent. (Completo, 2018)Trabajo relevante
          Gomez MM , da Silva MFM , Fialho AM , Mazzoco CS , Rocha MS , Leite JPG
          Memórias do Instituto Oswaldo Cruz, v.: 113 12 , p.:1 - 4, 2018
          Palabras clave: rotavirus A G26P19 American continent genome constellation gastroenterite reassortment
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus
          Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
          Medio de divulgación: Internet
          ISSN: 00740276
          DOI: 10.1590/0074-02760180344
          http://memorias.ioc.fiocruz.br/
          In Brazil, the rotavirus A genotype G26 was first identified in suckling piglets, while the P[19] genotype has not been identified in any animal species so far. This report details the genetic characterisation of a G26P[19] RVA strain detected from an eight year-old child, vaccinated with Rotarix®, hospitalised with acute diarrhoeal disease in Rio de Janeiro in 2015. Most likely, the genome constellation (I5-R1-C1-M1-A8-N1-T1-E1-H1) observed in the G26P[19] Brazilian strain was a result of interspecies transmission events between humans and pigs. In addition, a rearrangement in the NSP5 gene was observed downstream of the 3? non-coding region.

        • Genomic and structural features of the yellow fever virus from the 2016-2017 Brazilian outbreak (Completo, 2017)Trabajo relevante
          Gomez MM , Abreu FVS , Santos AACD , Mello IS , Santos MP , Ribeiro IP , Ferreira-de-Brito A , Miranda RM , Castro MG , Ribeiro MS , Laterrière Junior RDC , Aguiar SF , Meira GLS , Antunes D , Torres PHM , Mir D , Vicente ACP , Guimarães ACR , Caffarena ER , Bello G , Lourenço-de-Oliveira R , Bonaldo MC
          Journal of General Virology, v.: 99 4 , p.:536 - 548, 2017
          Palabras clave: Yellow fever virus 2016-2017 Brazilian outbreak amino acid changes genetic diversity evolution
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virología
          Medio de divulgación: Internet
          ISSN: 00221317
          DOI: 10.1099/jgv.0.001033
          Southeastern Brazil has been suffering a rapid expansion of a severe sylvatic yellow fever virus (YFV) outbreak since late 2016, which has reached one of the most populated zones in Brazil and South America, heretofore a yellow fever-free zone for more than 70?years. In the current study, we describe the complete genome of 12 YFV samples from mosquitoes, humans and non-human primates from the Brazilian 2017 epidemic. All of the YFV sequences belong to the modern lineage (sub-lineage 1E) of South American genotype I, having been circulating for several months prior to the December 2016 detection. Our data confirm that viral strains associated with the most severe YF epidemic in South America in the last 70 years display unique amino acid substitutions that are mainly located in highly conserved positions in non-structural proteins. Our data also corroborate that YFV has spread southward into Rio de Janeiro state following two main sylvatic dispersion routes that converged at the border of the great metropolitan area comprising nearly 12?million unvaccinated inhabitants. Our original results can help public health authorities to guide the surveillance, prophylaxis and control measures required to face such a severe epidemiological problem. Finally, it will also inspire other workers to further investigate the epidemiological and biological significance of the amino acid polymorphisms detected in the Brazilian 2017 YFV strains.

        • Genome analysis of yellow fever virus of the ongoing outbreak in Brazil reveals polymorphisms. (Completo, 2017)Trabajo relevante
          BONALDO MC , Gomez MM , DOS SANTOS AA , Abreu FVS , FERREIRA-DE-BRITO A , MIRANDA RM , CASTRO MG , LOURENCO-DE-OLIVEIRA R
          Memorias do Instituto Oswaldo Cruz (E), v.: 112 6 , p.:447 - 451, 2017
          Palabras clave: Yellow fever NS3 NS5 polymorphisms Brazil 2017
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Virus de la Fiebre Amarilla
          ISSN: 16788060
          DOI: 10.1590/0074-02760170134
          The current yellow fever outbreak in Brazil is the most severe one in the country in recent times. It has rapidly spread to areas where YF virus (YFV) activity has not been observed for more than 70 years and vaccine coverage is almost null. Here, we sequenced the whole YFV genome of two naturally infected howler-monkeys (Alouatta clamitans) obtained from the Municipality of Domingos Martins, state of Espírito Santo, Brazil. These two ongoing-outbreak genome sequences are identical. They clustered in the 1E sub-clade (South America genotype I) along with the Brazilian and Venezuelan strains recently characterised from infections in humans and non-human primates that have been described in the last 20 years. However, we detected eight unique amino acid changes in the viral proteins, including the structural capsid protein (one change), and the components of the viral replicase complex, the NS3 (two changes) and NS5 (five changes) proteins, that could impact the capacity of viral infection in vertebrate and/or invertebrate hosts and spreading of the ongoing outbreak.

        • G1P[8] species A rotavirus over 27 years-pre- and post-vaccination eras--in Brazil: full genomic constellation analysis and no evidence for selection pressure by Rotarix® vaccine. (Completo, 2015)
          DA SILVA MF , ROSE TL , Gomez MM , CARVALHO-COSTA FA , FIALHO AM , ASSIS RM , ANDRADE J DA S , VOLOTãO EM , LEITE JPG
          Infection, Genetics and Evolution, v.: 30 p.:206 - 218, 2015
          Palabras clave: Acute gastroenteritis G1P[8] genomic constellation Reassortment Rotarix vaccine Species A rotavirus
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus de la especie A
          Medio de divulgación: Internet
          ISSN: 15671348
          DOI: 10.1016/j.meegid.2014.12.030
          Epidemiological data on species A rotavirus (RVA) infections have demonstrated the genetic diversity of strains circulating worldwide. Many G and P genotype combinations have been described over the years, varying regionally and temporally, especially in developing countries. However, the most common G and P genotype combinations identified in RVA human strains worldwide are G1P[8], G2P[4], G3P[8], G4P[8] and G9P[8]. RVA genotype G1P[8] strains are responsible for more than 50% of child infections worldwide and component of the two vaccines (Rotarix® [RV1] and RotaTeq® [RV5]) licensed globally. For a better understanding of the evolutionary mechanisms of this genotype in Brazil, phylogenetic analyses based on the 11 RVA genome segments (genomic constellation) from 90 G1P[8] RVA strains collected in two eras - (i) pre-vaccination with RV1 (1996-February 2006); (ii) post-vaccination (March 2006-2013) - in different Brazilian states were performed. The results showed the Wa-like genomic constellation of the Brazilian G1P[8] strains with a I1-R1-C1-M1-A1-N1-T1-E1-H1 specificity, except for two strains (rj14055-07 and ba19030-10) that belong to a I1-R1-C1-M1-A1-N1-T3-E1-H1 genomic constellation, evidencing the occurrence of reassortment (Wa-like×AU-1-like) of the NSP3 gene. Reassortment events were also demonstrated between Brazilian G1P[8] strains and the RV1 vaccine strain in some genes in vaccinated and unvaccinated children. VP7 and VP8* antigenic site analysis showed that the amino acid substitutions observed in samples collected after the introduction of RV1 in Brazil were already detected in samples collected in the 1980s and 1990s, suggesting that mass Brazilian RV1 vaccination had no impact on the diversity observed inside antigenic sites for these two proteins.

        • Environmental Assessment of Classical Human Astrovirus in Uruguay (Completo, 2015)
          LIZASOAIN A , TORT LF , GARCIA M , Gomez MM , CRISTINA J , LEITE JPG , MIAGOSTOVICH MP , VICTORIA M , COLINA R
          Food and Environmental Virology, v.: 7 2 , p.:142 - 148, 2015
          Palabras clave: Uruguay Astrovirus sewage samples
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Virologia Ambiental
          ISSN: 18670334
          DOI: 10.1007/s12560-015-9186-4
          The aim of this study was to determine the molecular epidemiology of classical human astrovirus (HAstV) strains in sewage samples from four Uruguayan cities: Bella Unión, Salto, Paysandú, and Fray Bentos, located in the Northwestern region of the country. Overall, 96 sewage samples were collected biweekly between March 2011 and February 2012 and were subject to ultracentrifugation methodology in order to concentrate the viruses. RT-PCR directed to the ORF2 genome region was performed followed by sequencing and phylogenetic analysis. Forty-three (45 %) out of 96 analyzed samples were positive for HAstV (Mamastrovirus 1) and 31 of them were successfully sequenced being 21 (49 %) of them classified as HAstV-1 genotype (1a lineage) and 10 (23 %) as HAstV-2 genotype (eight strains belonging to the 2d lineage and two strains to the 2c lineage). The 1a lineage circulated throughout the year, while the 2d lineage only in the coldest months (June to October). Strikingly, the 2c lineage was detected only in Salto city during March 2011. In this city it was observed the highest frequency of HAstV and the greatest genetic diversity, probably due to its role as high touristic spot with an important influx of visitants from others regions of Uruguay and also from other countries. This study constitutes the first report in Uruguay that describes the phylogenetic diversity and genotype distribution of HAstV strains circulating in the Northwestern region evidencing a high frequency and also the presence of several different lineages.

        • Molecular epidemiology of group A rotavirus among children admitted to hospital in Salto, Uruguay, 2011-2012: first detection of the emerging genotype G12. (Completo, 2015)
          TORT LF , VICTORIA M , LIZASOAIN A , CASTELLS M , MAYA L , Gomez MM , ARRESEIGOR E , LóPEZ P , CRISTINA J , LEITE JPG , COLINA R
          Journal of Medical Virology, v.: 87 5 , p.:754 - 763, 2015
          Palabras clave: Rotavirus A Uruguay G12
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus de la especie A
          Medio de divulgación: Internet
          ISSN: 01466615
          DOI: 10.1002/jmv.24123
          Group A rotavirus (RVA) is the most important etiologic agent of infant acute gastroenteritis (AGE) worldwide. Detection and molecular characterization of RVA in Salto department, Northwestern region of Uruguay, was conducted on 175 clinical samples, being 153 stool and 22 vomit samples, collected from hospitalized children with AGE, between 0-15 years old, from two hospitals of Salto city during 2011 and 2012. RVA was detected and genotyped by seminested multiplex RT-PCR in order to determine G- and P-genotypes. Positive samples were sequenced and phylogenetic analyses were carried out in order to determine lineages and sub-lineages. RVA were detected in 64 (37%) of the samples and the G and P genotypes observed were: 6% G1P[8], 23% G2P[4]/G2P[X]/GXP[4], 23% G3P[8]/G3P[X], 14% G12P[8]/G12P[X], 16% GXP[8], 1,5% G12P[9], 3% G2P[4]/[8], and 16% non-typeable. VP7 and VP4 genotypes related to DS-1 like gene constellation were prevalent during 2011 and those VP7 and VP4 genotypes related to Wa-like constellation were prevalent during 2012 (mainly represented by G3P[8]). Interestingly, RVA was detected in vomit samples in a high prevalence (41%). RVA was observed mainly in the age group between 1 and 5 years old (75% of the cases), and seasonality with a high detection rate in winter season was observed for the two consecutive years of surveillance. To our knowledge, this study represents the first detection and molecular characterization of RVA in Salto department, Northwestern region of Uruguay; and the first identification of the emerging genotype G12 in the country.

