Datos Personales

• Identidad

  Nombre en citaciones bibliográficas: VENZAL, J.M.
  Documento: Cédula de identidad uruguay - 19126770 , Pasaporte/Documento extranjero - D240062
  Sexo: Masculino
  País de pasaporte: Uruguay
  Fecha de nacimiento: 05/12/1974
  Lugar de nacimiento: Uruguay / Salto / Salto
  País de Nacionalidad: Uruguay

• Dirección personal

  Dirección: Jose Pedro Varela 851 / 50000
  País: Uruguay / Salto / Salto
  Teléfono: (598) 47328167
  Correo electrónico: dpvuru@hotmail.com

Datos Generales

• Institución principal

  Universidad de la República/ Centro Universitario Regional Litoral Norte / Departamento de Ciencias Biológicas, Laboratorio de Vectores y enfermedades transmitidas / Uruguay

• Dirección institucional

  Institución: Universidad de la República / Centro Universitario Regional Litoral Norte / Sector Educación Superior/Público
  / Laboratorio de Vectores y Enfermedades Transmitidas
  Dirección: Rivera 1350 / 50000
  País: Uruguay / Salto / Salto
  Teléfono: (598) 47334816 / 3950
  Correo electrónico/Sitio Web: dpvuru@hotmail.com http://www.unorte.edu.uy/
  

Formación

• Formación académica

  • Concluida

    • Doctorado

      • 1009-1-Patología Animal: Sanidad Animal (2005 - 2008)

        Universidad de Zaragoza , España
        Título de la disertación/tesis/defensa: Estudios sobre garrapatas y enfermedades transmitidas en Uruguay: Aspectos epidemiológicos de la rickettsiosis humana en un área endémica. El grupo Ornithodoros (Alectorobius) talaje: sistemática, descripción de una nueva especie y efectos patógenos.
        Tutor/es: Agustín Estrada Peña
        Obtención del título: 2008
        Financiación:
        Banco Santander Central Hispano , España
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

    • Grado

      • Doctor en Medicina y Tecnología Veterinaria* (1994 - 2002)

        Universidad de la República - Facultad de Veterinaria , Uruguay
        Título de la disertación/tesis/defensa:
        Obtención del título: 2002
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

• Formación complementaria

  • Concluida

    • Cursos de corta duración

      • Introducción a la Ética de la Investigación (03/2023 - 03/2023)

        Sector Educación Superior/Público / Universidad de la República / Universidad de la República - Unidad Central de Educación Permanente , Uruguay
        8 horas

      • Curso de Tiempo y Clima en la región de Salto Grande (11/2022 - 11/2022)

        Sector Extranjero/Internacional/Organismos internacionales / Organismos Internacionales / Comisión Técnica Mixta de Salto Grande / Polo Binacional Educativo Científico Tecnológico y Productivo , Uruguay
        2 horas

      • Manejo y uso de Animales No Tradicionales en Investigación ? 2021 (07/2021 - 09/2021)

        Sector Educación Superior/Público / Universidad de la República / Centro Universitario Regional del Este , Uruguay
        90 horas

      • Curso-Taller: Enseñar en EVA-Fvet Virtual (PROGRAMA FORMACION DOCENTE). (02/2021 - 02/2021)

        Sector Educación Superior/Público / Universidad de la República / Facultad de Veterinaria / Departamento Educación Veterinaria , Uruguay
        20 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • HABLANDO DE ÁCAROS: Tópicos de actualización en Acarología Agrícola, Médico Veterinaria, y Biodiversidad. (05/2020 - 07/2020)

        Sector Extranjero/Internacional/Otros / Sociedad Latinoamericana de Acarología / Sociedad Latinoamericana de Acarología. , Argentina
        50 horas
        Palabras Clave: Modalidad del curso: Virtual (plataforma de Google Meet (Hangout Meet)).
        Areas de conocimiento:
        Ciencias Agrícolas / Agricultura, Silvicultura y Pesca / Agronomía, reproducción y protección de plantas /
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Curso Técnicas Helmintológicas (01/2009 - 01/2009)

        Sector Extranjero/Internacional/Otros / Universidad Nacional de La Plata, V Congreso Argentino de Parasitología , Argentina
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología

      • Salud, bienestar y producción de animales de laboratorio" (CHEA, CSIC) (01/2008 - 01/2008)

        Sector Educación Superior/Público / Universidad de la República / Facultad de Veterinaria , Uruguay
        45 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Taller en Biodiversidad y Taxonomía: Presente y Futuro en el Uruguay (01/2004 - 01/2004)

        Sector Educación Superior/Público / Universidad de la República / Facultad de Ciencias , Uruguay
        30 horas
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • Curso Internacional sobre Progresos en el Diagnóstico de las Parasitosis de los Animales de Producción (01/2004 - 01/2004)

        Sector Extranjero/Internacional/Otros / Escola de Medicina Veterinaria de la Universidad Federal de Bahia , Brasil
        264 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Parásitos de micromamíferos: Técnicas de estudio y diagnóstico de los principales grupos. Aspectos de la biología y ecología (01/2004 - 01/2004)

        Sector Extranjero/Internacional/Otros / Universidad Nacional de Tucumán , Argentina
        80 horas
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología

      • Actualización de Enfermedades Parasitarias de Importancia Económica (01/2002 - 01/2002)

        Sector Educación Superior/Público / Universidad de la República / Facultad de Veterinaria , Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Aves de la selva y el monte (01/2001 - 01/2001)

        Sector Organizaciones Privadas sin Fines de Lucro/Organizaciones No Gubernamentales / Organizaciones Sin Fines de Lucro / Vida Silvestre Uruguay , Uruguay
        12 horas
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • Mamíferos del Uruguay (01/2000 - 01/2000)

        Sector Organizaciones Privadas sin Fines de Lucro/Organizaciones No Gubernamentales / Organizaciones Sin Fines de Lucro / Vida Silvestre Uruguay , Uruguay
        15 horas
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • Cria de Conejos (01/2000 - 01/2000)

        Sector Educación Superior/Público / Universidad de la República / Facultad de Veterinaria , Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Producción Animal y Lechería / Cría Animal / Cunicultura

      • Módulo Básico Capacitación e Identificación Entomólogica para Aedes aegypti -Triatominae OPS-OMS (01/1999 - 01/1999)

        Sector Educación Superior/Público / Universidad de la República / Facultad de Medicina , Uruguay
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

      • Curso Básico de Nutrición Canina y Felina (01/1998 - 01/1998)

        Sector Educación Superior/Público / Universidad de la República / Facultad de Veterinaria , Uruguay
        8 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Aves del Uruguay - Introducción a la Ornitología (01/1998 - 01/1998)

        Sector Extranjero/Internacional/Otros / Otra institución nacional / Aves Uruguay , Uruguay
        10 horas
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

    • Participación en eventos

      • Participante da atividade Conferencia - Prospecção e uso de ácaros predadores no controle de pragas e parasitos (2024)

        Tipo: Otro
        Institución organizadora: Sociedad Latinoamericana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Participante da palestra: Encontro entre a Acarología e a Agroecologia (2024)

        Tipo: Otro
        Institución organizadora: Sociedad Latinoamericana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Participante da palestra: Ácaros forenses II Parte (2024)

        Tipo: Otro
        Institución organizadora: Sociedad Latinoamericana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Participante da palestra: Generalidades de los ácaros acuáticos (Acari: Hydrachnidia) (2024)

        Tipo: Otro
        Institución organizadora: Sociedad Latinoamericana de Acarologia, Brasil
        Alcance geográfico: Regional

      • 1° JORNADAS DE ACTUALIZACIÓN. AVANCES Y DESAFÍOS EN EL DIAGNÓSTICO Y LA VIGILANCIA DE ZOONOSIS BACTERIANAS TRANSMITIDAS POR VECTORES. UNA PERSPECTIVA INTEGRADA (2024)

        Tipo: Otro
        Institución organizadora: Instituto Nacional de Enfermedades Infecciosas "Dr. Carlos G. Malbrán" / ANLIS MALBRÁN.. 17 de octubre de 2024 - 8:30 a 16:30 horas., Argentina
        Alcance geográfico: Nacional

      • TALLER SOBRE RAOIELLA INDICA. A 20 años de su llegada al Continente Americano (2024)

        Tipo: Taller
        Institución organizadora: Sociedad Latinoamericana de Acarología. Carga horaria: 15 horas., Brasil
        Alcance geográfico: Regional

      • Palestra Febre Maculosa Brasileira, realizada el 20/07/2023, Ciclo de conferencias de la Sociedad Latinoamericana de Acarologia - 2023. (2023)

        Tipo: Otro
        Institución organizadora: Sociedad Latinoamericana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Palestra Controle Biológico de Ácaros de Importância Veterinária, realizada el 29/03/2023, Ciclo de conferencias de la Sociedad Latinoamericana de Acarologia - 2023 (2023)

        Tipo: Otro
        Institución organizadora: Sociedad Latinoamericana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Palestra Manejo de ácaros em ambientes tropicais, realizada el 24/05/2023, Ciclo de conferencias de la Sociedad Latinoamericana de Acarologia - 2023 (2023)

        Tipo: Otro
        Institución organizadora: Sociedad Latinoamericana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Jornadas "NFLUENZA AVIAR en URUGUAY 2023 - ASPECTOS EPIDEMIOLÓGICOS en FAUNA SILVESTRE (2023)

        Tipo: Otro
        Institución organizadora: Academia Nacional de Veterinaria, Uruguay
        Alcance geográfico: Nacional

      • Asistencia al VII Congreso Uruguayo de Zoología . Damaso Antonio Larrañaga (2023)

        Tipo: Congreso
        Institución organizadora: Sociedad Zoológica del Uruguay. 3 al 8 de diciembre de 2023. Montevideo, Uruguay., Uruguay
        Alcance geográfico: Nacional

      • Participante da palestra: Investigaciones asociadas al estudio de ácaros fitófagos en el centro del continente Americano (2023)

        Tipo: Otro
        Institución organizadora: Sociedad Latinoamericana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Participante da palestra: Interacciones tróficas en el suelo: un componente clave para control biológico por conservación (2023)

        Tipo: Otro
        Institución organizadora: Sociedad Latinoamericana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Participante da palestra: Novedades de los ácaros fitófagos en las Américas: un tremendo reto (2023)

        Tipo: Otro
        Institución organizadora: Sociedad Latinoamericana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Participante da palestra: Los ácaros caecúlidos fósiles: sus aportes a la sistemática y la filogenia de la familia (2023)

        Tipo: Otro
        Institución organizadora: Sociedad Latinoamericana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Palestra Controle biológico de ácaros fitófagos com ácaros predadores realizada em 24/08/2022, Ciclo de conferências da Sociedade Latino-americana de Acarologia ? 2022 (2022)

        Tipo: Otro
        Institución organizadora: Sociedade Latino-americana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Palestra Distribución de los ácaros edaficos en la estepa patagónica, realizada em 26/05/2022. Ciclo de conferências da Sociedade Latino-americana de Acarologia - 2022 (2022)

        Tipo: Otro
        Institución organizadora: Sociedade Latino-americana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Palestra Los ácaros predadores edáficos en el Brasil, realizada em 23/06/2022, Ciclo de conferências da Sociedade Latino-americana de Acarologia - 2022 (2022)

        Tipo: Otro
        Institución organizadora: Sociedade Latino-americana de Acarologia, Brasil

      • Palestra Ácaros en ecosistemas antropizados de México em 29/07/2022, Ciclo de conferências da Sociedade Latino-americana de Acarologia ? 2022 (2022)

        Tipo: Otro
        Institución organizadora: Sociedade Latino-americana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Palestra Filogenia dos ácaros Acariformes: o que nos trouxe até aqui e perspectivas, realizada em 18/11/2022, Ciclo de conferências da Sociedade Latino-americana de Acarologia ? 2022 (2022)

        Tipo: Otro
        Institución organizadora: Sociedade Latino-americana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Palestra Las especies crípticas y la subestimación de la diversidad en ácaros parásitos: estudio de caso, realizada em 15/12/2022, Ciclo de conferências da Sociedade Latino-americana de Acarologia ? 2022 (2022)

        Tipo: Otro
        Institución organizadora: Sociedade Latino-americana de Acarologia, Brasil
        Alcance geográfico: Regional

      • Palestra Ácaros acuáticos. Ciclo de conferências mensais e virtuais da Sociedade Latinoamericana de Acarologia - 2021. Realizada em 29/01/2021. (2021)

        Tipo: Otro
        Institución organizadora: Sociedade Latinoamericana de Acarologia., Brasil

      • VII Simpósio Brasileiro de Acarologia (VII SIBAC). Acarologia em tempos de conectividade (2021)

        Tipo: Simposio
        Alcance geográfico: Nacional

      • Palestra Ácaros forenses. Ciclo de conferências mensais e virtuais da Sociedade Latinoamericana de Acarologia - 2021. Realizada em 27/02/2021. (2021)

        Tipo: Otro
        Institución organizadora: Sociedade Latinoamericana de Acarologia., Brasil

      • Palestra Estado do conhecimento sobre tarsonemideos e tenuipalpideos na América Latina: desafios e perspectivas. Ciclo de conferências mensais e virtuais da Sociedade Latinoamericana de Acarologia - 2021. Realizada em 25/03/2021. (2021)

        Tipo: Otro
        Institución organizadora: Sociedade Latinoamericana de Acarologia., Brasil

      • Palestra Varroosis en abejas: situación en Nicaragua y Argentina. Ciclo de conferências mensais e virtuais da Sociedade Latinoamericana de Acarologia - 2021. Realizada em 29/04/2021. (2021)

        Tipo: Otro
        Institución organizadora: Sociedade Latinoamericana de Acarologia., Brasil

      • Palestra Ácaros oribátidos, de la Amazonia a las Pampas. Ciclo de conferências mensais e virtuais da Sociedade Latinoamericana de Acarologia - 2021. Realizada em 27/05/2021. (2021)

        Tipo: Otro
        Institución organizadora: Sociedade Latinoamericana de Acarologia, Brasil

      • Palestra Control microbiano de ácaros fitófagos y de garrapatas- estado del arte, desafíos y perspectivas para Latino América. Ciclo de conferências mensais e virtuais da Sociedade Latinoamericana de Acarologia - 2021. Realizada em 24/06/2021. (2021)

        Tipo: Otro
        Institución organizadora: Sociedade Latinoamericana de Acarologia, Brasil

      • Palestra Raoiella un genero multifacetico!. Ciclo de conferências mensais e virtuais da Sociedade Latinoamericana de Acarologia - 2021. Realizada em 28/10/2021. (2021)

        Tipo: Otro
        Institución organizadora: Sociedade Latinoamericana de Acarologia, Brasil

      • Palestra Ácaros ectosimbiontes de murciélagos. Ciclo de conferências mensais e virtuais da Sociedade Latinoamericana de Acarologia - 2021. Realizada em 25/11/2021. (2021)

        Tipo: Otro
        Institución organizadora: Sociedade Latinoamericana de Acarologia, Brasil

      • 2° Jornada Internacional de Diagnóstico de Laboratorio de las Echinococcosis del Cono Sur: Herramientas innovadoras aplicables desde otros helmintos, 30 de noviembre del 2021. San Luis, Argentina. (2021)

        Tipo: Otro
        Institución organizadora: ICT Dr. Cesár Milstein - CONICET, Mundo Sano, Min. de Salud del Gob. de la Prov. de San Juan y la Fac. Cs. Veterinarias de la UCCuyo sede San Luis, San Luis, Argentina., Argentina

      • VI CONGRESO URUGUAYO DE ZOOLOGÍA ?60 Aniversario de la SZU? y III ENCUENTRO INTERNACIONAL DE ECOLOGÍA Y CONSERVACIÓN. (2021)

        Tipo: Congreso
        Institución organizadora: Sociedad Zoológica del Uruguay., Uruguay
        Alcance geográfico: Nacional

      • Palestra Ácaros plumícolas: la fauna que pulula en la jungla de las plumas Ácaros plumícolas: la fauna que pulula en la jungla de las plumas. Ciclo de conferências mensais e virtuais da Sociedade Latinoamericana de Acarologia - 2021. Realizada em 30/09/20 (2021)

        Tipo: Otro
        Institución organizadora: Sociedade Latinoamericana de Acarologia., Brasil

      • VII Congreso Latinoamericano de Enfermedades Rickettsiales, 6 al 8 de noviembre de 2019. (2019)

        Tipo: Congreso
        Institución organizadora: Universidad Autónoma De Baja California, Mexicali, México

      • XI Jornadas Técnicas Veterinarias, 1 al 2 de octubre de 2019, (2019)

        Tipo: Otro
        Institución organizadora: Facultad de Veterinaria, Universidad de la República, Montevideo,, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Jornada de Infecciones priónicas en el Uruguay (vaca loca), 5 de octubre de 2019. (2019)

        Tipo: Otro
        Institución organizadora: Centro Médico de Salto, Uruguay
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Ciencias de la Salud /

      • V Congreso Uruguayo de Zoología.2018. 9 al 14 de diciembre de 2018, Facultad de Ciencias, Montevideo. (2018)

        Tipo: Congreso
        Institución organizadora: Sociedad Zoológica del Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • II Congreso Internacional de Zoonosis y IX Congreso Argentino de Zoonosis ?Alimentos y Zoonosis: Desafíos del Siglo XXI? (2018)

        Tipo: Congreso
        Institución organizadora: Asociación Argentina de Zoonosis (AAZ), Argentina
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas / Zoonosis

      • 2do. Encuentro de Investigadores del Noreste, "Dr. Guillermo Pérez Suárez" (2018)

        Tipo: Encuentro
        Institución organizadora: Centro Universitario de Rivera, Universidad de la República, Rivera, Uruguay, Uruguay

      • IV Congreso Uruguayo de Zoología, 4 al 9 de diciembre de 2016 (2016)

        Tipo: Congreso
        Institución organizadora: Sociedad Zoológica del Uruguay; Centro Universitario Regional del Este, Uruguay
        Palabras Clave: Uruguay Congreso Zoología
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • 9as Jornadas Técnicas Veterinarias (2015)

        Tipo: Otro
        Institución organizadora: Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay, Uruguay
        Palabras Clave: Jornadas Veterinaria
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Asistencia al VII Congreso Argentino de Parasitología, 1 al 5 de noviembre de 2015 (2015)

        Tipo: Congreso
        Institución organizadora: Asociación Parasitológica Argentina, San Carlos de Bariloche, Río Negro, Argentina, Uruguay
        Palabras Clave: Parasitología
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Taller de capacitación para trabajo de campo en proyecto de vigilancia y control de la leishmaniasis canina en Uruguay-IDRC. Salto, Uruguay. (2015)

        Tipo: Taller
        Institución organizadora: Ministerio de Salud Pública, Uruguay
        Palabras Clave: Uruguay Salto Control Leishmaniasis vigilancia
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

      • V Simpósio Brasileiro de Acarologia (2015)

        Tipo: Simposio
        Institución organizadora: UNESP - Universidade Paulista, Brasil
        Palabras Clave: Acarología
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

      • XVIII Congresso Brasileiro de Parasitologia Veterinária, 21 a 24 de outubro de 2014, Gramado (2014)

        Tipo: Congreso
        Institución organizadora: Colegio Brasileiro de Parasitologia Veterinária, Brasil
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Jornada de Conferencias AUCyTAL, 13 de Setiembre de 2014, Aula MAGNA - REGIONAL NORTE, Salto (2014)

        Tipo: Otro
        Institución organizadora: Asociación Uruguaya de Ciencia y Tecnología de los Animales de Laboratorio y el CEUA de Regional Norte, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • III Simpósio Estadual de Doenças Transmitidas por Carrapatos, Campinas (2013)

        Tipo: Simposio
        Institución organizadora: Superintendência de Controle de Endemias (SUCEN), Brasil
        Palabras Clave: Garrapatas Enfermedades transmitidas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

      • IV Simpósio Brasileiro de Acarologia (2013)

        Tipo: Simposio
        Palabras Clave: Acarología
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • IV Congreso Latinoamericano de Enfermedades Rickettsiales (2013)

        Tipo: Congreso
        Institución organizadora: Universidad de Costa Rica, San José, Costa Rica, Costa Rica
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas / Rickettsiosis

      • Segundo Congreso Uruguayo de Zoología (2012)

        Tipo: Congreso
        Institución organizadora: Sociedad Zoológica del Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • VII Jornadas Técnicas Veterinarias (2011)

        Tipo: Congreso
        Institución organizadora: Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Primer Congreso Uruguayo de Zoología, X Jornadas de Zoología del Uruguay (2010)

        Tipo: Congreso
        Institución organizadora: Sociedad Zoológica del Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • Taller Internacional “Presente y futuro de las Investigaciones sobre Sistemática y Ecología de garrapatas Neotropicales” (2010)

        Tipo: Taller
        Institución organizadora: Instituto Nacional de Tecnología Agropecuaria , Rafaela, Santa Fe, Argentina, Argentina
        Palabras Clave: Garrapatas ecología Sistemática
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Seminario para Autores (2009)

        Tipo: Seminario
        Institución organizadora: Elsevier y ANII, Uruguay

      • II Taller de enseñanza de la Parasitología (2009)

        Tipo: Taller
        Institución organizadora: Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Esperanza, Santa Fe, Argentina, Argentina
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Jornada Teórica-Práctica sobre Leishmaniasis. Entre Ríos, Concordia (2009)

        Tipo: Taller
        Institución organizadora: Centro Departamental Concordia (CMVER). Colegio de Médicos Veterinarios de Entre Ríos, Argentina
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • V Congreso Argentino de Parasitología, La Plata, Buenos Aires (2009)

        Tipo: Congreso
        Institución organizadora: Asociación Parasitológica Argentina, Argentina
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología

      • IX Jornadas de Zoología del Uruguay (2008)

        Tipo: Congreso
        Institución organizadora: Sociedad Zoológica del Uruguay, Facultad de Ciencias, Montevideo, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • V Jornadas Técnicas de la Facultad de Veterinaria (2007)

        Tipo: Congreso
        Institución organizadora: Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Taller Regional Aplicación del Control Integrado de Parásitos (CIP) a la garrapata Boophilus microplus en Uruguay (2007)

        Tipo: Taller
        Institución organizadora: DILAVE “Miguel C. Rubino”, Montevideo, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • VIII Jornadas de Zoología del Uruguay (2005)

        Tipo: Congreso
        Institución organizadora: Sociedad Zoológica del Uruguay, Facultad de Ciencias, Montevideo, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • 4th International Conference on Rickettsiae and Rickettsial Diseases, La Rioja, Logroño (2005)

        Tipo: Congreso
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /

      • XIII Congresso Brasileiro de Parasitologia Veterinaria e I Simpósio Latino-Americano de Rickettsioses. Ouro Preto, Minas Gerais (2004)

        Tipo: Congreso
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • VII Jornadas de Zoología del Uruguay (2003)

        Tipo: Congreso
        Institución organizadora: Sociedad Zoológica del Uruguay, Facultad de Ciencias, Montevideo, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • Reunión de Especialistas en Parasitología Veterinaria de Argentina, Brasil, Chile, Paraguay y Uruguay. 11º Encuentro de Veterinarios Endoparasitólogos Rioplatenses, Tandil, Buenos Aires (2002)

        Tipo: Encuentro
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • 1er. Encuentro Anual sobre Experiencias de Aula en la Facultad de Veterinaria (2002)

        Tipo: Encuentro
        Institución organizadora: Facultad de Veterinaria, Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • VII Congreso Nacional de Veterinaria - III Congreso Nacional de Veterinarios Especialistas en Pequeños Animales - III Congreso Iberoamericano de Veterinarios Especializados en Equinos (2001)

        Tipo: Congreso
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • IX Jornadas de Jóvenes Investigadores de la A.U.G.M. “Ciencia y Tecnología para la Integración Regional” (2001)

        Tipo: Congreso
        Institución organizadora: Universidad Nacional de Rosario, Rosario, Argentina
        Palabras Clave: Ixodes Garrapatas Enfermedades transmitidas Enfermedad de Lyme
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Perspectivas de las Investigaciones Parasitológicas en la Mastozoología Neotropical (2001)

        Tipo: Taller
        Institución organizadora: XVI Jornadas Argentinas de Mastozoología, Mendoza, Argentina, Argentina
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Otras Ciencias Naturales / Otras Ciencias Naturales /

      • Seminario Regional de Rabia (2001)

        Tipo: Seminario
        Institución organizadora: Facultad de Veterinaria, Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Biología y Ecología de los Pinnipedios (2001)

        Tipo: Otro
        Institución organizadora: SUVEAS (Sociedad Uruguaya de Veterinarios Especialistas en animales Silvestres), Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • Conferencia sobre Pediatría Canina y Felina dictada por el Dr. Antonio Prats Esteve (España) (2001)

        Tipo: Otro
        Institución organizadora: Departamento de Pequeños Animales, Facultad de Veterinaria, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • VI Jornadas de Zoología del Uruguay (2001)

        Tipo: Congreso
        Institución organizadora: Sociedad Zoológica del Uruguay, Facultad de Ciencias, Montevideo, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • IX Congresso Brasileiro de Ornitologia “Ornitología sem fronteiras” - VII Encontro Nacional de Anilhadores de Aves - II Encontro de Ornitólogos do MERCOSUL, Curitiba, Paraná, Brasil (2001)

        Tipo: Congreso
        Institución organizadora: Sociedade Brasileria de Ornitologia, Brasil
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • XXI Congreso Mundial de Buiatría - XXVIII Jornadas Uruguayas de Buiatría (2000)

        Tipo: Congreso
        Institución organizadora: World Buiatric Association, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Actualización en Mosca de los Cuernos (2000)

        Tipo: Taller
        Institución organizadora: Facultad de Veterinaria, Universidad de la República y Plan Agropecuario, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • IV Congreso Nacional de Fauna (2000)

        Tipo: Congreso
        Institución organizadora: Universidad Nacional de Río Cuarto, Córdoba, Argentina, Argentina
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • III Congreso Argentino de Parasitología, Mar del Plata, Argentina. (2000)

        Tipo: Congreso
        Institución organizadora: Sociedad Argentina de Parasitología, Argentina
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Simposio internacional sobre enfermedades prionicas en el animal y en el hombre (2000)

        Tipo: Simposio
        Institución organizadora: Facultad de Ciencias, Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /

      • Jornadas de Antibióticos en Leche (2000)

        Tipo: Congreso
        Institución organizadora: Hotel Nirvana, Nueva Helvecia, Colonia, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Migración de aves (2000)

        Tipo: Otro
        Institución organizadora: SUVEAS (Sociedad Uruguaya de Veterinarios Especialistas en animales Silvestres), Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • Seminario de actualización en patología de Pequeños Rumiantes (1999)

        Tipo: Seminario
        Institución organizadora: Facultad de Veterinaria, Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • V Jornadas de Zoología del Uruguay y I Encuentro de Entomología Médica y Veterinaria (1999)

        Tipo: Congreso
        Institución organizadora: Facultad de Ciencias, Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • Campañas sanitarias en ectoparásitos (1999)

        Tipo: Taller
        Institución organizadora: Departamento de Parasitología Veterinaria, Facultad de Veterinaria, Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Endoparásitos en cría y recría de ganado lechero (1999)

        Tipo: Taller
        Institución organizadora: Departamento de Parasitología Veterinaria, Facultad de Veterinaria, Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Control antihelmíntico en rumiantes (1998)

        Tipo: Seminario
        Institución organizadora: Departamento de Parasitología Veterinaria, Facultad de Veterinaria, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Jornadas de Vacunas en Veterinaria, realizadas por el Área de Inmunología (1998)

        Tipo: Seminario
        Institución organizadora: Departamento de Ciencias Microbiológicas, Facultad de Veterinaria, Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Jornadas de Discusión sobre alternativas y metodologías de las pasantías a desarrollarse en la Estación de cría del Cerro Pan de Azúcar, Maldonado, Uruguay. (1998)

        Tipo: Encuentro
        Institución organizadora: Facultad de Veterinaria, Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Otras Ciencias Agrícolas / Otras Ciencias Agrícolas /

      • II Taller Nacional de Conservación del Venado de Campo (1996)

        Tipo: Taller
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • IV Jornadas de Zoología del Uruguay (1996)

        Tipo: Congreso
        Institución organizadora: Sociedad Zoológica del Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /

      • Primeras Jornadas Científicas sobre el Medio Ambiente (1995)

        Tipo: Congreso
        Institución organizadora: Asociación de Universidades Grupo Montevideo, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Conservación de la Biodiversidad /

      • 3eras. Jornadas Técnicas de la Facultad de Veterinaria (1994)

        Tipo: Congreso
        Institución organizadora: Facultad de Veterinaria, Montevideo, Uruguay, Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Segundo Taller de ONGs Ambientalistas (1994)

        Tipo: Taller
        Institución organizadora: Cuerpo de Paz de los EEUU, Colonia Suiza, Colonia,, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Conservación de la Biodiversidad /

      • Taller Regional de Educación Ambiental (1993)

        Tipo: Taller
        Institución organizadora: Comisión Honoraria Asesora en Materia de Fauna y Flora Silvestre, Salto, Uruguay
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Conservación de la Biodiversidad /

Idiomas

• Francés

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• Inglés

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• Portugués

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Areas de actuación

• Ciencias Agrícolas

  Ciencias Veterinarias /Ciencias Veterinarias /Parasitología

• Ciencias Médicas y de la Salud

  Ciencias de la Salud /Parasitología /Ectoparásitos

• Ciencias Naturales y Exactas

  Ciencias Biológicas /Zoología, Ornitología, Entomología, Etología

Actuación profesional

• Sector Educación Superior/Público - Universidad de la República - Uruguay

  Facultad de Veterinaria

  • Vínculos con la Institución

    • Colaborador (12/2024 - a la fecha)

      Integrante dentro de la Categoría A1 del Núcleo de Investigadores del Programa NIP: 2 horas semanales
      Programa de Posgrado de la Facultad de Veterinaria (PPFV).

    • Colaborador (06/2024 - a la fecha)

      Integrante de la Subcomisión Salud Animal del Programa de Posgrado de la Facultad de Veterinaria (PP 2 horas semanales
      Esta integración está de acuerdo al artículo 16 de la nueva ordenanza de posgrados de la Universidad de la República.

    • Otro (11/2014 - a la fecha)

      Docente del Laboratorio de Vectores y Enfermedades Transmitidas como Unidad Asociada a la Facultad d 2 horas semanales
      Escalafón: Docente

    • Colaborador (02/2013 - 11/2024)

      Confirmación como docente de Maestrías. Programa de Posgrado de Facultad de Veterinaria, Universidad 2 horas semanales
      Exp. Nº 111460-000122-12
      Escalafón: No Docente

    • Funcionario/Empleado (11/2013 - 06/2015)Trabajo relevante

      Profesor Adjunto 20 horas semanales
      Escalafón: Docente
      Grado: Grado 3
      Cargo: Efectivo

    • Funcionario/Empleado (02/2008 - 11/2013)

      Profesor Adjunto 40 horas semanales
      Concurso de Méritos y Prueba, Proyecto de Investigación y Plan de Desarrollo Académico del Área
      Escalafón: Docente
      Grado: Grado 3
      Cargo: Efectivo

    • Colaborador (01/2009 - 01/2013)

      Docente Honorario de Maestrías. Programa de Posgrado de Facultad de Veterinaria, Universidad de la R 2 horas semanales
      Exp. Nº 111460-000227-09
      Escalafón: No Docente

    • Funcionario/Empleado (08/2003 - 01/2008)

      Ayudante 40 horas semanales
      Escalafón: Docente
      Grado: Grado 1
      Cargo: Interino

    • Funcionario/Empleado (01/2000 - 08/2003)

      Ayudante 20 horas semanales
      Concurso de Méritos y Prueba
      Escalafón: Docente
      Grado: Grado 1
      Cargo: Interino

    • Colaborador (06/1999 - 12/1999)

      Colaborador honorario 20 horas semanales
      Colaborador honorario y ayudante de clases prácticas curriculares de Enfermedades Parasitarias de 1999, con activa participación en la clase práctica de reconocimiento de ixódidos. Departamento de Parasitología Veterinaria, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
      Escalafón: Docente
      Grado: Grado 1
      Cargo: Interino

    • Colaborador (01/1998 - 12/1999)

      Colaborador en proyectos e investigación 20 horas semanales
      Proyectos: “Estudios preliminares de los parásitos del Ñandú (Rhea americana intermedia) de cría comercial” (1998) y “Estudio de las especies y biología de ixódidos hallados en micromamíferos (Muridae y Didelphidae como Monodelphis dimidiata) de la fauna uruguaya” (1999). Departamento de Parasitología Veterinaria, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
      Escalafón: No Docente
      Cargo: Interino

    • Becario (09/1997 - 08/1998)

      Ayudante de investigaciónCIDEC 20 horas semanales
      Orientadora: Dra. Perla Alicia Cabrera. Plan de trabajo: “Estudio de los ectoparásitos de la fauna silvestre y su acción como reservorio de posibles especies patógenas para los animales domésticos”. Departamento de Parasitología Veterinaria, Facultad de Veterinaria, Montevideo, Uruguay.
      Escalafón: Docente
      Grado: Grado 1
      Cargo: Interino

  • Actividades

    • Líneas de investigación

      • Garrapatas y enfermedades transmitidas (01/2000 - 06/2015 )

        Sub-líneas: 1 - Sistemática de Argásidos e Ixódidos de la Región Neotropical. 2 - Enfermedades transmitidas por garrapatas a humanos, animales domésticos y de producción. 3 - Ecología de garrapatas Neotropicales. Esta es la principal línea de investigación, la cual desde el año 2001 ha generado numerosas publicaciones, muchas de ellas provenientes de resultados obtenidos en proyectos concursables. Si bien inicialmente se trabajó en sistemática de garrapatas en la descripción de nuevas especies utilizando técnicas clásicas, así como moleculares, más recientemente se han determinado una amplia variedad de microrganismos transmitidos por garrapatas de los cuales muchos de ellos son de importancia en la salud animal y pública, con carácter zoonótico. Además de trabajar con investigadores de diferentes Instituciones de Uruguay, la línea sigue manteniendo vínculos estrechos con investigadores extranjeros: INTA Rafaela (Argentina), Universidad de Sao Paulo (Brasil), Instituto Butantan (Brasil), Universidad de Concepción (Chile), Hospital de La Rioja, Logroño (España), Universidad de Zaragoza (España), Universidad Central del Ecuador (Ecuador), Universidad de San Marcos (Perú), entre otras. A modo de ejemplo se destaca la descripción de nuevas especies de garrapatas como: Ornithodoros rioplatensis (Uruguay), Amblyomma boeroi (Argentina), Argas keiransi (Chile) y Antricola guglielmonei (Brasil), totalizando más de 20 nuevas especies descriptas para Sudamérica. Entre los principales agentes patógenos determinados, el uso de técnicas moleculares como PCR permitió esclarecer el agente de la rickettsiosis humana en Uruguay, Rickettsia parkeri, transmitida por la garrapata Amblyomma triste en los departamentos del sur de Uruguay. También se caracterizó por primera vez la presencia en el hemisferio sur de dos genotipos de Borrelia burdorferi, relacionados con los agentes causantes de la enfermedad de Lyme en el hemisferio norte. Más recientemente se han detectado varias bacterias (Ehrlichia, Anaplasma) y protozoarios (Babesia, Rangelia, Hepatozoon, etc.) tanto en garrapatas como en tejidos de animales silvestres y domésticos.
        40 horas semanales
        Facultad de Veterinaria, Departamento de Parasitología Veterinaria , Coordinador o Responsable
        Equipo: CASTRO, O. , FÉLIX, M.L.
        Palabras clave: Ixodidae Argasidae Garrapatas Enfermedades transmitidas Rickettsiosis
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

      • Aspectos sanitarios de la fauna íctica de la región de Salto Grande. (11/2013 - 06/2015 )

        Un embalse con las dimensiones y características del de Salto Grande albergan una rica fauna ictícola, la cual es objeto de pesca artesanal para consumo, pesca recreativa y deportiva. A su vez, los peces dulceacuícolas suelen albergar una variada fauna parasitaria, la cual es muy poco conocida en la región. Menos conocido aún es el potencial impacto que estos parásitos pueden tener sobre la aptitud y sobrevivencia de las poblaciones de peces. Este último aspecto se potencia en el caso del embalse de Salto Grande, por tratarse de un ecosistema artificial relativamente reciente, en el que los peces y su fauna asociada aún pueden no encontrarse en un estado de equilibrio. Por otra parte, las zoonosis vinculadas al consumo de pescado han aumentado su incidencia en años recientes, en particular, por la popularización de comidas en base a pescado crudo o poco cocido. Los carnívoros domésticos que se alimenten en parte con, o que tengan acceso a, vísceras de pescados también son susceptibles de albergar diversos parásitos transmitidos por los mismos. Además, la evidencia acerca de la presencia de biocidas organoclorados en peces del embalse de Salto Grande plantea la interrogante acerca del impacto de éstos sobre la salud de los peces. A priori, si la acumulación de biocidas en los tejidos de un pez afecta negativamente su fisiología y el correcto funcionamiento del sistema inmune, sería de esperar encontrar una correlación positiva entre la concentración de biocidas y la carga y/o la diversidad parasitaria albergada por el pez. Se realizaron dos subproyectos: uno es sobre las potenciales zoonosis parasitarias en peces de interés comercial y deportivo, donde se estableció como prioritario el estudio de las siguientes especies: Dorado (Salminus brasiliensis), Sábalo (Prochilodus lineatus), Boga (Leporinus obtusidens), Patí (Luciopimelodus pati), Armado común (Pterodoras granulosus), Tararira (Hoplias malabaricus), Vieja de látigo (Paraloricaria vetula), Otras viejas (Loricariiae), Bagre negro (Rhamdia quelen), Otros bagres, armados, etc. (Siluriformes), Palometa negra (Serrasalmus maculatus), Piraña palometa (Pygocentrus nattereri), etc. Y el otro sobre diversidad, cargas parasitarias y su posible relación con biocidas en peces. En el cual se estudiaron cinco especies principales de peces: Dorado (Salminus brasiliensis), Sábalo (Prochilodus lineatus), Boga (Leporinus obtusidens), Patí (Luciopimelodus pati) y Vieja de látigo (Paraloricaria vetula). Las muestras se obtuvieron de material colectado en campañas de muestreo en el embalse de Salto Grande y aguas debajo de la represa. Se hallaron diferentes grupos de parásitos en todas las especies seleccionadas para el estudio así como algunas especies de caractrer zoonótico. Se calculó la prevalencia, intensidad y abundancia de infección para cada parásito.
        Mixta
        10 horas semanales
        Facultad de Veterinaria, Regional Norte - Salto, UDELAR, Departamento de Parasitología Veterinaria , Coordinador o Responsable
        Equipo: CASTRO, O. , FÉLIX, M.L. , CARNEVIA, D. , PERRETTA, A. , VENZAL, J.M. , MELÉNDREZ, A. , LEITES, V. , GARCÍA DA ROSA, E. , LAIRIHOY, R.
        Palabras clave: Parásitos Zoonosis parasitarias Peces Represa Salto Grande Biocidas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