        • Detection of Common, Emerging and Uncommon VP4, and VP7 Human Group A Rotavirus Genotypes from Urban Sewage Samples in Uruguay. (Completo, 2015)
          TORT LF , LIZASOAIN A , GARCIA M , BEROIS M , CRISTINA J , LEITE JPG , Gomez MM , MIAGOSTOVICH MP , COLINA R
          Food and Environmental Virology, v.: 7 4 , p.:342 - 353, 2015
          Palabras clave: Rotavirus A sewage Uruguay
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Virologia Ambiental
          ISSN: 18670334
          DOI: 10.1007/s12560-015-9213-5
          Environmental approach has proven to be a useful tool for epidemiological studies demonstrating through environmental studies the diversity of viruses circulating in a given population. The aim of this study was to perform a phylogenetic characterization of the group A rotavirus (RVA) glycoprotein (G)- and protease-sensitive (P)-genotypes obtained from sewage samples (n = 116) collected in six cities of Uruguay during March 2011 to April 2013. A worldwide standardized semi-nested multiplex RT-PCR (SNM RT-PCR) protocol directed against VP4 and VP7 genes were conducted for RVA detection and consensual DNA fragments were submitted to nucleotide sequencing. P and/or G genotype was successfully determined by phylogenetic analysis in 61% (n = 37) of the positive samples obtained by SNM RT-PCR (n = 61). The RVA genotypes were as follow: G1 (n = 2), G2 (n = 14), G3 (n = 5), G12 (n = 2), P[4] (n = 4), P[8] (n = 16), and P[3] (n = 2). Interestingly, through phylogenetic analysis, emerging, and uncommon human genotypes could be detected. Results obtained from the comparison of RVA genotypes detected in the current study and Uruguayan RVA strains previously described for contemporary clinical pediatric cases showed that monitoring sewage may be a good screening option for a rapid and economical overview of the circulating genotypes in the surrounding human population and a useful approximation to study RVA epidemiology in a future vaccine monitoring program. The present study represents the first report in Uruguay that describes the phylogenetic diversity of RVA from urban sewage samples.

        • Group a rotavirus and norovirus genotypes circulating in the northeastern Brazil in the post-monovalent vaccination era. (Completo, 2015)
          Sá AC , Gomez MM , LIMA IF , QUETZ JS , HAVT A , ORIá RB , LIMA AA , LEITE JPG
          Journal of Medical Virology, v.: 87 9 , p.:1480 - 1490, 2015
          Palabras clave: Rotavirus A Norovirus Brazil
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Gastroenteritis Virales
          ISSN: 01466615
          DOI: 10.1002/jmv.24144
          Group A rotaviruses (RVA) and noroviruses (NoV) are the leading cause of acute gastroenteritis (AGE) worldwide. Childhood diarrhea deaths and hospital admissions have declined since the introduction of the monovalent (G1P[8]) vaccine (Rotarix(®) [RV1]) in the National Immunization Program in Brazil in 2006. This study aims to investigate the epidemiological profile of NoV and RVA infections from children with AGE in the Northeastern region of Brazil in the post vaccine season. Two-hundred fecal samples collected from children up to 10 years old in Fortaleza, Ceará between 2008-2009 were screened for the presence of RVA and NoV. Positive samples were genotyped and sequenced. The RVA screening revealed 12% prevalence and all RVA strains belonged to G2P[4] genotype. Phylogenetic analysis based on the 11 RVA genome segments sequenced from eight samples revealed a DS-1-like genotype constellation: I2-R2-C2-M2-A2-N2-T2-E2-H2. For NoV screening, the prevalence observed was 17% and the following genotypes were detected: GII.4 (59%), GII.12 (17%), GII.6 (9%), GII.3 (6%), and GII.? (9%). At least four different NoVs genotypes and two RVA G2P[4] variants were identified circulating in the Northeastern region of Brazil. RVA phylogenetic analysis suggests that the RVA G2P[4] strains might have originated from intragenogroup reassortment events. Whether the genetic modifications observed in these contemporary G2P[4] RVA strains may impact the long-term effectiveness of the current vaccination programs remains to be explored. These data reinforce the importance of surveillance for monitoring the emergence of new strains of RVA and NoV and their impact on cases of acute gastroenteritis.

        • Environmental assessment reveals the presence of MLB-1 human Astrovirus in Uruguay. (Completo, 2015)
          LIZASOAIN A , TORT LF , GARCIA M , Gomez MM , LEITE JPG , MIAGOSTOVICH MP , CRISTINA J , COLINA R , VICTORIA M
          Journal of Applied Microbiology, v.: 119 3 3, p.:859 - 867, 2015
          Palabras clave: Uruguay Astrovirus MLB-1
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Virologia Ambiental
          Medio de divulgación: Internet
          ISSN: 13645072
          DOI: 10.1111/jam.12856
          AIMS: The aim of this study was to investigate the presence of the recently identified human astrovirus (HAstV) and to increase the knowledge of the molecular epidemiology of classical HAstV detected in Uruguay. METHODS AND RESULTS: Recently identified and classical HAstV genotypes were investigated by RT-PCR targeting the ORF1b and ORF2 genome regions in 20 samples obtained between September 2011 and April 2013 in two cities of the eastern region of Uruguay. Four of 20 samples (20%) were identified as MLB-1 genotype and it was found a new MLB-1 classification through the segregation of the worldwide reported MLB-1 strains in two genetic lineages proposed and named: MLB-1a and MLB-1b. Fourteen (70%) samples were positive for classical HAstV and 12 of them were successfully sequenced and genotyped as: HAstV-1 (n = 10), HAstV-2 and HAstV-5 (one sample each). CONCLUSION: These results constitute the first report in the Latin American region concerning the molecular detection and characterization of MLB-1 HAstV strains in environmental samples. SIGNIFICANCE AND IMPACT OF THE STUDY: This study highlights the benefits of an environmental surveillance to study emerging enteric viruses circulating in human societies.

        • Sewage surveillance reveals the presence of canine GVII norovirus and canine astrovirus in Uruguay. (Completo, 2015)
          LIZASOAIN A , TORT FL , GARCIA M , Gomez MM , LEITE JPG , MIAGOSTOVICH MP , CRISTINA, J. , BEROIS M , COLINA, R. , VICTORIA M
          Archives of Virology, v.: 160 11 , p.:2839 - 2843, 2015
          Palabras clave: Norovirus Astrovirus
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Virologia Ambiental
          ISSN: 03048608
          DOI: 10.1007/s00705-015-2571-3
          Canine norovirus (NoV) and astrovirus (AstV) were studied in 20 domestic sewage samples collected in two cities in Uruguay. Four samples were characterized as canine AstV after phylogenetic analysis clustering with strains detected in Italy and Brazil in 2008 and 2012, respectively. One sample was characterized as canine NoV and clustered with a strain detected in Hong Kong and recently classified as GVII. This study shows the occurrence of a canine NoV GVII strain for the first time in the American continent and also warns about possible zoonotic infection, since canine strains were detected in domestic sewage.

        • Prevalence and genomic characterization of G2P[4] group A rotavirus strains during monovalent vaccine introduction in Brazil. (Completo, 2014)Trabajo relevante
          Gomez MM , Carvalho-Costa FA , Volotao E de M , Rose TL , da Silva MFM , Fialho AM , Assis RMS , Andrade JSR , Sá ACC , Zeller M , Heylen E , Matthijnssens J , Leite JPG
          Infection, Genetics and Evolution, v.: 28 p.:486 - 494, 2014
          Palabras clave: Rotavírus G2P[4] genetic characterization
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus de la especie A
          Medio de divulgación: Internet
          ISSN: 15671348
          DOI: 10.1016/j.meegid.2014.09.012
          This study aims to: estimate the prevalence of G2P[4] rotaviruses in Brazil between 2001-2011 from patients with acute gastroenteritis; perform phylogenetic analyses of G2P[4] Brazilian strains (from vaccinated and non-vaccinated children) based on VP7 and VP8(?) encoding genes and analyze the antigenic regions of these proteins comparing with RV1; and assess the full genetic background of eleven selected Brazilian strains. The G2P[4] detection rate among RVA positive samples was 0/157 in 2001, 3/226 (1.3%) in 2002, 0/514 in 2003, 0/651 in 2004, 31/344 (9%)/2005, 112/227 (49%)/2006, 139/211 (66%)/2007, 240/284 (85%)/2008, 66/176 (37.5%)/2009, 367/422 (87%)/2010 and 75/149 (50%)/2011. For the VP7 and VP8(?) encoding genes, 52 sequences were analyzed and shared up to 99% nucleotide identity with other contemporary G2P[4] strains detected worldwide, grouping into different clusters. Most differences inside antigenic epitopes of VP7 and VP8(?) have been maintained in the G2P[4] Brazilian strains along the years, and all were present before RV1 introduction. Eleven G2P[4] strains (4-vaccinated/7-non-vaccinated) were completely characterized and possessed the typical DS-1-like genotype constellation (G2-P[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) sharing up to 99% of nucleotide identity with contemporary worldwide strains. Reassortments between Brazilian G2P[4] human strains were observed. In conclusion, the data obtained in the current study suggests that implementation of RV1 vaccination might not influence the genetic diversity observed in G2P[4] analyzed strains. Several factors might have contributed to the increased prevalence of this genotype in Brazil since 2005: the introduction of RV1 into the Brazilian National Immunization Program has resulted in a decrease in the relative prevalence of predominant Wa-like RVA strains facilitating the increase of the heterotypic (DS-1-like) RVA strain G2P[4] in the Brazilian population; the genetic diversity found in different geographical regions throughout the years before, and after the introduction of RV1; the long period of low or no circulation of this genotype in Brazil previous to RV1 introduction could have created favorable conditions for the accumulation of immunological susceptible individuals.

        • Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil. (Completo, 2014)
          Gomez MM , RESQUE HR , VOLOTãO EM , ROSE TL , DA SILVA MF , HEYLEN E , ZELLER M , MATTHIJNSSENS J , LEITE JPG
          Infection, Genetics and Evolution, v.: 28 p.:385 - 388, 2014
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus de la especie A
          Medio de divulgación: Papel
          ISSN: 15671348
          DOI: 10.1016/j.meegid.2014.04.007
          G12 group A rotavirus (RVA) are currently recognized as a globally emerging genotype and have been described in combination with several P-types. In Brazil, G12 RVA strains have been described in the Southern (2003) and Northern (2008-2010) regions, in combination with the P[9] and P[6] genotype, respectively. To date, few complete genomes of G12 RVA strains have been described (none from Brazilian strains), considering G12P[9] genotype just one strain, RVA/Human-tc/THA/T152/1998/G12P[9], has their 11 gene segments characterized. This study aims to determine the genomic constellation of G12P[9] and G12P[8] RVA strains detected in Brazil between 2006 and 2011. Therefore, the eleven gene segments of five Brazilian G12 RVA strains were amplified and sequenced, and the genotype of each gene segment was assigned using phylogenetic analysis. Complete genome analyses of G12 RVA strain circulating between 2006 and 2011 in Brazil revealed a conserved Wa-like genomic constellation for three G12P[8] RVA strains; whereas the two G12P[9] strains possessed distinct reassorted AU-1-like genomic constellations, closely related to the reference strain RVA/Human-tc/THA/T152/1998/G12P[9] in most genes. The results obtained in the current study suggest that G12P[9] (AU-1-like) and G12P[8] (Wa-like) strains detected in different regions of Brazil do not share a common origin. Moreover, while Brazilian G12P[8] RVA strains showed a complete Wa-like human constellation, both G12P[9] strains possessed an NSP1 gene of bovine origin (NSP1), and RVA/Human-wt/BRA/PE18974/2010/G12P[9] also possessed a VP3 gene of canine/feline origin.

        • A decade of G3P[8] and G9P[8] rotaviruses in Brazil: epidemiology and evolutionary analyses. (Completo, 2014)
          Gomez MM , Carvalho-Costa FA , Volotao EM , Rose TL , da Silva MFM , Fialho AM , de Assis RM , Matthijnssens J , Leite JPG
          Infection, Genetics and Evolution, v.: 28 p.:389 - 397, 2014
          Palabras clave: Diarrheal disease Genotypes G3 and G9 Group A rotaviruses Monovalent vaccine Phylogenetic analysis
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus de la especie A
          Medio de divulgación: Papel
          ISSN: 15671348
          DOI: 10.1016/j.meegid.2014.05.016
          This study aims to estimate the frequency of group A rotaviruses (RVA) infection with genotypes G3P[8] and G9P[8] in children that suffered from diarrheal disease (DD) between 2001 and 2011 in different Brazilian regions. In addition, the genetic diversity of G3P[8] and G9P[8] RVA strains recovered from vaccinated and non-vaccinated children was assessed. Laboratory-based RVA surveillance included 15,115 cases of DD, and RVA was detected by enzyme immune-assay and/or polyacrylamide gel electrophoresis in 3357 (22%) samples. RVA was genotyped by the semi-nested RT-PCR and among RVA-positive samples, 100 (2.9%) were G3 (63 G3P[8], 32 G3P not typed [NT], and 5 G3P[6]) and 378 (16.2%) were G9 (318 G9P[8], 59 G9P[NT], and 1 G9P[6]). From the G3 and G9 positive samples, 16 and 12, respectively, were obtained from children aged 4-48months vaccinated with the monovalent vaccine (Rotarix®, RV1). Phylogenetic analyses of the VP7 and VP8(?) encoding genes were performed for 26 G3P[8] and 48 G9P[8] strains. VP8(?) phylogenetic analysis revealed that all strains analyzed belonged to P[8] lineage III, whereas RV1 belongs to P[8]-I lineage. VP7 analysis revealed that all G3 and G9 strains belonged to G3-lineage III and G9-lineage III. The comparison of the VP7 and VP8(?) antigenic epitopes regions of Brazilian strains with RV1 strain revealed several amino acid changes. However, no particular differences among Brazilian strains detected before and after vaccine introduction were observed, or among strains detected from vaccinated and non-vaccinated children. Complete genome characterization of four G3P[8] and seven G9P[8] strains revealed a typical conserved human Wa-like genomic constellation. Changes in the genetic diversity of G3P[8] and G9P[8] RVA detected from 2001 to 2011 in Brazil seemed not be related to RV1 introduction in Brazil.

        • VP8*P[8] lineages of group A rotaviruses circulating over 20 years in Brazil: proposal of six different sub-lineages for P[8]-3 clade. (Completo, 2013)
          DA SILVA MFM , Gomez MM , Rose TL , Volotao EM , Carvalho-Costa FA , Bello G , Leite JPG
          Infection, Genetics and Evolution, v.: 16 p.:200 - 205, 2013
          Palabras clave: Rotavírus genetic diversity P[8]
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
          Medio de divulgación: Internet
          ISSN: 15671348
          DOI: 10.1016/j.meegid.2013.01.004
          Group A rotaviruses (RVA) is the most important cause of severe gastroenteritis among children worldwide. Vaccination is considered the best alternative among public health measures to reduce and prevent the global burden caused by RVA infections. Rotarix?, a monovalent vaccine based on a human strain with a G1P[8]-1 specificity, was introduced in the National Brazilian Immunization Programs (NIP) in March, 2006. RVA P[8] is the most prevalent P genotype worldwide and four distinct phylogenetic lineages: P[8]-1, -2, -3, and -4 have been described. In the current study phylogenetic analysis of the VP8(*) gene of 135 RVA P[8] Brazilian strains, in combination with G1, G3, G5 or G9 VP7 genotype, collected from 1986 to 2011 were carried out for a better understanding of the evolution of this viral genotype in Brazil. Lineages P[8]-1, P[8]-2, and P[8]-3 were observed circulating in Brazil. In 2001 these three P[8] lineages co-circulated simultaneously and this is the first report in South America to date. Considering the P[8] lineage and the G genotype, all G3 strains were related to lineage P[8]-3, whereas the G9 strains were related to P[8]-2 and P[8]-3 and G1 and G5 were related to P[8]-1, P[8]-2, and P[8]-3. In addition, the phylogenetic analysis based on estimate of genetic distances between P[8]-3 strains and the definition of a 1.5% cutoff value (with relevant statistical support) it was possible to propose a new classification for the P[8]-3 lineage into six different sub-lineages: P[8]-3.1 to P[8]-3.6. These findings reinforce the notion of the existence of constraints within specific RVA strains populations. The results obtained in this study reinforce the importance of a continuous RVA surveillance of circulating strains in order to predict the possible variants that will circulate in a country, assess the effects of vaccination on RVA circulating strains, and ultimately help in the design, challenges, and prospects of RVA vaccines.

        • Phylogenetic analysis of G1P[6] group A rotavirus strains detected in Northeast Brazilian children fully vaccinated with Rotarix (Completo, 2013)Trabajo relevante
          Gomez MM , DA SILVA MF , ZELLER M , HEYLEN E , MATTHIJNSSENS J , ICHIHARA MY , ROSE TL , VOLOTAO EM , LEITE JPG
          Infection, Genetics and Evolution, v.: 19 p.:395 - 402, 2013
          Palabras clave: Rotavírus Brazil G1P[6] Phylogeny
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
          Medio de divulgación: Internet
          ISSN: 15671348
          DOI: 10.1016/j.meegid.2013.03.028
          In 2009 the World Health Organization recommended the use of group A rotavirus (RVA) vaccines in all national immunization programs (NIPs) in order to control severe RVA gastroenteritis disease. In Brazil, Rotarix? was introduced in the NIP in March 2006, and a significant reduction in mortality rates among children ? 5 years old was observed, especially in the Northern and Northeastern Brazil. In the current study the 11 gene segments of six Brazilian G1P[6] RVA strains, isolated in 2009 and 2010 from vaccinated children, were analyzed in order to investigate if the genetic composition of these strains might help to elucidate why they were able to cause acute gastroenteritis in vaccinated children. All six Brazilian RVA strains revealed a complete Wa-like genotype constellation: G1-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1. Phylogenetic analysis showed that all six strains were nearly identical and showed a close genetic relationship with contemporary typical human Wa-like RVA strains. These results suggests that the fact that these strains were able to cause acute gastroenteritis in vaccinated children is likely not due to the genetic background of the strains, but rather to other factors such as host relating factors, co-infecting pathogens or vaccine efficacy. P[6] RVA strains are detected rather occasionally in humans in most regions of the world, except for South Asia and Sub-Saharan Africa. However, recently two studies conducted in Brazil showed the circulation of G12P[6] and G2P[6]. This is the first report on the detection and complete genome analyses of G1P[6] RVA strains in Brazil. Surveillance studies will be crucial to further investigate the prevalence of this genotype in the Brazilian population, and the efficacy of current licensed vaccines, which do not contain the P[6] genotype.

        • Human G9P[8] rotavirus strains circulating in Cameroon, 1999-2000: Genetic relationships with other G9 strains and detection of a new G9 subtype. (Completo, 2013)
          ESONA MD , MIJATOVIC-RUSTEMPASIC S , FOYTICH K , ROY S , BANYAI K , ARMAH GE , STEELE AD , VOLOTAO EM , Gomez MM , da Silva MFM , Gautam R , Quaye O , Tam KI , Forbi JC , Seheri M , Page N , Nyangao J , Ndze VN , Aminu M , Bowen MD , Gentsch JR
          Infection, Genetics and Evolution, v.: 18 p.:315 - 324, 2013
          Palabras clave: Rotavírus genetic diversity G9P[8]
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
          Medio de divulgación: Internet
          ISSN: 15671348
          DOI: 10.1016/j.meegid.2013.06.005
          Group A rotaviruses (RV-A) are the leading cause of viral gastroenteritis in children worldwide and genotype G9P[8] is one of the five most common genotypes detected in humans. In order to gain insight into the degree of genetic variability of G9P[8] strains circulating in Cameroon, stool samples were collected during the 1999-2000 rotavirus season in two different geographic regions in Cameroon (Southwest and Western Regions). By RT-PCR, 15 G9P[8] strains (15/89=16.8%) were identified whose genomic configurations was subsequently determined by complete or partial gene sequencing. In general, all Cameroonian G9 strains clustered into current globally-spread sublineages of the VP7 gene and displayed 86.6-100% nucleotide identity amongst themselves and 81.2-99.5% nucleotide identity with global G9 strains. The full genome classification of all Cameroonian strains was G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 but phylogenetic analysis of each gene revealed that the strains were spread across 4 or more distinct lineages. An unusual strain, RVA/Human-wt/CMR/6788/1999/G9P[8], which shared the genomic constellation of other Cameroonian G9P[8] strains, contained a novel G9 subtype which diverged significantly (18.8% nucleotide and 19% amino acid distance) from previously described G9 strains. Nucleotide and amino acid alignments revealed that the 3' end of this gene is highly divergent from other G9 VP7 genes suggesting that it arose through extensive accumulation of point mutations. The results of this study demonstrate that diverse G9 strains circulated in Cameroon during 1999-2000.

        • Evidence of vaccine-related reassortment of rotavirus, Brazil, 2008-2010. (Completo, 2013)Trabajo relevante
          ROSE TL , DA SILVA MFM , Gomez MM , Resque HR , Ichihara MY , Volotao EM , Leite JPG
          Emerging Infectious Diseases, v.: 19 11 , p.:1843 - 1846, 2013
          Palabras clave: Rotavírus Rotarix reassortment
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
          Medio de divulgación: Internet
          ISSN: 10806040
          DOI: 10.3201/eid1911.121407
          Analysis of 27 rotavirus strains from vaccinated and unvaccinated children revealed reassortment events in 3 strains: a gene derived from a vaccine; a gene acquired from a circulating strain; and reassortment between circulating strains. Data suggest that the widespread use of this monovalent rotavirus vaccine may introduce vaccine genes into circulating human rotaviruses or vice versa.

        • Rotavirus A genotype P[4]G2: genetic diversity and reassortment events among strains circulating in Brazil between 2005 and 2009. (Completo, 2011)Trabajo relevante
          Gomez MM , Mendonça MCL , Volotao EM , Tort, LFL; Tort FL; Lopez F , da Silva MFM , CRISTINA, J. , Leite JPG
          Journal of Medical Virology, v.: 83 6 , p.:1093 - 1106, 2011
          Palabras clave: Rotavírus Brazil reassortment
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
          Medio de divulgación: Internet
          Lugar de publicación: 10.1002/jmv.22071
          ISSN: 01466615
          DOI: 10.1002/jmv.22071
          Group A rotaviruses (RV-A) are the leading cause of severe gastroenteritis in infants and young children worldwide. Due to the epidemiologic complexity of RV-A, especially in developing countries, it is important to determine which genotypes are circulating, principally after the introduction in March 2006 of the monovalent (P[8]G1) Rotarix® vaccine in Brazil by the National Immunization Program. In Phase III trials with Rotarix®, the prevalence of genotype P[4]G2 was extremely low, and therefore, evaluation of heterotypic immunization against this genotype was performed by meta-analysis statistics tests. Different studies have shown the re-emergence of genotype P[4]G2 in Brazil, since 2005, as well as in other countries, suggesting that it could be a continental phenomenon related to the temporal variability in the genotype's naturally occurring distribution. It is important to note that genotype P[4]G2 does not share VP4 or VP7 antigens with the vaccine strain. Therefore, we performed a phylogenetic analysis based on VP4 (VP8), VP7, VP6, and NSP4 genes of RV-A genotype P[4]G2 samples isolated from the five regions of Brazil between 2005 and 2009. This study revealed that different genetic variants of RV-A genotype P[4]G2 circulated in Brazil between 2005 and 2009, and that this variability is determined mainly by: occurrence of point mutations; reassortment events; and widespread global gene flow. The results obtained in this study are important to our understanding of the epidemiology and evolution of RV-A genotype P[4]G2 and demonstrate the importance of continuous monitoring and molecular characterization of RV-A strains circulating in human and animal populations.