    • Proyectos de investigación y desarrollo

      • Caracterización molecular de las especies de Lymnaea presentes en el Uruguay. (03/2016 - 03/2017 )

        Fuente financiera: Proyectos de Investigación CIDEC 2016, Facultad de Veterinaria. Monto del apoyo: 58.300 pesos uruguayos.
        5 horas semanales
        Facultad de Veterinaria, UdelaR , Laboratorio de Vectores y Enfermedades transmitidas
        Investigación
        Integrante del Equipo
        Concluido
        Financiación:
        Facultad de Veterinaria, Uruguay, Apoyo financiero
        Equipo: CASTRO, O. (Responsable) , CORREA, O. , SANCHÍS, J. , ARMúA, M.T. (Responsable)
        Palabras clave: Uruguay Lymnaea
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Caracterización molecular de Borrelia burgdorferi, agente de la enfermedad de Lyme, en garrapatas del complejo Ixodes ricinus en Uruguay (03/2013 - 05/2015 )

        Código del Proyecto: FMV_2_2011_1_6555 Fuente financiera: Proyectos de Investigación Aplicada (Fondo María Viñas 2011), Agencia Nacional de Investigación e Innovación (ANII). Duración: 2013-2015. Monto del apoyo: 521.500 pesos uruguayos.
        10 horas semanales
        Facultad de Veterinaria, UdelaR , Departamento de Parasitología Veterinaria
        Investigación
        Coordinador o Responsable
        Concluido
        Financiación:
        Agencia Nacional de Investigación e Innovación, Uruguay, Apoyo financiero
        Equipo: FÉLIX, M.L. , MAYA, L. , COLINA, R. , CARVALHO
        Palabras clave: Garrapatas Enfermedad de Lyme Salud pública y animal
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /

      • Investigación sobre los ectoparásitos de carnívoros de importancia sanitaria en la ciudad de Bella Unión (10/2012 - 12/2013 )

        Programa de Apoyo a la Investigación Estudiantil - 2012
        5 horas semanales
        Regional Norte - Salto, UdelaR , Departamento de Parasitología Veterinaria
        Otra
        Coordinador o Responsable
        En Marcha
        RRHH formados en el proyecto:
        Pregrado:3
        Financiación:
        Comisión Sectorial de Investigación Científica, Uruguay, Apoyo financiero
        Equipo: RADCENCO, P. , CARBALLAL, P. (Responsable) , GALLIAZZI, R.
        Palabras clave: importancia sanitaria perros ectoparásitos Bella Unión
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Aspectos sanitarios de la fauna íctica de la región de Salto Grande (06/2011 - 07/2013 )

        Fuente financiera: Convenio Específico Universidad de la República - Comisión Técnica Mixta Salto Grande. Aspectos Sanitarios de la Fauna Ictica de la Región de Salto Grande (Nº expediente 131140-001164-11). Monto del apoyo: 280.000 pesos uruguayos.
        10 horas semanales
        Facultad de Veterinaria, Regional Norte - Salto, UDELAR , Departamento de Parasitología Veterinaria
        Investigación
        Coordinador o Responsable
        Concluido
        Equipo: CASTRO, O. , FÉLIX, M.L. , CARNEVIA, D. , VENZAL, J.M. (Responsable) , LEITES, V. , GARCÍA DA ROSA, E. , LAIRIHOY, R.
        Palabras clave: Parásitos Zoonosis parasitarias Peces Represa Salto Grande Biocidas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
        Ciencias Agrícolas / Agricultura, Silvicultura y Pesca / Pesca /

      • Muestreo serológico de hospedadores domésticos y silvestres de Amblyomma triste en zonas endémicas de rickettsiosis humana en Uruguay (01/2012 - 01/2013 )

        La ejecución de este proyecto fue sumamente productiva, permitiendo no sólo cumplir con los objetivos del mismo, sino que ha sido de gran colaboración para el desarrollo de la Tesis de Maestría de la Lic. Paula Lado. De acuerdo a los objetivos planteados, los resultados obtenidos fueron los siguientes: Objetivo general: Realizar un muestreo serológico en perros y roedores para la detección mediante inmunofluorescencia indirecta de rickettsias con antígenos regionales de Rickettsia spp. en zonas endémicas a rickettsiosis humana en Uruguay. Se realizaron diversas salidas de campo, lográndose colectar muestras de sangre de 8 roedores, y se formó una seroteca de muestras de 1000 caninos domésticos. La totalidad de las muestras fue procesada por Inmunofluorescencia Indirecta para la detección de anticuerpos Anti-Rickettsia spp. Objetivos Específicos: - Determinar la prevalencia de infección de R. parkeri en caninos domésticos de la zona endémica a rickettsiosis de Uruguay. Se determinó una prevalencia de 20.3% de Rickettsia spp. en la población canina de estudio, ya que 203 perros de los 1000 analizados presentaron seropositividad para al menos uno de los antígenos regionales empleados. - Determinar la prevalencia de infección de R. parkeri en roedores, hospederos de estadios inmaduros de A. triste de la zona endémica a rickettsiosis de Uruguay. Se lograron colectar únicamente 9 roedores en 3 salidas de campo. Se pudo obtener sangre de 8 de ellos, y por lo tanto se realizó la técnica serológica en este número de individuos. Así, 2/8 (25%), fueron seroreactivos para Rickettsia spp. A su vez, a partir de las titulaciones realizadas, la especie de patógeno se identificó como R. parkeri, en los mismos animales, por lo que la frecuencia de ocurrencia fue de 25%. Los dos roedores seropositivos, expuestos entonces a R. parkeri, se identificaron como Scapteromys tumidus. - Correlacionar la dinámica de infección a lo largo de un año en las muestras de los hospedadores a estudiar. En el caso de los roedores esto no pudo realizarse debido a la baja eficiencia de captura. En el caso de los caninos, las 1000 muestras colectadas correspondieron a las diferentes estaciones del año, y el número es considerable, por lo que se realizó un análisis estadístico, empleando Chi-cuadrado, para determinar si existió una diferencia significativa en la proporción de seropositivos en las diferentes estaciones. Así, se logró determinar que hay un acúmulo de caninos seropositivos en la primavera (32%), con respecto a las restantes épocas del año (14.9% - 16.6%). Como mencionamos más arriba, este trabajo ha permitido generar información muy valiosa a la epidemiología de la rickettsiosis en el área endémica de nuestro país, y a su vez ha sido de sustancial ayuda en la formación de Recursos humanos, a través de la utilización de las muestras en una Tesis de posgrado.
        10 horas semanales
        Facultad de Veterinaria, UdelaR , Departamento de Parasitología Veterinaria
        Investigación
        Coordinador o Responsable
        Concluido
        RRHH formados en el proyecto:
        Maestría/Magister:1
        Financiación:
        Facultad de Veterinaria, Uruguay, Apoyo financiero
        Equipo: LADO, P. (Responsable)
        Palabras clave: Amblyomma triste Rickettsia parkeri Serología Rickettsiosis humana
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Parasitosis de potencial zoonótico en peces de la región de Salto Grande (07/2010 - 11/2011 )

        El embalse de Salto Grande y las zonas próximas del Río Uruguay cuentan con una rica fauna ictícola que motiva la existencia de una activa presencia de pescadores deportivos y artesanales que comercian el producto de su pesca en la población de la ciudad. Los peces pueden sufrir patologías de relevancia sanitaria para la especie hospedadora, para los animales domésticos e incluso para la salud humana. Muchas de estas zoonosis ictioparasitarias han sido poco o nada estudiadas no solo en la zona de la región de Salto Grande sino en Uruguay. Este proyecto pretende estudiar en las principales especies de peces que se consumen en la región, la fauna de parásitos que estos albergan y determinar cuáles son zoonóticos. También se estudiarán los demás parásitos que alojan los peces y las injurias inducidas por estos en sus hospederos. Al ser la zona del embalse de Salto Grande, un ecosistema artificial relativamente reciente, en el que los peces y su fauna asociada aún pueden no encontrarse en un estado de equilibrio, el efecto de las parasitosis puede repercutir significativamente en la salud de los peces. Los carnívoros domésticos próximos a la zona de estudio que tengan probabilidades de alimentarse con pescado crudo o sus restos también serán estudiados mediante coprología ya que son susceptibles de albergar diversos parásitos transmitidos por los mismos. Este proyecto posee aspectos relevantes en cuanto a ciencias básicas, salud y también en lo referente a conservación de poblaciones ictícolas ya que busca obtener información acerca de la implicancia de las ictioparasitosis en la salud humana y animal.
        10 horas semanales
        Facultad de Veterinaria, Universidad de la República , Departamento de Parasitología Veterinaria
        Investigación
        Coordinador o Responsable
        Concluido
        RRHH formados en el proyecto:
        Pregrado:1
        Equipo: LAIRIHOY, R. , GARCÍA DA ROSA, E. , CASTRO, O. (Responsable) , FÉLIX, M.L. , CARNEVIA, D. , PERRETTA, A. (Responsable) , VENZAL, J.M. (Responsable) , MELÉNDREZ, A.
        Palabras clave: Zoonosis parasitarias Peces Salto Grande Río Uruguay
        Areas de conocimiento:
        Ciencias Agrícolas / Agricultura, Silvicultura y Pesca / Pesca / Parasitología
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

      • Efectividad del tratamiento periódico con triclabendazole en vacunos sobre la ganancia de peso y lesiones hepáticas en frigorífico. (11/2009 - 10/2010 )

        La fascioliasis en América Latina, y especialmente en Uruguay es una importante enfermedad endémica. Los daños más notorios provocados por muerte de los animales son sólo una fracción de las pérdidas económicas que produce el estado subclínico y crónico de la enfermedad, y que se manifiesta en reducción de la producción de carne, lana y leche, decomisos de hígados, etc. El objetivo del presente trabajo fue medir el efecto del tratamiento mensual con un producto fasciolicida (triclabendazole) en animales naturalmente infestados por Fasciola hepatica sobre la ganancia de peso y estimar a nivel de Frigorífico la presencia de lesiones en los hígados y de F. hepatica adulta en los canalículos biliares. MATERIALES Y MÉTODOS. A) Área experimental: el establecimiento está ubicado en la 7ma. Sección policial del departamento de Paysandú, paraje “Parada Daymán” (31º29´S, 57º52´O). El establecimiento es históricamente endémico a F. hepatica y en el mismo se han hallado caracoles Lymnaea viatrix, hospedadores intermediarios de dicho trematodo. B) Población a estudiar: 30 vacas adultas raza Hereford, con un peso inicial de 480 kilos de promedio, divididas en dos grupos de 15 animales. 24 terneros con un peso promedio de 200 kilos, divididos en dos grupos. C) Metodología de campo: un grupo de vacas y uno de terneros fue dosificado mensualmente con triclabendazole, (Saguaymic, Microsules, 12 mg/kg) frente a los otros grupos sin dosificar. Los animales fueron pesados al inicio y final de la prueba, y se recolectan muestras de materia fecal mensualmente. D) Procesamiento de laboratorio y frigorífico: una vez en el laboratorio las muestras de materia fecal fueron procesadas mediante técnicas de sedimentación a tiempo controlado. En Frigorífico, se procedió a observar la presencia de lesiones hepáticas encontradas en los animales, así como el hallazgo de F. hepatica adulta en los canalículos biliares. RESULTADOS y DISCUSIÓN. Todos los animales presentaron huevos de F. hepatica al inicio de la prueba, los siguientes muestreos fueron negativos a la presencia de huevos en el grupo tratados, mientras que los del grupo testigo, presentaron huevos de manera errática en las sucesivas pruebas. Las vacas del grupo tratado mostraron una pequeña ganancia de peso superior a los del grupo testigo, 29 kilos contra 24 del grupo testigo, sin diferencia significativa. Los terneros del grupo testigo y el grupo tratado no mostraron diferencias en ganancia de peso en el período que duró la prueba. En frigorífico, cinco vacas del grupo testigo presentaban fasciola adulta en sus canalículos, así como huevos en la vesícula biliar de estos animales. Todo el grupo presentaba lesiones de importancia en sus hígados. En el grupo tratado no se encontraron F. hepatica adulta al momento de la faena, pero presentaban lesiones importantes como fibrosis y engrosamiento de dichos canalículos en todos los animales.
        2 horas semanales
        Facultad de Veterinaria, Universidad de la República , Departamento de Parasitología Veterinaria
        Investigación
        Integrante del Equipo
        Concluido
        Equipo: VENZAL, J.M. , SANCHÍS, J. (Responsable) , FÉLIX, M.L.
        Palabras clave: Uruguay fasciolosis triclabendazole
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Conservando los bosques del Río Uruguay al Norte de Salto Grande (01/2010 - 08/2010 )

        La creación del lago de inundación de la represa hidroeléctrica de Salto Grande en 1979 eliminó la mayor parte de los ecosistemas boscosos ribereños e insulares del Río Uruguay al Norte de la represa. En estos bosques se encuentran especies tropicales propias de regiones y climas más norteños. Las semillas de algunos vegetales llegan arrastradas por la corriente del río, y algunos animales, como los murciélagos fruteros y decenas de invertebrados, sobreviven gracias al desarrollo de la vegetación selvática. Muchas de estas especies tienen una distribución restringida en el país. Estudios a nivel nacional han identificado la zona como de alta prioridad para la conservación y a nivel internacional es considerada un área de importancia para la conservación de las aves. Este proyecto propone involucrar a la comunidad local en la generación de una propuesta de ingreso del área al Sistema Nacional de Áreas Protegidas. Dicha propuesta apunta a reunir la información disponible y cubrir vacíos de información respecto a flora, fauna, geografía y valores culturales del área, proponiendo directrices preliminares para la creación de un área protegida. Se plantea trabajar con actores del medio rural, del Pueblo Colonia Palma (Dpto. de Artigas) y de la Ciudad de Salto, capital del Dpto. homónimo, donde existe una Regional de la Universidad de la República que participa como socio en este emprendimiento.
        2 horas semanales
        CENTRO DE ESTUDIOS DE CIENCIAS NATURALES Prof. Francisco Oliveras
        Investigación
        Integrante del Equipo
        Concluido
        Financiación:
        Institución del exterior, Apoyo financiero
        Equipo: OCAMPO, F. , GONZÁLEZ, E.M. , BOTTO, G. (Responsable) , BAZZANO, V. , RODALES, A.L. (Responsable) , BESSONART, J. , BOBADILLA, P. , CLAVIJO, C. , CORTÉS, G. , COLINA, M. , GAGO , HERNÁNDEZ, D. , LARA, L. , SERRA, S.

      • Enfermedades emergentes en Uruguay: Rickettsia parkeri en Amblyomma triste, su prevalencia, y estacionalidad del vector en el sur uruguayo. (01/2004 - 01/2005 )

        Las garrapatas son importantes vectores de enfermedades para los animales y el hombre. En el Uruguay, se han diagnosticado algunas enfermedades en el hombre transmitidas por garrapatas como ser Fiebre botonosa por Rickettsia conorii, Ehrlichiasis y enfermedad de Lyme, pero estos diagnósticos estaban basados en la presencia de anticuerpos o en la presencia de signos clínicos. Recientemente, mediante PCR se identificó Rickettsia parkeri en garrapatas Amblyomma triste de Uruguay, una de ellas se había extraído de un paciente humano al cual le fue diagnosticada una rickettsiosis clínica. Esto sugiere que la Rickettsia actuante en los casos clínicos de Uruguay sería R. parkeri y no R. conorii como se había propuesto anteriormente. Esta Rickettsia es la primera vez que se asocia a casos humanos de rickettsiosis, por lo que probablemente se pueda tratar de una enfermedad emergente. El objetivo de este proyecto es estudiar la estacionalidad del vector de esta enfermedad (A. triste) por un año, y determinar la prevalencia de Rickettsia parkeri en el mismo. Mensualmente, se colectarán adultos de A. triste mediante trampas de arrastre en los departamentos de Montevideo y Canelones desde enero de 2004 hasta diciembre de 2004. Se determinará él o los meses de mayor abundancia de estas garrapatas en el campo. Los adultos se enviarán, a España para ser procesados por PCR e identificar la presencia de R. parkeri en los mismos.
        10 horas semanales
        Facultad de Veterinaria , Departamento de Parasitología Veterinaria
        Investigación
        Coordinador o Responsable
        Concluido
        Equipo: CASTRO, O. , CABRERA, P.A. , DE SOUZA, C.G.
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

      • Guía de las garrapatas del género Ixodes (Acari: Ixodidae) en Uruguay y confección de una colección de referencia (01/2001 - 01/2002 )

        Las garrapatas son ectoparásitos hematófagos de animales y el hombre, con gran importancia en la transmisión de diversos agentes patógenos. El género Ixodes es el más amplio dentro de la familia Ixodidae (garrapatas duras) con unas 240 especies, distribuidas en 14 subgéneros. Desde no hace mucho tiempo a las especies de dicho género se las ha venido estudiando en diversos países, debido a que son las principales vectoras de la Enfermedad de Lyme, enfermedad emergente, causada por Borrelia burgdorferi. En Uruguay no existen trabajos sobre dicha enfermedad, pero se ha llevado a cabo una revisión reciente sobre el género, que indica que en nuestro país el mismo estaría representado por Ixodes loricatus, Ixodes pararicinus, Ixodes auritulus e Ixodes longiscutatum, o sea cuatro especies, ya que según la bibliografía serían cinco, pero I. uruguayensis demostró ser sinónimo de I. longiscutatum. Estos estudios incluyeron, además, nuevos hospedadores, ampliación de la distribución y algunos aspectos de la biología de estas garrapatas. El principal objetivo de este trabajo es crear una guía fotográfica (tanto óptica como de barrido) y una clave para facilitar el reconocimiento de un género tan complejo como lo es Ixodes, aprovechando lo resultados obtenidos recientemente por nuestro equipo de investigación. También es de interés la formación de una colección de referencia sobre el género en nuestro país (casi inexistentes en América), ya que se cuenta con material suficiente, además de haber realizado intercambios con el exterior para enriquecer a la misma. Para esto hacen falta materiales fungibles, realizar algunas salidas de campo a zonas ya preestablecidas para la colecta de nuevos ejemplares e ingresar los datos a una base informatizada.
        10 horas semanales
        Facultad de Veterinaria , Departamento de Parasitología Veterinaria
        Investigación
        Coordinador o Responsable
        Concluido
        Equipo: DE SOUZA, C.G. , CASTRO, O. , MORGADES, D. , FÉLIX, M.L. , CASAS, L. , CABRERA, P.
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología

      • Inmunización contra el origen felino del ciclo de la toxoplasmosis (01/2000 - 01/2001 )

        
        20 horas semanales
        Facultad de Veterinaria , Departamento de Parasitología Veterinaria
        Investigación
        Coordinador o Responsable
        Concluido
        Financiación:
        Comisión Sectorial de Investigación Científica, Uruguay, Apoyo financiero
        Equipo: FREYRE, A. (Responsable) , FALCÓN, D.
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

    • Docencia

      • Doctor en Medicina y Tecnología Veterinaria* (02/2008 - a la fecha)

        Grado
        Responsable
        Asignaturas:
        Parasitología y Enfermedades Parasitarias. Regional Norte-Sede Salto, 40 horas, Teórico-Práctico
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología y Enfermedades Parasitarias

      • Maestría en Salud Animal (07/2009 - a la fecha)

        Maestría
        
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Programa de Posgrado en Salud Animal, Fvet (09/2018 - 09/2018 )

        Maestría
        Invitado
        Asignaturas:
        Medicina de la Conservación, 45 horas, Teórico
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Maestría en Salud Animal (10/2017 - 12/2017 )

        Maestría
        Organizador/Coordinador
        Asignaturas:
        Diagnóstico molecular de enfermedades transmitidas por vectores de importancia médica y veterinaria., 10 horas, Teórico-Práctico

      • Maestría en Salud Animal (10/2017 - 10/2017 )

        Maestría
        Organizador/Coordinador
        Asignaturas:
        Taxonomía y métodos de estudios de garrapatas y enfermedades transmitidas en el Cono Sur de América, 40 horas, Teórico-Práctico
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

      • Maestría en Salud Animal (08/2016 - 11/2016 )

        Maestría
        Organizador/Coordinador
        Asignaturas:
        Curso de Posgrado en Salud Animal y Producción Animal: “Diagnóstico de las helmintiasis y eimeriosis en rumiantes”., 8 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Curso de Parasitología y enfermedades parasitarias, Plan 1998, Facultad de Veterinaria, CENUR Litoral Norte (03/2009 - 06/2015 )

        Grado
        Asistente

      • Doctor en Medicina y Tecnología Veterinaria* (08/2003 - 01/2008 )

        Grado
        Asistente
        Asignaturas:
        Parasitología y Enfermedades Parasitarias, 40 horas, Teórico-Práctico
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología y Enfermedades Parasitarias

      • Doctor en Medicina y Tecnología Veterinaria* (01/2000 - 08/2003 )

        Grado
        Asistente
        Asignaturas:
        Parasitología y Enfermedades Parasitarias, 20 horas, Teórico-Práctico
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología y Enfermedades Parasitarias

    • Extensión

      • Colaboración con los veterinarios encargados de la parte sanitaria de los animales de la protectora PRODEA, mediante la realización de análisis coproparasitarios de muestras remitidas de perros pertenecientes a esta organización. (01/2010 - a la fecha )

        Facultad de Veterinaria, Regional Norte - Salto, UDELAR, Departamento de Parasitología Veterinaria
        2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Análisis parasitarios de animales del Zoológico Municipal de Salto. Realización de análisis coproparasitarios de muestras provenientes de animales del Zoológico Municipal de Salto como parte del convenio que existe entre ambas instituciones. (01/2010 - a la fecha )

        Facultad de Veterinaria, Regional Norte - Salto, UDELAR, Departamento de Parasitología Veterinaria
        2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • (07/2013 - 07/2013 )

        Universidad de la República, Área Extensión, Facultad de Veterinaria
        5 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Proyecto: Trematodosis (Fasciola hepatica y Paramphistomum spp.) en tambos de la cuenca lechera de Salto. Convocatoria: Proyectos y Actividades de Extensión 2009 – 2010. Línea 4: Actividades de Extensión Universitaria. (09/2009 - 09/2010 )

        Facultad de Veterinaria, Regional Norte-Sede Salto, Departamento de Parasitología Veterinaria
        5 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología y Enfermedades Parasitarias

      • Organización y coordinación del Curso: Actualización sobre garrapatas y enfermedades transmitidas de interés médico y veterinario. Regional Norte - Sede Salto. 24 y 25 de julio 2009 (07/2009 - 07/2009 )

        Regional Norte - Sede Salto, Unidad de Educación Permanente Regional Norte
        40 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología y Enfermedades Parasitarias

      • Entrevista al Semanario Búsqueda sobre garrapatas de perros del Uruguay. 10 de enero de 2002. Publicado en Búsqueda Año XXXI Nº 1133, pág. 32. (01/2002 - 01/2002 )

        2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Curso: Introducción a la Parasitología de los Animales Silvestres. 16 al 23 de octubre de 2001 (10/2001 - 10/2001 )

        Facultad de Veterinaria, Departamento de Parasitología Veterinaria, Grupo Tamanduá y Vida Silvestre
        20 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología y Enfermedades Parasitarias

      • Participación en la organización de las Jornadas sobre animales silvestres, desarrollo sustentable y medio ambiente (08/2000 - 08/2000 )

        Facultad de Veterinaria, Montevideo, Facultad de Veterinaria
        10 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Colaborador en el Proyecto de Pasantía en la Estación de Cría de Fauna Autóctona de Pan de Azúcar (11/1999 - 11/1999 )

        Facultad de Veterinaria, Montevideo, Comisión Ambientalista
        2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Colaboración en las Jornadas de Discusión sobre alternativas y metodologías de las pasantías a desarrollarse en la Estación de cría del Cerro Pan de Azúcar, Maldonado (08/1998 - 08/1998 )

        Facultad de Veterinaria, Montevideo, Facultad de Veterinaria
        2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

    • Pasantías

      • (07/2008 - 09/2008 )

        Facultad de Veterinaria, UNiversidad de Zaragoza, España., Unidad de Parasitología y Enfermedades Parasitarias
        40 horas semanales
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • (09/2007 - 12/2007 )

        Facultad de Veterinaria, UNiversidad de Zaragoza, España., Unidad de Parasitología y Enfermedades Parasitarias
        40 horas semanales
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • (02/2003 - 02/2003 )

        Instituto Nacional de Tecnología Agropecuaria, Rafaela, Santa Fe, Argentina, INTA Rafaela
        40 horas semanales
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • (11/2002 - 12/2002 )

        Facultad de Veterinaria, UNiversidad de Zaragoza, España., Unidad de Parasitología y Enfermedades Parasitarias
        40 horas semanales
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

    • Otra actividad técnico-científica relevante

      • Viaje de reunión de coordinadores de la Red Iberoamericana de Investigación y Estudio de las Enfermedades Rickettsiales (RIICER) financiada por el programa Iberoamericano de Ciencia y Tecnología para el desarrollo (CYTED). Montería, Córdoba, Colombia. (12/2010 - 12/2010 )

        RIICER - CYTED
        2 horas semanales
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas / Rickettsiosis

      • Viaje de reunión de coordinadores de la Red Iberoamericana de Investigación y Estudio de las Enfermedades Rickettsiales (RIICER) financiada por el programa Iberoamericano de Ciencia y Tecnología para el desarrollo (CYTED). Mérida, Yucatán, México. (06/2010 - 06/2010 )

        RIICER - CYTED
        2 horas semanales
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas / Rickettsiosis

      • Colaborador de investigación en Toxoplasmosis. Tareas de Infecciones experimentales, reproducción de ratas y ratones, experimentos de inoculación intraduodenal que implican anestesia y laparatomía de los animales. Responsable: Dr. Álvaro Freyre (01/1999 - 01/2001 )

        Facultad de Veterinaria, Departamento de Parasitología Veterinaria, Laboratorio de Toxoplasmosis
        10 horas semanales
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

    • Gestión Académica

      • Responsable académico de la propuesta Polo de Desarrollo Universitario (PDU) Creación de un Laboratorio de Vectores y Enfermedades Transmitidas en el Interior del Uruguay (01/2013 - a la fecha )

        Universidad de la República, Regional Norte - Salto
        Gestión de la Investigación
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Responsable académico de la propuesta Polo de Desarrollo Universitario (PDU) Creación de un Laboratorio de Vectores y Enfermedades Transmitidas en el Interior del Uruguay (03/2012 - a la fecha )

        Facultad de Veterinaria
        Otros
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

      • Integrante alterno de Facultad de Veterinaria en la Mesa del Área Científica Tecnológica Agraria - Regional Norte (05/2012 - a la fecha )

        Participación en consejos y comisiones

      • Delegado (titular) ante la Comisión Sectorial de Investigación Científica (CSIC) por el Área de Tecnologías y Ciencias de la Naturaleza y el Hábitat, (Exp. 111163-000024-25). (09/2025 - a la fecha )

        Gestión de la Investigación 2 horas semanales

      • Representante (Suplente) de Facultad de Veterinaria en el Grupo Técnico de Trabajo en Miasis por Cochliomya hominivorax (S.F.V. 81/16) (Exp. Nº 110011-001441-16). (11/2016 - a la fecha )

        Universidad de la República, Facultad de Veterinaria
        Participación en consejos y comisiones
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Delegado titular de Facultad de Veterinaria en la Mesa Coordinadora del Área de Tecnologías y Ciencias de la Naturaleza y el Hábitat (CENUR Litoral Norte). Resolución del Consejo de la Facultad de Veterinaria del 9 de mayo de 2019 (Exp. Nº 110011-001563-1 (05/2019 - a la fecha )

        Participación en consejos y comisiones 2 horas semanales

      • Representante titular de Facultad de Veterinaria ante el Grupo de Trabajo en invertebrados terrestres del Comité Nacional de Especies Exóticas Invasoras (CEEI). Grupos de Trabajo del Comité Nacional de Especies Exóticas Invasoras (CEEI)(DINAMA - MVOTMA). (10/2020 - a la fecha )

        Participación en consejos y comisiones 2 horas semanales

      • Integrante del Grupo de Trabajo para el proceso de consolidación del Departamento de Ciencias Veterinarias y Agrarias del CENUR Litoral Norte. Resolución N° 138 del Consejo de la Facultad de Veterinaria de la Universidad de la República. 17 de diciembr (12/2020 - a la fecha )

        Participación en consejos y comisiones 1 horas semanales

      • Representante por Facultad de Veterinaria en la Comisión Espejo del Área de Tecnologías y Ciencias de la Naturaleza y el Hábitat ante la CSIC (Exp. N° 111160-000059-23). (06/2023 - a la fecha )

        Facultad de Veterinaria Participación en consejos y comisiones 1 horas semanales
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Delegado (1er. suplente) ante la Comisión Sectorial de Investigación Científica (CSIC) por el Área de Tecnologías y Ciencias de la Naturaleza y el Hábitat. Exp. 101170-000041-24 (09/2024 - 08/2025 )

        Gestión de la Investigación 2 horas semanales

      • Delegado (2° suplente) ante la Comisión Sectorial de Investigación Científica (CSIC) por el Área de Tecnologías y Ciencias de la Naturaleza y el Hábitat. Período 1.9.23 a 31.8.24 (distribuido N° 779.23) (Exp. 241163-000009-23). (09/2023 - 08/2024 )

        Gestión de la Investigación 2 horas semanales

      • Representante de Facultad de Veterinaria al Comité de Especies Exóticas Invasoras. Resolución del Consejo de la Facultad de Veterinaria del 4 de setiembre de 2014 (Exp. Nº 110011-001151-14). (09/2014 - 10/2017 )

        Participación en consejos y comisiones 1 horas semanales
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Integrante del Grupo de Trabajo para elaborar propuestas tendientes a avanzar en la Consolidación de la Carrera Veterinaria en el Centro Universitario Regional Litoral Norte (07/2015 - 07/2015 )

        Facultad de Veterinaria
        Participación en consejos y comisiones
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Coordinador interino de la carrera de Veterinaria en el Norte (Sede Salto) (Exp. N° 110011-000973-14). (08/2014 - 11/2014 )

        Regional Norte, Facultad de Veterinaria
        Otros

      • Titular por el Área de Tecnologías y Ciencias de la Naturaleza y el Hábitat del Consejo de Regional Norte - Salto (05/2013 - 05/2014 )

        Universidad de la República, Regional Norte - Salto
        Participación en consejos y comisiones

      • Coordinador por Uruguay de la Red iberoamericana para la Investigación y Control de las Enfermedades Rickettsiales (RIICER, Red 210RT0403) apoyada por el Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo (CYTED) (06/2010 - 12/2013 )

        Otros
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas / Rickettsiosis

      • Suplente de la Asamblea del Claustro de Facultad de Veterinaria, UDELAR. Elecciones del 31 de octubre de 2007 (01/2010 - 01/2012 )

        Facultad de Veterinaria
        Participación en cogobierno 2 horas semanales
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Integrante de la Comisión CIDEC (Comisión de Investigación y Desarrollo Científico – Facultad de Veterinaria) (04/2010 - 01/2012 )

        Facultad de Veterinaria, Universidad de la República
        Participación en consejos y comisiones
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Integrante titular de la Comisión Asesora de Regional Norte-Sede Salto (Facultad de Veterinaria) (09/2009 - 09/2010 )

        Facultad de Veterinaria, Regional Norte-Sede Salto
        Participación en consejos y comisiones
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Integrante titular por la Facultad de Veterinaria de la Comisión Asesora de Laboratorio de Regional Norte (03/2009 - 03/2010 )

        Regional Norte - Sede Salto, Facultad de Veterinaria
        Participación en consejos y comisiones
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Suplente por el Orden Docente a la Asamblea del Claustro de Facultad de Veterinaria, UDELAR. (07/2007 - 01/2010 )

        Facultad de Veterinaria, Universidad de la República
        Participación en cogobierno
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Integrande de redacción Proyecto: Mejora del Equipamiento de la Sala de Microscopía de Regional Norte - Sede Salto. Llamado 2009: Mejora de la Enseñanza - Equipamiento de aulas, talleres, laboratorios y clínicas para la enseñanza de grado. CSE. UDELAR (08/2009 - 09/2009 )

        Regional Norte - Sede Salto
        Gestión de la Enseñanza
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Integrante de comisión Ad Hoc para propuesta institucional: Programa de Fortalecimiento del Equipamiento de Investigación en Servicios de la Universidad de la República (CSIC). Proyecto Institucional Modalidad II. Aprobado con 971.005 pesos uruguayos. (02/2009 - 03/2009 )

        Regional Norte - Sede Salto
        Gestión de la Investigación
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

• Sector Educación Superior/Público - Universidad de la República - Uruguay

  Centro Universitario Regional Litoral Norte / Departamento de Ciencias Biológicas

  • Vínculos con la Institución

    • Funcionario/Empleado (07/2015 - a la fecha)Trabajo relevante

      Profesor Agregado 40 horas semanales / Dedicación total
      Escalafón: Docente
      Grado: Grado 4
      Cargo: Efectivo

    • Funcionario/Empleado (11/2013 - 07/2015)Trabajo relevante

      Profesor Agregado 40 horas semanales
      Coordinador del Polo de Desarrollo Universitario (PDU) Laboratorio de Vectores y enfermedades transmitidas. CENUR Litoral Norte - Salto
      Escalafón: Docente
      Grado: Grado 4
      Cargo: Efectivo

  • Actividades

    • Líneas de investigación

      • Garrapatas y enfermedades transmitidas (07/2015 - a la fecha )

        Sub-líneas: 1 - Sistemática de Argásidos e Ixódidos de la Región Neotropical. 2 - Enfermedades transmitidas por garrapatas a humanos, animales domésticos y de producción. 3 - Ecología de garrapatas Neotropicales. Esta es la principal línea de investigación, la cual desde el año 2001 ha generado numerosas publicaciones, muchas de ellas provenientes de resultados obtenidos en proyectos concursables. Si bien inicialmente se trabajó en sistemática de garrapatas en la descripción de nuevas especies utilizando técnicas clásicas, así como moleculares, más recientemente se han determinado una amplia variedad de microrganismos transmitidos por garrapatas de los cuales muchos de ellos son de importancia en la salud animal y pública, con carácter zoonótico. Además de trabajar con investigadores de diferentes Instituciones de Uruguay, la línea sigue manteniendo vínculos estrechos con investigadores extranjeros: INTA Rafaela (Argentina), Universidad de Sao Paulo (Brasil), Instituto Butantan (Brasil), Universidad de Concepción (Chile), Hospital de La Rioja, Logroño (España), Universidad de Zaragoza (España), Universidad Central del Ecuador (Ecuador), Universidad de San Marcos (Perú), entre otras. A modo de ejemplo se destaca la descripción de nuevas especies de garrapatas como: Ornithodoros rioplatensis (Uruguay), Amblyomma boeroi (Argentina), Argas keiransi (Chile) y Antricola guglielmonei (Brasil), totalizando más de 20 nuevas especies descriptas para Sudamérica. Entre los principales agentes patógenos determinados, el uso de técnicas moleculares como PCR permitió esclarecer el agente de la rickettsiosis humana en Uruguay, Rickettsia parkeri, transmitida por la garrapata Amblyomma triste en los departamentos del sur de Uruguay. También se caracterizó por primera vez la presencia en el hemisferio sur de dos genotipos de Borrelia burdorferi, relacionados con los agentes causantes de la enfermedad de Lyme en el hemisferio norte. Más recientemente se han detectado varias bacterias (Ehrlichia, Anaplasma) y protozoarios (Babesia, Rangelia, Hepatozoon, etc.) tanto en garrapatas como en tejidos de animales silvestres y domésticos.
        Mixta
        10 horas semanales
        Departamento de Ciencias Biológicas, Laboratorio de Vectores y enfermedades transmitidas , Coordinador o Responsable
        Equipo: VENZAL, J.M.

    • Proyectos de investigación y desarrollo

      • Miasis por Cochliomyia hominivorax en fauna silvestre en Uruguay: aportes para el control de una problemática nacional. (01/2025 - a la fecha)

        Código: 22520240100096UD CONVOCATORIA: Programa Proyectos de I+D 2024. Comisión Sectorial de Investigación Científica. UNIVERSIDAD DE LA REPUBLICA Disciplina y subdisciplina: Ciencias Veterinarias - Especialidad: Parasitología Área del conocimiento: Agraria Monto total: $ 1.517.068,44
        5 horas semanales
        Coordinador o Responsable
        En Marcha
        RRHH formados en el proyecto:
        Doctorado:1
        Financiación:
        Agencia Nacional de Investigación e Innovación, Uruguay, Beca
        Comisión Sectorial de Investigación Científica, Uruguay, Apoyo financiero
        Equipo: VENZAL, J.M. (Responsable) , Pérez A.L. (Responsable) , MIRAZO, S. , OLHAGARAY , CASTRO JANER, E. , Porro, A , Arrighetti, F. , Ferreira Peláez, A.R. , A. CRAVINO , Analia Rey González , C. Echaides , Crampet, B , Rohrer Meneses, M.V. , Saravia A. , Vazquez Olivera , C.V. , González Tonelli, E.F. , LOUREIRO, M.A.
        Palabras clave: Cochliomyia hominivorax Epidemiología Parasitología Fauna silvestre
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / NO CORRESPONDE / Parasitología

      • Diagnóstico de hemoparásitos de transmisión vectorial en bovinos y caracterización genética de poblaciones de Rhipicephalus microplus en Uruguay. (01/2024 - a la fecha)

        Código: FPTA 413 Título del Proyecto: Diagnóstico de hemoparásitos de transmisión vectorial en bovinos y caracterización genética de poblaciones de Rhipicephalus microplus en Uruguay. Agencia financiadora: Convocatoria Llamado FONDO DE PROMOCIÓN DE TECNOLOGÍA AGROPECUARIA (FPTA). FPTA 2022 Temas priorizados. Instituto Nacional de Investigación Agropecuaria (INIA). Código del Proyecto: FPTA 413. Responsable: José Manuel VENZAL BIANCHI Período: 2024 al 2027. Presupuesto: US$ 317.830,00 dólares americanos
        5 horas semanales
        Coordinador o Responsable
        En Marcha
        RRHH formados en el proyecto:
        Pregrado:1
        Maestría/Magister:1
        Financiación:
        INIA, Uruguay, Apoyo financiero
        Equipo: VENZAL, J.M. (Responsable) , QUEIROLO, D. , Fresia P , Félix, M.L. , Alvez, R. , Sanchís, J. , Nava, S. , Morel, N. , Reck, J. , López, F. , Gómez, X. , Cortés, M.
        Palabras clave: bovinos garrapata estructura genética resistencia hemoparásitos diagnóstico
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / NO CORRESPONDE / Parasitología

      • Caracterización molecular de piroplasmas en garrapatas del complejo Ixodes ricinus de las regiones Litoral Norte y Noreste de Uruguay: implicancias para la salud animal y pública. (01/2024 - a la fecha)

        Código: 22420230100032UD Agencia financiadora: Programa de Iniciación a la Investigación 2023, Comisión Sectorial de Investigación Científica, Universidad de la República. Responsable: Rodrigo Alvez Decesari Período: duración del proyecto: 18 meses. Participación: Referente Académico: José M. Venzal Presupuesto: $ 243.900 pesos uruguayos
        2 horas semanales
        Integrante del Equipo
        En Marcha
        RRHH formados en el proyecto:
        Maestría/Magister:1
        Financiación:
        Comisión Sectorial de Investigación Científica, Uruguay, Apoyo financiero
        Equipo: VENZAL, J.M. , Parodi, P , FÉLIX, M.L.
        Palabras clave: Piroplasmas Garrapatas Enfermedades transmitidas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / NO CORRESPONDE / Parasitología
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

      • Determinación de la situación actual de la garrapata Rhipicephalus microplus y tristeza parasitaria y control integrado de ambas enfermedades. (08/2016 - 11/2020 )

        En Uruguay se estima que la garrapata (Rhipicephalus microplus) causa pérdidas económicasestimadas en 37 a 45 millones de dólares, que incluyen las ocasionadas por tristeza parasitaria(babesiosis y anaplasmosis). La alta frecuencia de tristeza parasitaria se debe, principalmente, a queUruguay está situado en una zona considerada de inestabilidad enzoótica. A pesar de de haberresultados de investigación y existir el marco legal para el control de garrapata el principal obstáculopara el éxito del control ha sido el de resistencia de R. microplus a los acaricidas utilizados. Esteproyecto pretende, mediante una encuesta, el diagnóstico sistemático de los brotes de tristezaparasitaria y la aplicación de planes de control o erradicación generar conocimiento para mitigar losproblemas mencionados anteriormente y optimizar el uso racional de los acaricidas. Será realizadauna encuesta a aproximadamente 650 productores, elegidos aleatoriamente, durante un muestreode carácter nacional. En esos establecimientos se tomarán muestras de sangre para un estudioserológico para Babesia spp. y Anaplasma spp. Para B. bovis y B. bigemina se realizara la pruebade IFI o ELISA y para A. marginale el Card Test. Para el estudio de los focos de tristeza parasitariaserán realizadas visitas a todos los establecimientos donde esté ocurriendo la enfermedad, que serádiagnosticada por observación de hemoparásitos, hematocrito y pruebas moleculares (MultiplexPCR). Las muestras que resulten positivas para A. marginale, se les realizará un segundo PCR parala amplificación del gen MSP1? para su utilización en la caracterización de las distintas cepas y enla construcción de árboles filogenéticos. Los productos amplificados se purificarán y seránsecuenciados. Será realizado muestreo serológico en 30 animales por establecimiento, paradeterminar el riesgo de tristeza parasitaria y la necesidad de vacunación. Será realizado diagnósticode resistencia mediante las técnicas de inmersión de larvas, test del paquete de larvas y test deadultos. Serán realizados, también, análisis moleculares para la detección de resistencia engarrapatas mediante identificación de las mutaciones en R. microplus asociadas a resistencia apiretroides sintéticos y fipronil. Se planificarán y ejecutarán planes de control o erradicación en 30establecimientos en el área de influencia de INIA Tacuarembó y 30 en el área de influencia de laUDELAR de Salto. En el primer año se establecerá un plan de control basado en el tratamientogeneracional y muestreo serológico para evaluar la situación de babesiosis y anaplasmosis y lanecesidad de vacunar contra estas enfermedades. Se realizará diagnóstico de resistencia mediantelas técnicas mencionadas anteriormente. En el segundo año se definirá si se continúa con el plan decontrol o se pasa a un plan de erradicación. El plan de control estará basado en el tratamientogeneracional que dependerá de la situación de cada establecimiento (instalaciones, históricos deusos de principios activos y diagnósticos previos de resistencia). Se estimarán los posiblesbeneficios económicos del control o erradicación comparando los gastos por garrapaticidas, antes ydespués del inicio del proyecto, y las pérdidas económicas asociadas a tristeza parasitaria
        2 horas semanales
        Instituto Nacional de Investigación Agropecuaria (INIA) , Código del Proyecto: CL_35.
        Investigación
        Integrante del Equipo
        Concluido
        RRHH formados en el proyecto:
        Maestría/Magister:1
        Doctorado:1
        Financiación:
        INIA, Uruguay, Apoyo financiero
        Equipo: VENZAL, J.M. , Riet-Correa F. (Responsable)
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Caracterización molecular de poblaciones de caracoles del género Galba (Lymnaea) en predios con focos de fasciolosis localizados en el litoral norte. (03/2017 - 03/2018 )

        Programa: Programa de Apoyo a la Investigación Estudiantil ? 2017 (PAIE) Número ID del proyecto: 58
        2 horas semanales
        CENUR Litoral Norte - Salto, UdelaR , Laboratorio de Vectores y Enfermedades transmitidas
        Coordinador o Responsable
        Concluido
        RRHH formados en el proyecto:
        Pregrado:1
        Financiación:
        Comisión Sectorial de Enseñanza, Uruguay, Apoyo financiero
        Equipo: PONS, MV (Responsable) , VENZAL, J.M. , ARMUA-FERNANDEZ, M. T.; ARMUA, M.T. (Responsable) , Villamayor, R.
        Palabras clave: Galba (Lymnaea) fasciolosis Caracterización molecular
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Estudio de la presencia de Hepatozoon spp. en zorros atropellados en rutas del noroeste de Uruguay. (03/2017 - 03/2018 )

        Programa: Programa de Apoyo a la Investigación Estudiantil ? 2017 (PAIE). Número ID del proyecto: 60. Hepatozoonosis es una parasitosis causada por protozoarios apicomplexos del género Hepatozoon. Unas 50 especies parasitan mamíferos, y recientemente en Uruguay se ha reportado la presencia de Hepatozoon sp. en Cerdocyon thous (zorro de monte). El objetivo de este estudio fue determinar la presencia de Hepatozoon spp. mediante técnicas de biología molecular en zorros atropellados en rutas del noroeste de Uruguay. Para ello se obtuvieron muestras de sangre y tejido (bazo) de 25 zorros: 14 de C. thous (zorro de monte) y 11 de Lycalopex gymnocercus (zorro de campo). El ADN de las muestras fue extraído mediante un kit comercial. El diagnóstico molecular se realizó mediante la técnica reacción en cadena de la polimerasa (PCR) usando dos set de primers (solapantes) que amplifican una región del gen 18S rARN de Hepatozoon spp. Los productos de PCR fueron purificados y enviados a secuenciar. Las secuencias nucleotídicas obtenidas se compararon utilizando la herramienta BLASTn con otras secuencias de Hepatozoon disponibles en GenBank. También se estudiaron las relaciones filogenéticas a través de la construcción de un árbol filogenético utilizando el software MEGA 7. Seis de las 25 muestras procesadas, cuatro de C. thous y dos de L. gymnocercus (prevalencia: 24%), fueron positivas a Hepatozoon sp. Las secuencias resultaron ser homólogas entre un 97-98% con secuencias de Hepatozoon sp. de C. thous de Brasil. En el análisis filogenético, nuestras muestras formaron un clado con alto soporte con Hepatozoon sp. Curupira 2 obtenida de C. thous de Brasil, y con H. americanum de Estados Unidos.
        5 horas semanales
        CENUR Litoral Norte -Salto, UdelaR , Laboratorio de Vectores y Enfermedades transmitidas
        Otra
        Coordinador o Responsable
        Concluido
        RRHH formados en el proyecto:
        Pregrado:1
        Financiación:
        Comisión Sectorial de Enseñanza, Uruguay, Apoyo financiero
        Equipo: VENZAL, J.M. (Responsable) , da Costa Artave, A.Ch. (Responsable) , ARMUA-FERNANDEZ, M. T.; ARMUA, M.T. , Burutarán, M.
        Palabras clave: Parasitología Hepatozoon Carnívoros silvestres
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Detección de patógenos rickettsiales transmitidos por garrapatas de interés sanitario para humanos y mascotas en Uruguay. (04/2015 - 04/2017 )

        Código del Proyecto: ID 396 Fuente financiera: Comisión Sectorial de Investigación Científica: Proyectos I+D CSIC 2014. Monto del apoyo: 734.070 pesos uruguayos.
        10 horas semanales
        CENUR Litoral Norte – Salto, UdelaR, Salto , Laboratorio de Vectores y Enfermedades transmitidas
        Investigación
        Coordinador o Responsable
        Concluido
        RRHH formados en el proyecto:
        Maestría/Magister:1
        Financiación:
        Comisión Sectorial de Investigación Científica, Uruguay, Apoyo financiero
        Equipo: ARMúA, M.T. , SOSA, N. , CARVALHO, L.
        Palabras clave: Garrapatas Carnívoros Enfermedades rickettsiales humanos
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /

      • Caracterización molecular de las especies de Lymnaea presentes en el Uruguay. (03/2016 - 03/2017 )

        Proyectos de Investigación CIDEC 2015, Facultad de Veterinaria. Responsables: Oscar Castro y María Teresa Armúa. Duración: marzo 2016-marzo 2017. Participación: Colaborador. Monto del apoyo: 58.300 pesos uruguayos.
        2 horas semanales
        Facultad de Veterinaria, UdelaR , Proyectos de Investigación CIDEC 2015
        Investigación
        Integrante del Equipo
        Concluido
        Financiación:
        Facultad de Veterinaria, Uruguay, Apoyo financiero
        Equipo: VENZAL, J.M. , ARMUA-FERNANDEZ, M. T.; ARMUA, M.T. (Responsable) , CASTRO, O. (Responsable)
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Detección molecular de Hepatozoon (Apicomplexa: Hepatozoidae) en ofidios del norte de Uruguay. (01/2015 - 01/2016 )

        Programa: Programa de Apoyo a la Investigación Estudiantil – 2015 (PAIE)
        5 horas semanales
        CENUR Litoral Norte – Salto, UdelaR, Salto , Laboratorio de Vectores y Enfermedades transmitidas
        Otra
        Coordinador o Responsable
        Concluido
        RRHH formados en el proyecto:
        Pregrado:3
        Equipo: BAZZANO, V. (Responsable) , CARVALHO, L. , CRESCIONINI, A. , KUCZURA, N.
        Palabras clave: Uruguay Hepatozoon ofidios
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

    • Dirección y Administración

      • Coordinador del Laboratorio de Vectores y enfermedades transmitidas (07/2015 - a la fecha )

        CENUR Litoral Norte - Salto, Universidad de la Republica 40 horas semanales

    • Docencia

      • Curso Parasitología, plan 2021 Facultad de Veterinaria, CENUR Litoral Norte Salto (08/2023 - a la fecha)

        Grado
        Organizador/Coordinador
        Asignaturas:
        Parasitología, 40 horas, Teórico-Práctico
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / NO CORRESPONDE / Parasitología

      • Semestre 4, Carrera de Veterinaria, Plan 2021, CENUR Litoral Norte (08/2025 - a la fecha)

        Grado
        Organizador/Coordinador
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / NO CORRESPONDE /

      • Introducción a las Ciencias Biológicas II. Ciclo Biología Bioquímica. CENUR Litoral Norte (08/2015 - a la fecha)

        Grado
        Asistente

      • Introducción a la biología animal (08/2015 - a la fecha)

        Grado
        Asistente

      • Curso Enfermedades Parasitarias, plan 2021 Facultad de Veterinaria, CENUR Litorla Norte Salto (03/2022 - a la fecha)

        Grado
        Asistente

      • Curso Parasitología, plan 2021 Facultad de Veterinaria, CENUR Litoral Norte Salto (08/2022 - a la fecha)

        Grado
        Asistente

      • Curso Parasitología y Enfermedades Parasitarias plan 98, Facultad de Veterinaria, CENUR Litoral Norte Salto (03/2016 - 07/2022 )

        Grado
        Asistente

    • Extensión

      • Charla: "Tras la pista de nuevas especies: expediciones a la Amazonia e Islas Galápagos". TODO TIENE SU CIENCIA, Semana de la Ciencia y la Tecnología. 10:30 a 11:00 hrs. Instituto de Alta Especialización (UTU) Misiones 199, Salto, Uruguay. (05/2025 - 05/2025 )

        CENUR Litoral Norte, Laboratorio de Vectores y enfermedades transmitidas
        1 horas

      • Charla: "Cuando la investigación es casi una obsesión: muestreos de campo y garrapatas". 11:00 a 12:00 hrs. - Salón 3, piso 3. Universidad de Puertas Abiertas. 21 y 22 de agosto. Sede Salto - Cenur Litoral Norte - Universidad de la República, Uruguay. (08/2024 - 08/2024 )

        CENUR Litoral Norte, Laboratorio de Vectores y enfermedades transmitidas
        1 horas

      • Charla: "La vocación en la sangre. Viaje a la Amazonia descubriendo nuevas especies". TODO TIENE SU CIENCIA, Semana de la Ciencia y la Tecnología. 09:30 a 10:00 hrs. Instituto de Alta Especialización (UTU) Misiones 199, Salto, Uruguay. (05/2024 - 05/2024 )

        CENUR Litoral Norte, Laboratorio de Vectores y enfermedades transmitidas
        1 horas

      • Charla sobre: Patógenos, vectores y globalización: actualización de la situación de los hemoparásitos en perros en Uruguay,a miembros del Centro Médico Veterinario Paysandú, en la Sede de dicho Centro, calle Uruguay 1189, ciudad de Paysandú, Uruguay. (05/2024 - 05/2024 )

        CENUR Litoral Norte, Laboratorio de Vectores y enfermedades transmitidas
        2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / NO CORRESPONDE /

      • Charla: "Descubriendo nuevas especies en la Amazonia". 14:30 a 15:00 hrs. - Salón 2, piso 3. Universidad de Puertas Abiertas. 8 de setiembre. Sede Salto - Cenur Litoral Norte (09/2022 - 09/2022 )

        Cenur Litoral Norte - Salto, Universidad de la República, Uruguay 1 horas

      • Expositor en el Seminario "Rickettsiosis y enfermedad de Lyme en Uruguay y la región: mitos y verdades". Ciclo de Seminarios Virtuales: "Hablemos de Ciencias" organizados por la SubComisión de Comunicación Académica (SCA) del Departamento de Ciencias Biológicas, CENUR Litoral Norte ? UdelaR. 16 de noviembre de 2021, 11 hrs. Zoom: https://salavirtual-udelar.zoom.us/j/86528346335?pwd=UVlseWQvM2ZTTEZDNzdmRG5RN1VKZz09 (11/2021 - 11/2021 )

        Universidad de la República, Departamento de Ciencias Biológicas, CENUR Litoral Norte
        2 horas

      • Entrevista al Programa: De Humanos y Otros Bichos. Café y Tertulia. Tema: Estudios sobre garrapatas y las enfermedades que transmiten. @BichosCIZ · Emisora de radio. Programa de Radio producido por el Instituto de Investigación en Zoonosis - CIZ, Programa Online de Radio Universitaria Universidad Central del Ecuador. 05 noviembre de 2021, hora 10 (Ecuador). (11/2021 - 11/2021 )

        Universidad Central del Ecuador 2 horas

      • Ponente en el Ciclo de conferencias mensuales y virtuales de la Sociedad Latinoamericana de Acarología sobre: Enfermedades transmitidas por garrapatas. Tema de ponencia: HEMOPARÁSITOS y GARRAPATAS: COMPLEJO DE RHIPICEPHALUS SANGUINEUS y EHRLlCHIOSIS CANINA. Transmisión por Facebook Sociedad Latinoamericana de Acarología -oficial- https://www.facebook.com/groups/988359804947220. 23 de octubre de 2020, 15:00 Costa Rica; 16:00 Colombia, Ecuador, Perú, México (Ciudad de México), Panamá; 17:00 Bolivia, Cuba, República Dominicana; 18:00 Argentina, Brasil (Brasília), Chile, Uruguay. (10/2020 - 10/2020 )

        Sociedad Latinoamericana de Acarología 2 horas

      • Participante invitado en el Ciclo de Conversatorios - 10 Años de la Creación del Laboratorio de Ecología de Enfermedades - Icivet Litoral (CONICET-UNL). Tercer encuentro ?Avances en el conocimiento sobre garrapatas y enfermedades transmitidas por garrapatas en Sudamérica en el siglo XXI?. Miércoles 16 de septiembre - 18:00 hs. Link: https://streamyard.com/e35bn9xpx9 (09/2020 - 09/2020 )

        CONICET - Universidad Nacional del Litoral, Laboratorio de Ecología de Enfermedades - Icivet Litoral
        2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Charla en Jornada Veterinaria. sobre Epidemiología de los hemoparásitos transmitidos por vectores en perros en la región. 25 de octubre de 2019. Lugar: Sede Sociedad Rural Concordia, Entre Ríos, Argentina. (10/2019 - 10/2019 )

        Seccional Médicos Veterinarios, Concordia, Entre Ríos, Argentina. 2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Participante invitado por la Cátedra de Enfermedades Infecciosas, Facultad de Medicina ? Universidad de la República en: Cátedra Abierta con el tema Enfermedades trasmitidas por garrapatas presentado por la Dra. Graciela Pérez. Conexión a distancia mediante programa Zoom. 1 de junio de 2019, Anfiteatro del piso 16, Hospital de Clínicas. (06/2019 - 06/2019 )

        Facultad de Medicina, Universidad de la República, Cátedra de Enfermedades Infecciosas
        2 horas
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /

      • Charla sobre Hemoparásitos transmitidos por vectores en perros en Uruguay para veterinarios, estudiantes y público general. 3 de mayo de 2019, 19.30 horas. Parque Temático Canario - Museo de la Uva y el Vino de la ciudad de Las Piedras, Canelones. (05/2019 - 05/2019 )

        Universidad de la República, Facultad de Veterinaria
        2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Charla sobre Hemoparásitos transmitidos por vectores en perros en Uruguay para veterinarios, estudiantes y público general. 2 de mayo de 2019, 19.30 horas. Anfiteatro del Hospital de Facultad de Veterinaria, Montevideo. (05/2019 - 05/2019 )

        Universidad de la República, Facultad de Veterinaria
        2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Charla sobre Hemoparásitos transmitidos por vectores en perros de caza, para miembros de la Asociación de Cazadores del Uruguay, veterinarios y público en general, 18 de junio de 2018, 19 a 21 horas. Casa de la Cultura, Tranqueras, Rivera. (06/2018 - 06/2018 )

        Asociación de Cazadores del Uruguay 2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Charla sobre Hemoparásitos transmitidos por vectores en perros de caza. 4 de junio de 2018, 19.00 a 21.00 horas en la Comisión de Fomento Jardines de Peñarol, Susana Soca 1825, Montevideo. (06/2018 - 06/2018 )

        Asociación de Cazadores del Uruguay 2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Charla sobre Hemoparásitos transmitidos por vectores en perros de caza, para miembros de la Asociación de Cazadores del Uruguay, veterinarios y público en general. 19 de mayo de 19.30 a 21.30 horas en la Sociedad Rural de Río Branco (Felipe Ferreiro 431) en Río Branco, Cerro Largo. (05/2018 - 05/2018 )

        Asociación de Cazadores del Uruguay 2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Charla sobre Hemoparásitos transmitidos por vectores en perros de caza, para miembros de la Asociación de Cazadores del Uruguay, veterinarios y público en general. 18 de mayo de 19.00 a 21.00 horas en la Casa de la Universidad de Cerro Largo (Dr. Luis A de Herrera 639) en Melo, Cerro Largo. (05/2018 - 05/2018 )

        Asociación de Cazadores del Uruguay 2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Charla sobre Hemoparásitos transmitidos por vectores en perros de caza, para miembros de la Asociación de Cazadores del Uruguay, veterinarios y público en general. 17 de mayo de 20.00 a 22.00 horas en la Sociedad Criolla los 33 (calle Agustín Fernández y ruta 17) en la ciudad de Treinta y Tres. (05/2018 - 05/2018 )

        Asociación de Cazadores del Uruguay 2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Charla sobre Fauna silvestre, garrapatas y enfermedades en Áreas Naturales Protegidas. (05/2018 - 05/2018 )

        Escuela Agraria de Melo ?Alcides F. Pintos?, Melo, Cerro Largo, Uruguay. Uruguay. 2 horas
        Areas de conocimiento:
        Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Charlas: Hemoparásitos transmitidos por vectores en perros en Uruguay. Breve reseña de la problemática de la garrapata común del ganado (Rhipicephalus microplus) en zonas libres del país. (04/2018 - 04/2018 )

        Centro Veterinario Colonia - COLAVECO ? Nueva Helvecia, Colonia, Uruguay. 2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Charla sobre Hemoparásitos en perros de caza: diagnóstico, tratamiento y control (01/2018 - 01/2018 )

        Para miembros de la Asociación de Controladores de Jabalí de Artigas y veterinarios., Sede de la Asociación Agropecuaria de Artigas
        2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • (08/2017 - 08/2017 )

        CENUR Litoral Norte – Salto, UdelaR, Salto, Laboratorio de Vectores y Enfermedades transmitidas
        10 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

      • Charla sobre Hemoparásitos transmitidos por garrapatas a caninos en Uruguay. (04/2017 - 04/2017 )

        INIA Tacuarembó, Tacuarembó, Uruguay 2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Charla sobre Hemoparásitos transmitidos por garrapatas a caninos en Uruguay. (04/2017 - 04/2017 )

        Centro Médico Veterinario de Melo, Cerro Largo, Uruguay. 2 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • (06/2015 - 06/2015 )

        CENUR Litoral Norte – Salto, UdelaR, Salto, Laboratorio de Vectores y Enfermedades transmitidas
        6 horas
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología

    • Otra actividad técnico-científica relevante

      • Participación de la reunión de la red CYTED (Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo). LaGar, número P117RT0292: Pautas de control químico y vacunal de las garrapatas del vacuno en América Latina (aprobada en la Convocatoria CYTED 2017), 02 al 06 de noviembre de 2019 en Querétaro, México. (11/2019 - 11/2019 )

        CYTED (Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo) 8 horas semanales
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Participación de la reunión anual de la red CYTED (Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo) LaGar, número P117RT0292: Pautas de control químico y vacunal de las garrapatas del vacuno en América Latina (aprobada en la Convocatoria CYTED 2017). 16 al 18 de octubre de 2018, en Ciudad de Panamá, Panamá. (10/2018 - 10/2018 )

        CYTED (Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo) . 8 horas semanales
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

    • Gestión Académica

      • Integrante de la Comisión de Dedicación Total del CENUR Litoral Norte. Resol. N° 7 del Consejo del CENUR Litoral Norte, fecha 28.03.19. Dist. N° 229/19 (Exp. 311170-000462-19). (03/2019 - a la fecha )

        Participación en consejos y comisiones 5 horas semanales

      • Coordinador del Laboratorio de Vectores y Enfermedades Transmitidas (07/2015 - a la fecha )

        CENUR Litoral Norte, Departamento de Ciencias Biológicas
        Gestión de la Investigación 40 horas semanales

      • Coordinador de Grupos Representantes de las Unidades de Investigación: Uruguay. Red CYTED (Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo) ?LaGar?, número P117RT0292: ?Pautas de control químico y vacunal de las garrapatas del vacuno en (01/2018 - a la fecha )

        Red CYTED (Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo) Otros 5 horas semanales
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Delegado alterno de Facultad de Veterinaria en la Mesa del Área de Tecnologías y Ciencias de la Naturaleza y del Hábitat del CENUR Litoral Norte. Resolución Consejo de la Facultad de Veterinaria del 14 de mayo de 2015 (Exp. N° 110011-001814-12). (05/2015 - a la fecha )

        Universidad de la República, Facultad de Veterinaria, CENUR Litoral Norte – Salto, UdelaR, Salto
        Participación en consejos y comisiones
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /

      • Representante (alterno) de la Mesa Coordinadora del Área de Tecnologías y Ciencias de la Naturaleza y el Hábitat ante el Consejo del CENUR Litoral Norte. Resolución de la Asamblea del Claustro del CENUR Litoral Norte adoptada en fecha 27.05.2020 (Exp. Nº (06/2020 - 05/2025 )

        Participación en cogobierno 3 horas semanales

      • Integrante del Comité de Ética de Investigación con Seres Humanos del CENUR Litoral Norte. Resol. Nº 3 - 14.12.17 del Consejo del Centro Universitario Regional Litoral Norte de la Universidad de la República. (12/2017 - 12/2024 )

        Participación en consejos y comisiones 2 horas semanales
        Areas de conocimiento:
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Ciencias de la Salud /

      • Integrante Suplente de la Asamblea del Claustro por el Orden Docente del CENUR Litoral Norte. (06/2018 - 07/2022 )

        Participación en cogobierno 1 horas semanales

      • Integrante Suplente de la Asamblea del Claustro por el Orden Docente del CENUR Litoral Norte. (05/2016 - 05/2018 )

        Universidad de la República, CENUR Litoral Norte
        Participación en cogobierno

      • Integrante TITULAR de la Asamblea del Claustro por el Orden Docente del Centro Universitario Regional Noroeste (05/2014 - 05/2016 )

        Universidad de la República, Regional Norte - Salto
        Participación en cogobierno

• Sector Educación Superior/Público - Programa de Desarrollo de las Ciencias Básicas - Uruguay

  Área Biología (PEDECIBA)

  • Vínculos con la Institución

    • Otro (02/2013 - a la fecha)

      Investigador Grado 4 PEDECIBA 5 horas semanales

    • Otro (09/2025 - 09/2025)

      Investigador Tutor de Micropasantía (año 2025) en el marco del ?Convenio de Cooperación existente en 2 horas semanales
      Temática de la Micropasantía: ¿Quién es Rangelia vitalii? Buscando su ADN en perros, zorros y garrapatas. Grupo inscripto: - Micropasantía 68. Docente responsable: María Cecilia Ferreira Ibarra, Estudiantes: Aylen Silva, Serena Spina Soria, Antonella Zucchi y Alejandro Marquesqui. Estudiantes del Liceo Departamental N.º 1 ?Instituto Politécnico Osimani y Llerena? ? Salto. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA), Ministerio de Educación y Cultura - Universidad de la República.

    • Otro (09/2024 - 10/2024)

      Investigador Tutor de Micropasantía (año 2024) en el marco del ?Convenio de Cooperación existente en 2 horas semanales
      Temática de la Micropasantía: ?Garrapatas, zorros y perros: detección de patógenos en laboratorio?. Grupo inscripto: - Micropasantía 54. Docente responsable: Mario Silva Francia, Estudiantes: Camila Basualdo López, Julieta Tessadri, Josefina Barboza, Abril Dutra. Estudiantes de 5º y/o 6º año de Bachillerato del Liceo Nº 2 de Salto (Departamento de Salto). Programa de Desarrollo de las Ciencias Básicas (PEDECIBA), Ministerio de Educación y Cultura - Universidad de la República. Constancia PG/225/24.

    • Otro (09/2023 - 09/2023)

      Docente de Micropasantía PEDECIBA-ANEP para grupos de 3 alumnos de 5º y 6º año de Bachillerato. 2 horas semanales
      Temática de la Micropasantía: ?Garrapatas, zorros y perros: detección de patógenos en laboratorio?. Grupo pasante: Alumnos pasantes: Tamara Peña, Ariadna Lequini y Sofía González. Docente responsable: Jhonny Sanchís. Liceo N °1, Ciudad: Salto, Departamento de Salto. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA), Ministerio de Educación y Cultura - Universidad de la República.

• Sector Gobierno/Público - Ministerio de Educación y Cultura - Uruguay

  Museo Nacional de Historia Natural

  • Vínculos con la Institución

    • Colaborador (01/2010 - a la fecha)

      Colaborador Honorario
      Entomología: Arácnidos. Malacología y Zoología invertebrados.

• Sector Enseñanza Técnico-Profesional/Secundaria/Público - Administración Nacional de Educación Pública - Uruguay

  Consejo de Formación en Educación / Centro Regional de Profesores del Litoral - Sede Salto

  • Vínculos con la Institución

    • Otro (07/2024 - 11/2024)

      Tutor investigador de dos estudiantes de cuarto año de la carrera de Profesorado, especialidad Cienc 4 horas semanales
      En el marco de la instancia curricular "Tutoría de Pasantía" (de carácter obligatorio) a cargo de la Prof. Moriana Rebuffo, de la malla curricular de la carrera de Profesorado, especialidad Ciencias Biológicas, en el Sistema Único Nacional de Formación Docente, Plan 2008. Dirección del Centro Regional de Profesores del litoral - Sede Salto. Consejo de Formación en Educación, Administración Nacional de Educación Pública (ANEP), Uruguay.

    • Otro (07/2023 - 11/2023)

      Tutor investigador de estudiantes de cuarto año de la carrera de Profesorado, especialidad Ciencias. 4 horas semanales
      Sistema Único Nacional de Formación Docente, Plan 2008 del Centro Regional de Profesores del Litoral - Sede Salto en el marco de la instancia curricular "Tutoría de Pasantía" (de carácter obligatorio) a cargo de la Prof. Moriana Rebuffo.

• Sector Extranjero/Internacional/Otros - Argentina

  Sociedad Latinoamericana de Acarología

  • Vínculos con la Institución

    • Otro (01/2016 - 01/2024)

      Vocal de Uruguay de la Junta Directiva 2 horas semanales
      - Vocal de Uruguay de la Junta Directiva de la Sociedad Latinoamericana de Acarología (Período 2016-2020). - Vocal de Uruguay de la Junta Directiva de la Sociedad Latinoamericana de Acarología (Período 2020-2024).

  • Actividades

    • Docencia

      • Curso HABLANDO DE ÁCAROS: Tópicos de actualización en Acarología Agrícola, Médico Veterinaria, y Biodiversidad (07/2021 - 09/2021 )

        Perfeccionamiento
        Organizador/Coordinador
        Areas de conocimiento:
        Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Acarología
        Ciencias Agrícolas / Agricultura, Silvicultura y Pesca / Agricultura / Acarología
        Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología / Acarología

      • Curso HABLANDO DE ÁCAROS: Tópicos de actualización en Acarología Agrícola, Médico Veterinaria, y Biodiversidad (05/2020 - 07/2020 )

        Perfeccionamiento
        Invitado

Carga horaria

• Carga horaria de docencia: 15 horas
  
• Carga horaria de investigación: 10 horas
  
• Carga horaria de formación RRHH: 8 horas
  
• Carga horaria de extensión: 2 horas
  
• Carga horaria de gestión: 5 horas
  

Producción científica/tecnológica

• La principal área de investigación es sobre sistemática de garrapatas y las enfermedades que éstas transmiten. Esta línea comenzó con la colecta y clasificación de especies existentes en Uruguay, tras lo cual se realizó una revisión para nuestro país. En conjunto con investigadores de países vecinos se realizaron trabajos de sistemática sobre garrapatas de la región. Esta cooperación produjo en los últimos años abundante material científico para Uruguay, Chile, Argentina y Brasil (ver publicaciones internacionales arbitradas), entre las que se destacan la descripción de varias nuevas especies de garrapatas para la Región Neotropical, incluida la descripción de Ornithodoros rioplatensis basada en material de Uruguay. Posteriormente se comenzaron los estudios de las enfermedades transmitidas por garrapatas con especial énfasis a humanos. Las garrapatas luego de los mosquitos son los vectores más importantes de enfermedades para animales y humanos. Ante tanta diversidad de agentes patógenos transmitidos es de esperar que en nuestro país existiesen otras enfermedades transmitidas por garrapatas, que no sean la babesiosis y anaplasmosis bovina o la rickettsiosis humana diagnosticada como "fiebre botonosa". En primer lugar se plateó el estudio de cuáles eran las principales especies de garrapatas que parasitaban a humanos en nuestro país y si estaban relacionadas geográficamente con los casos de rickettsiosis. El diagnóstico de esta enfermedad en Uruguay en la década de los 90´ se realizó a través de inmunofluorescencia indirecta (IFI) y el agente incriminado fue Rickettsia conorii, e inicialmente el vector fue clasificado como Amblyomma maculatum. Estudios sistemáticos del vector nos permitieron establecer que Amblyomma maculatum no existe en Uruguay y que ha sido confundida con Amblyomma triste, la principal garrapata que parasita a humanos en nuestro país. El agente de la rickettsiosis, diagnosticado primariamente por IFI, Rickettsia conorii, en realidad sólo se registra en el “Viejo Mundo” y es casi exclusivamente transmitido por la garrapata Rhipicephalus sanguineus y no por el género Amblyomma, cuyas especies son importantes vectores de enfermedades para humanos en nuestro continente. Mediante estudios epidemiológicos y de detección de patógenos por técnicas moleculares para evitar las posibles reacciones cruzadas, que se producen con las técnicas serológicas como la IFI, demostramos que el agente patógeno circulante en áreas consideradas endémicas de Uruguay es Rickettsia parkeri y no R. conorii. Y que el vector implicado en los casos se trata de A. triste.
  Más recientemente se han incluido dos líneas nuevas de investigación, una sobre las principales parasitosis en peces de la región de la represa de Salto Grande que puedan repercutir en la salud de los peces u ocasionar zoonosis parasitarias humanas, dicha línea está generando una importante cantidad de información. Y la restante es sobre las trematodosis que afectan a bovinos de establecimiento de leche y carne en la zona norte del país, cuyos resultados preliminares nos ha permitido saber sobre el desconocimiento existente sobre estas parasitosis en los productores.
  

Producción bibliográfica

• Artículos publicados

  • Arbitrados

    • A novel ecotype of Anaplasma phagocytophilum complex in questing Ixodes fuscipes ticks. (Completo, 2026)

      Félix M.L , Santodomingo, A. , Thomas, R. , QUEIROLO, D. , Muñoz-Leal, S. , VENZAL, J.M.
      Parasites & Vectors, v.: 19 1 , p.:96 - 96, 2026
      Palabras clave: Anaplasma phagocytophilum Ixodes fuscipes Ecotypes Tick-borne diseases Uruguay.
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / NO CORRESPONDE / Parasitología
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Medio de divulgación: Internet
      E-ISSN: 17563305
      DOI: https://doi.org/10.1186/s13071-025-07226-8
      
      

    • A novel genotype of Babesia microti-like group in Ixodes montoyanus ticks parasitizing the Andean bear (Tremarctos ornatus) in Ecuador (Completo, 2025)

      Santodomingo, A. , Enríquez, S. , Thomas, R. , Muñoz-Leal, S. , FÉLIX, M.L. , Castellanos, A. , Bermúdez, S. , VENZAL, J.M.
      Experimental and Applied Acarology, v.: 94 2 , p.:30 - 30, 2025
      Palabras clave: South America Spectacled Bear Ursidae Tick-borne diseases Piroplasmids Sanger sequencing
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Internet
      ISSN: 01688162
      E-ISSN: 15729702
      DOI: https://doi.org/10.1007/s10493-024-00990-9
      Babesia species (Piroplasmida) are hemoparasites that infect erythrocytes of mammals and birds and are mainly transmitted by hard ticks (Acari: Ixodidae). These hemoparasites are known to be the second most common parasites infecting mammals, after trypanosomes, and some species may cause malaria-like disease in humans. Diagnosis and understanding of Babesia diversity increasingly rely on genetic data obtained through molecular tech¬niques. Among hard ticks, several Ixodes species are known vectors of Babesia microti-like species in the Northern Hemisphere. Recently, Ixodes and Amblyomma ticks have been recorded parasitizing the Andean bear (Tremarctos ornatus) in Ecuador. Previous reports have suggested babesiosis in a fatal case of this threatened bear species in that country. This study aimed to detect Piroplasmida DNA in hard ticks collected from Ande¬an bears at two sites in Ecuador. This species plays a critical role as an ecological engineer and a seed disperses, contributing significantly to the maintenance and health of Andean ecosystems. Twelve ticks screened with conventional PCR and Piroplasmida DNA was amplified from one Ixodes montoyanus tick collected from a free-living female Andean bear at Llanganates National Park. Two Babesia sequences were characterized: one for the 18 S ribosomal rRNA gene and another for the cytochrome c oxidase 1 gene. Phylogenetic analyses for both loci placed these sequences within the B. microti-like clade. This study reports a novel B. microti-like genotype identified in an I. montoyanus parasitizing a fe¬male Andean bear, contributing to the knowledge of the diversity of this group in South America. Given their conservation status, future epidemiological surveillance of Babesia and other tick-borne infectious agents in Andean bears is needed.
      
      

    • A survey for piroplasmids in questing Ixodes fuscipes ticks reveals undescribed Babesia lineages in Uruguay (Completo, 2025)

      RODRIGO ALVEZ , Félix, M.L. , ADRIANA SANTODOMINGO , PABLO PARODI , RICHARD THOMAS , SEBASTIÁN MUÑOZ-LEAL , LUIS CARVALHO , JOSÉ M. VENZAL
      Parasites & Vectors, v.: 18 p.:225 - 225, 2025
      Palabras clave: Vector-borne piroplasmids Babesia spp. Ixodes fuscipes One Health Uruguay
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / NO CORRESPONDE / Parasitología
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Medio de divulgación: Internet
      Lugar de publicación: United kingdom
      E-ISSN: 17563305
      DOI: 10.1186/s13071-025-06866-0
      https://doi.org/10.1186/s13071-025-06866-0
      Background Ixodes fuscipes is a tick species found in the Southern Cone of America and the only member of the Ixodes ricinus complex present in Uruguay. Members of this complex are particularly recognized as vectors of diseases affecting human health, such as babesiosis, caused by parasites of the genus Babesia (Apicomplexa: Piroplasmida). However, even though potential hosts of I. fuscipes in Uruguay (rodents, birds, and artiodactyls) are known carriers of Babesia species, the potential role of I. fuscipes as a vector of piroplasmids has not been studied. Methods In this study, questing I. fuscipes ticks were collected from five locations in Uruguay, and the presence of piroplasmid DNA was assessed using polymerase chain reaction (PCR) to amplify fragments of the small subunit ribosomal RNA (18S rRNA) and cytochrome c oxidase subunit 1 (COI) genes. Results A total of 953 ticks (larvae, nymphs, and adults) were collected; 14 samples (two larval pools and 12 nymphs) tested positive. Genetic analyses using 18S rDNA and COI sequences revealed the presence of undescribed Babesia lineages, belonging to the Babesia odocoilei clade and others to the Babesia microti sensu stricto clade. Conclusions This work represents the first association of Babesia spp. with I. fuscipes and highlights the importance of this type of study to detect and mitigate the emergence of diseases associated with these arthropods.
      