        • Analysis of human P[4]G2 rotavirus strains isolated in Brazil reveals codon usage bias and strong compositional constraints. (Completo, 2011)
          Gomez MM , Tort, LFL; Tort FL; Lopez F , VOLOTAO EM , RECAREY, R. , MORATORIO, G. , MUSTO H , LEITE JPG , CRISTINA, J.
          Infection, Genetics and Evolution, v.: 11 3 , p.:580 - 586, 2011
          Palabras clave: Rotavírus Brazil G2P[4] codon usage
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
          Medio de divulgación: Internet
          ISSN: 15671348
          DOI: 10.1016/j.meegid.2011.01.006
          The Rotavirus genus belongs to the family Reoviridae and its genome consist of 11 segments of double-stranded RNA. Group A rotaviruses (RV-A) are the main etiological agent of acute viral gastroenteritis in infants and young children worldwide. Understanding the extent and causes of biases in codon usage is essential to the understanding of viral evolution. However, the factors shaping synonymous codon usage bias and nucleotide composition in human RV-A are currently unknown. In order to gain insight into these matters, we analyzed the codon usage and base composition constraints on the two genes that codify the two outer capsid proteins (VP4 [VP8*] and VP7) of 58 P[4]G2 RV-A strains isolated in Brazil and investigated the possible key evolutionary determinants of codon usage bias. The results of these studies revealed that the frequencies of codon usage in both RV-A proteins studied are significantly different than the ones used by human cells. In order to observe if similar trends of codon usage are found when RV-A complete genomes are considered, we compare these results with results found using a dataset of 10 reference strains for whom the complete codes of the 11 segments are known. Similar results were obtained using capsid proteins or complete genomes. The general correlations found between the position of each sequence on the first axis generated by correspondence analysis and the relative dinucleotide abundances indicate that codon usage in RV-A can also be strongly influenced by underlying biases in dinucleotide frequencies. CpG and GpC containing codons are markedly suppressed. Thus, the results of this study suggest that RV-A genomic biases are the result of the evolution of genome composition in relation to host adaptation and the ability to escape antiviral cell responses.

        • Laboratory-based rotavirus surveillance during the introduction of a vaccination program, Brazil, 2005-2009. (Completo, 2011)
          Carvalho-Costa FA , Eduardo de Mello Volotao , de Assis RM , Fialho AM , de Andrade J da S , Rocha LN , Tort, LFL; Tort FL; Lopez F , DA SILVA MFM , Gomez MM , de Souza PM , Leite JPG
          Journal of Pediatric Infectious Diseases, v.: 30 p.:35 - 41, 2011
          Palabras clave: Rotavírus Brazil epidemiology
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
          Medio de divulgación: Internet
          ISSN: 18710336
          DOI: 10.1016/j.virusres.2011.07.015
          BACKGROUND: : Brazil introduced universal antirotavirus vaccination in March 2006. This article reports the results of rotavirus A (RV-A) surveillance from January 2005 to December 2009. METHODS: : A total of 6109 fecal samples were collected in 18 Brazilian states. RV-A was detected by enzyme immunoassay and/or polyacrylamide gel electrophoresis, and genotyped through seminested reverse transcription-polymerase chain reaction. RESULTS: : RV-A was detected in 20.3% (n = 1242) of the samples. Among children less than 2 years old, regardless the antirotavirus vaccination status, the rates of RV-A detection were 33.8% in 2005, 23.7% in 2006, 16.8% in 2007, 22.9% in 2008, and 18.3% in 2009 (P < 0.001; ? test for linear trend). Among RV-A-positive samples, genotype G1P[8] or G1P[not typed(NT)] was detected in 14% in 2005, 12.3% in 2006, 9.5% in 2007, 0.7% in 2008, and 20.4% in 2009; G2P[4]/G2P[NT] was characterized in 9% in 2005, 49% in 2006, 66% in 2007, 85% in 2008, and 37.5% in 2009; G3P[8]/G3P[NT] was observed in 8.7% in 2005, 3.5% in 2006, and 5.7% in 2009; G9P[8]/G9P[NT] was observed in 52% in 2005, 22% in 2006, 12.3% in 2007, 3.2% in 2008, and 3.4% in 2009. CONCLUSIONS: : Our data demonstrate the reemergence of RV-A genotype G2P[4] in Brazil from 2005 to 2008, and that the rate of G2P[4] detection decreased in 2009, probably reflecting natural oscillations of RV-A genotypes.

        • VP7 Gene of human rotavirus A genotype G5: Phylogenetic analysis reveals the existence of three different lineages worldwide. (Completo, 2011)
          DA SILVA MF , Tort, LFL; Tort FL; Lopez F , Gomez MM , Assis RM , Volotao EM , de Mendonça MCL , Bello G , Leite JPG
          Journal of Medical Virology, v.: 83 2 , p.:357 - 366, 2011
          Palabras clave: Rotavírus G5 genetic diversity
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
          Medio de divulgación: Internet
          ISSN: 01466615
          DOI: 10.1002/jmv.21968
          Group A rotavirus (RV-A) genotype G5, which is common in pigs, was also detected in children with severe diarrhea in Brazil, Argentina, Paraguay, Cameroon, China, Thailand, and Vietnam. To evaluate the evolutionary relationship among RV-A G5 strains, the VP7 and VP4 genes of 28 Brazilian RV-A G5 human strains, sampled between 1986 and 2005, were sequenced and compared with other RV-A G5 strains currently circulating worldwide in animals and humans. The phylogenetic analysis of RV-A G5 VP7 gene strains demonstrates the existence of three main lineages: (a) Lineage I: Brazilian strains grouped with three porcine strains from Thailand; (b) Lineage II: porcine, bovine, and equine strains from different regions; (c) Lineage III: human strains isolated in Asia and Africa, and two porcine strains from Argentina. The VP8* (*non-typable) subunit of VP4 gene sequencing showed that all P[8] strains fell into three major genetic lineages: P[8]-1; P[8]-2; and P[8]-3. These results showed that the RV-A G5 strains circulating in humans are the result of two independent zoonotic transmission events, most likely from pigs.

        • Phylogenetic analysis of VP1, VP2, and VP3 gene segments of genotype G5 group A rotavirus strains circulating in Brazil between 1986 and 2005. (Completo, 2011)
          DA SILVA MF , TORT LFL , Gomez MM , de Assis RM , de Mendonça MCL , Volotao EM , Leite JPG
          Virus Research, v.: 160 p.:381 - 388, 2011
          Palabras clave: Brazil G5 Phylogenetics
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
          Medio de divulgación: Internet
          ISSN: 01681702
          DOI: 10.1016/j.virusres.2011.07.015
          Genotype G5 group A rotavirus (RV-A), which is common in pigs and also detected in horses and cattle, circulated as endemic genotype in the 1980s and early 1990s in Brazil. After 1996, G5 RV-A has been replaced by G9 RV-A, becoming only sporadically detected. Recently, G5 has been reported in children with severe diarrhea in Argentina, Cameroon, Paraguay, People's Republic of China, and Vietnam, suggesting that, although uncommon in humans, it has a worldwide distribution. In a previous study, Brazilian G5 RV-A VP7 gene analysis demonstrated the existence of three main lineages: I, II, and III; all Brazilian strains and three porcine strains from Thailand grouped inside Lineage I. The VP8(*) subunit of VP4 gene showed that all P[8] strains fell into three major genetic lineages: P[8]-1; P[8]-2; and P[8]-3. Partial sequencing and phylogenetic analysis of VP1, VP2 and VP3 genes of P[8]G5 human RV-A strains were determined from 28 Brazilian strains collected from 1986 to 2005. The VP1-VP3 partial sequences analysis showed that the Brazilian strains have high amino acid identity with the human RV-A prototype IAL28 and other Wa-like genogroup strains. It was also shown that G5 RV-A Brazilian VP1-VP3 and VP7 sequences have a similar pattern of gouping: The study strains and the G5 prototype strain IAL-28 grouped together, while other prototypes, like OSU grouped separately. These results suggest that the core protein genes (VP1-VP3) of the G5 RV-A Brazilian strains might have originated from porcine and human strains. Phylogenetic analyses of VP7, VP4, VP1, VP2, and VP3 genes of P[8]G5 strains revealed a conserved genomic constellation (G5-P[8]-R1-C1-M1) with sequence similarity to Wa-like strains: IAL28, Wa, BE00048, CK00032, CK00033, DC4772 and DC1898, suggesting that despite the differences in genotypes (i.e., G5, G1 and G3) these viruses are genetically similar. The results presented here are fundamental to understand the epidemiology and evolution of genotype G5 RV-A and demonstrate the importance of continuous monitoring and molecular characterization of RV-A strains circulating in human and animal populations.

        • Detection of uncommon rotavirus A strains P[8]G8 and P[4]G8 in the city of Rio de Janeiro, 2002. (Completo, 2010)
          Gomez MM , DE MENDONçA MC , VOLOTãO E DE M , Tort, LFL; Tort FL; Lopez F , DA SILVA MF , CRISTINA, J. , LEITE JPG
          Journal of Medical Virology, v.: 82 7 , p.:1272 - 1276, 2010
          Palabras clave: Rotavírus genetic characterization G8 Rio de Janeiro
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
          Medio de divulgación: Internet
          ISSN: 01466615
          DOI: 10.1002/jmv.21769.
          Group A rotaviruses (RV-A) are the major cause of gastroenteritis in infants and young children around the world. Each year RV-A causes approximately 11 million episodes of severe diarrhea, with an estimated of 611,000 deaths. Epidemiologic surveys have identified P[8]G1, P[4]G2, P[8]G3, P[8]G4, and P[8]G9 as the most common global genotypes associated with diarrhea in children up to 5-year old. Surveillance studies and documentation of RV-A G and P genotypes is necessary for a comprehensive evaluation of the evolution of new strains, and assessing the capability of vaccines to provide heterotypic protection. It is known that reassortments are the driving force for genetic diversity through sudden changes in RV-A genome. In this study, we identified two unusual P/G combinations, P[8]G8 and P[4]G8, occurring in Rio de Janeiro during 2002. Results obtained in this study suggest that P[8]G8 RV-A strain originated from a reassortment event that occurred between RV-A P[4]G8 and P[8]G9 strains circulating in Rio de Janeiro in the same year. G8 strains identified in this study, as well as G8 strains detected in Recife by Montenegro et al. [Montenegro et al. (2007) J Med Virol 79: 335-340], showed a close genetic relationship with strains from Africa, where this genotype have become prevalent.