      

    • The Ixodes auritulus complex (Acari: Parasitiformes: Ixodidae) in the Southern Cone of America (Completo, 2025)

      JOSÉ M. VENZAL , EVELINA L. TARRAGONA , FERNANDO S. FLORES , FÉLIX, M.L. , GABRIEL L. CICUTTIN , PATRICK S. SEBASTIAN , MARCELO B. LABRUNA , ALBERTO A. GUGLIELMONE , SANTIAGO NAVA
      Systematic Parasitology, v.: 102 p.:35 - 35, 2025
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      Lugar de publicación: Netherlands
      ISSN: 01655752
      E-ISSN: 15735192
      DOI: 10.1007/s11230-025-10230-2
      https://doi.org/10.1007/s11230-025-10230-2
      Ixodes auritulus Neumann, 1904 (Acari: Parasitiformes: Ixodidae) represents a species complex principally associated to birds belonging to the orders Ciconiiformes, Charadriiformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Piciformes, Pelecaniformes, Procellariiformes, Strigiformes, and Tinamiformes in both immature and adult stages. This is a cosmopolitan tick species whose distribution encompass the Afrotropical, Australasian, Nearctic and Neotropical Zoogeographic Regions, and Pacific Oceans islands. Ixodes auritulus sensu stricto was described from southern Chile, and recently new species from this complex were described based only on morphological characters. In this study, specimens of ticks determined to belong to the I. auritulus complex obtained from the Southern Cone of America in different biogeographic regions of Argentina, southern Chile, Brazil and Uruguay were analyzed. Additionally, a female paratype of Ixodes rio Apanaskevich & Labruna, 2022 from the southern from Brazil was included in the study. Morphological characters were analyzed and phylogenetic analyses were performed by obtaining partial mitochondrial DNA sequences of the 16S rRNA and cox1 genes. The specimens from Punta Arenas, Magallanes Province, southern Chile (type locality) correspond morphologically to I. auritulus s.s. and those from central and northern Argentina (Pampa and Yungas Biogeographic Provinces), Uruguay and southern Brazil (Pampa Biogeographic Province) were morphologically compatible with I. rio. The phylogenetic analysis based on mitochondrial DNA sequences support the classification of I. auritulus s.s. from southern Chile and I. rio from Argentina, Brazil and Uruguay as two distinct species. Additional morphological and molecular analyses of ticks from ecological regions other than those included here are necessary to deepen the knowledge of the diversity of the I. auritulus complex in the Southern Cone of America.
      
      

    • Are Babesia vogeli genotypes associated with Rhipicephalus sanguineus and Rhipicephalus linnaei distribution? (Completo, 2025)

      VINÍCIUS BAGGIO-SOUZA , LAURA BERGER , RAFAELA MALLMANN-BOHN , ADEYLDES OLIVEIRA REIS , LAY GRECO BASILIO , YASMIN SANTANNA PEREIRA , LUIZA TIRELLI , RENATA FAGUNDES-MOREIRA , JOÃO VITOR DOS SANTOS ALVES DA SILVA , LORENA FREITAS DAS NEVES , LUDMILA RODRIGUES MOROZ , HUARRISSON AZEVEDO SANTOS , MARIA E. MARECOS ESPINOZA , CÍNTIA DAUDT , FABIO GREGORI , ALINE GIROTO-SOARES , FLÁVIO ROBERTO CHAVES DA SILVA , JOÃO LUIS GARCIA , DANIEL MOURA DE AGUIAR , MARISTELA PECKLE PEIXOTO , JOSÉ MANUEL VENZAL , FILIPE DANTAS-TORRES , MARCOS ROGÉRIO ANDRÉ , JOÃO FABIO SOARES
      Parasites & Vectors, v.: 18 p.:282 - 282, 2025
      Palabras clave: Canine babesiosis Tick-borne diseases Brown dog ticks
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / NO CORRESPONDE / Parasitología
      Medio de divulgación: Internet
      Lugar de publicación: United kingdom
      E-ISSN: 17563305
      DOI: 10.1186/s13071-025-06923-8
      https://doi.org/10.1186/s13071-025-06923-8
      Background In South America, Babesia vogeli is the primary causative agent of canine babesiosis, and brown dog ticks (Rhipicephalus sanguineus sensu lato) are the vectors. The recent separation of brown dog ticks into Rhipicephalus sanguineus sensu stricto (?temperate lineage?) and Rhipicephalus linnaei (?tropical lineage?) raised suspicions of the possibility of two distinct Babesia genotypes or even species being transmitted by these tick species. Methods To investigate this hypothesis, dog blood samples from Brazil (eight states), Paraguay, and Uruguay were collected to determine the genetic diversity of B. vogeli in South America. The samples were collected from temperate regions (southern Brazil and Uruguay), where the putative vector is R. sanguineus, and from the tropical areas (southeastern, midwestern, northeastern, and northern Brazil and Paraguay), where R. linnaei is the vector. DNA samples from B. vogeli-positive dogs were extracted to amplify the 18S ribosomal RNA, internal transcribed spacers 1 and 2, heat shock protein 70, cytochrome c oxidase subunit 1, cytochrome oxidase c subunit 3, and cytochrome b genes. The sequences obtained were aligned with available B. vogeli sequences in GenBank and other homologous sequences to construct phylogenetic trees, haplotype networks, and matrices. Results Our haplotypic and phylogenetic analyses congruently indicated the existence of one genotype in temperate areas and another in tropical areas, where R. sanguineus and R. linnaei act as vectors, respectively. While the percentage of similarity varied among the evaluated genetic markers, the results indicated a clear differentiation between the B. vogeli genotypes associated with temperate and tropical regions. Conclusions Our data indicate the existence of two B. vogeli genotypes in South America, associated with temperate and tropical areas. This contributes to a better understanding of B. vogeli?s genetic diversity and opens new avenues for researching the ecology and coevolution of B. vogeli genotypes and their tick vectors. Owing to their correlation with the climatic region and the historical nomenclature of their vectors, we suggest the nomenclature of ?temperate?and ?tropical? B. vogeli genotypes.
      
      

    • Spotted fever group Rickettsia and Babesia odocoilei in hard ticks (Acari: Ixodidae) from wild areas in Panama (Completo, 2025)

      SERGIO BERMUDEZ CASTILLERO , MARÍA FÉLIX , LILLIAN DOMINGUEZ , JOSÉ VENZAL
      Acarological Studies, v.: 7 p.:101 - 110, 2025
      Palabras clave: Ixodidae Rickettsia spp. Babesia odocoilei wild environments Panama
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 26675684
      DOI: 10.47121/acarolstud.1726467
      https://doi.org/10.47121/acarolstud.1726467
      This study evaluated the presence of Rickettsia, Anaplasmataceae, Borrelia and Piroplasmida in Amblyomma and Ixodes spp. ticks from wild areas of Panama. Molecular detection of pathogens was performed on the following species of ticks: Amblyomma dissimile, Amblyomma mixtum, Amblyomma naponense, Amblyomma cf. oblongoguttatum, Amblyomma ovale, Amblyomma tapirellum, Haemaphysalis juxtakochi, Ixodes affinis, and Ixodes sp. nymphs. Partial sequences of Ixodes nymphs with the 16S rRNA gene showed low similarity to other Ixodes species. We found evidence of DNA from Rickettsia spp. in A. mixtum (R. amblyommatis), A. dissimile (?Candidatus R. colombianensi?), I. affinis (Rickettsia sp.), and Babesia odocolei in I. affinis. No evidence of DNA from the target microorganisms was detected in A. naponense, A. cf. oblongogutta-tum, A. ovale, A. tapirellum, H. juxtakochi, or Ixodes sp. This is the first report of B. odocolei in I. affinis, which represents an additional risk of zoonosis. The detection of Rickettsia spp. corroborates previous findings in Panama. Finally, this study was crucial to determine a putative new species of Ixodes, from two nymph feeding on human.
      
      

    • New records of soft ticks (Acari: Argasidae) from caves in Brazil, with a morphological study of Ornithodoros fonsecai and an analysis of the taxonomic status of Antricola inexpectata (Completo, 2024)

      Oliveira, G.M.B. de , Muñoz-Leal, S. , Nava, S. , Horta, M.C. , Bernardi, L. , VENZAL, J.M. , Marcelo B. Labruna
      Ticks and Tick-borne Diseases, v.: 15 3 , p.:10233 - 10233, 2024
      Palabras clave: Argasids Ornithodoros cavernicolous Antricola guglielmonei Morphometry Principal component analysis Caatinga
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 18779603
      DOI: doi: 10.1016/j.ttbdis.2024.102331
      In this study, we report soft ticks from bat-inhabiting caves in different areas of Brazil. From 2010 to 2019, we collected 807 tick specimens from nine caves located in four Brazilian states among two biomes. Ticks were morphologically identified as Antricola guglielmonei (282 specimens), Ornithodoros cavernicolous (260 specimens), and Ornithodoros fonsecai (265 specimens). Whereas A. guglielmonei was collected on bat guano in hot caves, O. cavernicolous and O. fonsecai were collected in cracks and crevices on the walls of cold caves, sometimes in the same chamber. Morphological identifications were corroborated by molecular and phylogenetic analyses inferred from tick mitochondrial 16S rRNA gene partial sequences. The sequences of A. guglielmonei, O. cavernicolous and O. fonsecai collected in this study clustered with conspecific GenBank sequences from different localities of Brazil. Remarkably, a clade containing 12 sequences of O. fonsecai was clearly bifurcated, denoting a degree of genetic divergence (up to 5 %) of specimens from Cerrado/Atlantic Forest biomes with the specimens from the Caatinga biome. To further evaluate this divergence, we performed morphometric analysis of the larval stage of different O. fonsencai populations by principal component analysis, which indicated that the larvae from Caatinga populations were generally smaller than the larvae from other biomes. Some of the present A. guglielmonei specimens were collected from the type locality of Antricola inexpectata. Comparisons of these specimens with the type specimens of A. inexpectata and A. guglielmonei indicated that they could not be separated by their external morphology. Hence, we are relegating A. inexpectata to a synonym of A. guglielmonei. This proposal is corroborated by our phylogenetic analysis.
      
      

    • Molecular screening of tick-borne microorganisms in ticks from rural areas of Panama, with the first record of Ehrlichia minasensis in Rhipicephalus microplus from Central America. (Completo, 2024)

      Bermúdez C., S.E. , FÉLIX, M.L. , Domínguez, A.L. , Araúz, D. , VENZAL, J.M.
      Veterinary Research Communications, v.: 48 p.:1301 - 1308, 2024
      Palabras clave: Rural environments Rickettsia Anaplasmataceae Central America
      Medio de divulgación: Internet
      Lugar de publicación: 10.1007/s11259-024-10306-2
      ISSN: 01657380
      E-ISSN: 15737446
      During a molecular screening of rickettsial, borrelial and protozoal agents in ticks from rural areas of Panama, we detected DNA of Rickettsia amblyommatis in four Amblyomma mixtum collected from horses in a locality in Los Santos province. Ehrlichia minasensis and Anaplasma marginale were identified from three and two Rhipicephalus microplus, respectively, collected on cows from Chiriqui province. No DNA from other tick-borne agents was found. These results increase the information of R. amblyommatis and A. marginale in Panama, and also mark the first record of E. minasensis in Central America.
      
      

    • Molecular survey of Piroplasmida, Hepatozoon spp. and Anaplasmataceae in anemic and thrombocytopenic dogs from Uruguay (Completo, 2024)

      Parodi, P , Bazzano, V. , ARMUA-FERNANDEZ, M. T.; ARMUA, M.T. , FÉLIX, M.L. , CARVALHO L , Freire, J. , VENZAL, J.M.
      Veterinary Parasitology. Regional Studies and Reports, v.: 51 p.:1 - 8, 2024
      Palabras clave: Tick-borne diseases Canine hemoparasites anemia Thrombocytopenia Uruguay
      Medio de divulgación: Internet
      E-ISSN: 24059390
      DOI: doi: https://doi.org/10.1016/j.vprsr.2024.101027
      Canine tick-borne diseases, such as babesiosis, rangeliosis, hepatozoonosis, anaplasmosis and ehrlichiosis, are of veterinarian relevance, causing mild or severe clinical cases that can lead to the death of the dog. The aim of this study was detecting tick-borne protozoan and rickettsial infections in dogs with anemia and/or thrombocytopenia in Uruguay. A total of 803 domestic dogs were evaluated, and 10% were found positive (detected by PCR) at least for one hemoparasite. Sequence analysis confirmed the presence of four hemoprotozoan species: Rangelia vitalii, Babesia vogeli, Hepatozoon canis and Hepatozoon americanum, and the rickettsial Anaplasma platys. The most detected hemoparasite was R. vitalii, followed by H. canis and A. platys. This is the first report of B. vogeli in Uruguay and the second report of H. americanum in dogs from South America. The results highlight the importance for veterinarians to include hemoparasitic diseases in their differential diagnosis of agents causing anemia and thrombocytopenia.
      
      

    • Molecular detection of rickettsial agents in Amblyomma maculatum ticks (Ixodida: Ixodidae) from Ecuador (Completo, 2024)

      Enríquez, S. , FÉLIX, M.L. , Bermúdez, S. , Mosquera, V. , Farovitch, L. , Vaca, F. , Guevara, J.M. , VENZAL, J.M.
      Veterinary Research Communications, v.: 48 p.:2869 - 2876, 2024
      Palabras clave: Ehrlichia Human pathogen Rickettsia Tick-borne diseases
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Medio de divulgación: Internet
      E-ISSN: 15737446
      DOI: https://doi.org/10.1007/s11259-024-10410-3
      Rickettsiales are obligate intracellular bacteria that need vertebrates and arthropods to maintain their life cycles. Some species of the genera Anaplasma, Ehrlichia, and Rickettsia are transmitted by ticks to both animals and humans and can cause mild to severe and even fatal cases. In the Americas, there is substantial data on rickettsial agents, encompassing both clinical cases and the detection of these agents in ticks, but in Ecuador, the information about them remains poorly understood. Therefore, the objective of this study was to detect molecularly rickettsial agents in Amblyomma maculatum ticks in both parasitic and free-living phases collected from domestic animals and pasture in five localities across three coastal provinces of Ecuador. Rickettsia parkeri, Candidatus Rickettsia andeanae, and Ehrlichia sp. were recorded in A. maculatum for the first time in Ecuador. These records were made in a region where antibodies to the Spotted Fever Rickettsia Group were detected in humans. Additional studies are needed to characterize Ehrlichia sp. at a specific level. Furthermore, recognizing the specific Rickettsiales species circulating in the ticks and the hosts within a region is crucial for assessing potential contact risks.
      
      

    • Ticks (Acari: Ixodidae, Argasidae) associated with wild birds in Argentina (Completo, 2023)

      Flores, F.S. , Saracho-Bottero, M.N. , Tarragona, E.L. , Sebastian, P.S. , Copa, G.N. , Guardia, L. , Mangold, A.J. , VENZAL, J.M. , Nava, S.
      Ticks and Tick-borne Diseases, v.: 14 3 10213, p.:1 - 11, 2023
      Palabras clave: Ticks Birds Ixodidae Argasidae Phytogeographic Provinces Argentina
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: https://doi.org/10.1016/j.ttbdis.2023.102135
      The aim of this study was to report tick infestations on wild birds from four Phytogeographic Provinces of Argentina. A total of 1085 birds was captured (124 species, 97 genera, 29 families and 13 orders), and ticks were collected from 265 birds (48 species, 40 genera and five orders). A total of 1469 ticks (1102 larvae, 363 nymphs and 4 females) belonging to 15 tick species (Amblyomma calcaratum, Amblyomma dubitatum, Amblyomma nodosum, Amblyomma ovale, Amblyomma parvum, Amblyomma sculptum, Amblyomma tigrinum, Amblyomma triste, Haemaphysalis juxtakochi, Haemaphysalis leporispalustris, Ixodes auritulus sensu lato, Ixodes pararicinus, Ixodes silvanus, Ixodes sp. cf. I. affinis and Ornithodoros sp. cf. O. mimon). Eighty-one new associations between bird species and stages of tick species are detected. The families Thamnophilidae, Turdidae, Thraupidae, Passerellidae, Furnariidae and Troglodytidae were the most prevalent. According to the Phytogeographic Provinces involved in this study, the prevalence of infection for each of them in birds was: (1) Chaco: 28.2% (11 tick species); (2) Yungas: 22.0% (8 tick species); (3) Espinal: 11.1% (2 tick species); and (4) Pampa: 3.9% (1 tick species). This study provided information on the diversity of tick species that parasitize wild birds, the variability of the specific tick-bird associations between the different Phytogeographic Provinces and the relevance of some families of birds as hosts of different tick species.
      
      

    • Analysis of the tick communities associated to domestic mammals in rural areas of the Yungas montane forest from Argentina (Completo, 2023)

      Copa, G.N. , Flores, F.S. , Tarragona, E.L. , Lamattina, D. , Sebastian, P.S. , Gil, J.F. , Mangold, A.J. , VENZAL, J.M. , Nava, S.
      Veterinary Parasitology. Regional Studies and Reports, v.: 39 10085, p.:1 - 9, 2023
      Palabras clave: Ixodidae Yungas domestic mammals rural areas tick-borne pathogens Argentina
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 24059390
      DOI: https://doi.org/10.1016/j.vprsr.2023.100850
      The aim of this work was to describe the tick community associated to domestic mammals in rural areas from the Yungas lower montane forest of Argentina. The circulation of tick-borne pathogens was also analyzed. Samples of ticks parasitizing cattle, horses, sheep and dogs were carried out in different seasons, and questing ticks were collected from vegetation and analyzed to detect the presence of Rickettsia, Ehrlichia, Borrelia and Babesia by a battery of different PCRs. The structure of the tick communities was analyzed through the Chao1 species richness estimator, the Shannon?Wiener index and the Horn index of community similarity. Eight tick species were collected in the study area: Amblyomma sculptum, Rhipicephalus microplus, Amblyomma hadanii, Dermacentor nitens, Amblyomma ovale, Haemaphysalis juxtakochi, Ixodes pararicinus and Rhipicephalus sanguineus sensu stricto. However, A. sculptum was by far the dominant species in the tick assemblages analyzed, and this was reflected in the low diversity values obtained. Dermacentor nitens, A. sculptum and R. microplus were the three species associated to horses. The predominance of A. sculptum was also observed in the tick samples obtained from dogs, even on two tick species, namely A. ovale and R. sanguineus s.s., which have dogs as the principal domestic host. Rhipicephalus microplus and A. sculptum were the most abundant ticks on cattle, while few specimens of I. pararicinus, A. hadanii and D. nitens were found on bovines. Dermacentor nitens ticks were found to be infected with B. caballi, which indicate the circulation of this pathogen of horses in the Yungas area. The detection of a strain of Borrelia sp. belonging to the B. burgdorferi s.l. complex in I. pararicinus is consistent with previous findings made in Argentina, but the public health relevance of this vector-microorganism association is far from being similar to that occurs in the northern hemisphere because there are practically no records of these tick species parasitizing humans in South America. The tick community of rural areas of the Yungas lower montane forest is composed by species which are potential vectors of pathogenic microorganism with veterinary and public health importance, circulating in a human-wildlife-livestock interface.
      
      

    • Morphological and molecular identification of the hard ticks parasitizing Tremarctos ornatus (Carnivora: Ursidae) from paramo of Ecuador. (Completo, 2023)

      Enríquez, S. , FÉLIX, M.L. , Castellanos, A. , Bermúdez, S. , VENZAL, J.M.
      Acarological Studies, v.: 5 2 , p.:45 - 51, 2023
      Palabras clave: Ixodidae Amblyomma Ixodes Andean bear paramo Ecuador
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Internet
      E-ISSN: 26675684
      DOI: 10.47121/acarolstud.1261833
      https://dergipark.org.tr/en/pub/acarolstud
      The Andean bear or spectacled bear, Tremarctos ornatus (Cuvier), inhabits the Andes and is considered an endangered species due to anthropogenic factors. The aim of this study was to identify the tick species parasitizing the Andean bears in the evergreen shrubland and paramo grassland ecosystem in the Andes Mountain Range of Ecuador. Twenty-six ticks were removed from five Andean bears and morphologically identified as Amblyomma multipunctum Neumann, Ixodes boliviensis Neumann and Ixodes montoyanus Cooley. One specimen of each species was also molecularly analyzed and confirmed by BLAST. This study confirms the presence of I. boliviensis parasitizing T. ornatus and adds new records of A. multipunctum and I. montoyanus in its distribution. The parasite-host relationships are new in all cases for Ecuador.
      
      

    • Description of a new Pavlovskyella species (Acari: Argasidae) from Chile. (Completo, 2023)

      Muñoz-Leal,S. , VENZAL, J.M. , Kneubehl, A.R. , Lopez, J.E. , Martins, T.F. , Labruna, M.B.
      Journal of Medical Entomology, v.: 60 5 , p.:968 - 977, 2023
      Palabras clave: Argasidae soft tick mitogenome phylogeny new species
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 19382928
      DOI: https://doi.org/10.1093/jme/tjad091
      Soft ticks (Argasidae) of the Pavlovskyella Pospelova-Shtrom subgenus are important vectors of relapsing fever spirochetes, which are agents of disease globally. South American representatives of the Pavlovskyella subgenus include 3 species: Ornithodoros (Pavlovskyella) brasiliensis Aragão, Ornithodoros (Pavlovskyella) furcosus Neumann, and Ornithodoros (Pavlovskyella) rostratus Aragão. Here, we describe a fourth species based on morphological and mitogenomic evidence of ticks collected in burrows of unknown hosts in central Chile. The larva of the new species separates from other South American soft ticks by the following combination of characters: 13 pairs of dorsolateral setae, dorsal plate hexagonal, hypostome blunt with denticles from apex almost to the base. Adults of this new species lack cheeks, possess a dorsoventral groove, and have humps, similar to O. (P.) brasiliensis; however, they lack bulging structures on the flanks of idiosoma. Moreover, females and males differ from O. (P.) rostratus by having 3 humps instead of spurs in tarsi I and from O. (P.) furcosus because of their smaller size and thinner anterior lip of the genital aperture in females. The phylogenetic analysis performed with mitogenomes of the Argasidae family depicts the new Pavlovskyella species from Chile in a monophyletic clade with other South American species in the subgenus, confirming a regional group.
      
      

    • A novel Babesia sp. of the "Western Babesia group", detected in opossums from Guatemala. (Completo, 2023)

      Thomas, R. , Santodomingo, A. , Parragué-Migone, C. , Portillo, E. , Barrios, M. , VENZAL, J.M. , Muñoz-Leal, S.
      Ticks and Tick-borne Diseases, v.: 14 6 , p.:1 - 5, 2023
      Palabras clave: Amblyomma Didelphis marsupialis Marsupials Ornithodoros Piroplasmida
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 18779603
      DOI: doi: 10.1016/j.ttbdis.2023.102248
      Babesia spp. are tick-borne protozoans that involve birds and mammals in their transmission cycles and cause babesiosis, a severe hemolytic malaria-like disease. Opossums of the genus Didelphis are recognized hosts of tick-borne pathogens. Therefore, exploring tick-borne agents in Didelphis species is important to understand the circulation of pathogens in areas where opossums occur. In this study, we targeted Anaplasmataceae, Babesia, Borrelia and Hepatozoon DNA in ticks, blood and organ samples collected from three hunted Didelphis marsupialis specimens in eastern Guatemala. While the samples were negative for Hepatozoon and bacterial DNA, sequences of Babesia 18S rDNA, cox1 and cytb genes were retrieved from two opossums. Ticks collected on the animals included Amblyomma parvum and an undetermined Ornithodoros sp. The Babesia sp. detected in this study (Babesia sp. THB1-2) clusters phylogenetically within the "Western Babesia group", which includes pathogenic species such as Babesia conradae, Babesia duncani, and Babesia negevi. Our results represent the first record of a Babesia sp. in Guatemala and highlight the importance of D. marsupialis as potential spreaders of ticks and pathogens in Central America.
      
      

    • Novel Anaplasma (Rickettsiales: Anaplasmataceae) strain and Hepatozoon sp. cf. H. procyonis (Apicomplexa, Hepatozoidae) detected in Procyon cancrivorus (Carnivora, Procyonidae) from Argentina, with note of tick-host association. (Completo, 2023)

      Tarragona, E.L. , Sebastian, P.S. , FÉLIX, M.L. , VENZAL, J.M.
      Veterinary Research Communications, v.: 47 4 , p.:2241 - 2245, 2023
      Palabras clave: Anaplasma Hepatozoon Amblyomma ovale Procyon cancrivorus Argentina
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Internet
      E-ISSN: 15737446
      DOI: https://doi.org/10.1007/s11259-023-10099-w
      The aim of this work is to report the first detection of Procyon cancrivorus naturally co-infected with Hepatozoon sp. cf. H. procyonis and a novel Anaplasma strain from South America and potential vector tick species associated. On August 30, 2016, a specimen of P. cancrivorus was found dead on the route in Chaco province, Argentina. A tick and a blood sample by cardiac puncture was collected from the specimen. DNA was extracted from blood sample and the tick was morphological identity as a female of Amblyomma ovale. Molecular detection of Anaplasmataceae family and Hepato¬zoon spp. agents was performed targeting two different loci: 16 S rRNA and 18 S rRNA gene. The phylogenetic analyses show that the Anaplasma sp. strain detected in P. cancrivorus in this study is similar to Anaplasma sp. strains previously detected in Nasua nasua and A. ovale from Brazil. Furthermore, Hepatozoon sp. of the H. procyonis group was amplified that is phylogenetically closely related to H. procyonis reported in N. nasua from Brazil. Since it was not exactly the same as the latter, it was decided to name at Hepatozoon sp. cf. H. procyonis. It is possible that, this potential new species of Anaplasma would be specific for Procyonidae family and there are two species of Hepatozoon linked to this family in South America. These results added to other published studies suggest that A. ovale could be a potential vector both for the new potential strain of Anaplasma and for the Hepatazoon sp. of the H. procyonis group.
      
      

    • Description of two new species in the Ixodes ricinus complex from the New World (Acari: Ixodidae), and redescription of Ixodes affinis Neumann, 1899 (Completo, 2023)

      Nava, S. , Beati, L , VENZAL, J.M. , Durden, L.A. , Bermudez, S.E. , Tarragona, E.L. , Mangold, A.J. , Gleason, D. , Mastropaolo, M. , Guglielmone, A.A.
      Zootaxa, v.: 5361 1 , p.:53 - 73, 2023
      Palabras clave: Ixodes taxonomy New World new species
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Internet
      E-ISSN: 11755334
      DOI: https://doi.org/10.11646/zootaxa.5361.1.2
      Ixodes chacoensis n. sp. is described based on males, females, nymphs and larvae collected from vegetation, ungulates and passerine birds in northeastern Argentina. Ixodes affinis Neumann, 1899 is redescribed based on the original type specimens (females) from Leopardus pardalis, and from recently collected specimens from Costa Rica. Ixodes keiransi n. sp., previously treated as North American populations of Ixodes affinis, is described based on males and females from carnivores and ungulates from the southeastern United States. Concatenated total evidence phylogenetics based on combined DNA sequence analyses from mitochondrial genes (12SrDNA, 16SrDNA and COI) and a nuclear gene (ITS2) corroborate the recognition of these species.
      
      

    • Low Genetic Diversity of the Only Clade of the Tick Rhipicephalus microplus in the Neotropics (Completo, 2023)

      Díaz-Sánchez, S. , Hernández-Triana, L.M. , Labruna, M.B. , Merino , O. , Mosqueda, J. , Nava, S. , Szabó, M. , Tarragona, E. , VENZAL, J.M. , de la Fuente, J. , Estrada-Peña, A.
      Pathogens, v.: 12 11 , p.:1344 - 1344, 2023
      Palabras clave: Rhipicephalus microplus COI; 16S rDNA ITS-2 Neotropics clade A climate traits evolution rates
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 20760817
      DOI: 10.3390/pathogens12111344
      This study addresses the variability of the mitochondrial cytochrome oxidase subunit I (COI) and 16S rDNA (16S), and nuclear internal transcriber spacer ITS2 (ITS2) genes in a set of field-collected samples of the cattle tick, Rhipicephalus microplus (Canestrini, 1888), and in geo-referenced sequences obtained from GenBank. Since the tick is currently considered to be a complex of cryptic taxa in several regions of the world, the main aims of the study are (i) to provide evidence of the clades of the tick present in the Neotropics, (ii) to explore if there is an effect of climate traits on the divergence rates of the target genes, and (iii) to check for a relationship between geographical and genetic distance among populations (the closest, the most similar, meaning for slow spread). We included published sequences of Rhipicephalus annulatus (Nearctic, Afrotropical, and Mediterranean) and R. microplus (Afrotropical, Indomalayan) to fully characterize the Neotropical populations (total: 74 16S, 44 COI, and 49 ITS2 sequences included in the analysis). Only the clade A of R. microplus spread in the Nearctic?Neotropics. Both the K and Lambda?s statistics, two measures of phylogenetic signal, support low divergence rates of the tested genes in populations of R. microplus in the Neotropics. These tests demonstrate that genetic diversity of the continental populations does not correlate either with the geographic distance among samples orwith environmental variables. The lowvariability of these genesmay be due to a combination of factors like (i) the recent introduction of the tick in the Neotropics, (ii) a large, effective, and fast exchange of populations, and (iii) a low effect of climate on the evolution rates of the target genes. These results have implications for the ecological studies and control of cattle tick infestations.
      
      

    • Parasitismo de Amblyomma aureolatum (Pallas, 1772) (Acari: Ixodidae) en humanos y determinación molecular de organismos rickettsiales y piroplasmas en el norte de Uruguay (Completo, 2023)

      Alvez. R. , FÉLIX, M.L. , Diego Queirolo , VENZAL, J.M.
      FAVE Sección Ciencias Veterinarias, v.: 22 p.:26 - 26, 2023
      Palabras clave: Amblyomma aureolatum Rickettsia bellii Candidatus Midichloria sp. Rangelia vitalii Uruguay
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
      Medio de divulgación: Internet
      ISSN: 1666938X
      E-ISSN: 23625589
      DOI: 10.14409/favecv.2023.22.e0026
      Amblyomma aureolatum (Pallas, 1772) se distribuye en Sudamérica y posee importancia médica y ve-terinaria. En Uruguay se encuentra ampliamente distribuida parasitando vertebrados silvestres y domésticos y se la relaciona a casos de rangeliosis canina. El objetivo de este trabajo fue determinar la presencia de orga-nismos rickettsiales y piroplasmas en A. aureolatum del norte de Uruguay. Para ello se recolectaron 117 ninfas y adultos de A. aureolatum sobre zorros, perros y vegetación, así como una sobre un humano. Se extrajo ADN de las garrapatas obtenidas de animales y vegetación para realizar diferentes PCR utilizando cebadores dirigidos a genes para la detección de ADN de organismos Rickettsiales (Anaplasmataceae, Rickettsiaceae y Midichloriaceae) así como protozoarios del Orden Piroplasmida. Como resultados se obtuvieron tres secuencias, una para el gen gltA con una identidad de entre 99,4 a 100% con Rickettsia bellii, otra para el gen 16S ARNr con un 100% de identidad con Candidatus Midichloria sp. y la restante con 100% de identidad con Rangelia vitalii para el gen 18S ARNr. Estos resultados corresponden a los primeros reportes de R. bellii para el país y de Ca. Midichloria sp. y R. vitalii en A. aureolatum. Además, se reporta el primer caso de A. aureolatum parasitando humanos en Uruguay.
      
      

    • Morphological and molecular analysis of Ornithodoros hasei and Ornithodoros sp. cf. O. mimon (Acari: Argasidae) from northeastern Argentina. (Completo, 2022)

      Di Benedetto, I.M.D. , Debarbora, V.N. , Benitez Ibalo, A.P. , Oscherov, E.B. , Autino, A.G. , Nava, S.
      Systematic and Applied Acarology, v.: 27 1 , p.:71 - 80, 2022
      Palabras clave: ectoparasites ticks larvae Chiroptera Corrientes Argentina
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Internet
      E-ISSN: 20566069
      DOI: https://doi.org/10.11158/saa.27.1.8
      In this study two species of soft ticks belonging to the genus Ornithodoros were recorded in three areas in the province of Corrientes, Argentina. Four larvae were identified as Ornithodoros hasei (Schulze, 1935) on Molossus molossus (Molossidae) in Paraje Tres Cerros (Department of San Martín), while 56 larvae were identified as Ornithodoros sp. cf. O. mimon Kohls, Clifford & Jones, 1969 on Eptesicus furinalis and Myotis levis (Vespertilionidae) in Paraje Galarza (Department of Santo Tomé) and Colonia Carlos Pellegrini (Department of San Martín). These last two locations are found within the Esteros del Iberá eco-region. The known distribution range of O. hasei and O. cf. O. mimon is expanded to include the province of Corrientes. Two new ectoparasite-host associations were recorded for Argentina between O. hasei on M. molossus and O. cf. O. mimon on M. levis. The morphological differences together with the results of the phylogenetic analysis show that O. hasei presents genetic similarity with specimens from Brazil, and in Argentina, with ticks from Santa Fe. Instead, Ornithodoros sp. cf. O. mimon recorded in Corrientes and Uruguay belong to a different taxon than the O. mimon recorded in Brazil and Santa Fe, Argentina. Based on this, O. mimon should be considered a complex of species in which the specimens from Corrientes are provisionally considered as Ornithodoros sp. cf. O. mimon until their taxonomic status can be determined.
      
      

    • Rickettsia spp., Ehrlichia sp. and Candidatus Midichloria sp. associated to ticks from a protected urban area in Buenos Aires City (Argentina). (Completo, 2022)

      Gabriel L. Cicuttin , María N. De Salvo , VENZAL, J.M. , Nava, S.
      Experimental and Applied Acarology, v.: 86 p.:271 - 282, 2022
      Palabras clave: Rickettsia Ehrlichia Midichloriaceae Ticks Buenos Aires City Argentina
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Medio de divulgación: Internet
      E-ISSN: 15729702
      DOI: https://doi.org/10.1007/s10493-022-00684-0
      The aim of this study was to determine the infection with Rickettsiales in ticks and birds from the main protected urban area of Buenos Aires City (Argentina). One Amblyomma aureolatum (0.2%) and one Ixodes auritulus (0.1%) were positive by PCR targeting Rickettsia 23S-5S rRNA intergenic spacer. Phylogenetic analysis shows to findings in A. aureolatum are closely to Rickettsia bellii and for I. auritulus are related to ?Candidatus Rickettsia mendelii?. One I. auritulus (0.1%) and three A. aureolatum (0.6%) were positive by PCR for a fragment of the 16S rRNA gene of the Anaplasmataceae family. The sequences obtained from A. aureolatum were phylogenetically related to Midichloriaceae endosymbionts. The sequence from I. auritulus s.l. had 100% identity with Ehrlichia sp. Magellanica from Chile and two genotypes of Ehrlichia sp. from Uruguay. The results of our study show that Rickettsia and Ehrlichia are present in ticks in the main protected urban area of Buenos Aires City.
      
      

    • Ticks infesting humans in Central America: A review of their relevance in public health (Completo, 2022)

      Bermúdez C., S. , Domínguez A., L. , Troyo, A. , Montenegro H., V.M. , VENZAL, J.M.
      Current Research in Parasitology and Vector-Borne Diseases, v.: 2 p.:1 - 6, 2022
      Palabras clave: Ticks Humans Environments Tick-borne diseases Allergies Paralysis Central America
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Medio de divulgación: Internet
      ISSN: 2667114X
      DOI: https://doi.org/10.1016/j.crpvbd.2021.100065.
      Ticks are blood-sucking arthropods that parasitize most groups of terrestrial or semiaquatic vertebrates. Humans are accidental hosts to the ticks; however, in humans the ticks can cause damages varying from simple irritation to severe allergies, toxicosis, paralysis, and the transmission of pathogens, some of which can be fatal. Central America represents a narrow isthmus between North and South America and is considered a biodiversity hotspot. The importance of tick borne diseases in this region is manifested by fatal outbreaks caused by Rickettsia rickettsii, severe and mild cases of other rickettsioses, ehrlichiosis, and tick-borne relapsing fevers, in addition to cases paralysis and strong allergic reactions. Even so, this information is scarce in most countries of this region, and there are no epidemiological data. In this article we present a review of the ticks that parasitize humans in Central America, covering data from the 19th Century to the present day. Of nearly 80 tick species reported in Central America, 28 species are reported on humans. This list includes species that thrive within homes, grazing areas and, to a lesser extent, in wild environments, both in lowland and high mountain forests. The most important genus in this region is Amblyomma, followed by Rhipicephalus and Ornithodoros, and to a lesser extent Haemaphysalis, Ixodes and Dermacentor. These data provide information on the tick species most commonly associated with humans in Central America, and highlight the potential for tick-borne diseases in wild, rural and urban regions.
      
      

    • Description of bovine babesiosis and anaplasmosis outbreaks in northern Uruguay between 2016 and 2018. (Completo, 2022)

      Parodi, P- , ARMUA-FERNANDEZ, M. T.; ARMUA, M.T. , Corbellini, L.G. , RIVERO, R , Miraballes, C. , Riet-Correa F. , VENZAL, J.M.
      Veterinary Parasitology. Regional Studies and Reports, v.: 29 p.:1 - 6, 2022
      Palabras clave: Hemiparasites Anaplasma marginale Babesia spp. Cattle Epidemiology Uruguay
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 24059390
      DOI: https://doi.org/10.1016/j.vprsr.2022.100700
      Bovine babesiosis and anaplasmosis cause important economic losses in livestock production. In Uruguay, the main aetiological agents of bovine babesiosis and anaplasmosis are Babesia bovis, B. bigemina and Anaplasma marginale. The aim of this work was to describe the outbreaks of bovine babesiosis and anaplasmosis in northern Uruguay between 2016 and 2018. Convenience sampling was carried out. We worked with blood and organ samples from bovines with clinical signs and autopsy findings compatible with babesiosis and anaplasmosis. A total of 140 presumptive outbreaks were studied. Epidemiological information such as place, date of occurrence, age, number of sick and dead animals, clinical signs, autopsy findings, the presence of ticks and health management that involved injectables were registered. The diagnoses were carried out by blood and organ smears stained with Giemsa and confirmed by multiplex PCR. There were 83 (59.2%) positive outbreaks, comprising 35 (42.2%) A. marginale, 19 (22.9%) B. bigemina, 18 (21.7%) B. bovis and 11 (13.2%) mixed infections (Babesia spp. + A. marginale). Cows were the most commonly affected category. The clinical signs and autopsy findings with a significant association (p ? 0.05) were anaemia, pale mucous membranes, fever, jaundice, ataxia and aggressiveness, splenomegaly, and orange discolouration of the liver. Babesiosis had a seasonal occurrence, mainly in autumn, while anaplasmosis cases were recorded throughout the year. The use of injectable agents was associated with A. marginale transmission. This work contributes updated information about epidemiological and clinical patterns of bovine babesiosis and anaplasmosis in northern Uruguay, which is important for implementing preventive measures and control.
      
      

    • Characterization of strains of Anaplasma marginale from clinical cases in bovine using major surface protein 1a in Uruguay. (Completo, 2022)

      Parodi, P , María Teresa ARMÚA FERNÁNDEZ , Schanzembach, M. , Mir, D. , Benítez-Galeano, M.J. , Rodríguez-Osorio, N. , Rivero, R. , VENZAL, J.M.
      Frontiers in Veterinary Science, v.: 9 p.:99022 - 99022, 2022
      Palabras clave: bovine Anaplasma marginale genotyping MSP1a Uruguay
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 22971769
      DOI: 10.3389/fvets.2022.990228
      The major surface protein 1a (MSP1a) gene has been used to characterize Anaplasma marginale genetic diversity. This pathogen causes significant productivity and economic losses to the cattle industry. The objective of the present study was to report the first characterization of A. marginale genetic diversity in Uruguay based on MSP1a genotypes and their putative relationship with Rhipicephalus microplus. This cross-sectional study was conducted between 2016 and 2020. The study included whole blood samples from clinical cases of bovine anaplasmosis obtained from 30 outbreaks located in six Uruguay territorial departments. Diagnosis was performed using Giemsa stained smears and confirmed by nested Polymerase Chance Reaction (nPCR) targeting the A. marginale major surface protein 5 gene. The genetic diversity of A. marginale strains was characterized by analyzing the microsatellite and tandem repeats of MSP1a. Based on the microsatellite structure, four genotypes were identified. Genotype E was the most prevalent. Analysis of MSP1a tandem repeats showed 28 different strains from the combination of 31 repeats with ?-10-15 and ? - ?- ?- ?- ? being the most common. Repeats ?, ?, ?, and g were associated with the absence of R. microplus with statistical significance (p < 0.05). Molecular observations showed that 46.7% of the strains identified in our samples lacked the ability to bind to tick cells; therefore, they were probably transmitted by other vectors. Strain genetic diversity provides valuable information for understanding the epidemiological behavior of A. marginale and could contribute to the development of effective vaccines for the control of this disease.
      
      

    • One Health Approach to Identify Research Needs on Rhipicephalus microplus Ticks in the Americas. (Completo, 2022)

      Estrada-Peña, A. , Rodríguez Mallón, A. , Bermúdez, S. , de la Fuente, J. , Domingos, A. , Estrada García, M.P. , Labruna, M.B. , Merino, O. , Mosqueda, J. , Nava, S. , Lleonart Cruz, R. , Szabó, M. , Tarragona, E. , VENZAL, J.M.
      Pathogens, v.: 11 1180 , p.:1 - 13, 2022
      Palabras clave: Rhipicephalus microplus ecology climate pasture vacancy integrated control One Health research agenda
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 20760817
      DOI: 10.3390/pathogens11101180
      We aim to provide a harmonized view of the factors that affect the survival and promote the spread of R. microplus in the Neotropics, approaching its different facets of biology, ecology, distribution, and control. We review the interactions among environmental niche, landscape fragmentation, vegetal coverage (abiotic traits), and the biotic aspects of its ecology (abundance of domesticated or wild competent hosts), proposing emerging areas of research. We emphasize a holistic view integrating an economically and ecologically sustainable control of infestations and transmitted pathogens by R. microplus in the Neotropics. Examples of research link the trends of climate, the composition of the community of hosts, the landscape features, and a tailored management based on ecological grounds. Our view is that factors driving the spread of R. microplus are complex and deeply interrelated, something that has been seldom considered in control strategies. The effects of climate may affect the dynamics of wildlife or the landscape composition, promoting new patterns of seasonal activity of the tick, or its spread into currently free areas. In this paper we encourage a One Health approach highlighting the main aspects governing the components of the tick?s life cycle and its interactions with livestock and wild animals.
      
      

    • Editorial: Soft ticks as parasites and vectors. (Reseña, 2022)

      Mans, B.J. , Muñoz-Leal, S. , VENZAL, J.M.
      Frontiers in Veterinary Science, v.: 9 p.:1 - 3, 2022
      Palabras clave: ecology bloodmeal salivary glands distribution
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      Escrito por invitación
      E-ISSN: 22971769
      DOI: 10.3389/fvets.2022.1037492
      Soft ticks are interesting ectoparasites due to their ecological habits and unique blood-feeding biology. The wide-ranging nature of research into these parasites is reflected in the diverse set of papers captured in this special issue. Fundamental questions are addressed in the 9 articles included in this Research Topic that focuses on ecology, tick-host interaction, host associations, geographic distribution, and microbial endosymbionts.
      
      

    • The use of flow cytometry for diagnosis and immunophenotyping in chronic lymphocytic leukemia in a dog: Clinical case report. (Completo, 2022)

      Sánchez-Solé, R. , Pedreira, G. , VENZAL, J.M. , Fonseca-Alves, C.E. , Pessina Serdio, P.
      Open Veterinary Journal, v.: 12 6 , p.:868 - 876, 2022
      Palabras clave: Canine Cytotoxic T lymphocytes Chronic leukemia Immunophenotype Lymphocytosis
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
      Medio de divulgación: Internet
      ISSN: 22264485
      E-ISSN: 22186050
      DOI: 10.5455/OVJ.2022.v12.i6.13
      
      

    • Ixodes silvanus n. sp. (Acari: Ixodidae), a new member of the subgenus Trichotoixodes Reznik, 1961 from northwestern Argentina. (Completo, 2021)

      Saracho Bottero, M.N. , Beati, L. , VENZAL, J.M. , Guardia, L. , Thompson, C.S. , Mangold, A.J. , Guglielmone, A.A. , Nava, S.
      Ticks and Tick-borne Diseases, v.: 12 101572 , p.:1 - 11, 2021
      Palabras clave: Ixodes Trichotoixodes New species Argentina
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: https://doi.org/10.1016/j.ttbdis.2020.101572
      Females, nymphs, and larvae of Ixodes silvanus n. sp. collected from birds and from the vegetation in northwestern Argentina (Yungas Phytogeographic Province) are described herein. The new species belongs to the subgenus Trichotoixodes (Acari: Ixodidae). The female is diagnosed by a combination of the following characters: scutum with setae moderately long and more numerous in central field, fewer and moderately long setae on lateral fields, and inconspicuous setae in anterior field; basis capituli subtriangular dorsally; porose areas large and irregular in shape, lacking distinct margins; auriculae with straight edges diverging posterolaterally and ending with small blunt processes; hypostome narrow and pointed with dental formula 4/4 in the anterior third, then 3/3 and 2/2 near the base; coxae I with two spurs, sub-equal in size, internal slightly slimmer than external. The nymph is diagnosed by notum with numerous and long setae, ventral surface covered by numerous whitish setae, scutum with short scapulae and few and shallow punctations, setae on scutum few, short and irregularly distributed, basis capituli sub-triangular dorsally with posterior margin straight, cornua large and directed postero-laterally, auriculae large and projected laterally, lateral margin of basis capituli above auriculae with a lateral and triangular projection, hypostome pointed with dental formula 3/3 in the anterior third and then 2/2, and coxa I with two short, sub-equal, triangular spurs. The diagnostic characters of the larva are: basis capituli dorsally sub-triangular with lateral angles acute and posterior margin straight, auriculae as large triangular lateral projections, hypostome with apex bluntly pointed and dental formula 3/3 in the anterior third and then 2/ 2, coxa I with two short, sub-equal, triangular spurs, and pattern of dorsal and ventral body setae. This new species is phylogenetically related to Ixodes brunneus, Ixodes turdus and Ixodes frontalis, and the principal hosts for all its parasitic stages are birds.
      
      

    • Validation of a multiplex PCR assay to detect Babesia spp. and Anaplasma marginale in cattle in Uruguay in the absence of a gold standard test. (Completo, 2021)

      Parodi, P. , Corbellini, L.G. , Leotti, V.B. , Miraballes, C. , Rivero, R. , Riet-Correa, F. , VENZAL, J.M. , ARMUA-FERNANDEZ, M. T.
      Journal of Veterinary Diagnostic Investigation, v.: 33 1 , p.:73 - 79, 2021
      Palabras clave: Anaplasma marginale; Babesia spp.; cattle; multiplex PCR; sensitivity; specificity; tick-borne diseases
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 10406387
      E-ISSN: 19434936
      DOI: https://doi.org/10.1177/1040638720975742
      Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp 5 region of A. marginale. We used a Bayesian model with a non informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent.
      
      

    • Detección molecular de Hepatozoon felis en gatos con anemia en Uruguay. (Completo, 2021)

      Bazzano, V. , Freire, J. , Armúa-Fernández, M.T. , FÉLIX, M.L. , CARVALHO L , VENZAL, J.M.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 57 215 , p.:509 - 509, 2021
      Palabras clave: Hepatozoonosis Hepatozoon felis PCR gatos Uruguay anemia
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 03764362
      E-ISSN: 16884809
      DOI: https://dx.doi.org/10.29155/vet.57.215.4
      La hepatozoonosis felina es una enfermedad parasitaria causada por Hepatozoon spp., que generalmente cursa de manera subclínica y cuando se manifiesta lo hace en asociación con una inmunodeficiencia inducida por una enfermedad concomitante. El objetivo del presente estudio fue determinar la presencia de Hepatozoon spp. en gatos domésticos de Uruguay a través del diagnóstico mediante técnicas moleculares (PCR). Para ello se obtuvieron 230 muestras de sangre de gatos que previamente habían sido diagnosticados con anemia clínica, procedentes de nueve de los 19 departamentos del país. Para el diagnóstico molecular, dos PCRs independientes fueron realizadas amplificando dos fragmentos solapantes del gen 18S rARN de Hepatozoon spp. Nueve de las 230 muestras resultaron positivas a Hepatozoon spp., siendo la frecuencia de infección del 3,91%. La identidad de las secuencias obtenidas fue entre 99,10 y 100% con secuencias de Hepatozoon felis disponibles en GenBank. Así mismo, el análisis filogenético demostró que estas secuencias obtenidas de los gatos de Uruguay se agrupan en un clado con alto soporte Bootstrap con secuencias de H. felis procedentes de diferentes continentes. En base a estos resultados, se reporta por primera vez la presencia de H. felis infectando gatos domésticos en Uruguay.
      
      

    • Morphological descriptions of the nymph and adults of Ornithodoros clarki, the larva and nymph of Ornithodoros rondoniensis, with notes on their phylogenetic relationships. (Completo, 2021)

      Labruna, M.B. , Nava, S. , Rebollo-Hernández, A. , Guzmán-Cornejo, C. , Hernández, L.V. , Domínguez, L. , Muñoz Leal, S. , VENZAL, J.M.
      Systematic Parasitology, v.: 98 p.:231 - 246, 2021
      Palabras clave: Argasidae Taxonomic status Neotropical Region
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 01655752
      E-ISSN: 15735192
      DOI: https://doi.org/10.1007/s11230-021-09973-5
      Based on tick specimens collected recently in Mexico, Nicaragua, Panama and Brazil, we provide morphological descriptions of the nymph and adults of Ornithodoros clarki Jones \& Clifford, 1972 from the first three countries, and the larva and nymph of Ornithodoros rondoniensis (Labruna, Terassini, Camargo, Brandao, Ribeiro \& Estrada-Pena, 2008) from Brazil. Also, an analysis of mitochondrial 16S rDNA sequences was performed to analyze the phylogenetic relationships of these tick species. Adults and nymphs of O. clarki and O. rondoniensis are unique among the Argasidae family by presenting exceptionally large spiracular plates with small goblets, and an integument with smooth polygonal mammillae. However, these two species are morphologically distinct based on specific patterns of coxal folds, idiosomal mammillae and pilosity, and female genital flap. In contrast, the larvae of O. clarki and O. rondoniensis are morphologically identical, except for a general larger size of the former species; this slight difference is corroborated by Principal Component Analysis (PCA) by using 40 morphometric variables. Phylogenetic analyses including 16S rDNA partial sequences of different Ornithodoros taxa from Central and South America indicate that O. rondoniensis from Brazil diverges from O. clarki from Mexico, Nicaragua and Panama. However, phylogenetic distance separating both alleged species is similar or slightly lower than the distances depicted for conspecific populations of a few other Ornithodoros species. Nonetheless, our primary criterion to maintain O. rondoniensis as a valid species is because its adult and nymphal stages do present distinct
      
      

    • Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay. (Completo, 2021)

      FÉLIX, M.L. , Muñoz-Leal, S. , CARVALHO L , QUEIROLO, D. , Remesar Alonso, S. , Nava, S. , ARMUA-FERNANDEZ, M. T. , VENZAL, J.M.
      Current Research in Parasitology and Vector-Borne Diseases, v.: 1 p.:10002 - 10002, 2021
      Palabras clave: Anaplasmataceae; Ehrlichia; Novel genotypes; Phylogeny; Ixodes auritulus; Birds
      Medio de divulgación: Internet
      ISSN: 2667114X
      DOI: https://doi.org/10.1016/j.crpvbd.2021.100022
      Ehrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixodes auritulus collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of Ehrlichia DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 I. auritulus ticks were collected in four of the five locations sampled. Fragments of three loci (16S rRNA, dsb and groEL) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of Ehrlichia spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as Ehrlichia sp. Serrana and Ehrlichia sp. Laguna Negra. Both genotypes were closely related to Ehrlichia sp. Magellanica, a species detected in Ixodes uriae and Magellanic penguins. Considering that all stages of I. auritulus and I. uriae are parasites of birds, their phylogenetic relationships, and common eco-epidemiological profiles, it is reasonable to state that these genotypes of Ehrlichia spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of Ehrlichia spp. in Uruguay. Future studies on birds reported as hosts for I. auritulus are needed to further understand the epidemiological cycles of both Ehrlichia genotypes in the country. Finally, I. auritulus does not feed on humans, so the two Ehrlichia species reported herein might have no implications in human health.
      
      

    • A new species of soft tick from dry tropical forests of Brazilian Caatinga. (Completo, 2021)

      Muñoz-Leal, S. , VENZAL, J.M. , Jorge, F.R. , Teixeira, B. , Labruna, M.B.
      Ticks and Tick-borne Diseases, v.: 12 p.:10174 - 10174, 2021
      Palabras clave: Argasidae Ornithodoros soft tick taxonomy Brazil
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 18779603
      DOI: https://doi.org/10.1016/j.ttbdis.2021.101748
      Ornithodoros tabajara n. sp. is described from laboratory-reared larvae and adult specimens collected in the Brazilian Caatinga. This new species shares the ecological niche with Ornithodoros rietcorreai and is likely associated with colonial rodents of genus Kerodon. However, O. tabajara n. sp. is morphologically easy to distinguish from O. rietcorreai and other Neotropical Ornithodoros by a unique combination of characters: larva with 17 pairs of dorsal setae (seven anterolateral, three central and seven posterolateral), sub-oval dorsal plate, hypostome blunt apically with dentition formula 2/2 along its extension, only one pair of posthypostomal setae, six pairs of sternal setae, posteromedian setae absent, and leave-shaped anal valves; alive adults with whiteish islands of mammillae symmetrically distributed on dorsum (not visible in ethanol-preserved specimens), and median disk merging with posteromedian file. A phylogenetic analysis performed with mitochondrial 16S rDNA sequences points O. tabajara n. sp. as O. rietcorreai's sister taxon, which rises the hypothesis of sympatric speciation.
      
      

    • Ixodes tropicalis (Acari: Ixodidae) infesting a human and molecular detection of Rickettsia bellii, Colombia (Completo, 2021)

      Quintero, J.C. , FÉLIX, M.L. , VENZAL, J.M. , Nava, S.
      Biomédica, v.: 41 p.:347 - 352, 2021
      Palabras clave: Rickettsia bacteria disease vectors Ixodes
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología / Parasitología
      Medio de divulgación: Internet
      ISSN: 01204157
      E-ISSN: 25907379
      DOI: https://doi.org/10.7705/biomedica.5464
      Introduction: Ixodes tropicalis is a little-known tick species reported parasitizing wild rodents only in Colombia and Perú. Objective: To report a case of I. tropicalis infesting a human in the south of the metropolitan area of the Valle de Aburrá, Antioquia, Colombia, and to report the molecular detection of Rickettsia bellii in this species. Materials and methods: The tick was identified using a morphological key and sequencing of tick mitochondrial 16S rRNA. Additionally, bacterial and protozoa pathogens were evaluated using PCR for the detection of Rickettsia spp., family Anaplasmataceae, Borrelia spp., and piroplasmid. Results: We identified the tick as an I. tropicalis female according to Kohls, 1956, description and to partial 16S rRNA sequences showing a minimum of 5% divergencies compared to Ixodes sequences. We also detected the gltA gene of R. bellii in the tick with 99.87% of identity. Conclusion: This is the first report in Colombia of a species of the Ixodes genus parasitizing a human and the first report of the detection of R. bellii in this tick species.
      
      

    • Molecular screening for tick-borne bacteria and hematozoa in Ixodes cf. boliviensis and Ixodes tapirus (Ixodida: Ixodidae) from western highlands of Panama (Completo, 2021)

      Bermúdez C., S.E. , FÉLIX, M.L. , Domínguez A., L. , Kadoch, N. , Muñoz-Leal, S. , VENZAL, J.M.
      Current Research in Parasitology and Vector-Borne Diseases, v.: 1 p.:34 - 34, 2021
      Palabras clave: Ixodes cf. boliviensis Ixodes tapirus Rickettsia spp. endosymbionts Anaplasma phagocytophilum Borrelia burgdorferi (s.l.) complex Babesia odocoilei Hepatozoon sp. Panama
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Medio de divulgación: Internet
      ISSN: 2667114X
      DOI: https://doi.org/10.1016/j.crpvbd.2021.100034
      The first molecular screening for Rickettsia, Anaplasma, Ehrlichia, Borrelia, Babesia and Hepatozoon was carried out in questing Ixodes cf. boliviensis and Ixodes tapirus from Talamanca Mountains, Panama, using specific primers, sequencing and phylogeny. Phylogenetic analyses for the microorganisms in Ixodes cf. boliviensis confirmed the presence of Rickettsia sp. strain IbR/CRC endosymbiont (26/27 ticks), three genotypes of the Borrelia burgdorferi (sensu lato) complex (4/27 ticks), Babesia odocoilei (1/27 ticks), and Hepatozoon sp. (2/27 ticks), tentatively designated Hepatozoon sp. strain Chiriquensis. Phylogenetic analyses for the microorganisms in I. tapirus revealed an undescribed Rickettsia sp., tentatively designated Rickettsia sp. strain Itapirus LQ (6/6 ticks), and Anaplasma phagocytophilum (2/6 ticks). To the best of our knowledge, this is the first report of B. burgdorferi (s.l.) complex, A. phagocytophilum, B. odocoilei, and Hepatozoon sp. in Ixodes ticks from Central America, and also the first detection of Rickettsia spp. in Ixodes species in Panama. In light of the importance of these findings, further studies are needed focusing on the role of I. tapirus and I. cf. boliviensis as vectors, and the vertebrates acting as reservoirs.
      
      

    • Rangelia vitalii in free-living crab-eating foxes (Cerdocyon thous) in Uruguay. (Completo, 2021)

      De Lorenzo, C. , Boabaid, F.M. , Oliveira, L.G.S. , Bianchi, M.V. , FÉLIX, M.L. , ARMUA-FERNANDEZ, M. T. , Soares, J.F. , VENZAL, J.M. , Sonne, L.
      Ticks and Tick-borne Diseases, v.: 12 5 , p.:1 - 4, 2021
      Palabras clave: Wild canids Hemoparasites Rangelia vitalii Tick-borne disease
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: https://doi.org/10.1016/j.ttbdis.2021.101765
      Rangelia vitalii is a protozoan parasite that causes a hemorrhagic and hemolytic disease in dogs known as rangeliosis. Current reports of the disease are concentrated in the southern and southeastern regions of Brazil, as well as in Uruguay, Argentina, and Paraguay, and mainly concern domestic dogs. South American wild canids, such as the crab-eating fox (Cerdocyon thous), the pampas fox (Lycalopex gymnocercus), and the maned wolf (Chrysocyon brachyurus) may also be affected, although existing reports are restricted to Brazil. The present study aimed to detect R. vitalii parasitism in the Uruguayan wild fox population. DNA extracted from the blood and/or spleen samples of road-killed C. thous and L. gymnocercus found in northern Uruguay were subjected to polymerase chain reaction (PCR) to amplify a 551-bp fragment of the Rangelia 18S rRNA gene. A total of 62 wild canids, including 38 C. thous and 24L. gymnocercus, were analyzed. Five crab-eating fox samples (13.2%) were positive for R. vitalii, with 99.5?100% identity between the sequences. All samples from pampas fox tested negative for R. vitalii. When compared with the R. vitalii sequences available in GenBank, a similarity of 98.9?100% was revealed. Molecular analysis results suggest that R. vitalii is circulating in the crab-eating fox population in Uruguay; however, its veterinary relevance for these foxes remains unknown.
      
      

    • An Hepatozoon americanum?like protozoan in crab?eating (Cerdocyon thous) and grey pampean (Lycalopex gymnocercus) foxes from Uruguay. (Completo, 2021)

      CARVALHO L , FÉLIX, M.L. , Bazzano, V. , da Costa, A. , ARMUA-FERNANDEZ, M. T.; ARMUA, M.T. , Muñoz?Leal, S. , VENZAL, J.M.
      Parasitology Research, v.: 120 p.:3587 - 3593, 2021
      Palabras clave: Hepatozoon Molecular detection Cerdocyon thous Lycalopex gymnocercus Uruguay
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 14321955
      DOI: https://doi.org/10.1007/s00436-021-07305-6
      In South America, apicomplexan parasites of the genus Hepatozoon have been sporadically detected in mammals. Previous studies in wild canids from Brazil and Argentina demonstrated infections by species genetically related to Hepatozoon americanum. The aim of the present work was to detect the presence of Hepatozoon in road-killed foxes encountered in Uruguayan highways. Blood samples from 45 crab-eating (Cerdocyon thous) and 32 grey pampean (Lycalopex gymnocercus) foxes were analyzed by PCR for Hepatozoon 18S rRNA gene. Eight foxes (10.4%) were found to be infected with an H. americanum-like protozoan, an Hepatozoon closely related to H. americanum. Bayesian and maximum-likelihood phylogenetic analyses revealed that the sequences obtained in this study cluster with H. Americanum from the United States, and with an H. americanum-like species from dog and foxes from Brazil and Argentina. In the Unites States, H. americanum causes severe disease in dogs. In addition to this, an increasing habitat overlap between dogs and foxes makes the presence of H. americanum-like protozoan in foxes acquires veterinary relevance. This work represents the first report of L. gymnocercus infected with an H. americanum-like protozoan, and of wild canids infected with Hepatozoon in Uruguay.
      
      

    • Niche divergence among closely related taxa provides insight on evolutionary patterns of ticks. (Completo, 2021)

      Cuervo, P.F. , Flores, F.S. , VENZAL, J.M. , Nava, S.
      Journal of Biogeography, v.: 48 p.:2865 - 2876, 2021
      Palabras clave: allopatry ecological niche model environmental space niche conservatism niche divergence tick speciation
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Ecología / Biogeografía
      Medio de divulgación: Internet
      E-ISSN: 13652699
      DOI: 10.1111/jbi.14245
      Aim: Understanding the degree to which closely related taxa diverge in their niche traits could provide insight on their evolutionary patterns, as well as shed some light on the mechanisms underpinning broad-scale biogeographic patterns. The evolution of ticks was thought to be driven by hosts. However, recent evidence suggests that tick evolution is more likely to be driven by habitat conditions. The Amblyomma maculatum group of ticks provides a good example to test the former, as its incipient speciation raises the possibility of a very rapid adaptation to slightly different environments. Location: The Americas. Taxa: Ticks from the Amblyomma maculatum Koch, 1844 ( Acari: Ixodidae) group (A. tigrinum, A. triste s.s., A. maculatum s.s. and two intermediate morphs). Methods: We addressed the question of whether the differentiation of taxa within this group results from ecological factors, either maintaining a similar ecological niche (conservatism) or by occupying distinct niches (divergence). We analysed the distribution of each tick morphotype with ecological niche models. Next, we explored the question of whether these closely related taxa inhabit environments that are more different or more similar than expected by comparing niche overlap in environmental space. Results: We found evidence for niche differentiation, showing that the members of the Amblyomma maculatum group exist in and respond to aspects of different environments, leading to geographical variation. Main conclusions: The analysis of the ecological niches of the Amblyomma maculatum group of ticks indicates niche conservatism for the pairs A. tigrinum?A. maculatum s.s. and A. triste s.s.?A. maculatum s.s, traditionally associated to allopatric speciation; while incipient niche divergence is suggested for the remaining comparisons. These findings add additional evidence to the study of the evolution of ticks, giving support to the hypothesis of habitat conditions driving the evolution of taxa with no strict host specificity.
      
      

    • Molecular Characterization of Spirometra decipiens Complex (Eucestoda: Diphyllobothriidea) from Uruguay. (Completo, 2021)

      ARMUA-FERNANDEZ, M. T.; ARMUA, M.T. , Burutarán, M. , Bazzano, V. , FÉLIX, M.L. , CASTRO, O. , VENZAL, J.M.
      Taxonomy, v.: 1 p.:270 - 277, 2021
      Palabras clave: Spirometra decipiens cox1 gene phylogeny South America Uruguay
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 26736500
      DOI: https://doi.org/10.3390/taxonomy1030021
      https://www.mdpi.com/journal/taxonomy
      This study used a partial sequence of the mitochondrial cox1 gene for the reconstruction of the interrelationship of the adult and larval stages of Spirometra obtained from Cerdocyon thous, Leopardus munoai, Canis familiaris, Didelphis albiventris and Philodryas patagoniensis in Uruguay. The phylogenetic analysis showed that they were grouped with Spirometra decipiens from the Americas with a high bootstrap support. According to recent studies, American Spirometra species split into two S. decipiens complexes. Our findings strongly suggest that S. decipiens complex 1 is widely distributed in South America, and that wild and domestic canids are definitive hosts. Most of the samples (n = 10) grouped adults and plerocercoids that were retrieved from mammals and reptiles and seem to be the same taxon found in a Lycalopex gymnocercus from Argentina. A second clade was formed with Spirometra sp. found in a L. munoai as well as other wild felids such as a Puma concolor and a Leopardus pardalis (both from Argentina). On the other hand, S. decipiens complex 2 is present in South America and North America. South American clade parasitizes wild (and possibly domestic) felids and reptiles as definitive and intermediate hosts, respectively, whereas the North American clade found in snakes and captive meerkats (acting either as second intermediate or paratenic hosts) has unconfirmed definitive hosts.
      
      

    • Characterization of Candidatus Ehrlichia Pampeana in Haemaphysalis juxtakochi Ticks and Gray Brocket Deer (Mazama gouazoubira) from Uruguay. (Completo, 2021)

      FÉLIX, M.L. , Muñoz-Leal, S. , CARVALHO L , QUEIROLO, D. , Remesar, S. , ARMUA-FERNANDEZ, M. T.; ARMUA, M.T. , VENZAL, J.M.
      Microorganisms, v.: 9 p.:2165 - 2165, 2021
      Palabras clave: Rickettsiales Anaplasmataceae Ehrlichia molecular characterization ticks Haemaphysalis juxtakochi gray brocket deer Uruguay
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Medio de divulgación: Internet
      E-ISSN: 20762607
      DOI: https://doi.org/10.3390/microorganisms9102165
      Human ehrlichiosis are scantily documented in Uruguay. The aim of this study was to investigate the presence of Ehrlichia spp. in Haemaphysalis juxtakochi and in a gray brocket deer (Mazama gouazoubira) from Uruguay. The presence of Ehrlichia DNA was investigated in free-living H. juxtakochi in five localities of southeast and northeast Uruguay, as well as blood, spleen, and ticks retrieved from a M. gouazoubira. Ehrlichia spp. DNA was detected in six out of 99 tick pools from vegetation, in the spleen of M. gouazoubira, and in one out of five pools of ticks feeding on this cervid. Bayesian inference analyses for three loci (16S rRNA, dsb, and groEL) revealed the presence of a new rickettsial organism, named herein as ?Candidatus Ehrlichia pampeana?. This new detected Ehrlichia is phylogenetically related to those found in ticks from Asia, as well as Ehrlichia ewingii from USA and Cameroon. Although the potential pathogenicity of ?Ca. E. pampeana? for humans is currently unknown, some eco-epidemiological factors may be relevant to its possible pathogenic role, namely: (i) the phylogenetic closeness with the zoonotic agent E. ewingii, (ii) the evidence of H. juxtakochi parasitizing humans, and (iii) the importance of cervids as reservoirs for zoonotic Ehrlichia spp. The molecular detection of ?Ca. E. pampeana? represents the third Ehrlichia genotype described in Uruguay.
      
      

    • Description of human infestations by ticks in Panama and Costa Rica. (Completo, 2021)

      Bermúdez, S. , Greco-Mastelari, V. , Zaldívar, Y. , Hernández, M. , Domínguez L. , Montenegro, V.M. , VENZAL, J.M.
      Microbes Infection and Chemotherapy, v.: 1 e1241, p.:1 - 7, 2021
      Palabras clave: tick bites humans lymphadenopathy erythema migrans-like environments Panama Costa Rica
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Medio de divulgación: Internet
      E-ISSN: 27894274
      DOI: https://doi.org/10.54034/mic.e1241
      Tick bites in humans are associated with the transmission of pathogens, anaphylactic shock, paralysis, and secondary infections. In this work we described six cases of tick bites in patients from Panama and Costa Rica. These bites were provoked for adults of Ornithodoros puertoricensis, Amblyomma cf. oblongoguttatum, Amblyomma ovale, and Ixodes cf. boliviensis, and a nymph of Amblyomma mixtum. The relationships of these species of ticks with the environment are explained. Among the reactions observed in the patients there are blisters, maculo-papular rash, granuloma, lymphadenopathy, and erythema migrans-like rash, but none referred symptoms compatible with an infectious disease. In the collected ticks a PCR battery was developed to rule out Borrelia, Rickettsia, Anaplasmataceae, and Coxiella diseases to the ticks from the cases 1, 2, 3 and 4, with negative results. Although no pathogen infections were evidenced, these finding indicate that the tick bites constitute a public health problem what goes unnoticed in many countries.
      
      

    • In memoriam: a eulogy for Daniel González-Acuña, 1963-2020. (Completo, 2021)

      Muñoz-Leal, S. , Silva-De-La-Fuente, M.C. , Barros-Battesti, D.M. , Guglielmone, A.A. , VENZAL, J.M. , Nava, S. , Sepúlveda, M.S. , Tkach, V. , Kinsella, M. , Castillo, D.A.F. , Llanos-Soto, S. , Grandón-Ojeda, A. , Celis, J.E. , Espejo, W. , Jara-Carrasco, S. , Azat, C. , Martins, T.F. , Labruna, M.B. , Briceño, C. , Figueroa, R.A. , Corales-Stappung, S. , Moreno, L.
      Revista Brasileira De Parasitologia Veterinaria, v.: 30(1) e000821 , p.:1 - 3, 2021
      Medio de divulgación: Internet
      ISSN: 0103846X
      E-ISSN: 19842961
      DOI: http://dx.doi.org/10.1590/S1984-29612021005
      
      
      

    • The Ixodes ricinus complex (Acari: Ixodidae) in the Southern Cone of America: Ixodes pararicinus, Ixodes aragaoi, and Ixodes sp. cf. I. affinis. (Completo, 2020)

      Saracho-Bottero, M.N. , VENZAL, J.M. , Tarragona, E.L. , Thompson, C.S. , MANGOLD, A.J. , Beati, L. , Guglielmone, A.A. , NAVA, S.
      Parasitology Research, v.: 119 p.:43 - 54, 2020
      Palabras clave: Ixodidae Ixodes ricinus complex Systematic Phylogeny South America
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Ciencias de la Salud / Parasitología
      Medio de divulgación: Internet
      ISSN: 09320113
      E-ISSN: 14321955
      DOI: https://doi.org/10.1007/s00436-019-06470-z
      The goal of this study was to clarify the taxonomic status of the Ixodes ricinus complex in the Southern Cone of America, by using morphological characters and molecular markers (mitochondrial 16SrDNA and cox1 genes). The morphological analysis indicates that three different taxa of the I. ricinus complex occur in this region: Ixodes pararicinus, Ixodes aragaoi, and Ixodes sp. cf. I. affinis. The most prominent diagnostic character among them is the size of scutal punctations in both male and female ticks. In themales of Ixodes sp. cf. I. affinis, the punctations on the central field and along themedianmarginal groove of the scutum are clearly larger than in the males of I. aragaoi and I. pararicinus, while the punctations of I. aragaoi are larger but less numerous than in I. pararicinus. The punctations in Ixodes sp. cf. I. affinis females are larger and deeper than in females of I. aragaoi and I. pararicinus, and those of I. aragaoi are slightly larger than in I. pararicinus. The length of the lateral posterior denticles of the male hypostome is comparatively longer in I. aragaoi than in the other two species, and longer in Ixodes sp. cf. I. affinis than in I. pararicinus. In the 16S analysis, I. pararicinus and I. aragaoi are monophyletic (99% and 98% bootstrap support, respectively), while Ixodes cf. I. affinis does not represent a single lineage. In the cox1 analysis, both I. pararicinus and I. aragaoi are welldefined taxa, but the bootstrap support for Ixodes sp. cf. I. affinis is low (67%). In general, there are considerable 16SrRNA differences among lineages of Ixodes sp. cf. I. affinis from different geographical areas. These results may be indicative of the existence of different species. The populations morphologically compatible with I. affinis from Argentina, Colombia, Panama, Belize, and USA should be provisionally named as Ixodes sp. cf. I. affinis until an integrative taxonomic work with further evidence redefines whether or not this taxon actually represents a species complex.
      
      

    • Towards an Effective, Rational and Sustainable Approach for the Control of Cattle Ticks in the Neotropics. (Completo, 2020)

      Estrada-Peña, A. , Szabó, M. , Labruna, M. , Mosqueda, J. , Merino, O. , Tarragona, E. , VENZAL, J.M. , de la Fuente, J.
      Vaccines, v.: 8 p.:9 - 9, 2020
      Palabras clave: Tick vaccine ecology neotropics cattle wildlife pathogen
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
      Medio de divulgación: Internet
      E-ISSN: 2076393X
      DOI: 10.3390/vaccines8010009
      Ticks and transmitted pathogens constitute a major burden for cattle industry in the Neotropics. To address this limitation, the Spanish Ibero-American Program of Science and Technology in Development office (CYTED) supported from 2018 a network of scientists named ?LaGar? (CYTED code 118RT0542) aimed at optimizing the control strategies of cattle ticks in the neotropical region. As part of network activities, a meeting and course were organized on 4?8 November 2019 in Querétaro, Mexico to address the objective of developing the infrastructure necessary for an effective, sustainable (i.e., combination of efficacious acaricides with anti-tick vaccines) and rational (i.e., considering tick ecology, seasonal dynamics and cattle-wildlife interactions) control of cattle tick infestations and transmitted pathogens. The course was focused on scientists, students, cattle holders and producers and pharmaceutical/industry representatives. In this way the course addressed the different views presented by participants with the conclusion of producing a research-driven combination of different interventions for the control of tick tick-borne diseases.
      
      

    • Borrelia burgdorferi sensu lato infecting Ixodes auritulus ticks in Uruguay (Completo, 2020)

      Carvalho, L.A. , Maya, L. , Armua?Fernandez, M.T. , Félix, M.L. , Bazzano, V. , Barbieri, A.M. , González, E.M. , Lado, P. , Colina, R. , Díaz, P. , Labruna, M.B. , Nava, S. , VENZAL, J.M.
      Experimental and Applied Acarology, v.: 80 p.:109 - 125, 2020
      Palabras clave: Borrelia burgdorferi sensu lato Ixodes auritulus Vegetation Birds Uruguay
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Ciencias de la Salud / Zoonosis
      Medio de divulgación: Internet
      ISSN: 01688162
      E-ISSN: 15729702
      DOI: doi: https://doi.org/10.1007/s10493-019-00435-8
      In the southern cone of South America different haplotypes of Borrelia burgdorferi sensu lato (Bbsl) have been detected in Ixodes spp. from Argentina, southern Brazil, Chile, and Uruguay. So far, Lyme borreliosis has not been diagnosed in Uruguay and the medical relevance of the genus Ixodes in South America is uncertain. However, the growing number of new genospecies of Bbsl in the southern cone region and the scarce information about its pathogenicity, reservoirs and vectors, highlights the importance of further studies about spirochetes present in Uruguay and the region. The aim of this study was to determine the presence of Bbsl in Ixodes auritulus ticks collected from birds and vegetation in two localities of southeastern Uruguay. In total 306 I. auritulus were collected from 392 passerine birds sampled and 1110 ticks were collected by flagging in vegetation. Nymphs and females were analyzed for Borrelia spp. by PCR targeting the flagellin (fla) gene and the rrfA-rrlB intergenic spacer region (IGS). The phylogenetic analysis of Borrelia spp. Positive samples from passerine birds and vegetation revealed the presence of four fla haplotypes that form a clade within the Bbsl complex. They were closely related to isolates of Borrelia sp. detected in I. auritulus from Argentina and Canada.
      
      

    • Synonymy of Ixodes aragaoi with Ixodes fuscipes, and reinstatement of Ixodes spinosus (Acari: Ixodidae) (Completo, 2020)

      Labruna, M.B. , Onofrio, V.C. , Barros-Battesti, D.M. , Gianizella, S.L. , VENZAL, J.M. , Guglielmone, A.A.
      Ticks and Tick-borne Diseases, v.: 11 p.:10134 - 10134, 2020
      Palabras clave: Ixodids Ticks Taxonomy South America
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: https://doi.org/10.1016/j.ttbdis.2019.101349
      Ixodes fuscipes Koch, 1844, described from Brazil, has been considered a valid species with records from Brazil and Peru. Ixodes spinosus Neumann, 1899, also described from Brazil, has been considered a synonym of I. fuscipes. In 2014, Ixodes aragaoi Fonseca, 1935 was redescribed as a valid species for Brazil and Uruguay. Until the present study, one major di.erence between the females of I. fuscipes and I. aragaoi was the horn-like auriculae in the former versus the ridge-like auriculae in the later, but this morphological di.erence was not supported after examination of the holotype of I. fuscipes and a syntype female of I. spinosus. Surprisingly, we found the type of I. fuscipes to have ridge-like auriculae, in contrast to the horn-like auriculae of I. spinosus. Comparisons of the I. fuscipes holotype with the syntypes of I. aragaoi revealed that they correspond to the same species. Therefore, we redescribe I. fuscipes, relegate I. aragaoi to a junior synonym of I. fuscipes, and reinstate I. spinosus as a valid species. After examining all lots of I. fuscipes in four tick collections, no specimen was recognized as I. fuscipes when compared with the type specimen of this taxon. On the other hand, specimens previously identi.ed as I. aragaoi are now con.rmed as I. fuscipes, with bona .de records for Brazil and Uruguay. Some of the specimens previously reported as I. fuscipes are now con.rmed as I. spinosus, with records in two Brazilian biomes, Amazon and Atlantic rainforest. We present lists of the ticks examined in this study, with their current taxonomic status.
      