        • Evolution of naturally 5´ non-coding region variants of hepatitis C virus in human populations of South American region. (Completo, 2007)Trabajo relevante
          MORATORIO, G. , Gomez MM , GUTIERREZ, M. F. , González K , COLINA, R. , Tort, LFL; Tort FL; Lopez F , López L , RECAREY, R. , Schijman AG , Moreno MP , García-Aguirre L , Manascero AR , CRISTINA, J.
          Virology Journal, v.: 4 p.:79 2007
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
          Medio de divulgación: Internet
          ISSN: 1743422X
          DOI: 10.1186/1743-422X-4-79
          www.virologyj.com
          BACKGROUND: Hepatitis C virus (HCV) has been the subject of intense research and clinical investigation as its major role in human disease has emerged. Previous and recent studies have suggested a diversification of type 1 HCV in the South American region. The degree of genetic variation among HCV strains circulating in Bolivia and Colombia is currently unknown. In order to get insight into these matters, we performed a phylogenetic analysis of HCV 5' non-coding region (5'NCR) sequences from strains isolated in Bolivia, Colombia and Uruguay, as well as available comparable sequences of HCV strains isolated in South America. METHODS: Phylogenetic tree analysis was performed using the neighbor-joining method under a matrix of genetic distances established under the Kimura-two parameter model. Signature pattern analysis, which identifies particular sites in nucleic acid alignments of variable sequences that are distinctly representative relative to a background set, was performed using the method of Korber & Myers, as implemented in the VESPA program. Prediction of RNA secondary structures was done by the method of Zuker & Turner, as implemented in the mfold program. RESULTS: Phylogenetic tree analysis of HCV strains isolated in the South American region revealed the presence of a distinct genetic lineage inside genotype 1. Signature pattern analysis revealed that the presence of this lineage is consistent with the presence of a sequence signature in the 5'NCR of HCV strains isolated in South America. Comparisons of these results with the ones found for Europe or North America revealed that this sequence signature is characteristic of the South American region. CONCLUSION: Phylogentic analysis revealed the presence of a sequence signature in the 5'NCR of type 1 HCV strains isolated in South America. This signature is frequent enough in type 1 HCV populations circulating South America to be detected in a phylogenetic tree analysis as a distinct type 1 sub-population. The coexistence of distinct type 1 HCV subpopulations is consistent with quasispecies dynamics, and suggests that multiple coexisting subpopulations may allow the virus to adapt to its human host populations.

        • Phylogenetic analysis of the NS5 gene of dengue viruses isolated in Ecuador (Completo, 2007)
          REGATO, M , RECAREY, R. , MORATORIO, G. , DE MORA D , GARCIA-AGUIRRE L , GóNZALEZ M , MOSQUERA C , ALAVA A , FAJARDO A. , ALVAREZ M , D' Andrea L , DUBRA, A. , Khan B , Gomez MM , CRISTINA, J.
          Virus Research, v.: 132 p.:197 - 200, 2007
          Palabras clave: Dengue vírus NS5 gene Evolution Ecuador
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
          Medio de divulgación: Papel
          ISSN: 01681702
          DOI: 10.1016/j.virusres.2007.10.012
          Dengue virus (DENV) is a member of the genus Flavivirus of the family Flaviviridae. DENV causes a wide range of diseases in humans, from the acute febrile illness dengue fever (DF) to life-threatening dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). There is not knowledge of the genetic relations among DENV circulating in Ecuador. Given the emerging behaviour of DENV, a single tube RT-PCR assay using a pair of consensus primers to target the NS5 coding region has been recently validated for rapid detection of flaviviruses. In order to gain insight into the degree of genetic variation of DENV strains isolated in Ecuador, DENV NS5 sequences from 23 patients were obtained by direct sequencing of PCR fragments using the mentioned one step RT-PCR assay. Phylogenetic analysis carried out using the 23 Ecuadorian DENV NS5 sequences, as well as 56 comparable sequences from DENV strains isolated elsewhere, revealed a close genetic relation among Ecuadorian strains and DENV isolates of Caribbean origin. The use of partial NS5 gene sequences may represent a useful alternative for a rapid phylogenetic analysis of DENV outbreaks.

      • No Arbitrados

        • Diagnóstico rápido del vírus del dengue por analisis molecular. (Completo, 2007)
          MORATORIO, G. , Gomez MM , ALVAREZ M , D ANDREA L , FAJARDO A , DUBRA A , RECAREY, R. , CRISTINA, J.

          Uruguay Ciencia, 2007
          Palabras clave: DENGUE Diagnóstico
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
          Medio de divulgación: Papel
          ISSN: 16883934
  • Libros

    • Virologia Humana e Veterinária ( Participación , 2018)Trabajo relevante
      Gomez MM , Volotão EM , Soares CC
      Edición: 1,
      Editorial: THIEME REVINTER, Rio de Janeiro
      Tipo de puplicación: Divulgación
      En prensa
      Escrito por invitación
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Virus entericos
      Medio de divulgación: Papel
      ISSN/ISBN: 978-85-4565-034-6

      Capítulos:
      Vírus entéricos: Rotavírus, Calicivírus Humanos, Astrovírus e Adenovírus
      Organizadores: Raquel Siqueira de Queiroz Simões
      Página inicial 199, Página final 227
  • Publicación de trabajos presentados en eventos

    • Rotavírus A genótipo G26P[19] em criança hospitalizada com gastroenterite aguda: segunda descrição mundial e caracterização genômica completa (2016)
      Resumen
      Gomez MM , Fialho AM , Assis RMS , Volotao EM , Mazzoco CS , Andrade JSR , Mouta Junior SS , Rocha MS , Leite JPG

      Evento: Internacional
      Descripción: III Simpósio Internacional de Imunobiológicos
      Ciudad: Rio de Janeiro, Brasil.
      Año del evento: 2016
      Anales/Proceedings:Anais do III Simpósio Internacional de Imunobiológicos. Bio-manguinhos
      Pagina inicial: 184
      Pagina final: 185
      Palabras clave: G26P[1]9 rotavirus reassortment rearrangment genomic constellation Brazil
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus
    • Rotavirus A epidemiology one decade after the introducction of universal vaccination with Rotarix in Brazil. (2016)
      Resumen
      Carvalho-Costa FA , Assis RMS , Fialho AM , Araújo IT , da Silva MFM , Gomez MM , Andrade JSR , Volotão EM , Rose TL , Fumian T , Leite JPG

      Evento: Internacional
      Descripción: 12th International Rotavirus Symposium
      Ciudad: Melbourne, Austrália.
      Año del evento: 2016
      Anales/Proceedings:Symposium program book
      Pagina inicial: 100
      Pagina final: 100
      Palabras clave: rotavirus rotarix epidemiology
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus
    • Genomic constellation of a porcine-like human G26P[19] rotavirus strain isolated from a child with acute gastroenteritis in Rio de Janeiro in 2015 (2016)
      Resumen
      Gomez MM , Fialho AM , Assis RMS , Volotao EM , Mazzoco CS , Andrade JSR , Mouta Junior SS , Rocha SM

      Evento: Internacional
      Descripción: 12th International Rotavirus Symposium
      Ciudad: Melbourne, Austrália.
      Año del evento: 2016
      Anales/Proceedings:Symposium program book
      Pagina inicial: 101
      Pagina final: 101
      Palabras clave: rotavirus G26P[19] GENOMIC CONSTELLATION REASSORTMENT REARRANGMENT
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus
    • Epidemiology of group A rotaviruses in Brazil, 1996-2013: impact of universal vaccination with the monovalent vaccine. (2015)
      Resumen
      Carvalho-Costa FA , Assis RMS , Fialho AM , Araújo IT , da Silva MFM , Gomez MM , Andrade JSR , Rose TL , Nobrec AA , Volotão EM , Barreto ML , Leite JPG

      Evento: Internacional
      Descripción: 4th European expert meeting on rotavirus vaccination - EEROVAC
      Ciudad: Santiago de Compostela, España.
      Año del evento: 2015
      Anales/Proceedings:Abstract book
      Pagina inicial: 21
      Pagina final: 21
      Palabras clave: rotavirus Brazil epidemiology
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Caracterización molecular de cepas de astrovirus humano emergente MLB-1 em agua residual de Uruguay. (2015)
      Resumen
      LIZASOAIN, A. , Tort, LFL; Tort FL; Lopez F , Garcia M , Gomez MM , Leite JPG , CRISTINA, J. , Colina R , VICTORIA M

      Evento: Internacional
      Descripción: XI Congreso Argentino de Virología, II Congreso Latinoamericano de Virología, IV Simposio de Virología Clínica, II Simposio de Virología Veterinaria
      Ciudad: Buenos Aires, Argentina.
      Año del evento: 2015
      Anales/Proceedings:Libro de resúmenes
      Pagina inicial: 146
      Pagina final: 146
      Palabras clave: astrovirus agua residual Uruguay caracterización molecular
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Virologia Ambiental
    • Presencia de norovirus y astrovirus canino em aguas residuales de Uruguay. (2015)
      Resumen
      LIZASOAIN, A. , Tort, LFL; Tort FL; Lopez F , Garcia M , Gomez MM , Leite JPG , Miagostovich MP , CRISTINA, J. , Colina R , VICTORIA M

      Evento: Internacional
      Descripción: XI Congreso Argentino de Virología, II Congreso Latinoamericano de Virología, IV Simposio de Virología Clínica, II Simposio de Virología Veterinaria
      Ciudad: Buenos Aires, Argentina.
      Año del evento: 2015
      Anales/Proceedings:Libro de resúmenes
      Pagina inicial: 148
      Pagina final: 148
      Palabras clave: norovirus astrovirus aguas residuales Uruguay detección
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Virologia Ambiental
    • Detection of common, emerging and uncommon VP4 and VP7 human group A rotavirus genotypes from urban sewage samples in Uruguay. (2015)
      Resumen
      Tort, LFL; Tort FL; Lopez F , VICTORIA M , LIZASOAIN, A. , Berois M , CRISTINA, J. , Leite JPG , Gomez MM , Miagostovich MP , Colina R

      Evento: Internacional
      Descripción: XXVI Congresso Brasileiro de Virologia, X Encontro de Virologia do Mercosul.
      Ciudad: Florianópolis, Santa Catarina, Brasil.
      Año del evento: 2015
      Anales/Proceedings:Virus Reviews and Research
      Volumen:20
      Pagina inicial: 30
      Pagina final: 31
      Palabras clave: rotavirus sewage Uruguay
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Virologia Ambiental
    • Identification of canine norovirus ans astrovirus in sewage from Uruguay. (2015)
      Resumen
      LIZASOAIN, A. , Tort, LFL; Tort FL; Lopez F , Garcia M , Gomez MM , Leite JPG , Miagostovich MP , CRISTINA, J. , Colina R

      Evento: Internacional
      Descripción: XXVI Congresso Brasileiro de Virologia, X Encontro de Virologia do Mercosul
      Ciudad: Florianópolis, Santa Catarina, Brasil.
      Año del evento: 2015
      Anales/Proceedings:Virus Reviews and Research
      Volumen:20
      Pagina inicial: 84
      Pagina final: 84
      Palabras clave: canine norovirus sewage Uruguay astrovirus
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Virologia Ambiental
    • The changing epidemiology of rotavirus diarrhea in the context of universal vaccination in Brazil. (2014)
      Resumen
      Gomez MM , Carvalho-Costa FA , Volotão EM , Rose TL , da Silva MFM , Fialho AM , Assis RMS , Matthijnssens J , Leite JPG

      Evento: Internacional
      Descripción: 11th International Rotavirus Symposium
      Año del evento: 2014
      Anales/Proceedings:Abstract book
      Pagina inicial: 132
      Pagina final: 133
      Palabras clave: rotavirus epidemiology Brazil
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • A decade of G3P[8] and G9P[8] rotaviruses in Brazil: Epidemiology and evolutionary analyses. (2014)
      Resumen
      Gomez MM , Carvalho-Costa FA , Volotão EM , Rose TL , da Silva MFM , Fialho AM , Assis RMS , Leite JPG