      

    • Phylogenetic analysis of Hepatozoon spp. (Apicomplexa: Hepatozoidae) infecting Philodryas patagoniensis (Serpentes: Dipsadidae) in Uruguay (Completo, 2020)

      Bazzano, V. , Félix, M.L. , Parodi, P. , Carvalho, L.A. , Nava, S. , Armúa-Fernández, M.T. , VENZAL, J.M.
      Parasitology Research, v.: 119 p.:1093 - 1100, 2020
      Palabras clave: Hepatozoon Philodryas patagoniensis Phylogenetic analysis Uruguay
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 09320113
      E-ISSN: 14321955
      DOI: https://doi.org/10.1007/s00436-020-06605-7
      The purpose of this study was to perform a phylogenetic analysis of Hepatozoon spp. infecting Philodryas patagoniensis in Uruguay. Twenty-five road-killed specimens of P. patagoniensis from ten departments were obtained. Samples of blood and/or heart tissue were taken. Polymerase chain reaction (PCR) assay was carried out amplifying a specific target region of the 18S rRNA gene of Hepatozoon spp. Eighteen out of twenty-five samples were positive to Hepatozoon spp., which gave an overall prevalence of 72%. Phylogenetic analyses with the obtained sequences were carried out to determine the relationship with closely related species found in the region. The results revealed that samples were split into two clades with a high bootstrap support. Clade I was formed with Hepatozoon spp. sequences obtained in this study from P. patagoniensis, Hepatozoon cuestensis from Crotalus durissus terrificus and Hepatozoon musa from Philodryas nattereri, and Hepatozoon spp. retrieved from Cerdocyon thous, Hemidactylus mabouia, and Phyllopezus pollicaris from Brazil, respectively. Clade II was grouped with Hepatozoon cevapii and Hepatozoon massardii, both species described for C. d. terrificus from Brazil. This is the first report of Hepatozoon spp. in snakes from Uruguay.
      
      

    • Description of a new soft tick species (Acari: Argasidae: Ornithodoros) parasite of Octodon degus (Rodentia: Octodontidae) in northern Chile (Completo, 2020)

      Muñoz-Lealz-Leal, S. , VENZAL, J.M. , NAVA, S. , Marcili, A. , González-Acuña, D. , Martins, T.F. , Labruna, M:B.
      Ticks and Tick-borne Diseases, v.: 11 p.:10138 - 10138, 2020
      Palabras clave: Ornithodoros Octodontidae Octodon degus Chile
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: https:// doi.org/10.1016/j.ttbdis.2020.101385
      A new argasid (Argasidae) tick is herein described based on morphology and molecular data obtained from larvae parasitizing Octodon degus and from ticks collected inside burrows in northern Chile. Unfed laboratoryreared larvae were mounted in slides for morphometrical and morphological analyses. Larvae of Ornithodoros octodontus n. sp. share morphological traits with Ornithodoros quilinensis and Ornithodoros xerophylus, two species associated with rodents in the Argentinean Chaco. However, a longer hypostome with two rows of 21 and 22 denticles each one, and conspicuous leaf-shaped anal plates separate O. octodontus. While nymphal stages of O. octodontus lack cheeks and possess a micromammillated dorsal integument, adults have cheeks and exhibit markedly irregular mammillae along their dorsal surface. Phylogenetic analyses of neotropical Argasidae based on mitochondrial 16S rDNA sequences point that O. octodontus forms a monophyletic group with O. xerophylus and an unidentified Ornithodoros sp. from Bolivia, all of them associated with burrow dweller rodents. Ornithodoros aragaoi and Ornithodoros davisi, two rare species collected once only in the Peruvian Andean Plateau during 1955 are morphologically closely related with adults and nymphs of O. octodontus. Biological observations of O. octodontus revealed autogenic females. For the moment, subgeneric classification of this new species depends on further biological studies. The fauna of ticks occurring in Chile is now represented by 22 species, 11 belonging to the Argasidae family.
      
      

    • Redescription of the larva, and description of the nymphal and adult stages of Ornithodoros peruvianus Kohls, Clifford & Jones, 1969 (Acari: Argasidae) (Completo, 2020)

      Muñoz-Leal, S. , González-Acuña, D. , Labruna, M.B. , VENZAL, J.M.
      Systematic Parasitology, v.: 97 p.:201 - 215, 2020
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 01655752
      E-ISSN: 15735192
      DOI: https://doi.org/10.1007/s11230-020-09908-6
      The soft tick Ornithodoros peruvianus Kohls, Clifford & Jones, 1969 was described as a parasite of bats in Peru upon the examination of engorged larvae only. Recently, larvae of this tick species were reported on bats from northern Chile. However, the adult and nymphal stages of O. peruvianus have remained undescribed. This study aimed to redescribe the larva of O. peruvianus based on unfed specimens, and to describe nymphs, the male and the female of this species. Ticks were collected on the walls inside three caves in northern Chile. Two females laid eggs in the laboratory. Part of the unfed larvae was separated for morphological and morphometrical analyses, and the remaining specimens were fed upon laboratory mice in order to obtain subsequent nymphal and adult stages. The first nymphal stage (N1) moulted either to male or to a second nymphal stage (N2) without feeding. Obtained N2 moulted either to male or female after one meal. PCR amplification of tick mitochondrial 16S rRNA of specimens from the three caves revealed almost identical sequences. The unfed larva of O. peruvianus has an elongated idiosoma, and fringed setae cover the ventral surfaces of coxae, palps and tarsi. Nymph 1 has a thin integument covered by incipient mammillae and barely noticeable dorsal disks; it lacks cheeks and possesses few short setae on the basis capitulum. Nymph 2 has a pair of small cheeks and resembles adult stages in its tegumentary traits and capitulum. Adult stages exhibit developed cheeks (larger in females) without the capacity to completely cover the capitulum. Very small and low mammillae cover the surface of the dorsal idiosoma in adults. As this feature also occurs in other bat-associated soft ticks, regardless of their phylogenetic relatedness, small mammillae in bat soft ticks are suggestive of convergent evolution.
      
      

    • Ehrlichiosis monocítica canina en la provincia de Entre Ríos, Argentina: confirmación molecular de casos en la ciudad de Concordia. (Completo, 2020)

      Sánchez, R.O. , Bazzano, V. , FÉLIX, M.L. , ARMUA-FERNANDEZ, M. T. , VENZAL, J.M.
      FAVE Sección Ciencias Veterinarias, v.: 19 p.:16 - 22, 2020
      Palabras clave: ehrlichiosis monocítica canina Ehrlichia canis Rhipicephalus sanguineus trombocitopenia Argentina
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
      Medio de divulgación: Internet
      ISSN: 1666938X
      E-ISSN: 23625589
      DOI: doi: https://doi.org/10.14409/favecv.v19i1.8935
      El objetivo del siguiente trabajo fue confirmar la presencia de Ehrlichia canis por diagnóstico molecular, a partir de muestras de perros con diagnósticos presuntivos, oriundos de la ciudad de Concordia, provincia de Entre Ríos. ADN fue extraído de 14 muestras de sangre de perros con diagnóstico presuntivo de EMC. Para la detección de ADN de E. canis se amplificó un fragmento del gen dsb, específico de este género, y su confirmación específica se realizó mediante secuenciación. Doce de las 14 muestras mostraron bandas del tamaño esperado. Las secuencias obtenidas mostraron un 98-100% de identidad con secuencias registradas en GenBank para E. canis. Además, se amplificó un fragmento del gen ARNr 16S mitocondrial de garrapatas obtenidas de un perro positivo. Las secuencias demostraron que corresponden a R. sanguineus sensu stricto (linaje templado). En este estudio se confirma la presencia de E. canis en la ciudad de Concordia, Entre Ríos, resultando en una alerta para los médicos veterinarios de la región, a fin de incentivar estrategias de prevención de la enfermedad y control del vector.
      
      

    • First detection of ?Candidatus Rickettsia wissemanii? in Ornithodoros hasei (Schulze, 1935) (Acari: Argasidae) from Argentina (Completo, 2020)

      Colombo, V.C. , Montani, M.E. , Pavé, R. , Antoniazzi, L.R. , Gamboa, M.D. , Fasano, A.A. , FÉLIX, M.L. , Nava, S. , VENZAL, J.M.
      Ticks and Tick-borne Diseases, v.: 11 p.:10144 - 10144, 2020
      Palabras clave: ?Candidatus Rickettsia wissemanii? Chiroptera Ornithodoros hasei Argentina
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: https://doi.org/10.1016/j.ttbdis.2020.101442
      The aim of this study was to assess the presence of Rickettsia in soft ticks (Acari: Argasidae) collected from insectivorous bats (Chiroptera) in Santa Fe province, Argentina. First, a subset of ticks were mounted in Hoyer?s medium to be determined by morphological characters and then confirmed by sequencing the mitochondrial 16S rRNA gene. Also ticks were processed by PCR assays using primers CS-78 and CS-323, which amplify a fragment of the Rickettsia spp. gltA gene. Positive ticks were subjected to a second PCR round with primers Rr190.70p and Rr190.701n of the spotted fever group rickettsiae ompA gene. A phylogenetic analysis was performed with Maximum-likelihood method, and the best fitting substitution models were determined with the Akaike Information Criterion. Five bats of the species Eptesicus diminutus Osgood, 1915, Eptesicus furinalis (d?Orbigny and Gervais, 1847), Eptesicus spp. (Vespertilionidae), and Molossops temminckii Burmeister, 1854 (Molossidae) were parasitized with Ornithodoros hasei (Schulze, 1935) larvae. One E. diminutus ticks? tested positive to ?Candidatus Rickettsia wissemanii?, a spotted fever group rickettsiae. The association O. hasei ??Ca. R. wissemanii? detected in this study represents the first evidence of a Rickettsia in Ornithodoros ticks in Argentina and the third report of this association in America. Also, this finding constitutes the first record of ?Ca. R. wissemanii? in Argentina. Finally, we found for the first time the insectivorous bats E. diminutus and E. furinalis parasitized with O. hasei larvae. These findings add two new hosts and a new location, the southernmost recorded to date, for O. hasei.
      
      

    • New records and phylogenetic position of Ornithodoros knoxjonesi (Ixodida: Argasidae). (Completo, 2020)

      Guzmán-Cornejo, C. , Rebollo-Hernández, A. , Troyo, A. , Moreira-Soto, R.D. , Hernández, L.V. , Muñoz-Leal, S. , Labruna, M.B. , Nava, S. , VENZAL, J.M.
      Ticks and Tick-borne Diseases, v.: 11 p.:10147 - 10147, 2020
      Palabras clave: Ticks Argasids Ornithodoros Neotropical region Bats
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: https://doi.org/10.1016/j.ttbdis.2020.101473
      Larvae of Ornithodoros knoxjonesi collected at five localities in three countries were studied using morphological and molecular methods to confirm this species? taxonomic validity. The larva of O. knoxjonesi is characterized as having 14 pairs of dorsal setae, eight pairs of ventral setae, plus a posteromedian seta; an elongate dorsal plate, tapered anteriorly; and a hypostome that is narrower near its midlength, with posteriorly projecting denticles. Although the larvae of O. knoxjonesi and Ornithodoros peropteryx are morphologically quite similar, the larva of O. knoxjonesi is characterized as having dorsal setae that are wider at the tip than at the base, while in O. peropteryx these setae are narrower at the tip than at the base; moreover, the dorsal setae are shorter in O. knoxjonesi (Al 0.037?0.065; Pl 0.035?0.059) than in O. peropteryx (Al 0.120?0.132; Pl 0.080?0.096). These species also differ in that O. knoxjonesi possesses only the Al seta on tarsus I, whereas O. peropteryx has both Al and Pl setae. And while both species have two setae on coxae I-III, in O. knoxjonesi the anterior seta is tapering and smooth and the posterior is fringed, while both setae are fringed in O. peropteryx. At the molecular level, based on a maximum likelihood analysis using approximately 400 bp of the mitochondrial 16S rDNA gene, O. knoxjonesi appears as an independent lineage, separated from O. peropteryx by a genetic distance of 16.28 %. Balantiopteryx plicata is a common host of O. knoxjonesi; however, in this work we report Pteronotus personatus and Pteronotus gymnonotus as new hosts of this tick species.
      
      

    • Detection of a putative novel genotype of Anaplasma in gray-brocket deer (Mazama gouazoubira) from Uruguay (Completo, 2020)

      FÉLIX, M.L. , ARMUA-FERNANDEZ, M. T. , Parodi, P. , Bazzano, V. , Mangold, A.J. , VENZAL, J.M.
      Experimental and Applied Acarology, v.: 81 p.:575 - 583, 2020
      Palabras clave: Anaplasmataceae Anaplasma sp. Mazama gouazoubira Ticks Uruguay
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
      Medio de divulgación: Internet
      ISSN: 01688162
      E-ISSN: 15729702
      DOI: https://doi.org/10.1007/s10493-020-00523-0
      Anaplasmataceae includes the genera Anaplasma, Ehrlichia, Neorickettsia and Wolbachia, comprising a group of obligate intracellular bacteria. The genus Anaplasma has pathogenic species transmitted by ticks of veterinary and human health importance. Wild ungulates such as deer represent important reservoirs and amplifiers of Anaplasmataceae. The interaction between deer and domestic ruminants represents a serious problem due to the transmission of these pathogens through their ectoparasites. In the present study, we investigated the presence of Anaplasmataceae organisms in blood, tissues and tick samples of a gray-brocket deer (Mazama gouazoubira). The specimen was found dead in a farm in northeast Uruguay. PCRs targeting partial regions of 16S rRNA and groESL genes were carried out for Anaplasmataceae DNA detection. Moreover, several ectoparasites were identified: the chewing louse Tricholipeurus albimarginatus, the Neotropical deer louse fly Lipoptena mazamae, and the ticks Haemaphysalis juxtakochi and Rhipicephalus microplus. A consensus sequence of 1274 bp of 16S rRNA was generated for Anaplasma sp. from the M. gouazoubira blood sample. All ticks analysed by PCR assays were negative. No band was detected in any of the samples after PCR targeting groESL gene. Phylogenetic analysis using 16S rRNA partial gene sequences, clustered the putative novel genotype sequence obtained in this study, named Anaplasma sp. genotype Mazama?Uruguay, along with Anaplasma sp. detected in Mazama sp., Mazama americana and Mazama bororo, all deer species from Brazil. Furthermore, this cluster showed to be closely related to Anaplasma bovis sequences obtained from various ruminants and other mammals from several parts of the world. The pathogenicity as well as its infecting potential to other cervids or domestic ruminants is currently unknown. Further studies should be performed in order to characterize this novel species, especially targeting other genes.
      
      

    • Molecular detection of rickettsial bacteria in ticks of the genus Ixodes from the Southern Cone of America (Completo, 2020)

      Sebastian, P.S. , Flores, F.S. , Saracho-Bottero, M.N. , Tarragona, E.L. , VENZAL, J.M. , Nava, S.
      Acta Tropica, v.: 210 p.:10558 - 10558, 2020
      Palabras clave: Ixodes Rickettsia Argentina Ca. Rickettsia mendelii Ticks Tick-borne rickettsiosis
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
      Medio de divulgación: Internet
      ISSN: 0001706X
      DOI: https://doi.org/10.1016/j.actatropica.2020.105588
      To obtain information about rickettsial bacteria in ticks of the genus Ixodes from Argentina and Uruguay, specimens of I. fuscipes (previously named as I. aragaoi), Ixodes pararicinus, Ixodes sp. cf. I. affinis, and Ixodes sp. were tested targeting the rickettsial gltA and ompA genes. Rickettsial bacteria was detected in all of these species. Rickettsia found in Ixodes sp. is closely related to Ca. Rickettsia mendelii, which was previously detected in I. ricinus of the Czech Republic and later in I. brunneus from the USA. Phylogenetic analyses of the Rickettsia strains found in I. fuscipes, I. pararicinus, and Ixodes sp. cf. I. affinis showed that these strains form a clade together with R. buchneri detected in I. scapularis from the USA, which is closely related to R. monacensis from Europe. Rickettsia buchneri, R. cooleyi and the Rickettsia detected in I. fuscipes, I. pararicinus, and Ixodes sp. cf. I. affinis clustered together in a clade well supported, which suggest that they are different strains of R. buchneri. The phylogenetic analysis shows that Ixodes ticks that are closely related in evolutionary terms (i.e. Ixodes species from the I. ricinus complex, I. brunneus Ixodes sp.) share closely related rickettsial strains. The results of this study show that rickettsial bacteria are present in Ixodes ticks from Argentina and describe the first detection of Ca. R. mendelii in South America.
      
      

    • A novel Ehrlichia strain (Rickettsiales: Anaplasmataceae) detected in Amblyomma triste (Acari: Ixodidae), a tick species of public health importance in the Southern Cone of America. (Completo, 2020)

      Cicuttin, G.L. , De Salvo, M.N. , Díaz Pérez, P. , Silva, D. , FÉLIX, M.L. , VENZAL, J.M. , Nava, S.
      Pathogens and Global Health, v.: 114 6 , p.:318 - 322, 2020
      Palabras clave: Ehrlichia ticks Amblyomma South America
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Ciencias de la Salud / Zoonosis
      Medio de divulgación: Internet
      ISSN: 20477724
      E-ISSN: 20477732
      DOI: https://doi.org/10.1080/20477724.2020.1795579
      The aim of this work was to report the detection of a putative novel Ehrlichia strain associated with the tick Amblyomma triste. Free-living adult ticks determined as A. triste were collected by drag-sampling in Argentina and Uruguay. Molecular detection of Ehrlichia agents was performed targeting three different loci: 16S rRNA gene, dsb gene and a fragment of groESL heat shock operon. In total, 164 adults of A. triste (38 from INTA E.E.A Delta del Paraná in Argentina and 126 from Toledo Chico in Uruguay) were analyzed. One tick (0.6%) collected in INTA E.E.A. Delta del Paraná (Argentina) was positive. The phylogenetic analyses show that the Ehrlichia strain found in this study (named Ehrlichia sp. strain Delta) represents an independent lineage within the genus Ehrlichia, close to E. chaffeensis and E. muris. This is also the first report of an Ehrlichia agent infecting the tick A. triste. The medical and veterinary significance of Ehrlichia sp. strain Delta remains to be demonstrated. However, it is important to mention that adults of A. triste are aggressive to humans and domestic mammals. Therefore, the potential role of A. triste in the transmission of Ehrlichia agents to humans or domestic animals across its distributional range should be highlighted, even more considering that Ehrlichia sp. strain Delta is phylogenetically related to the zoonotic E. chaffeensis, which is recognized as pathogenic to both humans and animals.
      
      

    • Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina (Completo, 2020)

      Flores, F.S. , Saracho-Bottero, M.N. , Sebastian, P.S. , Mangold, A.J. , VENZAL, J.M. , Nava, S.
      Ticks and Tick-borne Diseases, v.: 11 p.:10154 - 10154, 2020
      Palabras clave: Ticks Borrelia Ixodes ricinus complex Argentina
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Ciencias de la Salud / Zoonosis
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: https://doi.org/10.1016/j.ttbdis.2020.101546
      The aim of this work was to evaluate the presence of Borrelia infection in Ixodes sp. cf. Ixodes affinis ticks from Argentina. Specimens of Ixodes sp. cf. I. affinis were collected on vegetation and birds in five locations belonging the most humid part of the Chaco Biogeographic Province. Specimens were tested for Borrelia infection by nested-PCR targeting the flaB gene and the rrfA-rrlB intergenic spacer region (IGS), sequenced and phylogenetically analyzed. A total of 48 Ixodes sp. cf. I. affinis (12 questing adults from vegetation and 20 nymphs and 16 larvae on nine bird species: Arremon flavirostris, Basileuterus culicivorus, Campylorhamphus trochilirostris, Myiothlypis leucoblephara, Tachyphonus rufus, Thlypopsis sordida, Turdus amaurochalinus, Turdus rufiventris and Troglodytes aedon) were collected. Twelve adults, 14 nymphs and 11 larvae (3 individually and 8 in 3 pools) were analyzed. Partial sequences were detected in 6 adults, 11 nymphs and 4 larvae (2 individual and 2 pools). Phylogenetically, the Borrelia found in Ixodes sp. cf. I. affinis belongs to the B. burgdorferi sensu lato (s. l.) complex. The partial sequences obtained from the borrelian gene flaB and IGS were associated to two groups formed by sequences previously detected in Ixodes fuscipes, Ixodes longiscutatus and Ixodes pararicinus from the Southern Cone of America in northern Argentina, southern Brazil and Uruguay. The results of this work suggest that the haplotypes of B. burgdorferi s. l. complex detected in the three species of the I. ricinus complex distributed in the Southern Cone of America are related and widely distributed.
      
      

    • Confirmación de Argas neghmei (Ixodida: Argasidae) en Perú y reporte del carpintero andino (Colaptes rupicola) como nuevo hospedero. (Completo, 2020)

      Gomez-Puerta, L.A. , Muñoz-Leal, S. , Labruna, M.B. , VENZAL, J.M.
      Revista Peruana de Biología, v.: 27 4 , p.:533 - 536, 2020
      Palabras clave: Argasidae; Argas neghmei; Garrapatas blandas; carpintero andino; Colaptes rupicola; Puno
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 17279933
      DOI: http://dx.doi.org/10.15381/rpb.v27i4.19202
      Este informe describe el hallazgo de garrapatas blandas (Ixodida, Argasidae) parasitando a dos carpinteros andinos (Colaptes rupicola) provenientes del distrito de Nuñoa, provincia de Melgar en Puno, Perú (14°31?11.77"S; 70°32?15.95"W; 3967 m de altitud). Un total de 29 larvas de garrapatas fueron colectadas directamente de las aves y posteriormente identificadas morfológicamente como Argas neghmei. El diagnóstico fue basado en la morfología de las larvas y confirmado mediante el análisis molecular del gen 16S ARNr mitocondrial de la garrapata. Un fragmento de 363 pares de bases de este gen mostró una identidad del 100% con secuencias registradas para A. neghmei de Argentina y Chile (GenBank: FJ853598 y DQ295781). Este hallazgo confirma la presencia de A. neghmei en Perú y agrega al carpintero andino como nuevo hospedero para esta garrapata.
      
      

    • Tick species diversity and population dynamics of Ixodes ricinus in Galicia (north-western Spain). (Completo, 2019)

      Remesar, S. , Díaz, P. , VENZAL, J.M. , Pérez-Creo, A. , Prieto, A. , Estrada Peña, A , López, C.M. , Panadero, R. , Fernández, G. , Díez-Baños, P. , Morrondo, P.
      Ticks and Tick-borne Diseases, v.: 10 p.:132 - 137, 2019
      Palabras clave: Ixodes ricinus Questing ticks Phenology North-western Spain
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología / Acarología
      Medio de divulgación: Internet
      E-ISSN: 18779603
      DOI: https://doi.org/10.1016/j.ttbdis.2018.09.006
      Ticks are the major arthropod vectors of animal and human pathogens in Europe; thus, information of tick species and their phenology allows identifying those areas and periods involving a high risk of exposure to particular tick-borne pathogens. In order to assess the diversity of exophilic tick species in north western Spain, questing ticks were collected by flagging in 17 different locations; ticks were microscopically and molecularly identified at the 16S rDNA loci. In addition, phenology of questing ticks in the same region was studied in representative sampling areas of three different ecological regions, where ticks were monthly collected in 300-m transects during two complete years. The possible influence of different climate variables on the normalized accumulated fraction of nymphal and adult Ixodes ricinus was assessed using a generalized linear mixed model (GLMN). I. ricinus was the most frequently and widely distributed tick species; Dermacentor marginatus, Dermacentor reticulatus, Ixodes frontalis and Ixodes acuminatus were sporadically found. Nymphs were the most commonly collected stage. Both nymphs and adults of I. ricinus presented a unimodal activity pattern; density showed a peak in late spring early summer whereas the lowest levels were found at late summer-early autumn. GLMN showed that both nymphal and adult I. ricinus phenology was directly associated with the accumulated temperature, photoperiod and average daily maximum temperature and inversely associated with average daily maximum relative humidity. This information could be used to predict the annual periods associated with a higher exposure and risk of transmission of pathogens and to complete the global map of distribution of tick species.
      
      

    • Occurrence of Borrelia and Borreliella species in Ixodes ricinus collected from roe deer in northwestern Spain. (Completo, 2019)

      Díaz, P. , Remesar, S. , VENZAL, J.M. , Vázquez-López, M.A. , Fernández, G. , López, C. , Díez-Baños, P. , Morrondo, P. , Panadero, S.
      Medical and Veterinary Entomology, v.: 33 p.:427 - 430, 2019
      Palabras clave: Borreliella spp. Borrelia miyamotoi Ixodes ricinus roe deer northwest Spain
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Ciencias de la Salud / Parasitología
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 13652915
      DOI: 10.1111/mve.12364
      Ixodes ricinus, comprising the predominant tick species in Europe, can transmit important human pathogens, including Borreliella spp., the causal agent of Lyme borreliosis. One hundred and seventy five roe deer hunted in two areas (plateau and mountain) of Galicia (northwest Spain) were examined for the presence of ticks; all roe deer were infested by I. ricinus. Nymphs (n=1000), males (n=1449) and females (n=1000) of I. ricinus were analysed in pools of up to 10 ticks to detect both Borreliella and Borrelia DNA. The average number of I. ricinus per roe deer was similar in both areas, regardless of the life stage; although the percentage of Borreliella and Borrelia positive pools was higher in ticks collected from roe deer hunted in the plateau area, no significant differences were detected. Sequence analysis at the flagellin gene allowed the identification of four Borreliella species (Borreliella afzelii, Borreliella garinii, Borreliella lusitaniae and Borreliella valaisiana) and Borrelia miyamotoi in adult males; only B. valaisiana and B. miyamotoi were detected in nymphs and all females were negative. All Borreliella and Borrelia species found in roe deer were previously identified in questing I. ricinus collected in the same study area, although the prevalence was lower in the present study. The analysis of male I. ricinus ticks collected from roe deer gives a good estimation of Borreliella diversity in questing ticks.
      
      

    • Description of Ornithodoros montensis n. sp. (Acari, Ixodida: Argasidae), a parasite of the toad Rhinella arenarum (Amphibia, Anura: Bufonidae) in the Monte Desert of Argentina. (Completo, 2019)

      VENZAL, J.M. , Castillo, G.N. , Gonzalez-Rivas, C.J. , MANGOLD, A.J. , Nava, S.
      Experimental and Applied Acarology, v.: 78 p.:133 - 147, 2019
      Palabras clave: Ticks Argasidae Ornithodoros New species Argentina
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Acarología
      Medio de divulgación: Internet
      E-ISSN: 15729702
      DOI: https://doi.org/10.1007/s10493-019-00372-6
      A new tick species of the genus Ornithodoros (Acari: Argasidae) was described from larvae collected on the toad Rhinella arenarum in a locality from Argentina belonging to the Monte Biogeographic Province. Ornithodoros montensis n. sp. was described based on morphological traits and sequences of the mitochondrial 16S rRNA gene. The diagnostic characters for this species are a combination of idiosoma oval, dorsal plate pyriform with posterior margin slightly concave, dorsal surface with 17 pairs of setae (7 anterolateral, 4 to 5 central and 5 to 6 posterolateral), ventral surface with 6 pairs of setae and 1 pair on anal valves, three pairs of sternal setae, postcoxal setae absent, and hypostome pointed apically with dental formula 3/3 in the anterior half and 2/2 posteriorly almost to base. Phylogenetic analysis of 16S rDNA sequences and a principal component analysis based on morphometric characters provided additional support to the description of O. montensis as an independent lineage within the genus Ornithodoros. Larvae of O. montensis are phylogenetically closely related to O. puertoricensis, O. rioplatensis, O. talaje s.s., O. guaporensis, O. hasei and O. atacamensis, all of them belonging to the ?O. talaje group?.
      
      

    • Primer reporte de Haemaphysalis juxtakochi Cooley, 1946 (Acari: Ixodidae) parasitando equinos en Uruguay. (Completo, 2019)

      FÉLIX, M.L. , VENZAL, J.M.
      FAVE Sección Ciencias Veterinarias, v.: 18 p.:9 - 11, 2019
      Palabras clave: Ixodidae Haemaphysalis juxtakochi Uruguay equinos
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 1666938X
      E-ISSN: 23625589
      DOI: https://doi.org/10.14409/ favecv.v18i1.8141
      Se presenta el primer reporte de Haemaphysalis juxtakochi Cooley, 1946 parasitando equinos en Uruguay. Adultos de esta especie de garrapata fueron hallados en un equino junto a Rhipicephalus microplus (Canestrini, 1888) en una localidad del departamento de Tacuarembó, con presencia de monte nativo denso y abundante fauna silvestre, como el ciervo guazubirá (Mazama gouazoubira), hospedador preferencial de adultos de H. juxtakochi. Con este hallazgo son cuatro las especies de garrapatas parásitas de equinos reportadas para Uruguay: R. microplus, Amblyomma tigrinum Koch, 1844, Amblyomma triste Koch, 1844 y H. juxtakochi. La importancia médico-veterinario de H. juxtakochi en equinos es desconocida.
      
      

    • Leishmaniosis: breve puesta al día. (Completo, 2019)

      ARMUA-FERNANDEZ, M. T. , VENZAL, J.M.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 211 5 , p.:29 - 36, 2019
      Palabras clave: Leishmaniosis visceral Uruguay Lutzomyia longipalpis Leishmania infantum
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Escrito por invitación
      ISSN: 03764362
      E-ISSN: 16884809
      DOI: 10.29155/VET.55.211.5
      La leishmaniosis es un complejo de enfermedades, causadas por protozoarios parásitos del género Leishmania. Las presentaciones más típicas son: leishmaniosis cutánea (LC), leishmaniosis mucocutánea (LMC), y leishmaniosis visceral (LV). Las lesiones y la progresión de cada enfermedad están dadas por la especie de Leishmania. Estos parásitos son transmitidos por dípteros (flebotomíneos). Se han identificado a la fecha, varios géneros de flebotomíneos, siendo, Phlebotomus (viejo mundo) y Lutzomyia (nuevo mundo) los más importantes para la transmisión de Leishmania spp. Algunas de las especies de Leishmania son exclusivas del viejo mundo, y otras del continente Americano. Sin embargo, la especie más cosmopolita es L. infanutm, uno de los agente etiológicos más importantes de leishmaniosis visceral, ya que puede ser transmitida tanto por flebotomíneos del viejo como del nuevo mundo. El ciclo de L. infantum involucra a un vector biológico (Lutzomyia longipalpis) y un hospedero vertebrado (en nuestra región, el perro). Tal cual ocurre en humanos, la LV en perros es una enfermedad crónica y multisistémica. El espectro y gravedad de los síntomas varía ampliamente, pudiéndose encontrar perros poli, oligo o asintomáticos. El cometido de esta revisión bibliográfica es abordar diferentes aspectos sobre la epidemiología y control, tanto de Leishmania infantum como de su vector principal, Lu. longipalpis.
      
      

    • Longitudinal Study of Infection with Borrelia spp. in Questing Ticks from North-Western Spain. (Completo, 2019)

      Remesar, S. , Díaz, P. , VENZAL, J.M. , Prieto, A. , Estrada-Peña, A. , López, C.M. , Panadero, R. , Fernández, G. , Díez-Baños, P. , Morrondo, P.
      Vector-Borne and Zoonotic Diseases, v.: 19 11 , p.:785 - 792, 2019
      Palabras clave: Borrelia burgdorferi s.l. Borrelia miyamotoi Ixodes ricinus longitudinal study north-western Spain
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Ciencias de la Salud / Parasitología
      Medio de divulgación: Internet
      ISSN: 15303667
      E-ISSN: 15577759
      DOI: 10.1089/vbz.2019.2442
      Introduction: Borrelia burgdorferi sensu lato (s.l.), the causative agent of Lyme borreliosis, is the most prevalent tick-borne pathogen in Europe, where it is mainly transmitted by Ixodes ricinus. This tick also circulates Borrelia miyamotoi, a member of the relapsing fever group of species. Objectives: A longitudinal study was performed to assess the prevalence of Borrelia spp. In questing ticks. Relationships between Borrelia prevalence in ticks and some variables such as tick development stage, sampling area, and questing tick density were assessed; in addition, the distribution of B. burgdorferi s.l. was analyzed to establish the periods of acarological risk. Methods: Ticks were collected monthly by flagging in three different ecological areas (coast, plateau, and mountain) in north-western Spain during a 2-year study. Borrelia DNA was detected by PCR, targeting the flagellin (fla) gene. Positive samples were also characterized at the rrfA-rrlB intergenic spacer region and the glycerophosphodiester phosphodiesterase (GlpQ) gene. Results: B. burgdorferi s.l. DNA was detected in 11.84% of I. ricinus. Five Lyme Borrelia species were identified (Borrelia afzelii, B. burgdorferi sensu stricto, Borrelia garinii, Borrelia lusitaniae, and Borrelia valaisiana). One single relapsing fever species (B. miyamotoi) was detected (0.85%). Questing Ixodes frontalis, Ixodes acuminatus, Dermacentor reticulatus, and Dermacentor marginatus yield negative results. The prevalence of B. burgdorferi s.l. was significantly higher in female ticks and in the mountain area. In addition, a seasonal pattern in the B. burgdorferi s.l. prevalence distribution throughout the study was not detected. Conclusions: The detection of a noticeable prevalence of B. burgdorferi s.l. in questing I. ricinus suggests a high acarological risk, especially in mountain area. There is no evidence of a relationship between the prevalence of B. burgdorferi s.l. and the density of questing ticks, nor traces of a seasonal pattern in the values of prevalence in ticks.
      
      

    • Borrelia spp. in ticks and birds from a protected urban area in Buenos Aires city, Argentina (Completo, 2019)

      Cicuttin, G.L. , De Salvo, M.N. , Nava, S. , VENZAL, J.M.
      Ticks and Tick-borne Diseases, v.: 10 6 , p.:10128 - 10128, 2019
      Palabras clave: Borrelia ticks Buenos Aires city Argentina
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Ciencias de la Salud / Parasitología
      ISSN: 1877959X
      DOI: https://doi.org/10.1016/j.ttbdis.2019.101282
      This study was aimed to know epidemiological aspects of Borrelia spp. in a protected urban area of Buenos Aires city, Argentina, where thousands of people visit this area for recreational purposes. Ticks were collected from vegetation, birds and dogs. Three hundred and forty birds belonging to 43 species, 41 genera, 18 families and six orders were captured (90.3% corresponded to the order Passeriformes). One hundred and twenty ticks were collected from 47 birds (13.8%) belonging to 10 species (23.2%), all of them from to the order Passeriformes (Emberizidae, Furnariidae, Parulidae, Thraupidae, Troglodytidae, Turdidae). Ticks were identified as Ixodes auritulus (56 larvae, 44 nymphs and 8 females) and Amblyomma aureolatum (1 larva and 11 nymphs). One thousand and ninety-one ticks collected from vegetation, 100 ticks collected from birds, and 89 ticks from dogs were tested for Borrelia infection by PCR trials targeting the flagellin (fla) and 16S rRNA genes. In addition, 101 blood and 168 tissue samples from birds were analyzed. Nine nymphs of A. aureolatum (2.1%) and four nymphs of I. auritulus (0.7%) collected from vegetation were positive. Five nymphs of A. aureolatum (45.4%), and five pools of larvae (minimum infection rate 13.5%), 18 nymphs (40.9%) and one female (14.3%) of I. auritulus collected on birds were also positive. The remaining samples were negative. The phylogenetic tree generated with fla sequences shows that seven of the eight different haplotypes of Borrelia detected in I. auritulus conform an independent lineage within the Borrelia burgdorferi sensu lato complex together with sequences of Borrelia sp. detected in I. auritulus from Canada and Uruguay. The fla sequences of Borrelia obtained from A. aureolatum and one sequence of a single specimen of I. auritulus conform a phylogenetic group with Borrelia turcica, Borrelia sp. isolated from a tortoise in Zambia, Borrelia spp. detected in Amblyomma maculatum from USA and Amblyomma longirostre from Brazil. The epidemiological risk that implies the infection with Borrelia genospecies associated with I. auritulus seems to be low because this tick is not aggressive to humans, but it helps to maintain borrelial spirochetes in the enzootic transmission cycles. The pathogenicity to humans of the Borrelia found in A. aureolatum is unknown; however, adults of this tick species are known to bite humans. This is the first report of the presence of Borrelia in A. aureolatum. Further investigations are necessary to know the risk of transmission of borreliosis by hard ticks in the study area.
      
      

    • On the presence of Ornithodoros microlophi (Argasidae) in Peru (Completo, 2019)

      Muñoz-Leal, S. , NAVA, S. , González-Acuña, D. , Gomez-Puerta, L.A. , Labruna, M.B. , VENZAL, J.M.
      Systematic and Applied Acarology, v.: 24 11 , p.:2278 - 2284, 2019
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 13621971
      E-ISSN: 20566069
      DOI: 10.11158/saa.24.11.17
      
      

    • Species occurrence of ticks in South America, and interactions with biotic and abiotic traits (Completo, 2019)

      Estrada-Peña, A. , NAVA, S. , Tarragona, E. , Bermúdez, S. , de la Fuente, J. , Domingos, A. , Labruna, M.B. , Mosqueda, J. , Merino, O. , Szabó, M. , VENZAL, J.M. , Guglielmone, A.A.
      Scientific Data, v.: 6 1 , p.:299 - 299, 2019
      Palabras clave: Ana Domingos Marcelo Labruna Juan Mosqueda Octavio Merino Matias Szabó Jose M. Venzal Alberto A. Guglielmone.
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 20524463
      DOI: https://doi.org/10.1038/s41597-019-0314-0
      The datasets of records of the distribution of ticks and their hosts are invaluable tools to understand the phylogenetic patterns of evolution of ticks and the abiotic traits to which they are associated. Such datasets require an exhaustive collection of bibliographical references. In most cases, it is necessary the confirmation of reliable identification of ticks, together with an update of the scientific names of the vertebrate hosts. these data are not easily available, because many records were published in the so-called ?grey literature?. Herein, we introduced the Dataset of ticks in South America, a repository that collates data on 4,764 records of ticks (4,124 geo-referenced) with a special reference to an extra 2,370 records of ticks on cattle, together with a set of abiotic traits, curated from satellite-derived information over the complete target region. the dataset includes details of the phylogenetic relationships of the species of hosts, providing researchers with both biotic and abiotic traits that drive the distribution and evolution of ticks in South America.
      
      

    • The geographic distribution of Argas (Persicargas) miniatus and Argas (Persicargas) persicus (Acari: Argasidae) in America, with morphological and molecular diagnoses from Brazil, Chile and Cuba. (Completo, 2018)

      MUÑOZ-LEAL, S. , VENZAL, J.M. , NAVA, S. , REYES, M. , MARTINS, T.F. , LEITE, R.C. , VILELA, V.L.R. , BENATTI, H.R. , RíOS-ROSAS, D. , BARROS-BATTESTI, D.M. , GONZÁLEZ-ACUÑA, D. , LABRUNA, M.B.
      Ticks and Tick-borne Diseases, v.: 9 p.:44 - 56, 2018
      Palabras clave: SOUTH AMERICA Argas Soft ticks North America
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Acarología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: 10.1016/j.ttbdis.2017.10.009
      High similarity of morphological traits has historically overshadowed the identities and distributions of poultry associated soft ticks Argas (Persicargas) miniatus and Argas (Persicargas) persicus in America. In order to model the occurrence of both parasites in the continent, in the current study we performed morphological and molecular analyses to identify ticks collected in hen houses from Brazil and northern Chile. Combining these results with literature data, and the examination of Argas allotments deposited in the tick collections “Coleção Nacional de Carrapatos Danilo Gonçalves Saraiva” (Brazil), the “Coleção Acarológica do Instituto Butantan São Paulo” (Brazil), and the “Colección Zoológica de la Academia de Ciencia de Cuba” (Cuba), we present a critical list with the localities where A. (P.) miniatus and A. (P.) persicus have been reported in the American continent. Our results confirmed the presence of A. (P.) miniatus in Brazil and Cuba, and A. (P.) persicus in Chile, which in particular, constitutes the first molecularly confirmed report of the later species for South America. Although A. (P.) miniatus and A. (P.) persicus have been documented in 21 American countries, the identity of some reports must still be considered as uncertain until detailed morphological and/or molecular studies are performed. When contrasted to a Köppen-Geiger climate classification, A. (P.) miniatus predominantly occurs in equatorial and A. (P.) persicus in arid climates. However, until undetermined reports of both species are correctly identified, any conclusion on their geo-climatological occurrence throughout the American continent would be rather speculative.
      
      

    • Ticks infesting cattle and humans in the Yungas Biogeographic Province of Argentina, with notes on the presence of tick‑borne bacteria (Completo, 2018)

      SARACHO?BOTTERO, M.N. , TARRAGONA, E.L. , SEBASTIAN, P.S. , VENZAL, J.M. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Experimental and Applied Acarology, v.: 74 p.:107 - 116, 2018
      Palabras clave: Ixodidae Argentina Humans cattle Bacteria Yungas
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
      Medio de divulgación: Internet
      ISSN: 01688162
      E-ISSN: 15729702
      DOI: 10.1007/s10493-018-0208-4
      This study was performed to determine the tick species that infest cattle and humans throughout an altitudinal gradient in the Yungas Biogeographic Province of Argentina. The presence of tick-borne bacteria of the genera Rickettsia, Ehrlichia and Borrelia in the collected ticks was also evaluated. Samples of ticks parasitizing cattle and humans were carried out in different seasons. Questing ticks (adults and nymphs) were collected from vegetation and analyzed to detect the presence of Rickettsia, Ehrlichia and Borrelia by a battery of different PCRs. Five species of hard ticks were found parasitizing cattle: Amblyomma sculptum, Amblyomma tonelliae, Amblyomma hadanii, Haemaphysalis juxtakochi and Ixodes pararicinus. Amblyomma sculptum (immature and adults), A. tonelliae (immature and adults), A. hadanii (larvae) and one nymph of I. pararicinus were found attached to humans. Rickettsia amblyommatis was detected in one nymph of A. hadanii. DNA of a Borrelia genospecies belonging to the B. burgdorferi s.l. complex (phylogenetically related to haplotypes previously reported in Ixodes aragaoi from Uruguay and I. pararicinus from Argentina) was detected in adults of I. pararicinus. Amblyomma sculptum and I. pararicinus appear to be the tick species more frequent on cattle in the YBP from Argentina, and A. sculptum and A. tonelliae, were the main ticks found attached to humans. The medical importance of the bacteria of the genus Rickettsia and Borrelia detected in this work remains unknown.
      
      

    • An autochthonous confirmed case of Rickettsia parkeri rickettsiosis in Uruguay. (Completo, 2018)

      Faccini-Martínez, A.A. , FÉLIX, M.L. , ARMUA-FERNANDEZ, M. T. , VENZAL, J.M.
      Ticks and Tick-borne Diseases, v.: 9 p.:718 - 719, 2018
      Palabras clave: Rickettsia parkeri Eschar-associated rickettsiosis Tick-borne diseases Diagnosis Uruguay
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: 10.1016/j.ttbdis.2018.02.015
      Rickettsia parkeri, a member of the spotted fever group (SFG) rickettsiae, was first confirmed as an etiological agent of human rickettsiosis in 2004. Nearly all cases are characterized by an inoculation eschar, and no fatalities have been reported. In Uruguay, probable human cases of R. parkeri infection (confused initially with R. conorii infection) have been described since 1990 using the clinical name ?cutaneous-ganglionar? rickettsiosis. This is the only tick-borne rickettsiosis reported in the country. A single case of R. parkeri rickettsiosis has been confirmed by molecular and serological testing in a Spanish traveller returning from Uruguay. We report the first autochthonous human R. parkeri infection, confirmed by molecular testing in Uruguay.
      
      

    • Amblyomma yucumense Krawczak, Martins & Labruna, 2015 (Acari: Ixodidae) in Argentina. (Completo, 2018)

      Lamattina, D. , VENZAL, J.M. , Tarragona, E.L. , Mangold, A.J. , Nava, S.
      Systematic and Applied Acarology, v.: 23 5 , p.:799 - 802, 2018
      Palabras clave: Amblyomma yucumense Ixodidae Argentina
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 13621971
      E-ISSN: 20566069
      DOI: 10.11158/saa.23.5.2
      
      

    • The human flea Pulex irritans (Siphonaptera: Pulicidae) in northwestern Argentina, with an investigation of Bartonella and Rickettsia spp. (Completo, 2018)

      Lareschi, M. , VENZAL, J.M. , Nava, S. , Mangold, A.J. , Portillo, A. , Palomar-Urbina, A.M. , Oteo-Revuelta, J.A.
      Revista Mexicana de Biodiversidad, v.: 89 p.:375 - 381, 2018
      Palabras clave: Flea Siphonaptera Pulex Bacteria Pathogens Argentina
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Entomología
      Medio de divulgación: Internet
      ISSN: 18705081
      DOI: doi.org/10.22201/ib.20078706e.2018.2.2392
      Pulex irritans is the only cosmopolitan flea species and the most studied one within the genus Pulex. It has importance in public health since it commonly parasitizes humans causing dermatitis, and it has been also implicated in the transmission of bacterial pathogens. Pulex irritans has been confused with the closely related Pulex simulans species for years. Herein, Pulex specimens collected from a Pampas fox and a Chacoan peccary from northwestern Argentina were identified by comparison with type specimens. In addition, the presence of Bartonella spp. and Rickettsia spp. was investigated using PCR assays. Our results provided characters of diagnostic importance to identify P. irritans, which include the shape of sternite VII in the females, and of the aedeagal sclerite, clasper and crochet in the males. Besides, we report for the first time P. irritans parasitizing a peccary. This finding reinforces the hypothesis of the origin of this flea associated with this mammal, and then colonizing humans and domestic mammals. There was no evidence of Bartonella or Rickettsia DNA in the analysed fleas. This information even if negative may be considered relevant for P. irritans from Argentina.
      
      

    • Redescription, molecular features, and neotype deposition of Rhipicephalus pusillus Gil Collado and Ixodes ventalloi Gil Collado (Acari, Ixodidae). (Completo, 2018)

      Estrada-Peña, A. , VENZAL, J.M. , Nava, S.
      Zootaxa, v.: 4442 2 , p.:262 - 276, 2018
      Palabras clave: Rhipicephalus pusillus Ixodes ventalloi redescription neotypes 16rDNA
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Entomología
      Medio de divulgación: Internet
      ISSN: 11755326
      E-ISSN: 11755334
      DOI: https://doi.org/10.11646/zootaxa.4442.2.4
      Two species of ticks, Rhipicephalus pusillus Gil-Collado and Ixodes ventalloi Gil-Collado are redescribed, their molecular features (16S rDNA) compared with near species, and neotypes named and deposited. The male of R. pusillus is characterized by a smooth dorsal surface, with relatively short marginal grooves, not reaching the eyes and reaching the first festoon, longer than wide adanal plates, internally concave and widely rounded posteriorly, without internal spurs, accessory adanal plates not projecting over the cuticle, and a narrow, long and straight process on the spiracular plate. The female has very small porose areas, separated by 2.5-3 diameters, and a large triangular spur on the ventro-internal side of the palpal article 1. The nymph has lateral processes curved anteriorly in the ventral aspect of the basis capituli, long auriculae, and internal spurs on the four coxae. The larva of R. pusillus is separated by the presence of spurs on the ventral surface of palpal segment I, together with the rounded apices of the palpi. Features of 16S rDNA cluster this species near the R. sanguineus s.l. complex of species. The distinctive feature of the female of I. ventalloi is the long and curved au-riculae, which are absent in every other species of Ixodes reported in the Western Palaearctic. The male is characterized by relatively long conscutal setae and deep and well defined cervical fields. The nymph is unique by having slightly ap-parent lateral carinae and small cornua, very abundant and long alloscutal setae, about six times longer than those on the scutum and internal spur of coxa I reaching coxa II. The larva of I. ventalloi has Md1-Md3 setae of the same size as the rest of the conscutal setae, which are only slightly longer than the scutal setae, cornua present, and anal groove clearly divergent. In addition to the unique combination of morphological features, the molecular 16rDNA sequence clearly separates I. ventalloi from other species of the I. ricinus group. We named and deposited in an internationally accessible col-lection the neotypes of both species, since the holotypes were lost.
      
      

    • Molecular evidence of Babesia species in Procyon cancrivorus (Carnivora, Procyonidae) in Uruguay (Completo, 2018)

      Thompson, C.S. , Mangold, A.J. , Félix, M.L. , Carvalho, A. , Armúa-Fernández, M.T. , VENZAL, J.M.
      Veterinary Parasitology. Regional Studies and Reports, v.: 13 p.:230 - 233, 2018
      Palabras clave: Babesia sp. Crab-eating raccoon Procyon cancrivorus Amblyomma aureolatum Uruguay
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 24059390
      DOI: 10.1016/j.vprsr.2018.07.008
      The crab-eating raccoon (Procyon cancrivorus) is a carnivore widely distributed from southern Central America to all South American countries except Chile. In the Southern cone of America, P. cancrivorus has been found parasitized by several Amblyomma spp. Particularly, in Uruguay, A. aureolatum is the only tick found in this wild carnivore. Piroplasmid hemoparasites were found in Procyon lotor from North America and Japan. In this work, molecular evidence Babesia sp. DNA was found in blood and tissues from road-killed P. cancrivorus from different locations in Uruguay. PCRs targeting 18S rRNA gene were carried out. Subsequently, the obtained amplicones were sequenced and full-length sequences was assembled. A phylogenetic tree was constructed and revealed that the Babesia sp. found in this work clustered with other 18sRNA sequences of Babesia spp. obtained from P. lotor from Japan and USA, along with Babesia spp. of maned wolf and I. ovatus. This is the first report of molecular evidence of Babesia sp. parasitizing P. cancrivorus.
      
      

    • Ecological characterization of a tick community across a landscape gradient exhibiting differential anthropogenic disturbance in the Atlantic Forest ecoregion in Argentina (Completo, 2018)

      Lamattina, D. , VENZAL, J.M. , Costa, S.A. , Arrabal, J.P. , Flores, S. , Berrozpe, P.E. , Daniel González-Acuña , Guglielmone, A.A. , Nava, S.
      Medical and Veterinary Entomology, v.: 32 3 , p.:271 - 281, 2018
      Palabras clave: Ixodidae diversity environmental gradient host preferences lifecycle population ecology
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 0269283X
      E-ISSN: 13652915
      DOI: doi:10.1111/mve.12295
      An ecological analysis of a tick (Ixodida: Ixodidae) community across a landscape gradient presenting differential anthropogenic disturbance in the Atlantic Forest ecoregion in Argentina was performed. Ticks were collected from vegetation and hosts between September 2014 and August 2016. A total of 12 697 free-living ticks and 3347 specimens from hosts were collected, including 317 ticks infesting humans. The values obtained show considerable species diversity in the forest environment accompanied by low equitability. The similarity index derived from a comparison of forest and agricultural environments was higher than that calculated by comparing forest and urban environments. The data suggest that although a cycle of one generation per year is apparent in some species, more than one cohort may co-exist within the populations of some of these species. Well-marked patterns of the seasonal distribution of free-living tick species emerged in environments with no anthropic modification. The results indicate that forest environments are more suitable habitats than agricultural and urban environments for many species of native tick, but are unsuitable for exotic species that have successfully established in environments that have been modified by man.
      
      

    • The Nothoaspis amazoniensis Complete Mitogenome: A Comparative and Phylogenetic Analysis (Completo, 2018)

      Lima, P.H.C. , Vidigal, P.M.P. , Barcelos, R.M. , Klein, R.C. , Montandon, C.E. , Fabres-Klein, M.H. , Dergam, J.A. , VENZAL, J.M. , Mafra, C.
      Veterinary Sciences, v.: 5 37 , p.:1 - 10, 2018
      Palabras clave: Argasids Nothoaspis amazoniensis Ticks Phylogenomic taxonomic arrangement mitogenome
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Bioquímica y Biología Molecular /
      Medio de divulgación: Internet
      E-ISSN: 23067381
      DOI: doi:10.3390/vetsci5020037
      The molecular biology era, together with morphology, molecular phylogenetics, bioinformatics, and high-throughput sequencing technologies, improved the taxonomic identification of Argasidae family members, especially when considering specimens at different development stages, which remains a great difficulty for acarologists. These tools could provide important data and insights on the history and evolutionary relationships of argasids. To better understand these relationships, we sequenced and assembled the first complete mitochondrial genome of Nothoaspis amazoniensis. We used phylogenomics to identify the evolutionary history of this species of tick, comparing the data obtained with 26 complete mitochondrial sequences available in biological databases. The results demonstrated the absence of genetic rearrangements, high similarity and identity, and a close organizational link between the mitogenomes of N. amazoniensis and other argasids analyzed. In addition, the mitogenome had a monophyletic cladistic taxonomic arrangement, encompassed by representatives of the Afrotropical and Neotropical regions, with specific parasitism in bats, which may be indicative of an evolutionary process of cospeciation between vectors and the host.
      
      

    • Factors associated with hard tick (Acari: Ixodidae) parasitism in medium sized mammals in the Atlantic Rainforest region of Argentina. (Completo, 2018)

      Lamattina, D. , Costa, S.A. , Arrabal, J.P. , VENZAL, J.M. , Guglielmone, A.A. , Nava, S.
      Ticks and Tick-borne Diseases, v.: 9 p.:1451 - 1458, 2018
      Palabras clave: Ixodidae Nasua nasua Didelphis aurita Generalized linear models
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Ecología /
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: https://doi.org/10.1016/j.ttbdis.2018.06.009
      Associations with environmental and host parameters were assessed to describe tick parasitism patterns in two medium-sized mammals of the Atlantic rainforest region of Argentina. Ticks found on 93 specimens of Nasua nasua and 26 specimens of Didelphis aurita captured at six sites in the Iguazú National Park were collected. Generalized linear models were constructed to explain the presence and abundance of ticks and the most appropriate ones were selected after stepwise simplification. The season, site and host body mass variables were important to explain the abundance of Amblyomma coelebs nymphs, while site was important to describe larval abundance of this species. Season was the most important variable for larvae and nymphs of Haemaphysalis juxtakochi, while Amblyomma brasiliense nymphs were associated with host age. Amblyomma ovale larvae were related to host age, and adult ticks with host sex. The results indicate that these medium-sized mammals are important to support the immature tick stages and that both host and environmental factors may be associated with parasite loads.
      
      

    • Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization (Completo, 2018)

      Nava, S. , Beati, L. , VENZAL, J.M. , Labruna, M.B. , Szabó, M.P.J. , Petney, T. , Saracho-Bottero, M.N. , Tarragona, E.L. , Dantas-Torres, F. , Santos Silva, M.M. , Mangold, A.J. , Guglielmone, A.A. , Estrada-Peña, A.
      Ticks and Tick-borne Diseases, v.: 9 p.:1573 - 1585, 2018
      Palabras clave: Rhipicephalus sanguineus sensu stricto Neotype Morphological re-description DNA sequences
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Acarología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: https://doi.org/10.1016/j.ttbdis.2018.08.001
      The aims of this work were to re-describe all parasitic stages of Rhipicephalus sanguineus sensu stricto, to select and deposit a neotype, and to characterize some of its diagnostic molecular traits. A male of R. sanguineus s.s. collected in Montpellier, France, was designated as neotype. The diagnostic characters unique to the male of R. sanguineus s.s. are: spiracular plate elongated and subtriangular in shape with a dorsal prolongation narrow and usually visible dorsally, with the dorsal prolongation narrower than the width of the adjacent festoon; punctations of the scutum moderate in number and unequal in size; marginal groove conspicuous, deep and punctate; posteromedian groove distinct and elongated, and posterolateral grooves often sub-circular, shorter than posteromedian groove; adanal plates long, wide, and subtriangular in shape, with a clear concavity in its inner margin and posterior margin broadly rounded or truncated; accessory adanal plates with the posterior end pointed, narrower than the width of adjacent festoon. The female of R. sanguineus s.s. can be diagnosed by a combination of broadly U-shaped genital aperture, spiracular plate with a narrow dorsal prolongation visible dorsally, basis capituli hexagonal with broad lateral angles, and scutum barely longer than broad with posterior margin sinuous and punctations moderate in number and unequal in size, larger and more numerous along cervical fields. The nymph has a basis capituli sub-triangular dorsally with lateral angles slightly curved and presence of ventral processes, scutum approximately as long as broad with lateral margins nearly straights, posterior margin broadly rounded, and cervical grooves short and sigmoid in shape extending posteriorly to the level of the eyes. The larva is characterized by basis capituli broader than long with lateral angles short and slightly curved and with posterior margin slightly convex, cervical grooves short, shallow and subparallel, and scutum almost twice broader than long. The phylogenetic analysis of DNA sequences support R. sanguineus s.s. as a well-defined taxon when compared with other species of the R. sanguineus group: R. turanicus s.s., R. camicasi, R. guilhoni, R. sulcatus, R. pusillus, R. rossicus and R. leporis. Molecularly R. sanguineus s.s. also encompasses the so-called ?temperate lineage? from the New World (Argentina, southern Brazil, Chile, Uruguay, and USA). The evidence currently available supports the presence of R. sanguineus s.s. in Europe (France, Italy, Spain, Switzerland and Portugal) and America (Argentina, Brazil, Chile, Uruguay and U.S.A.), but further studies are needed to determine the exact geographic range of this taxon.
      
      

    • A morphological and phylogenetic analysis of Ornithodoros marinkellei (Acari: Argasidae), with additional notes on habitat and host usage. (Completo, 2018)

      VENZAL, J.M. , Nava, S. , Hernández, L.V. , Miranda, J. , Marcili, A. , Labruna, M.B.
      Experimental and Applied Acarology, v.: 76 p.:249 - 261, 2018
      Palabras clave: Ticks ? Bats ? Pteronotus ? Human parasitism ? Hot cave
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 15729702
      DOI: https://doi.org/10.1007/s10493-018-0307-2
      Ornithodoros marinkellei was described from larvae collected on Pteronotus spp. Bats in Colombia and Panama. More recently, this tick was reported in the Brazilian Amazon. Because some morphometric differences were observed between O. marinkellei larvae from Colombia and Brazil, it was proposed that further investigations were needed to assess whether the differences could be attributed to intra- or inter-specific polymorphism. Herein, we collected O. marinkellei specimens in the type locality of Colombia, in Brazil, and in a new locality in Nicaragua, expanding the distribution of the species to Nicaragua. Morphometric analysis of larvae and adults, corroborated by a principal component analysis (PCA), indicated that the Brazilian specimens were larger than specimens from Colombia and Nicaragua. Phylogenetic analysis inferred from the mitochondrial 16S rRNA gene showed ticks from Colombia and Nicaragua more genetically related than any of them with ticks from Brazil, although ticks from the three countries grouped in a clade sister to a major clade containing sequences of various Neotropical Ornithodoros species. We concluded that ticks identified as O. marinkellei from Colombia, Nicaragua, and Brazil represent the same taxon, and that the genetic and morphological differences between them are likely to have a geographical bias. We redescribed the nymph of O. marinkellei, which has a vestigial hypostome, probably incompatible with blood feeding. We also report human infestation by O. marinkellei adults. As all reports of O. marinkellei adults have been from hot caves (temperature > 35 °C), this abiotic condition could be a limiting factor for the occurrence of this tick species.
      
      

    • Diagnóstico parasitológico y molecular de tripanosomiasis canina por Trypanosoma evansi en perros asociados a la caza en dos localidades del Departamento de Artigas, Uruguay. (Completo, 2018)

      Parodi, P. , Freire, J. , Armúa-Fernández, M.T. , FÉLIX, M.L. , Guerisoli, D. , Bazzano. V. , Carvalho, L. , VENZAL, J.M.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 210 5 , p.:29 - 35, 2018
      Palabras clave: Tripanosomiasis canina surra anemia Trypanosoma evansi Uruguay
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 03764362
      E-ISSN: 16884809
      DOI: 10.29155/VET.54.209.5
      El objetivo del presente trabajo es reportar dos casos autóctonos fatales de tripanosomiasis canina causada por Trypanosoma evansi, enfermedad también conocida como surra, en perros cruza utilizados para la caza provenientes de dos localidades del Departamento de Artigas, Uruguay. Los casos se presentaron en los meses de setiembre y noviembre del 2017. Los signos clínicos fueron anemia, edemas en zona ocular, oral y miembros posteriores, inapetencia y desmejoramiento general progresivo. El diagnóstico se realizó tomando en cuenta signos clínicos y análisis paraclínicos: hemograma, funcional hepático, frotis de sangre y PCR/secuenciación. En los frotis sanguíneos se visualizaron tripomastigotes cuya morfología era compatible con Trypanosoma spp. En hemograma se destaca anemia hipocrómica y trombocitopenia, en el funcional hepático se detectaron altos niveles de enzimas hepáticas (AST/GGT/GOT/Fosfatasa Alcalina). Se realizaron tres PCRs que amplificaron un fragmento parcial del gen ARN ribosomal 18S (ARNr 18S) así como de dos fragmentos solapantes del espaciador transcrito interno ITS (ITS1+5,8S+ITS2). Las secuencias obtenidas tuvieron una homología del 100% para el gen ARNr 18S y del 99% para la región ITS con T. evansi. Estos casos representan el segundo diagnóstico para Uruguay de T. evansi y los primeros reportes de muerte a causa de esta tripanosomiasis en perros para el país. Se trata de una enfermedad a tener en cuenta ya que además de considerarse emergente para el país puede afectar gravemente a otras especies, siendo de notificación a la OIE (Organización Mundial de Sanidad Animal).
      
      

    • The Amblyomma maculatum Koch, 1844 (Acari: Ixodidae) group of ticks: phenotypic plasticity or incipient speciation? (Completo, 2018)

      Mendoza-Uribe, L. , Caceres, A.G. , Delgado-de la Mora, J. , Licona-Enriquez, J.D. , Delgado-de la Mora, D. , Labruna, M.B. , Durden, L.A. , Allerdice, M.E.J. , Paddock, C.D. , Szabó, M.P.J. , VENZAL, J.M. , Guglielmone, A.A. , Beati, L.
      Parasites & Vectors, v.: 11 p.:610 - 610, 2018
      Palabras clave: Amblyomma Systematics Ticks Amblyomma maculatum Taxonomic reassessment Phylogenetic analysis Amblyomma triste
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Acarología
      Ciencias Naturales y Exactas / Ciencias Biológicas / Biología y Biología de la Evolución /
      Medio de divulgación: Internet
      E-ISSN: 17563305
      DOI: doi.org/10.1186/s13071-018-3186-9
      Background: The goal of this study was to reassess the taxonomic status of A. maculatum, A. triste and A. tigrinum by phylogenetic analysis of five molecular markers [four mitochondrial: 12S rDNA, 16S rDNA, the control region (DL) and cytochrome c oxidase 1 (cox1), and one nuclear: ribosomal intergenic transcribed spacer 2 (ITS2)]. In addition, the phenotypic diversity of adult ticks identified as A. maculatum and A. triste from geographically distinct populations was thoroughly re-examined. Results: Microscopic examination identified four putative morphotypes distinguishable by disjunct geographical ranges, but very scant fixed characters. Analysis of the separated mitochondrial datasets mostly resulted in conflicting tree topologies. Nuclear gene sequences were almost identical throughout the geographical ranges of the two species, suggesting a very recent, almost explosive radiation of the terminal operational taxonomic units. Analysis of concatenated molecular datasets was more informative and indicated that, although genetically very close to the A. maculatum - A. triste lineage, A. tigrinum was a monophyletic separate entity. Within the A. maculatum - A. triste cluster, three main clades were supported. The two morphotypes, corresponding to the western North American and eastern North American populations, consistently grouped in a single monophyletic clade with many shared mitochondrial sequences among ticks of the two areas. Ticks from the two remaining morphotypes, south-eastern South America and Peruvian, corresponded to two distinct clades. Conclusions: Given the paucity of morphological characters, the minimal genetic distance separating morphotypes, and more importantly the fact that two morphotypes are genetically indistinguishable, our data suggest that A. maculatum and A. triste should be synonymized and that morphological differences merely reflect very recent local adaptation to distinct environments in taxa that might be undergoing the first steps of speciation but have yet to complete lineage sorting. Nonetheless, future investigations using more sensitive nuclear markers and/or crossbreeding experiments might reveal the occurrence of very rapid speciation events in this group of taxa. Tentative node dating revealed that the A. tigrinum and A. maculatum - A. triste clades split about 2 Mya, while the A. maculatum - A.triste cluster radiated no earlier than 700,000 years ago.
      
      

    • Procaudotestis cordiformis sp. nov. (Digenea: Apocreadiidae), parasite of Rhinelepis strigosa (Osteichthyes: Loricariidae) from Uruguay River basin, Uruguay. (Completo, 2018)

      Castro, O. , Félix, M.L. , VENZAL, J.M.
      Boletín de la Sociedad Zoológica del Uruguay, v.: 27 2 , p.:41 - 45, 2018
      Palabras clave: Procaudotestis cordiformis sp. nov. Apocreadiidae Rhinelepis strigosa Uruguay river basin.
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 02554402
      E-ISSN: 23936940
      Procaudotestis cordiformis sp. nov. (Digenea: Apocreadiidae) is described from specimens collected in the loricariid catfish Rhinelepis strigosa (Osteichthyes: Loricariidae) from Uruguay River basin, Uruguay. The new species is morphologically similar to the only species known of the genus, Procaudotestis uruguayensis Szidat, 1954. P. cordiformis is proposed for specimens with the following features: a heart-like body form, pharynx disproportionately larger, testes more anteriorly located, ovary and anterior testis with overlapping fields, vitellaria less extended in relation to body length and with fields not confluent posteriorly, and eggs wider than those described for P. uruguayensis. An amended diagnosis of the genus Procaudotestis is proposed.
      
      

    • Anaplasma platys in dogs from Uruguay (Completo, 2017)

      CARVALHO, L. , ARMÚA, M.T. , SOSA, N. , FÉLIX, M.L. , VENZAL, J.M.
      Ticks and Tick-borne Diseases, v.: 8 p.:241 - 245, 2017
      Palabras clave: Uruguay Rhipicephalus sanguineus dogs Anaplasmataceae Ehrlichia canis
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: 10.1016/j.ttbdis.2016.11.005
      Anaplasmataceae family members include vector-borne bacteria of veterinary importance that may alsoaffect humans. Ehrlichia canis and Anaplasma platys are the main members of this family detected in dogsworldwide. In Uruguay there are not many published studies on tick-borne pathogens affecting dogs,the only haemoparasite molecularly confirmed in dogs, is the piroplasm Rangelia vitalii. The aim of thepresent work was to detect the presence of A. platys and E. canis in dogs and dogs-associated ticks of twolocalities in Northwestern Uruguay. Blood samples from dogs with and without clinical signs associatedwith vector-borne diseases, and Rhipicephalus sanguineus obtained from these dogs were analyzed byPCR for Anaplasmataceae. Positive dogs were further analyzed by PCR for Ehrlichia spp. and A. platys. Allthe ticks were found negative. No dog was detected infected with E. canis, while eight dogs (4.2%) werefound to be infected with A. platys. Phylogenetic analysis of groESL operon sequence for A. platys revealedno differences with sequences described for A. platys in neighbor countries and from other regions of theworld. This is the first report of the presence of A. platys in Uruguay.
      
      

    • Molecular evidence and additional morphological characters to distinguish Ornithodoros brodyi and Ornithodoros yumatensis (Ixodida: Argasidae) in their different developmental stages (Completo, 2017)

      GUZMÁN-CORNEJO, C. , GARCíA-PRIETO, L. , REBOLLO-HERNáNDEZ, A. , VENZAL, J.M. , NAVA, S. , SáNCHEZ-MONTES, S.
      Acta Parasitologica, v.: 62 2 , p.:432 - 448, 2017
      Palabras clave: Ticks Morphology Mexico Argasids caves DNA
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Internet
      ISSN: 12302821
      E-ISSN: 18961851
      DOI: 10.1515/ap-2017-0051
      Ornithodoros brodyi and Ornithodoros yumatensis are two species distributed in the Americas and associated with bats and caves. Both species have similar morphology, and the diagnostic traits of adults have not been detailed or illustrated accurately. In this study, the independence of both species is validated on the basis of molecular evidence (using partial sequences of 16S rDNA gene), and the morphological differences between them (dentition of the hypostome and traits of individual mammillae) are confirmed through light and scanning electron microscopy. In addition to the above characteristics, we observed other traits that may serve to differentiate both species: dorsal setae are short and thick in O. yumatensis and are thin and moderate in size in O. brodyi. We also observed a conspicuous hood in O. brodyi, which was absent in O. yumatensis. Another characteristic observed is a line of setae, near the end of Tarsi II-IV, which in O. brodyi is formed by less than five setae and in O. yumatensis by more than five. The main morphological difference between larvae of the 2 species is the number of ventral setae [9 (4 circumanal pairs) in O. brodyi and 8 (3 circumanal pairs) in O. yumatensis]. The genetic divergence in 16S rDNA sequences between these two species ranges from 9.7 to 10.6%.
      
      

    • Presence of Borrelia in different populations of Ixodes pararicinus from northwestern Argentina (Completo, 2017)

      SARACHO BOTTERO, M.N. , SEBASTIAN, P.S. , CARVALHO, L. , CLAPS, L.G. , MASTROPAOLO, M. , MANGOLD, A.J. , VENZAL, J.M. , NAVA, S.
      Ticks and Tick-borne Diseases, v.: 8 p.:488 - 493, 2017
      Palabras clave: Ticks Argentina Ixodes Borrelia
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: 10.1016/j.ttbdis.2017.02.008
      This work was performed to evaluate the presence of Borrelia in different populations of Ixodes pararicinus from northwestern Argentina (Jujuy, Salta and Tucumán provinces). Questing adults and nymphs of I. pararicinus were collected from vegetation, and I. pararicinus nymphs were also collected on birds. Eighty-two ticks were tested for Borrelia presence by PCR targeting the gene flagellin and the rrfA-rrlB intergenic spacer region. Pools of ticks positive to Borrelia were formed by two nymphs collected on Turdus rufiventris in Tucumán, one nymph collected on Syndactyla rufosuperciliata in Jujuy, one nymph collected on Turdus nigriceps in Tucumán, three nymphs collected on T. nigriceps in Tucumán, and two females collected from vegetation in Salta. Two haplotypes of Borrelia sp. belonging to the Borrelia burgdorferi sensu lato complex were found. One of them is closely related to the haplotypes of Borrelia genospecies previously reported in I. aragaoi from Uruguay (haplotypes D and E) and in I. pararicinus from Jujuy Province in Argentina. The second haplotype (detected in the sample of Salta) is closely related to the haplotypes A, B and C associated with I. aragaoi from Uruguay. All these results suggest that the presence of B. burgdorferi s.l. genospecies in I. pararicinus ticks is widespread along the entire distribution of this tick species in northwestern Argentina. However, the Borrelia presence in I. pararicinus cannot be directly assumed as a phenomenon of medical relevance, because Ixodes ticks are not relevant as human parasites in South America, and none of the two Borrelia genospecies detected in this work is related to any of the Borrelia genospecies currently known to be pathogenic to humans.
      
      

    • Description of a new soft tick species (Acari: Argasidae: Ornithodoros) associated with stream-breeding frogs (Anura: Cycloramphidae: Cycloramphus) in Brazil. (Completo, 2017)

      MUÑOZ-LEAL, S. , TOLEDO, L.F. , VENZAL, J.M. , MARCILI, A. , MARTINS, T.F. , ACOSTA, I.C.L. , PINTER, A. , LABRUNA, M.B.
      Ticks and Tick-borne Diseases, v.: 8 p.:682 - 692, 2017
      Palabras clave: Ixodida New species Atlantic forest Amphibians Ectoparasites
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: 10.1016/j.ttbdis.2017.04.015
      In this study, we present a morphological description of immature and adult specimens of Ornithodoros saraivai n. sp., a tick associated with the frog Cycloramphus boraceiensis (Cycloramphidae) at São Sebastião island, located in the São Paulo state seaboard, Brazil. While larvae of O. saraivai are ecologically related to Ornithodoros faccinii, another soft tick associated with cycloramphids, the combination of 7 sternal pairs, 16 dorsal pairs, a pyriform dorsal plate and a partially toothed hypostome constitute unique characters of the O. saraivai larvae. One undetermined nymphal instar and adults of O. saraivai are similar to mature specimens of the Ornithodoros talaje species group; however, the O. saraivai specimens can be recognized by the presence of a robust bean-shaped spiracle with a large spiracular plate and more tan two long seta in palpal article I. Identical partial sequences of the mitochondrial 16S rDNA gene confirmed the identity for all collected stages and for two cohorts of laboratory-reared larvae of O. saraivai. A Bayesian and Maximum Parsimony inferred phylogenetic trees support the position of O. saraivai in a clade with O. faccinii, suggesting the existence of an Ornithodoros lineage that evolved in association with amphibians.
      
      

    • Coenurosis bovina en Uruguay (Completo, 2017)

      BURONI, F.; , ARMÚA, M.T. , NAN, F. , MATTO, C. , VENZAL, J.M. , RIVERO, R.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 54 206 , p.:19 - 24, 2017
      Palabras clave: Uruguay coenurosis bovina Coenurus cerebralis Bos taurus
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 03764362
      E-ISSN: 16884809
      Coenurosis es la enfermedad causada por Coenurus cerebralis, forma larvaria de Taenia multiceps. Afecta a rumiantes, equinos, suinos y humanos provocando sintomatología nerviosa. El presente trabajo tuvo como objetivo describir un foco de coenurosis en bovinos en Uruguay, diagnosticado mediante estudios anatomopatológicos y técnicas moleculares (PCR/secuenciación). Se realizó la necropsia de dos vaquillonas Hereford (Caso A y B) de 2-3 años que presentaban desmejoramiento progresivo, ataxia, torneo y desviación de la cabeza. Macroscópicamente, se observó la presencia de una vesícula quística en el hemisferio cerebral izquierdo del caso A, la cual se rompió espontáneamente al extraer el encéfalo del cráneo. En el caso B, se extrajo del hemisferio cerebral derecho una vesícula completa, de color transparente con escólices blanquecinos en su interior. Al examen histopatológico del sistema nervioso central se observó reacción inflamatoria en el tejido adyacente a la ubicación de los quistes constituida por macrófagos, eosinófilos, linfocitos y células gigantes. La vesícula del caso B fue diseccionada y se utilizó un trozo para la extracción de ADN. Mediante PCR se amplificó un fragmento del gen nad1. La secuencia obtenida fue comparada con las secuencias registradas en el GenBank utilizando la herramienta BLAST. El resultado reveló una homología de 99% con T. multiceps. Por lo tanto, este trabajo confirma que la coenurosis bovina se encuentra presente Uruguay.
      
      

    • Borrelia burgdorferi sensu lato in Ixodes longiscutatus ticks from Brazilian Pampa (Completo, 2017)

      DALL’AGNOL, B. , MICHEL, T. , WECK, B. , SOUZA, U.A. , WEBSTE, A. , LEAL, B.F , KLAFKE, G.M. , MARTINS, J.R. , OTT, R. , VENZAL, J.M. , FERREIRA, C.A.S. , RECK, J.
      Ticks and Tick-borne Diseases, v.: 8 p.:928 - 932, 2017
      Palabras clave: Brazil Borreliosis Bacteria Vector-borne infection Rio Grande do Sul Lyme disease
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: 10.1016/j.ttbdis.2017.08.003
      Borrelia burgdorferi sensu lato (s.l.) complex includes the agents of Lyme disease/borreliosis in North America, Europe, and Asia, such Borrelia burgdorferi sensu stricto, Borrelia afzelii, Borrelia garinii, Borrelia bavariensis, Borrelia spielmanii, Borrelia bissettiae, and Borrelia mayonii. In 2013 B. burgdorferi s.l. was reported for the first time in the Neotropical region, from Ixodes aragaoi ticks in Uruguayan Pampa. In addition, from 2011 to 2016, 17 suspected human cases of borreliosis-like syndrome were reported in Rio Grande do Sul (RS) state, Brazil, which contains only part of country in the Pampa biome. The goal of this work is to report the results of a state surveillance program conducted in order to investigate the presence of B. burgdorferi s.l. in its classic vector, Ixodes spp. ticks, from the Brazilian Pampa. For this, we searched for Ixodes spp. ticks in 307 rodents from 11 municipalities of RS state. We then tested the ticks for the presence of B. burgdorferi s.l. DNA using PCR analysis. Of 35 Ixodes spp. ticks tested, one larva and one nymph of Ixodes longiscutatus ticks tested positive for Borrelia sp. DNA. The phylogenetic analysis of the flaB fragment grouped our samples (referred as Borrelia sp. haplotype Pampa) into B. burgdorferi s.l. group in a particular branch with other South American haplotypes, and this group was close to Borrelia carolinensis, B. bissettiae, and Borrelia californiensis. This is the first evidence of B. burgdorferi s.l. circulation in ticks of the genus Ixodes in Brazil. These results highlight the need for the implementation of public health policies for the diagnosis and prevention of potential cases of human borreliosis in Brazil. Further studies are needed to fill the gaps in our knowledge of the distribution, pathogenicity, reservoirs, and vectors of these emerging South American B. burgdorferi s.l. haplotypes.
      