      Evento: Internacional
      Descripción: 11th International Rotavirus Symposium
      Ciudad: Nova Deli, Índia.
      Año del evento: 2014
      Anales/Proceedings:Abstract book
      Pagina inicial: 133
      Pagina final: 134
      Palabras clave: rotavirus G3 G9 molecular epidemiology Brazil
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Epidemiology of group A rotavirus diarrhea in the context of universal monovalent (G1P[8]) vaccination in Brazil (2014)
      Resumen
      Volotao EM , Carvalho-Costa FA , Gomez MM , da Silva MFM , Rose TL , Fialho AM , Assis RMS , Leite JPG

      Evento: Internacional
      Descripción: II Seminário Anual Científico e Tecnológico em Imunobiológicos
      Año del evento: 2014
      Palabras clave: rotavirus epidemiology Brazil
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Constelação gênica dos genótipos: Wa-like/DS-1-like/AU-1-like, de Rotavírus do grupo A antes e após a introdução da vacina monovalente (G1P[8]) no Brasil. (2014)
      Resumen
      Rose TL , Gomez MM , Carvalho-Costa FA , Volotão EM , da Silva MFM , Resque HR , Fialho AM , Assis RMS , Leite JPG

      Evento: Internacional
      Descripción: II Seminário Anual Científico e Tecnológico em Imunobiológicos
      Año del evento: 2014
      Palabras clave: constelação gênica Brazil rotavirus
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Laboratory-based rotavirus surveillance during the vaccination program, Brazil, 2010-2012. (2013)
      Resumen
      Carvalho-Costa FA , Volotão EM , Assis RMS , Gomez MM , Fialho AM , Andrade J , Hugo R , da Silva MFM , Rose TL , Leite JPG

      Evento: Internacional
      Descripción: 5th European Rotavirus Biology Meeting
      Año del evento: 2013
      Anales/Proceedings:Abstract book
      Pagina inicial: 69
      Pagina final: 69
      Palabras clave: rotavirus epidemiology Brazil
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Genome sequence analysis of six Brazilian human rotaviruses provides evidence for mammalian interspecies transmission. (2013)
      Resumen
      Rose TL , Gomez MM , Fialho AM , Volotão EM , da Silva MFM , Leite JPG

      Evento: Internacional
      Descripción: 5th European Rotavirus Biology Meeting
      Año del evento: 2013
      Anales/Proceedings:Abstract book
      Pagina inicial: 69
      Pagina final: 70
      Palabras clave: rotavirus reassortment Brazil
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Laboratory-based rotavirus surveillance during the vaccination program, Brazil, 2010-2012. (2013)
      Resumen
      Carvalho-Costa, FA , Leite JPG , Volotão EM , Assis RMS , Fialho AM , Andrade JSR , Resque HR , Silva MFM , Gomez MM , Rose TL

      Evento: Internacional
      Descripción: XXIV Brazilian Congress of Virology & VIII Mercosur Meeting of Virology
      Año del evento: 2013
      Anales/Proceedings:Virus Reviews and Research
      Volumen:18
      Pagina inicial: 25
      Pagina final: 25
      Palabras clave: rotavirus epidemiology Brazil survillance
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • P[8] Lineages of group A rotaviruses circulating over 20 years in Brazil: proposal of a new classification for P[8]-3 sub-lineages. (2012)
      Resumen
      da Silva MFM , Gomez MM , Rose TL , Volotão EM , Bello G , Leite JPG

      Evento: Internacional
      Descripción: 11th International Symposium on Double-Stranded RNA Viruses
      Ciudad: San Juan, Puerto Rico.
      Año del evento: 2012
      Anales/Proceedings:Caderno de resumos
      Pagina inicial: 52
      Pagina final: 52
      Palabras clave: rotavirus Brazil P[8] lineages
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Molecular characterization of VP4 and VP7 from G2P[4] rotavirus A strains detected in vaccinated and non-vaccinated children in Brazil. (2012)
      Resumen
      Gomez MM , da Silva MFM , Carvalho-Costa F , Zeller M , Heylen E , Matthijnssens J , Leite JPG

      Evento: Internacional
      Descripción: 11th International Symposium on Double-Stranded RNA Viruses
      Ciudad: San Juan, Puerto Rico.
      Año del evento: 2012
      Anales/Proceedings:Caderno de resumos
      Pagina inicial: 71
      Pagina final: 71
      Palabras clave: rotavirus G2P[4] molecular epidemiology Brazil reassortment
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Molecular characterization of rotavirus specie a G1P[8] strains from children vaccinated with Rotarix® vaccine (2011)
      Resumen
      Rose TL , da Silva MFM , Gomez MM , Volotao EM , Leite JPG

      Evento: Internacional
      Descripción: XXII Encontro Nacional de Virologia, VI Encontro de Virologia do Mercosul
      Ciudad: Atibaia, São Paulo, Brasil.
      Año del evento: 2011
      Anales/Proceedings:VIRUS Reviews and Research
      Volumen:16
      Pagina inicial: 175
      Pagina final: 175
      Palabras clave: rotavirus Brazil G1P[8] vaccine
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Phylogenetic analysis of Brazilian P[6]G1 rotavirus A positive samples reveals Rotarix® vaccine escape strains (2011)
      Resumen
      Gomez MM , Rose TL , da Silva MFM , Volotao EM , Costa RA , Leite JPG

      Evento: Internacional
      Descripción: XXII Encontro Nacional de Virologia, VI Encontro de Virologia do Mercosul
      Ciudad: Atibaia, São Paulo, Brasil.
      Año del evento: 2011
      Anales/Proceedings:VIRUS Reviews and Research
      Volumen:16
      Pagina inicial: 186
      Pagina final: 186
      Palabras clave: rotavirus G1P[6] epidemiology Brazil
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Phylogenetic analysis of Brazilian P6G1 strains from children vaccinated with Rotarix®. (2011)
      Resumen
      Gomez MM , Rose TL , da Silva MFM , Volotao EM , Leite JPG

      Evento: Internacional
      Descripción: 4th European Rotavirus Biology Symposium
      Ciudad: Villa San Giovanni, Italia
      Año del evento: 2011
      Anales/Proceedings:Abstract book
      Volumen:4
      Pagina inicial: 43
      Pagina final: 43
      Palabras clave: rotavirus G1P[6] Brazil epidemiology
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Molecular Analysis of VP1, VP2, and VP3 Genes of Group A Rotavirus Genotype G5 Circulating in Brazil between 1986 and 2005. (2011)
      Resumen
      da Silva MFM , Tort, LFL; Tort FL; Lopez F , Gomez MM , Assis RMS , Mendonça MCL , Volotao EM , Leite JPG

      Evento: Internacional
      Descripción: 4th European Rotavirus Biology Symposium
      Ciudad: Villa San Giovanni, Italia
      Año del evento: 2011
      Anales/Proceedings:Abstract book
      Volumen:4
      Pagina inicial: 52
      Pagina final: 52
      Palabras clave: rotavirus G5 Brazil VP1 VP2 VP3
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Genotyping and molecular characterization of VP4, VP7 and NSP4 genes of rotavirus A circulating in Ceará State, Brazil, between 2008 and 2009. (2011)
      Resumen
      Sá AC , Gomez MM , Lima IFN , Quetz JS , Havt A , Oriá RB , Lima AAM , Leite JPG

      Evento: Internacional
      Descripción: X Congreso Argentino de Virologia, III Simposio de Virologia Clinica, I Simposio de virologia veterinaria
      Ciudad: Buenos Aires, Argentina
      Año del evento: 2011
      Anales/Proceedings:REVISTA ARGENTINA DE MICROBIOLOGIA
      Volumen:43
      Pagina inicial: 62
      Pagina final: 63
      Palabras clave: rotavirus Brazil molecular epidemiology
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Phylogenetic analysis of Brazilian P[6]G1 rotavirus A positive samples reveals Rotarix® vaccine escape strains (2011)
      Resumen
      Gomez MM , Rose TL , da Silva MFM , Volotão EM , Costa RA , Leite JPG

      Evento: Internacional
      Descripción: X Congreso Argentino de Virologia, III Simposio de Virologia Clinica, I Simposio de virologia veterinaria
      Ciudad: Buenos Aires, Argentina
      Año del evento: 2011
      Anales/Proceedings:REVISTA ARGENTINA DE MICROBIOLOGIA
      Volumen:43
      Pagina inicial: 98
      Pagina final: 98
      Palabras clave: rotavirus G1P[6] phylogenetic epidemiology
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Genotipos de rotavirus A circulantes en América Latina. (2010)
      Resumen
      Leite JPG , Tort, LFL; Tort FL; Lopez F , Gomez MM , da Silva MFM , Rocha LN , Fialho AM , Assis RMS , Volotao EM

      Evento: Internacional
      Descripción: IX Encuentro Nacional de Microbiólogos
      Ciudad: Montevideo, Uruguay.
      Año del evento: 2010
      Anales/Proceedings:Libro de Resúmenes.
      Pagina inicial: 19
      Pagina final: 19
      Palabras clave: rotavirus genetic diversity Latin America
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Rotavirus A genotype P[4]G2: genetic diversitiy and reassortment events among strains circulating in Brazil between 2005 and 2009 (2010)
      Resumen
      Gomez MM , Mendonça MCL , Volotao EM , Tort, LFL; Tort FL; Lopez F , da Silva MFM , Sá ACC , CRISTINA, J. , Leite JPG

      Evento: Internacional
      Descripción: XXI Encontro Nacional de Virologia. V Encontro de Virologia do Mercosul.
      Ciudad: Gramado, Rio Grande do Sul, Brasil
      Año del evento: 2010
      Anales/Proceedings:Virus Reviews and Research
      Volumen:15
      Pagina inicial: 149
      Pagina final: 149
      Palabras clave: rotavirus G2P[4] genetic diversity reasssortment Brazil
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Genetic diversity and reassortment events among P[4]G2 rotaviurs strains circulating in Brazil between 2005 and 2009. (2010)
      Resumen
      Gomez MM , MendonÇa MCL , Volotao EM , Tort, LFL; Tort FL; Lopez F , da Silva MFM , CRISTINA, J. , Leite JPG

      Evento: Internacional
      Descripción: 9th International Rotavirus Symposium
      Ciudad: Johannesburg, Africa.
      Año del evento: 2010
      Anales/Proceedings:Realizing the impact of Rotavirus Vaccines
      Pagina inicial: 62
      Pagina final: 62
      Palabras clave: rotavirus reassortment epidemiology Brazil
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología /
    • Detection of uncommon rotavirus A strains P[8]G8 and P[4]G8 in the city of Rio de Janeiro, 2002. (2009)
      Resumen
      Gomez MM , Volotao EM , Mendonça MCL , Tort, LFL; Tort FL; Lopez F , da Silva MFM , Leite JPG

      Evento: Internacional
      Descripción: XX Encontro Nacional de Virologia
      Ciudad: Brasilia, Distrito Federal, Brasil
      Año del evento: 2009
      Anales/Proceedings:Virus: Reviews and Research - Journal of the Brazilian Society for Virology.
      Volumen:14
      Pagina inicial: 140
      Pagina final: 140
      Palabras clave: rotavirus Brazil uncommon genotypes
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
    • Study of human rotavirus genotype G3 in isolates from Rio de Janeiro from 1996 to 2006: molecular characterization and comparative analysis. (2009)
      Resumen
      ROCHA LN , da Silva MFM , MENDONçA MCL , Gomez MM , LEITE JPG , VOLOTAO EM

      Evento: Internacional
      Descripción: XX Encontro Nacional de Virologia
      Ciudad: Brasilia, Distrito Federal, Brasil.
      Año del evento: 2009
      Anales/Proceedings:Virus: Reviews and Research - Journal of the Brazilian Society for Virology.
      Volumen:14
      Pagina inicial: 194
      Pagina final: 195
      Palabras clave: rotavirus genetic diversity G3 Brazil
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
    • Rotavirus genotype G9 circulating in Brazil: genes sequences and phylogenetic analysis. (2008)
      Resumen
      Gomez MM , Tort, LFL; Tort FL; Lopez F , Fialho AM , Leite JPG , Volotao EM

      Evento: Internacional
      Descripción: XIX Encontro Nacional de Virologia
      Ciudad: Caxambu, Minas Gerais, Brasil.
      Año del evento: 2008
      Anales/Proceedings:Virus: Reviews and Research - Journal of the Brazilian Society for Virology.
      Volumen:13
      Pagina inicial: 177
      Pagina final: 177
      Palabras clave: rotavirus G9 Brazil epidemiology
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Rotavirus A
  • Producción técnica

    • Otras Producciones

      • Organización de eventos

        • Curso de verão: Doenças virais com importância em saúde pública no Brasil (2008)
          Gomez MM
          Otro
          Sub Tipo: Organización
          Lugar: Brasil ,Instituto Oswaldo Cruz Rio de Janeiro
          Idioma: Portugués
          Institución Promotora/Financiadora: Instituto Oswaldo Cruz, Fundação Oswaldo Cruz
          Areas de conocimiento:
          Ciencias Médicas y de la Salud / Ciencias de la Salud / Ciencias de la Salud /
          Información adicional: Membro da comissão organizadora do curso de verão: Doenças virais com importância em saúde pública no Brasil, Programa de Pós-graduação stricto sensu em Biologia Celular e Molecular do IOC, Fiocruz. Carga horária: 56 horas.
        • Ciência e Tecnologia na América Latina (2006)
          Gomez MM
          Otro
          Sub Tipo: Organización
          Lugar: Brasil ,Universidade Estadual de Campinas (Unicamp) Campinas
          Idioma: Portugués
          Institución Promotora/Financiadora: Universidade Estadual de Campinas (Unicamp) / Asociación de Universidades Grupo Montevideo (AUGM)
          Areas de conocimiento:
          Ciencias Naturales y Exactas / Otras Ciencias Naturales / Otras Ciencias Naturales /
          Información adicional: Participação na organização do 3º Seminário Internacional: ?Ciência e Tecnologia na América Latina?. Unicamp, São Paulo, Brasil.
    • Formación de RRHH

      • Tutorías concluidas

        • Otras

          • Caracterizacao molecular e epidemiologia molecular de rotavirus A (2017)
            Otras tutorías/orientaciones
            Sector Extranjero/Internacional/Otros / Fundação Oswaldo Cruz - Rio de Janeiro , Brasil
            Tipo de orientación: Cotutor en pie de igualdad
            Nombre del orientado: Christian Sagave Mazzocco de Almeida
            País/Idioma: Brasil, Portugués
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
            Programa Institucional de Becas de Iniciación Científica PIBIC- CNPq/FIOCRUZ.
          • Detecção e caracterização genotípica de Rotavírus A em amostras fecais humanas de Fortaleza, Ceará. (2012)
            Otras tutorías/orientaciones
            Sector Extranjero/Internacional/Otros / Instituto Oswaldo Cruz, Fundação Oswaldo Cruz , Brasil
            Tipo de orientación: Asesor/Orientador
            Nombre del orientado: Ana Caroline Costa Sá
            País/Idioma: Brasil, Portugués
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología / Virus Gatsroentéricos
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
          • Caracterizacao molecular de rotavirus A (2012)
            Otras tutorías/orientaciones
            Sector Extranjero/Internacional/Otros / Fundação Oswaldo Cruz - Rio de Janeiro , Brasil
            Tipo de orientación: Cotutor en pie de igualdad
            Nombre del orientado: Júlio César de Araújo Vanelis Soares
            País/Idioma: Brasil, Portugués
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
            Programa Institucional de Becas de Iniciación Científica PIBIC- CNPq/FIOCRUZ.
      • Otros datos relevantes

        • Premios, Honores y Títulos

          • Premio de Tesis Alexandre Peixoto 2015 / Interfarma de Inovação e Pesquisa (2015)
            (Nacional)
            Instituto Oswaldo Cruz - Fiocruz
            El "Premio de Tesis Alexandre Peixoto" fue creado por el Instituto Oswaldo Cruz (IOC / Fiocruz) para contemplar anualmente las mejores tesis desarrolladas por doctorandos de los Programas de Postgrado Stricto sensu del IOC. La premiación es un homenaje al investigador Alexandre Peixoto, fallecido precozmente en febrero de 2013, época en que coordinaba el Programa de Postgrado Stricto sensu en Biología Celular y Molecular del IOC.
          • Beca de Postdoctorado NOTA 10 (2015)
            (Nacional)
            FAPERJ

          • Beca de doctorado NOTA 10 (2012)
            (Nacional)
            FAPERJ

          • Mención de trabajo relevante en la sección de Epidemiologia (2011)
            (Internacional)
            Sociedad Argentina de Virología - SAV / Asociación Argentina de Microbiología - AAM
            Mención de trabajo relevante en la sección de Epidemiologia referente al póster presentado en el " X Congreso Argentino de Virología" (III Simposio de Virología Clínica, I Simposio de virología veterinaria) en Buenos Aires, Argentina. Titulo del poster: Phylogenetic analysis of Brazilian P[6]G1 rotavirus A positive samples reveals Rotarix® vaccine escape strains. Autores: Gómez MM, Rose TL, da Silva MFM, Volotão EM, Costa RA, Leite JPG.
        • Presentaciones en eventos

          • Campanha de conscientização e vacinação da Febre Amarela (2017)
            Otra
            Titulo de la conferencia: "Febre amarela: perguntas e respostas"
            Brasil
            Tipo de participación: Conferencista invitado
            Nombre de la institución promotora: Instituto Federal do Rio de Janeiro (IFRJ)
            Areas de conocimiento:
            Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas / Fiebre Amarilla
          • III Simpósio Internacional de Imunobiológicos. (2016)
            Simposio
            Rotavírus A genótipo G26P[19] em criança hospitalizada com gastroenterite aguda: segunda descrição mundial e caracterização genômica completa .
            Brasil
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular
          • 12th International Rotavirus Symposium, (2016)
            Simposio
            Rotavirus A epidemiology one decade after the introducction of universal vaccination with Rotarix in Brazil.
            Australia
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • 12th International Rotavirus Symposium. (2016)
            Simposio
            Genomic constellation of a porcine-like human G26P[19] rotavirus strain isolated from a child with acute gastroenteritis in Rio de Janeiro in 2015.
            Australia
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XXVII Congresso Brasileiro de Virologia - XI Encontro de Virologia do Mercosul (2016)
            Congreso
            Conferencia administrada sobre epidemiologia de los rotavirus antes y después de la introducción de la vacuna Rotarix en el calendário nacional de vacunación en Brasil
            Brasil
            Tipo de participación: Conferencista invitado
            Nombre de la institución promotora: Sociedade Brasileira de Virologia - SBV
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Epidemiología molecular y evolución de los rotavirus del grupoa A en Brasil
          • 4th European expert meeting on rotavirus vaccination - EEROVAC (2015)
            Congreso
            Epidemiology of group A rotaviruses in Brazil, 1996-2013: impact of universal vaccination with the monovalent vaccine.
            España
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XI Encuentro Nacional de Microbiólogos. (2015)
            Encuentro
            Epidemiologia molecular de rotavirus del grupo A en niños hospitalizados con gastroenterite aguda en Salto, 2011-2012: primera deteccion del gebotipo G12 en el pais.
            Uruguay
            Tipo de participación: Poster
            Nombre de la institución promotora: Sociedad Uruguaya de Microbiología
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XI Encuentro Nacional de Microbiólogos. (2015)
            Encuentro
            Detección de genotipos comunes, emergentes e inusuales de Rotavirus Humanos a partir de muestras de agua residual urbana de Uruguay.
            Uruguay
            Tipo de participación: Poster
            Nombre de la institución promotora: Sociedad Uruguaya de Microbiología.
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XI Congreso Argentino de Virología, II Congreso Latinoamericano de Virología, IV Simposio de Virología Clínica, II Simposio de Virología Veterinaria. (2015)
            Congreso
            Caracterización molecular de cepas de astrovirus humano emergente MLB-1 em agua residual de Uruguay.
            Argentina
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XI Congreso Argentino de Virología, II Congreso Latinoamericano de Virología, IV Simposio de Virología Clínica, II Simposio de Virología Veterinaria, (2015)
            Congreso
            Presencia de norovirus y astrovirus canino em aguas residuales de Uruguay.
            Argentina
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XXVI Congresso Brasileiro de Virologia, X Encontro de Virologia do Mercosul. (2015)
            Congreso
            Detection of common, emerging and uncommon VP4 and VP7 human group A rotavirus genotypes from urban sewage samples in Uruguay.
            Brasil
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XXVI Congresso Brasileiro de Virologia, X Encontro de Virologia do Mercosul. (2015)
            Congreso
            Identification of canine norovirus ans astrovirus in sewage from Uruguay.
            Brasil
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • 11th International Rotavirus Symposium, (2014)
            Simposio
            The changing epidemiology of rotavirus diarrhea in the context of universal vaccination in Brazil.
            India
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • 11th International Rotavirus Symposium, (2014)
            Simposio
            A decade of G3P[8] and G9P[8] rotaviruses in Brazil: Epidemiology and evolutionary analyses.
            India
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • II Seminário Anual Científico e Tecnológico em Imunobiológicos. (2014)
            Seminario
            Epidemiology of group A rotavirus diarrhea in the context of universal monovalent (G1P[8]) vaccination in Brazil.
            Brasil
            Tipo de participación: Poster
            Nombre de la institución promotora: Bio-Manguinhos / Fiocruz
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular
          • II Seminário Anual Científico e Tecnológico em Imunobiológicos. (2014)
            Seminario
            Constelação gênica dos genótipos: Wa-like/DS-1-like/AU-1-like, de Rotavírus do grupo A antes e após a introdução da vacina monovalente (G1P[8]) no Brasil.
            Brasil
            Tipo de participación: Poster
            Nombre de la institución promotora: Bio-Manguinhos / Fiocruz
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular
          • Taller de actualización en la genotipificación de rotavirus (2014)
            Taller
            Conferencia administrada en el Curso Internacional - Taller de actualización en la genotipificación de rotavirus.
            Brasil
            Tipo de participación: Conferencista invitado
            Nombre de la institución promotora: Instituto Oswaldo Cruz / Organización Panamericana de la Salud - OPAS / Organización Mundial de la Salud - OMS.
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Evolución de los rotavirus
          • 5th European Rotavirus Biology Meeting, (2013)
            Encuentro
            Laboratory-based rotavirus surveillance during the vaccination program, Brazil, 2010-2012.
            España
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • 5th European Rotavirus Biology Meeting, (2013)
            Encuentro
            Genome sequence analysis of six Brazilian human rotaviruses provides evidence for mammalian interspecies transmission.
            España
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XXIV Brazilian Congress of Virology & VIII Mercosur Meeting of Virology. (2013)
            Congreso
            Laboratory-based rotavirus surveillance during the vaccination program, Brazil, 2010-2012.
            Brasil
            Tipo de participación: Poster
            Nombre de la institución promotora: Sociedade Brasileira de Virologia - SBV
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • Taller de actualización en la genotipificación de rotavirus (2013)
            Taller
            Conferencia administrada en el Curso Internacional - Taller de actualización en la genotipificación de rotavirus.
            Brasil
            Tipo de participación: Conferencista invitado
            Nombre de la institución promotora: Instituto Oswaldo Cruz / Organización Panamericana de la Salud - OPAS / Organización Mundial de la Salud - OMS.
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Evolución de rotavirus del grupo A
          • 11th International Symposium on Double-Stranded RNA Viruses. (2012)
            Simposio
            P[8] Lineages of group A rotaviruses circulating over 20 years in Brazil: proposal of a new classification for P[8]-3 sub-lineages.
            Puerto Rico
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • 11th International Symposium on Double-Stranded RNA Viruses. (2012)
            Simposio
            Molecular characterization of VP4 and VP7 from G2P[4] rotavirus A strains detected in vaccinated and non-vaccinated children in Brazil.
            Puerto Rico
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • 4th European Rotavirus Biology Meeting (2011)
            Encuentro
            Phylogenetic analysis of Brazilian P6G1 strains from children vaccinated with Rotarix
            Italia
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • 4th European Rotavirus Biology Meeting (2011)
            Encuentro
            Molecular Analysis of VP1, VP2, and VP3 Genes of Group A Rotavirus Genotype G5 Circulating in Brazil between 1986 and 2005
            Italia
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • X Congreso Argentino de Virologia, III Simposio de Virologia Clinica, I Simposio de virologia veterinaria. (2011)
            Congreso
            Genotyping and molecular characterization of VP4, VP7 and NSP4 genes of rotavirus A circulating in Ceará State, Brazil, between 2008 and 2009.
            Argentina
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • X Congreso Argentino de Virologia, III Simposio de Virologia Clinica, I Simposio de virologia veterinaria, (2011)
            Congreso
            Phylogenetic analysis of Brazilian P[6]G1 rotavirus A positive samples reveals Rotarix® vaccine escape strains.
            Argentina
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XXII Encontro Nacional de Virologia, VI Encontro de Virologia do Mercosul (2011)
            Encuentro
            Molecular characterization of rotavirus specie a G1P[8] strains from children vaccinated with Rotarix® vaccine
            Brasil
            Tipo de participación: Poster
            Nombre de la institución promotora: Sociedade Brasileira
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XXII Encontro Nacional de Virologia, VI Encontro de Virologia do Mercosul (2011)
            Encuentro
            Phylogenetic analysis of Brazilian P[6]G1 rotavirus A positive samples reveals Rotarix® vaccine escape strains.
            Brasil
            Tipo de participación: Poster
            Nombre de la institución promotora: Sociedade Brasileira de Virologia - SBV
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XX Congreso Latinoamericano de Microbiología. IX Encuentro Nacional de Microbiólogos (2010)
            Congreso
            Genotipos de rotavirus A circulantes en América Latina.
            Uruguay
            Tipo de participación: Poster
            Nombre de la institución promotora: Sociedad Uruguaya de Microbiología
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XXI Encontro Nacional de Virologia. V Encontro de Virologia do Mercosul (2010)
            Encuentro
            Rotavirus A genotype P[4]G2: genetic diversitiy and reassortment events among strains circulating in Brazil between 2005 and 2009.
            Brasil
            Tipo de participación: Poster
            Nombre de la institución promotora: Sociedade Brasileira de Virologia - SBV
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • 9th International Rotavirus Symposium (2010)
            Simposio
            Genetic diversity and reassortment events among P[4]G2 rotaviurs strains circulating in Brazil between 2005 and 2009.
            Sudáfrica
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XX Encontro Nacional de Virologia (2009)
            Encuentro
            Detection of uncommon rotavirus A strains P[8]G8 and P[4]G8 in the city of Rio de Janeiro, 2002.
            Brasil
            Tipo de participación: Poster
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XX Encontro Nacional de Virologia (2009)
            Encuentro
            Study of human rotavirus genotype G3 in isolates from Rio de Janeiro from 1996 to 2006: molecular characterization and comparative analysis.
            Brasil
            Tipo de participación: Poster
            Nombre de la institución promotora: Sociedade Brasileira de Virologia - SBV
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XIX Encontro Nacional de Virologia (2008)
            Encuentro
            Rotavirus genotype G9 circulating in Brazil: genes sequences and phylogenetic analysis.
            Brasil
            Tipo de participación: Poster
            Nombre de la institución promotora: Sociedade Brasileira de Virologia - SBV
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XIV Jornadas de Jóvenes Investigadores de la AUGM (2006)
            Encuentro
            Presentación oral
            Brasil
            Tipo de participación: Expositor oral
            Nombre de la institución promotora: AUGM
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
            Presentación oral con el título: "Evidencia de diversificación genética en cepas del virus de la Hepatitis C aisladas en Colombia"
          • XIV Jornadas de jovens pesquisadores da AUGM (2006)
            Encuentro
            Evidencia de diversificación genética en cepas del virus de la Hepatitis C aisladas en Colombia.
            Brasil
            Tipo de participación: Expositor oral
            Nombre de la institución promotora: AUGM / Unicamp
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
          • XIV Jornadas de jovens pesquisadores da AUGM (2006)
            Encuentro
            Evidencia de diversificación genética en cepas del virus de la Hepatitis C aisladas en Colombia.
            Brasil
            Tipo de participación: Poster
            Nombre de la institución promotora: AUGM / Unicamp
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Virología
        • Jurado/Integrante de comisiones evaluadoras de trabajos académicos

          • Vigilância dos norovirus GII: caracterização molecular de recombinantes e da variante emergente GII. P17 Kawazaki_2014 no Brasil (2018)
            Candidato: Juliana da Silva Ribeiro de Andrade
            Tipo Jurado: Tesis de Doctorado
            Gomez MM
            Pós-Graduação em Biologia Parasitária / Sector Extranjero/Internacional/Otros / Institución Extranjera / Instituto Oswaldo Cruz, Fundação Oswaldo Cruz / Brasil
            País: Brasil
            Idioma: Portugués
            Palabras Clave: norovirus recombinante brasil epidemiologia caracterizacao molecular
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular / Virologia
            Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución / Genómica
          • Avaliação do conhecimento de alunos da educação básica sobre hepatite C. (2017)
            Candidato: Tairine Monteiro de Barros
            Tipo Jurado: Iniciación científica
            Gomez MM
            Sector Extranjero/Internacional/Otros / Institución Extranjera / Universidade Estadual do Norte Fluminense Darcy Ribeiro / Brasil
            País: Brasil
            Idioma: Portugués
            Palabras Clave: HCV educacao basica
            Areas de conocimiento:
            Ciencias Médicas y de la Salud / Ciencias de la Salud / Ciencias Biomédicas Sociales
          • Etiologia viral nas gastrenterites agudas em Salvador, Bahia: os astrovírus. (2010)
            Candidato: Patrícia Duque Estrada
            Tipo Jurado: Iniciación científica
            Gomez MM
            Sector Extranjero/Internacional/Otros / Institución Extranjera / Universidad Federal de Río de Janeiro / Brasil
            País: Brasil
            Idioma: Portugués
            Areas de conocimiento:
            Ciencias Naturales y Exactas / Ciencias Biológicas / Ciencias Biológicas / Virologia
      • Información adicional

        Proyectos de investigación - participante:


        Proyecto: Genetic variability of Hepatitis C virus in Uruguay: Impact of new variants in the virus and disease understanding.

        Financiamiento: Consejo Nacional de Innovación, Ciencia y Tecnología (CONICYT).

        Período: 2003 - 2007.

        Coordinador: Dr. Juan Cristina Gheraldi.

         

        Proyecto: Genetic variability of Hepatitis C virus in Latin American Region. CRP.LA/URU03-02.

        Financiamiento: International Centre for Genetic Engineering and Biotechnology (ICGEB) / Pan American Health Organization (PAHO) / Latina American Network of Biological Sciences (RELAB).

        Período: 2004 - 2008.

        Coordinador: Dr. Juan Cristina Gheraldi.

         

        Proyecto: Implementation of a Quality Assurance and Control Network for the Molecular Diagnosis of diseases transmitted by insects. ARCAL LXXIII (RLA/6/050).

        Financiamiento: International Atomic Energy Agency (IAEA).

        Período: 2005 - 2007.

        Coordinador: Dr. Juan Cristina Gheraldi.

         

        Proyecto: Genes estruturais e não-estruturais de rotavírus A circulantes na Amazônia e no Sudeste brasileiro: genealogia, transmissão entre-espécies e impacto no programa nacional de imunização anti-rotavírus. 474274/2008-0.

        Financiamiento: CNPq.

        Período: 2008 - 2010.

        Coordinador: Dr. José Paulo Gagliardi Leite.

         

        Proyecto: Genes estruturais e não-estruturais de rotavírus A: transmissão inter-espécies e impacto no programa nacional de imunização anti-rotavírus. 403550/2008-4.

        Financiamiento: CNPq.

        Período: 2008 - 2010.

        Coordinador: Dr. José Paulo Gagliardi Leite.

         

        Proyecto: Variabilidade Genética e evolução molecular dos Rotavírus: seu impacto na doença e no desenvolvimento de novas vacinas. 006/08.

        Financiamiento: CAPES, Programa de Cooperação Internacional Capes/Udelar.

        Período: 2009 - 2011.

        Coordinador: Dr. José Paulo Gagliardi Leite.

         

        Proyecto: Epidemiologia molecular e monitoramento ambiental das gastrenterites de etiologia viral. PPCP 023/2011.

        Financiamiento: CAPES, Programa Capes PPCP-Mercosul.

        Período: 2012 - 2015.

        Coordinador: Dr. José Paulo Gagliardi Leite.

         

        Proyecto: Rotavirus da Espe?cie A: epidemiologia molecular, mecanismos evolutivos e impacto no programa nacional de imunizac?a?o anti-rotavirus A. 482119/2012-8.

        Financiamiento: CNPq.

        Inicio: 2012 - 2015.

        Coordinador: Dr. José Paulo Gagliardi Leite.

         

        Proyecto: Análise do genótipo emergente G12 de rotavírus no Brasil e desenvolvimento de metodologia molecular para caracterização da constelação gênica dos RVA.

        Financiamiento: FAPERJ.

        Inicio: 18/12/2015 / Duración: 48 meses.

        Coordinador: Dr. Eduardo de Mello Volotão.

         

        Proyecto: Estudos de marcadores de virulência e atenuação do vírus Zika e de sua infecção em modelos celulares e animais: perspectivas no estabelecimento de protótipos vacinais e no conhecimento da patogenia viral.

        Financiamiento: CAPES.

        Inicio: 24/10/2016. Duración: 24 meses.

        Coordinador: Dra. Myrna Cristina Bonaldo.


        Proyecto: Caracterización biológica y molecular del virus Y de la papa en cultivares de papa (Solanum tuberosum) en Uruguay y diseño de herramientas de diagnóstico molecular para detección y caracterización del virus.

        Financiamiento: Instituto de Investigaciones Biológicas Clemente Estable ? IIBCE.

        Inicio: 01/01/2019 / Duración: 24 meses.

        Coordinador: Dra. Inés Ponce de León / M.Sc.Mercedes Peyrou.


        Revisor Ad Hoc

         

        -          MEEGID - Infection, Genetics and Evolution

        -          Virus Research

        -          BMC Infectious Diseases

        -          Revista Pan-Amazônica de Saúde

        -          Jornal de Pediatria

        -          Virus Disease

        -          Epidemiology and Infection

        -   PLOS ONE


      • Indicadores de producción

        Producción bibliográfica

        59
        Artículos publicados en revistas científicas
        29
        Completo 29
        Trabajos en eventos
        29
        Libros y Capítulos
        1
        Capítulos de libro publicado 1
        Otros tipos
        2

        Producción técnica

        2

        Formación RRHH

        3
        Tutorías/Orientaciones/Supervisiones concluidas
        3
        Otras tutorías/orientaciones 3