      

    • Descripción de un foco de rangeliosis canina en el litoral noroeste del Uruguay. (Completo, 2017)

      RIVERO, R. , MINOLI, P. , PARODI, P. , MATTO, C. , ARMúA-FERNáNDEZ MT , GIANNEECHINI, E. , CARVALHO, L. , VENZAL, J.M.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 54 208 , p.:15 - 22, 2017
      Palabras clave: Uruguay Rangelia vitalii Trombocitopenia rangeliosis canina anemia hemolítica
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 03764362
      E-ISSN: 16884809
      DOI: 10.29155/VET.23.208.3
      La rangeliosis canina es una enfermedad causada por el protozoario Rangelia vitalii y transmitida por garrapatas. Afecta caninos domésticos y silvestres, provocando un cuadro de anemia hemolítica y trombocitopenia. En Uruguay ha sido reportada en los Departamentos de Artigas, Salto y Treinta y Tres. El presente trabajo describe un foco de rangeliosis canina en el litoral oeste del Uruguay, diagnosticado mediante estudios epidemiológicos, clínicos, hematológicos, anatomopatológicos y técnicas moleculares (PCR/secuenciación). El estudio se basó en 9 casos de perros raza Griffon Fauve de Bretagne provenientes de un pre¬dio rural próximo a Quebracho, Departamento de Paysandú. De los animales ocho murieron y uno respondió al tratamiento. Los principales signos clínicos fueron apatía, ictericia, fiebre, diarrea con sangre y sangrado por narinas y punta de orejas. Hematológicamente se constató anemia, trombocitopenia y leucocitosis. Los hallazgos macroscópicos se caracterizaron por palidez, ictericia generalizada, esplenomegalia, linfoadenomegalia y hepatomegalia. Al examen histopatológico se destacó: nefritis intersticial con degeneración y necrosis tubular, miocarditis, necrosis hepática centrolobulillar. Congestión y edema pulmonar, linfoadenitis, y en bazo infiltrado inflamatorio difuso perivascular de tipo mononuclear. En diversos órganos se visualizaron zoítos de R. vitalii intracitoplasmáticas en las células endoteliales. En algunos animales así como en los caniles de los perros se constató presencia de Amblyomma aureolatum. De dos casos se extrajo ADN de sangre y mediante PCR se amplificó un fragmento del gen 18s ARN ribosomal de Piroplasmas. La secuencia obtenida fue comparada con secuencias registradas en el GenBank utilizando la herramienta BLAST. El resultado reveló una homología entre 99-100% con R. vitalii.
      
      

    • Ixodes spp. (Acari: Ixodidae) ticks in Rio Grande do Sul state, Brazil. (Completo, 2017)

      MICHEL, T. , SOUZA, U. , DALLAGNOL, B. , WEBSTE, A. , PETERS, F. , CHRISTOFF, A. , LUZA, A. , KASPER, N. , BECKER, M. , FIORENTIN, G. , KLAFKE, G. , VENZAL, J.M. , MARTINS, J. , JARDIM, M. , OTT, R. , RECK, J.
      Systematic and Applied Acarology, v.: 22 12 , p.:2057 - 2067, 2017
      Palabras clave: Pampa Atlantic Rainforest rodent Neotropic
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 13621971
      E-ISSN: 20566069
      DOI: 10.11158/saa.22.12
      Among the 251 described species of ticks from the genus Ixodes, only eight were previously reported in Brazil, Ixodes amarali, Ixodes aragaoi, Ixodes auritulus, Ixodes fuscipes, Ixodes loricatus, Ixodes luciae, Ixodes paranaensis and Ixodes schulzei. Of those species, I. loricatus is considered commonly found, whereas I. auritulus and I. aragaoi were registered just one time in Rio Grande do Sul (RS) state, Southern Brazil. This paper aims to update the Ixodes species occurring in RS through the investigation of the ixodofauna of small rodents. Data from 314 wild rodents were analyzed from nine municipalities of Pampa biome and five from the Atlantic Rainforest in RS. Rodents belonging to the following species were infested by Ixodes spp. ticks: Akodon azarae, Akodon montensis, Akodon paranaensis, Akodon reigi, Calomys laucha, Delomys dorsalis, Deltamys kempi, Holochilus brasiliensis, Oligoryzomys flavescens, Oligoryzomys nigripes, Oxymycterus nasutus, Scapteromys tumidus (Cricetidae) and Cavia aperea (Caviidae). The tick identification was performed based on morphological dichotomous keys, their updates and by molecular techniques. Considering the ticks from the rodents and those collected directly from the environment, 34 specimens of Ixodes spp. were collected. Our results improved to five the list of Ixodes species that occur in RS: I. loricatus, I. auritulus, I. aragaoi, I. fuscipes and I. longiscutatus. Moreover, the finding of I. longiscutatus increases to nine the number of Ixodes species in Brazil. The occurrence of two species of the Ixodes ricinus complex (I. aragaoi and I. fuscipes) highlights the potential impact of Ixodes spp. ticks on public health.
      
      

    • Molecular identification of Borrelia spirochetes in questing Ixodes ricinus from northwestern Spain (Completo, 2017)

      DíAZ, P. , ARNAL, J.L. , REMESAR, S. , PéREZ-CREO, A. , VENZAL, J.M. , VáZQUEZ-LóPEZ, M.E. , PRIETO, A. , FERNáNDEZ, G. , LóPEZ, C.F. , PANADERO, R. , BENITO, A. , DíEZ-BAñOS, P. , MORRONDO, P.
      Parasites & Vectors, v.: 10 615, p.:1 - 7, 2017
      Palabras clave: Ixodes ricinus Borrelia burgdorferi (s.l.) Borrelia miyamotoi NW Spain
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 17563305
      DOI: 10.1186/s13071-017-2574-x
      Background: Ixodes ricinus, the predominant tick species in Europe, can transmit the causative agents of important human diseases such as Lyme borreliosis (LB), caused by Borrelia spirochetes. In northern Spain, LB is considered endemic; recently, a significant increase of the annual incidence of LB was reported in the northwestern (NW) region. Methods: In order to provide information on the prevalence of Borrelia spp., pooled and individually free-living I. ricinus from NW Spain were molecularly analyzed. Positive samples were characterized at the fla and Glpq genes and the rrfA-rrlB intergenic spacer region to identify Borrelia species/genospecies. Results: Borrelia burgdorferi (sensu lato) (s.l.) individual prevalence and MIR were significantly higher in adult females (32.3 and 16%) than in nymphs (18.8 and 6.2%) and adult males (15.6 and 8.4%). Five Borrelia genospecies belonging to the B. burgdorferi (s.l.) group were identified: B. garinii was predominant, followed by B. valaisiana, B. lusitaniae, B. afzelii and B. burgdorferi (sensu stricto) (s.s.). One species belonging to the tick-borne relapsing fever group (B. miyamotoi) was also found, showing low individual prevalence (1%), positive pool (0.7%) and MIR (0.1%) values. To our knowledge, this is the first citation of B. miyamotoi in free-living ticks from Spain. Conclusions: The significant prevalences of B. burgdorferi (s.l.) genospecies detected in questing ticks from NW Spain are similar to those detected in northern and central European countries and higher to those previously found in Spain. These results together with the high incidence of LB in humans and the high seroprevalence of B. burgdorferi (s.l.) in roe deer shown in other studies reveal that the northwest area is one of the most risky regions for acquiring LB in Spain.
      
      

    • Rickettsiosis transmitidas por el grupo Amblyomma maculatum en Suramérica. (Completo, 2017)

      VENZAL, J.M.
      Revista Colombiana de Ciencias Pecuarias, v.: 30 p.:262 - 263, 2017
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      Escrito por invitación
      ISSN: 01200690
      E-ISSN: 22562958
      
      

    • Borrelia burgdorferi sensu lato in Ixodes cf. neuquenensis and Ixodes sigelos ticks from the Patagonian region of Argentina (Completo, 2016)

      SEBASTIAN, P.S. , SARACHO BOTTERO, M.N. , CARVALHO, L. , MACKENSTEDT, U. , LARESCHI, M. , VENZAL, J.M. , NAVA, S.
      Acta Tropica, v.: 162 p.:218 - 221, 2016
      Palabras clave: Argentina Ixodes sigelos Patagonia Borrelia burgdorferi sensu lato Ixodes cf. neuquenensis
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 0001706X
      DOI: 10.1016/j.actatropica.2016.06.030
      This study was conducted to detect Borrelia burgdorferi sensu lato infection in ixodid ticks from the Patagonia region in the south of Argentina. Therefore, ticks were collected on rodents in the provinces of Chubut, Río Negro and Santa Cruz. These ticks were identified as nymphs of Ixodes cf. neuquenensis and Ixodes sigelos. The B. burgdorferi s.l. infection was tested by a battery of PCR methods targeting the gene flagellin (fla) and the rrfA-rrlB intergenic spacer region (IGS). Three pools of I. sigelos nymphs from Chubut and Santa Cruz provinces as well as one pool of I. cf. neuquenensis nymphs from Río Negro province were tested positive in the fla-PCR. The samples of I. sigelos were also positive for the IGS-PCR. Phylogenetically,the haplotypes found in the positive ticks belong to the B. burgdorferi s.l. complex, and they were closely related to Borrelia chilensis, a genospecies isolated from Ixodes stilesi in Chile. The pathogenic relevance of the Borrelia genospecies detected in both I. neuquenensis and I. sigelos is unknown.
      
      

    • A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile. (Completo, 2016)

      OGRZEWALSKA, M. , NIERI-BASTOS, F.A. , MARCILI, A. , NAVA, S. , GONZÁLEZ-ACUÑA, D. , MUÑOZ-LEAL, S. , RUIZ-ARRONDOG, I. , VENZAL, J.M. , MANGOLD, A.J. , LABRUNA, M.B.
      Ticks and Tick-borne Diseases, v.: 7 p.:439 - 442, 2016
      Palabras clave: Chile Argentina Rickettsia Spotted fever group Amblyomma parvitarsum
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Medio de divulgación: Internet
      E-ISSN: 18779603
      DOI: 10.1016/j.ttbdis.2016.01.003
      The tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009–2013, and tested by PCR for rickettsial infection in the laboratory, and isolation of rickettsiae in Vero cell culture by the shell vial technique. Overall, 51 (62.2%) out of 82 A. parvitarsum adult ticks were infected by spotted fever group(SFG) rickettsiae, which generated DNA sequences 100% identical to each other, and when submitted toBLAST analysis, they were 99.3% identical to corresponding sequence of the ompA gene of Rickettsia sp. strain Atlantic rainforest. Rickettsiae were successfully isolated in Vero cell culture from two ticks, one from Argentina and one from Chile. DNA extracted from the third passage of the isolates of Argentina and Chile were processed by PCR, resulting in partial sequences for three rickettsial genes (gltA, ompB, ompA). These sequences were concatenated and aligned with rickettsial corresponding sequences avail-able in GenBank. Phylogenetic analysis revealed that the A. pavitarsum rickettsial agent grouped underhigh bootstrap support in a clade composed by the SFG pathogens R. sibirica, R. africae, R. parkeri, Rickettsia sp. strain Atlantic rainforest, and two unnamed SFG agents of unknown pathogenicty, Rickettsia sp.strain NOD, and Rickettsia sp. strain ApPR. The pathogenic role of this A. parvitarsum rickettsia cannot be discarded, since several species of tick-borne rickettsiae that were considered non pathogenic for decades are now associated with human infections.
      
      

    • Ixodes schulzei Aragão & Fonseca, 1951 in Argentina (Completo, 2016)

      LAMATTINA, D. , VENZAL, J.M. , GUGLIELMONE, A.A. , NAVA, S.
      Systematic and Applied Acarology, v.: 21 7 , p.:865 - 867, 2016
      Palabras clave: Ixodidae Argentina Sigmodontinae Rodentia Ixodes schulzei Cricetidae
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 13621971
      E-ISSN: 20566069
      DOI: 10.11158/saa.21.7.1
      Two females of Ixodes schulzei Aragão & Fonseca, 1951 were collected from Nectomys squamipes (Rodentia: Cricetidae: Sigmodontinae) at Iguazú National Park, Misiones Province, Argentina. This is the first record of this tick species in Argentina. All previous records of I. schulzei came from Brazil (some of them close to the border with Argentina) where larvae, nymphs and females of I. schulzei have been found parasitizing Sigmodontinae hosts. The current record of I. schulzei has been expected and its range may include Paraguay.
      
      

    • Redescription of Ornithodoros dyeri (Ixodida: Argasidae) based on morphologic and molecular data. (Completo, 2016)

      GUZMÁN-CORNEJO, C. , GARCíA-PRIETO, L. , NAVA, S. , VENZAL, J.M. , SáNCHEZ-MONTES, S. , MONTIEL-PARRA, G.
      Ticks and Tick-borne Diseases, v.: 7 p.:834 - 841, 2016
      Palabras clave: Acari bats Mexico Systematics Argasids
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 18779603
      DOI: 10.1016/j.ttbdis.2016.03.015
      Larvae, nymphs and adults of the cave dwelling tick Ornithodoros dyeri, collected in 3 Mexican states, werestudied using morphological and molecular methods. The adults and nymphs were characterized by an elongated body in proportion to the width and a dorsum bounded by two contiguous ridges and one third ridge (inner) that was incomplete on each side. The larvae of this species have 14 pairs of dorsal setae, a venter body with nine pairs of setae plus a posteromedian; a moderately large, dorsal plate and piriform, a hypostome arising from a relatively short, subtriangular median extension of the basis capituli, and a capsule of Haller´s organ with reticulations. Based on a maximum likelihood analysis of the sequences of a fragment of approximately 414 bp of the mitochondrial 16S rRNA gene, we showed that O. dyeri represents an independent lineage within neotropical species of the Argasidae. The bat species Mimon cozumelae and Peropteryx macrotis represents a new host record for this argasid.
      
      

    • A new species of Ornithodoros (Acari: Argasidae) from desert areas of northern Chile (Completo, 2016)

      MUÑOZ-LEAL, S. , VENZAL, J.M. , GONZÁLEZ-ACUÑA, D. , LOPES, M.G. , MARTINS, T.F. , FIGUEROA, C. , FERNáNDEZ, N. , LABRUNA, M.B.
      Ticks and Tick-borne Diseases, v.: 7 p.:901 - 910, 2016
      Palabras clave: Chile Argasidae Ornithodoros New species Liolaemus
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 18779603
      DOI: 10.1016/j.ttbdis.2016.04.008
      Ornithodoros atacamensis n. sp. is described from larvae collected on the lizard Liolaemus bisignatus and from free-living adults collected in desert areas from the Pan de Azúcar and Llanos de Challe National Parks, in Northern Chile. Additionally, unengorged larvae were obtained from fertilized females, which laid eggs in the laboratory. Morphological and mitochondrial 16S rDNA sequence analyses were performed in order to compare this new soft tick species with other congeneric Neotropical representatives. Larvae of O. atacamensis are morphologically closely related to Ornithodoros talaje sensu stricto, Ornithodoros puertoricensis, Ornithodoros rioplatensis, Ornithodoros guaporensis and Ornithodoros hasei, all belonging to the O. talaje species group. The larval diagnostic characters for this species are a combination of a large pyriform dorsal plate with a length of approximately 300 µm, 17 pairs of dorsal setae withfive central pairs, hypostome with apex pointed and dental formula 2/2 in most rows, 3/3 apically, and capsule of the Haller’s organ oval in shape without reticulations. Phylogenetic analyses inferred from the mitochondrial 16S rRNA gene and a Principal Component Analysis based on morphometric characters provide additional support to the description of O. atacamensis as an independent lineage within the genus clustering within the O. talaje species group.
      
      

    • First molecular characterization of Galba neotropica in Uruguay (Completo, 2016)

      ARMúA-FERNáNDEZ MT , CASTRO, O. , CORREA, O. , CARVALHO, L. , MANGOLD, A.J. , SANCHÍS, J. , VENZAL, J.M.
      FAVE, v.: 15 p.:9 - 13, 2016
      Palabras clave: Uruguay Galba neotropica molecular characterization
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 03253112
      DOI: favecv.v15i1/2.5978
      Until recently, it was believed that only two lymnaeid species (i.e. Galba viatrix and Pseudosuccinea columella) occurred in Uruguay. However, based on a molecular approach, an additional species Galba cubensis, was recently discovered. The aim of this study was to molecularly characterize different lymnaeid populations from the northern region of Uruguay. The lymnaeids collections were carried out in two farms of the departments of Paysandú and Tacuarembó. The collected lymnaeids were divided in two fractions, one fraction was used for conchological analyses and detection of trematode larval stages, while the other fraction was used for molecular studies. Three PCRs targeting the 16S, ITS-2 and COI DNA regions were performed and the amplicons obtained were direct sequenced. The sequences were used for homology search and construction of phylogenetic trees by the maximum-likelihood method. The sequencing results revealed that both isolates corresponded to Galba neotropica. The phylogenetic analyses placed our isolates among the G. neotropica monophyletic group, closely related to other isolates of this species found in several South American countries. To our knowledge, this is the first record of G. neotropica in Uruguay and the confirmation as competent intermediate host of Fasciola hepatica. Further studies are needed to define the distribution and the role of each lymnaeid species in the transmission of F. hepatica in Uruguay.
      
      

    • A new argasid tick species (Acari: Argasidae) associated with the rock cavy, Kerodon rupestris Wied-Neuwied (Rodentia: Caviidae), in a semiarid region of Brazil (Completo, 2016)

      LABRUNA, M.B. , NAVA, S. , MARCILI, A. , BARBIERI, A.R.M. , NUNES, P.H. , HORTA, M.C. , VENZAL, J.M.
      Parasites & Vectors, v.: 9 p.:511 - 511, 2016
      Palabras clave: Morphology Ornithodoros rietcorreai Kerodon rupestris Mitochondrial 16S rDNA ITS2
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 17563305
      DOI: 10.1186/s13071-016-1796-7
      Background: The rock cavy Kerodon rupestris (Wied-Neuwied, 1820) is a rodent species endemic to northeastern Brazil. Earlier studies have associated the argasid tick Ornithodoros talaje (Guérin-Méneville, 1849) with rocky cavy; however, a recent study proposed that O. talaje is not established in Brazil, where previous reports of this species were possibly misidentifications of closely related species, yet to be properly determined. Here, we describe a new species of Ornithodoros Koch, 1844 associated with rock cavies in northeastern Brazil. Methods: During 2012–2013, Ornithodoros ticks were collected from K. rupestris resting places in Paraíba State (PB) and Piauí State (PI), northeastern Brazil. These ticks were brought alive to the laboratory, and used to form two laboratory colonies (PB and PI ticks). Field-collected adults and laboratory-reared larvae were used for morphological description through light and scanning electron microscopy. DNA sequences of the mitochondrial 16S rRNA gene were generated from nymphal ticks and used to conduct phylogenetic analyses along with other Ornithodoros spp. sequences from GenBank. Reproductive compatibility of crosses between PB and PI adult ticks was evaluated, as well as analyses of hybrid ticks through larval morphology by a principal components analysis (PCA) and DNA sequences of the second internal transcribed spacer (ITS2) region from adult ticks. Results: Morphological analysis allowed recognizing these ticks as a new species, Ornithodoros rietcorreai n. sp. The larva of O. rietcorreai is distinct from those of other Ornithodoros spp. by the combination of the following carácter states: 14 pairs of dorsal setae, dorsal plate pyriform, hypostome with pointed apex and dental formula 3/3 anteriorly, 2/2 posteriorly, and anal valves with long and pointed leaf-shaped ends. There were a few larval morphological differences between PB and PI ticks, and their mitochondrial 16S rDNA sequences diverged by 3.3 %. On the other hand, cross-mating experiments showed that PB and PI ticks were reproductive compatible, indicating that they represent a single species. Analyses of ITS2 sequences and PCA corroborated this assumption. Conclusion: Ornithodoros rietcorreai is described as a new species associated with K. rupestris in Brazil, increasing the Brazilian tick fauna to 70 species.
      
      

    • Rickettsia lusitaniae associated with Ornithodoros yumatensis (Acari: Argasidae) from two caves in Yucatan, Mexico (Completo, 2016)

      SáNCHEZ-MONTES, S. , GUZMÁN-CORNEJO, C. , MARTíNEZ-NAJERA, Y. , BECKER, I. , VENZAL, J.M. , LABRUNA, M.B.
      Ticks and Tick-borne Diseases, v.: 7 p.:1097 - 1101, 2016
      Palabras clave: Mexico Rickettsia lusitaniae Ornithodoros yumatensis
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 1877959X
      DOI: 10.1016/j.ttbdis.2016.09.003
      The genus Rickettsia includes obligate intracellular bacteria transmitted by several hematophagous arthropods such as ticks, fleas and sucking lice. In particular hard ticks (Ixodidae) have been cited as the main vectors of pathogenic rickettsiae in Mexico. However, there have been only two records of a single Rickettsia species associated with Mexican soft ticks (Argasidae). In this study, we searched for rickettsial DNA in argasid ticks (13 adults and eight nymphs of Ornithodoros yumatensis) from two bat caves in the state of Yucatan, Mexico. Additionally one larva collected in a cave from Chiapas, Mexico, and associated with Desmodus rotundus was used to corroborate the tick taxonomic determination. Of these, nine ticks (43%) yielded expected PCR products for the rickettsial gltA gene. These PCR-positive ticks were tested with additional PCR protocols targeting the rickettsial genes gltA, ompA and ompB. DNA partial sequences from these genes showed 99–100% identities with Rickettsia lusitaniae, an agent isolated from O. erraticus in Portugal, and closely related to R. felis and R. hoogstraalii. Based on the results from this study, the inventory of rickettsiae distributed in Mexico increases from six to seven species.
      
      

    • Digeneos parásitos de Cyphocharax platanus (Günther, 1880) (Characiformes: Curimatidae) en el tramo inferior del río Uruguay en la zona de influencia de la represa de Salto Grande, Uruguay (Completo, 2016)

      VENZAL, J.M. , CASTRO, O. , SOSA, N. , FÉLIX, M.L. , LEITES, V. , MENONI, F. , VIGIL, J.D.
      FAVE, v.: 15 p.:20 - 30, 2016
      Palabras clave: Parásitos Digenea Río Uruguay Represa de Salto Grande Cyphocharax platanus
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 03253112
      DOI: 10.14409/favecv.v15i1/2.6130
      Entre agosto de 2012 y junio de 2013 estudios parasitológicos fueron llevados a cabo en 76 ejemplares de Cyphocharax platanus procedentes del tramo inferior del río Uruguay en la zona de influencia de la represa de Salto Grande. Tres taxones de digeneos fueron identificados: Saccocoelioides spp. (Haploporidae) y Zonocotyle bicaecata (Zonocotylidae) en intestino, y metacercarias de Sphincterodiplostomum musculosum (Diplostomidae) en el interior de los ojos. El 69,7% de los peces analizados resultaron parasitados por al menos un taxón de digeneo. Saccocoelioides spp. mostro los mayores valores de prevalencia, intensidad media y abundancia media (43,4%, 5,45, 2,37 respectivamente) de infección, en relación a Z. bicaecata (28,9%, 1,32 y 0,38 respectivamente) y S. musculosum (13,2%, 2,82 y 0,37 respectivamente). Saccocoelioides spp. y S. musculosum mostraron un patrón de distribución agregado, en tanto que Z. bicaecata presentó una distribución aleatoria en la población de hospedadores. No se hallaron diferencias significativas en los índices de infección de ninguno de los taxones de digeneos en relación al sexo de los hospedadores, y sólo Saccocoelioides spp. presentó una asociación significativa con el tamaño, siendo más frecuente en peces pequeños. Los dos parásitos intestinales (Saccocoelioides spp. y Z. bicaecata) no presentaron una asociación significativa entre sus índices de infección. Los registros de Saccocoelioides spp. y Z. bicaecata corresponden a los primeros para este hospedador en el río Uruguay, y el hallazgo de las metacercarias de S. musculosum constituye una nueva relación parásito-hospedador.
      
      

    • The taxonomic status of Rhipicephalus sanguineus (Latreille, 1806) (Completo, 2015)

      NAVA, S. , ESTRADA-PEÑA, A. , PETNEY, T. , BEATI, L. , LABRUNA, M.B. , SZABÓ, M.P.J. , VENZAL, J.M. , MASTROPAOLO, M. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Veterinary Parasitology, v.: 208 p.:2 - 8, 2015
      Palabras clave: DESCRIPTION Rhipicephalus sanguineus Type specimens Taxonomic status Neotype
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 03044017
      DOI: 10.1016/j.vetpar.2014.12.021
      The brown dog tick, Rhipicephalus sanguineus sensu stricto, is a species with considerable public health and economic importance. However, the taxonomic status of this species is far from resolved. After more than 110 years of scientific work on R. sanguineus s.s., the situationis that there is no type, no solid description, nor is there a consensus about the range of morphological variability within the species. Recent findings based on laboratory crossesnd molecular genetics strongly suggest that there are several entities grouped under thesame name. Here we review the history of the taxon, and we point out the caveats behindany further work on this tick. The current taxonomic status of R. sanguineus s.s. thus lacks an informative original description, and is based on the existence of several morphologicaldescriptions based on ticks originating from different populations, which show, in some cases, biological incompatibility and significant genetic divergence. We suggests that asa result it is not possible to assign the specific name R. sanguineus s.s. to any population. Further work is required based on the rules issued by the International Code of Zoological Nomenclature to clearly define the morphological range of the different populations.
      
      

    • Seroepidemiological survey of Rickettsia spp. in dogs from the endemic area of Rickettsia parkeri rickettsiosis in Uruguay. (Completo, 2015)

      LADO, P. , COSTA, F.B. , VERDES, J.M. , LABRUNA, M.B. , VENZAL, J.M.
      Acta Tropica, v.: 146 p.:7 - 10, 2015
      Palabras clave: Uruguay Amblyomma triste dogs Rickettsia parkeri Serosurvey
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 0001706X
      Rickettsia parkeri rickettsiosis is a vector-borne zoonosis that occurs in some countries of the American continent. Following the first description and determination of the pathogenicity to humans in 2004 in USA, this bacterium has been reported in several South American countries. Human cases have been diagnosed in both Uruguay and Argentina in the past years. This study consisted in a serosurvey of 1000 domestic dogs living in the endemic area of rickettsiosis in Uruguay, where Amblyomma triste is the tick vector. Sera were analyzed by Indirect Immunofluorescence Assay (IFA), against antigens of three different rickettsial species: R. rhipicephali, R. felis and R. parkeri. It was determined that 20.3% of the dogs had antibodies that reacted to at least one of the three species tested, taking as cut off ≥64 titers. Further more, 140 of the seropositive dogs (14%) had a titer at least 4 times higher to R. parkeri than those of any of the other species, thus, it was considered that the immune response was stimulated by that species in particular. This is the first serological survey in primary hosts for adults of A. triste in Uruguay, and therefore the first prevalence values are reported. Adult A. triste ticks collected from the environment as well as from dogs were analyzed by PCR in order to confirm the current circulation of the agent in the area. In this matter, two out of 28 ticks from dogs, and 3 out of 53 ticks from the environment were positive, and the corresponding sequence analysis revealed 100% similarity with R. parkeri strain maculatum.
      
      

    • Two new species of Ornithodoros (Ixodida; Argasidae) from the Southern Cone of South America (Completo, 2015)

      VENZAL, J.M. , GONZÁLEZ-ACUÑA, D. , MUÑOZ-LEAL, S. , MANGOLD, A.J. , NAVA, S.
      Experimental and Applied Acarology, v.: 66 p.:127 - 139, 2015
      Palabras clave: Chile Argentina Ornithodoros New species Systematics
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 01688162
      E-ISSN: 15729702
      DOI: 10.1007/s10493-015-9883-6
      Two new species of the genus Ornithodoros were described from larvae collected in Argentina and Chile. Ornithodoros xerophylus n. sp. was described from specimens collected on the small rodent Graomys centralis in Argentina. The diagnostic characters for this species are a combination of dorsal plate slightly oval with a length of approximately 250 lm, 16 pairs of dorsal setae, hypostome with apex rounded and dental formula 2/2 in most rows, 3/3 apically, and capsule of the Haller’s organ oval in shape without reticulations. Larvae of Ornithodoros lahillei n. sp. were collected on the reptiles Philodryas chamissonis and Callopistes maculatus in Chile. The diagnostic characters for O. lahillei are a combination of dorsal plate subtriangular with margins corrugated and posterior margin convex, dorsal surface with 14 pairs of setae, absence of postcoxal setae, and hypostome with apex pointed and dental formula 3/3 in anterior third and 2/2 in the middle and basal portion. Phylogenetic analysis of 16S rDNA sequences and a Principal Component Analysis based on morphometric characters provided additional support to the description of O. lahillei and O. xerophylus as two independent lineages within the genus Ornithodoros.
      
      

    • Molecular detection of Rangelia vitalii in domestic dogs from Uruguay (Completo, 2015)

      SOARES, J.F. , CARVALHO, L. , MAYA, L. , DUTRA, F. , VENZAL, J.M. , LABRUNA, M.B.
      Veterinary Parasitology, v.: 210 p.:98 - 101, 2015
      Palabras clave: Uruguay Rangelia vitalii Canine rangeliosis Dogs
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 03044017
      DOI: 10.1016/j.vetpar.2015.03.013
      The piroplasm Rangelia vitalii is the etiological agent of canine rangeliosis, a severe disease affecting domestic dogs in South America. Two domestic dogs from two different Departments (Salto and Treinta y Tres) of Uruguay presented with clinical signs such as apathy, anorexia, pale mucous membranes, jaundice, and hemorrhagic manifestations, suggestive of a canine vector-borne disease. Molecular analysis, based on PCR and DNA sequencing of portions of the 18S rRNA gene, revealed that both dogs were infected by R. vitalii. Two consensus sequences, one from Salto and one from Treinta y Tres, differed from each other by only 1 nucleotide (99.8% similarity) and were 99.8–100% identical to corresponding sequences of R. vitalii from Brazil and Argentina available in GenBank. Through phylogenetic analysis inferred by the 18S rRNA gene, the two Uruguayan sequences of R. vitalii were aligned with the corresponding sequences from 7 other R. vitalii sequences availablein GenBank (5 from Brazil and, 2 from Argentina) under high bootstrap support. The two dogs of the present study were negative for Ehrlichia canis according to the E. canis-specificreal-time PCR assay. Our findings not only confirm the occurrence of R. vitalii in Uruguay but also provide the southernmost record of this re-emerging agent. The only previous report of R. vitalii in Uruguay dated from 1976, a period when molecular analyses were not available. We provide the first molecular detection of R. vitalii in Uruguay. Currently, canine rangeliosis is confirmed to occur in Brazil, Argentina, and Uruguay.
      
      

    • Nothoaspis reddelli Keirans & Clifford, 1975 (Acari, Ixodida, Argasidae): distribution extensión (Completo, 2015)

      VENZAL, J.M. , HERNáNDEZ, L.V. , LABRUNA, M.B. , NAVA, S.
      Check List, v.: 11 4 1698, p.:1 - 2, 2015
      Palabras clave: Argasidae Ixodida Nicaragua
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 1809127X
      DOI: 10.15560/11.4.1698
      The first record of Nothoaspis reddelli (nymph) in Nicaragua is presented. The morphological characters used for the determination were dorsum with a false shield or nothoaspis, presence of an anteriorly projecting hood covering half of the capitulum, medial extension of palpal article I (flaps), presence of two large setae on internal margins of flaps, leg coxae with numerous long setae, and hypostome long and pointed with apical dentition 4/4. The 16S rDNA sequence of N. reddelli from Nicaragua was identical to the 16S sequence of N. reddelli from Mexico.
      
      

    • Riesgo de exposición a Fasciola hepatica en ganado vacuno en extensivo de Uruguay y Portugal determinado mediante ELISA y un antígeno recombinante (Completo, 2015)

      SANCHÍS, J. , HILLYER, G.V. , MADEIRA DE CARVALHO, L.M. , MACCHI, M.I. , GOMES, C. , MALDINI, G , STILWELL, G. , VENZAL, J.M. , PAZ-SILVA, A. , SÁNCHEZ-ANDRADE, R. , ARIAS, M.S.
      Archivos de medicina veterinaria, v.: 47 p.:201 - 208, 2015
      Palabras clave: cattle fasciolosis grazing risk assessment immunoassay
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      E-ISSN: 0301732X
      
      

    • Ornithodoros (Alectorobius) puertoricensis (IXODIDA: ARGASIDAE) PARASITIZING EXOTIC REPTILES PETS IN PANAMÁ. (Completo, 2015)

      BERMÚDEZ, S.E. , ROBERTO J. MIRANDA , CLEGHORN, J. , VENZAL, J.M.
      FAVE, v.: 14 p.:1 - 5, 2015
      Palabras clave: Ornithodoros puertoricensis exotic reptiles Panamá
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03253112
      DOI: 10.14409/favecv.v14i1/3.5095
      
      

    • New tick records from the state of Rondônia, western Amazon, Brazil. (Completo, 2014)

      MARTINS, T.F. , VENZAL, J.M. , TERASSINI, F.A. , COSTA, F.B. , MARCILI, A. , CAMARGO, L.M.A. , BARROS-BATTESTI, D.M. , LABRUNA, M.B.
      Experimental and Applied Acarology, v.: 62 p.:121 - 128, 2014
      Palabras clave: Amblyomma auricularium Amblyomma dubitatum Amblyomma geayi Ornithodoros kohlsi Rondônia Brazil
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      E-ISSN: 15729702
      DOI: 10.1007/s10493-013-9724-4
      From 2005 to 2012, ticks were collected from different hosts at different localities of the state of Rondônia. The following 16 ixodid tick species were identified: Ixodes fuscipes, Amblyomma auricularium, Amblyomma coelebs, Amblyomma dubitatum, Amblyomma geayi, Amblyomma humerale, Amblyomma latepunctatum, Amblyomma longirostre, Amblyomma naponense, Amblyomma nodosum, Amblyomma oblongoguttatum, Amblyomma ovale, Amblyomma romitii, Amblyomma rotundatum, Amblyomma scalpturatum, and Amblyomma varium. From these, A. auricularium, A. dubitatum, and A. geayi are reported for the first time in the state of Rondônia. We provide the following tick-host associations that have not been reported anywhere: A. longirostre on Pteroglossus bitorquatus, A. rotundatum on Hydrodynastes gigas, and A. latepunctatum and A. scalpturatum on Hydrochoerus hydrochaeris. An adult male specimen of A. rotundatum is reported on Boa constrictor, comprising only the fourth male specimen to be recorded for this obligate parthenogenetic tick species. We also report the presence of the argasid species Ornithodoros kohlsi for the first in Brazil, based on larval specimens collected on bats Molossops (Neoplatymops) mattogrossensis in Monte Negro, Rondônia. The present study increases the Brazilian tick fauna to 65 species, from which 34 species (52.3%) are now registered to Rondônia. Such high diversity of ticks in a relatively small state, associated with increasing environmental alteration due to deforestation and human occupation, makes Rondônia a potential source of tick-borne diseases.
      
      

    • New Records and Human Parasitism by Ornithodoros mimon (Acari: Argasidae) in Brazil (Completo, 2014)

      LABRUNA, M.B. , MARCILI, A. , OGRZEWALSKA, M. , BARROS-BATTESTI, D.M. , DANTAS-TORRES, F. , FERNANDES, A.A. , LEITE, R.C. , VENZAL, J.M.
      Journal of Medical Entomology, v.: 51 1 , p.:283 - 287, 2014
      Palabras clave: Ornithodoros mimon bat Brazil human infestation opossum
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Medio de divulgación: Papel
      ISSN: 00222585
      E-ISSN: 19382928
      DOI: 10.1603/ME13062
      The bat tick Ornithodoros mimon Kohls, Clifford & Jones is currently known by only few reports in Bolivia, Uruguay, Argentina, and the state of Sao Paulo in southeastern Brazil. Here, we expand the distribution of O. mimon in Brazil to the states of Minas Gerais (southeastern region), Goiás (central-western), Pernambuco, and Rio Grande do Norte (northeastern). Ticks were collected on human dwellings, where there had been repeated complains of tick bites on persons during the night. Tick bites were generally followed by intense inßammatory reactions that lasted for several weeks at the bite site. Bats and opossums were reported to inhabit the attic of the infested houses. In addition, a free-ranging opossum (Didelphis albiventris Lund) trapped in Rio Grande do Norte was found infested by argasid larvae. Based on morphological and/or molecular analysis, all ticks were identiÞed as O. mimon. From one of the sites (Tiradentes, state of Minas Gerais), 20 field-collected nymphs were tested by a battery of polymerase chain reaction protocols targeting tick-borne microorganisms of the genera Babesia, Hepatozoon, Rickettsia, Borrelia, Anaplasma, Ehrlichia, and Coxiella; no tick specimen was found infected by any of these microorganism genera. The current study expands northwards the distribution of O. mimon, which has been shown to be very harmful to humans because of the intense inßammatory response that usually occurs after tick bites.
      
      

    • Guías Latinoamericanas de la RIICER para el diagnóstico de las rickettsiosis transmitidas por garrapatas. (Completo, 2014)

      OTEO, J.A. , NAVA, S. , DE SOUZA, R. , MATTAR, S. , VENZAL, J.M. , ABARCA, K. , LABRUNA, M.B. , ZAVALA-CASTRO, J.
      Revista Chilena de Infectología, v.: 31 1 , p.:54 - 65, 2014
      Palabras clave: Garrapatas Rickettsiosis guías Latinoamérica inmunofluorescencia indirecta cultivo celular
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Medio de divulgación: Papel
      ISSN: 07161018
      E-ISSN: 07176341
      Tick-borne rickettsioses are worldwide infectious diseases that are considered emerging and re-emerging. Until recently the only tick-borne rickettsiosis present in Latin America was Rickettsia rickettsii infection, but to date, with the incorporation of new tools as PCR and sequencing and the quick cellular close tube cultures (Shell-vial), new species has been involved as human pathogens. In these guidelines, we offer an update of the microbiological assays for diagnosing rickettsioses. Besides we have included a section in which the most important hard ticks involved in human rickettsioses in Latinoamerica are detailed.
      
      

    • El género Amblyomma (Acari: Ixodidae) en Uruguay: especies, distribución, hospedadores, importancia sanitaria y claves para la determinación de adultos y ninfas. (Completo, 2014)

      MARTINS, T.F. , LADO, P. , LABRUNA, M.B. , VENZAL, J.M.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 50 193 , p.:26 - 41, 2014
      Palabras clave: Uruguay AMBLYOMMA hospedadores distribución claves dicotómicas
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03764362
      E-ISSN: 16884809
      En el presente trabajo se presentan las claves para la identificación de adultos y ninfas de las siete especies del género Amblyomma que son consideradas como residentes en Uruguay: Amblyomma aureolatum (Pallas, 1772), Amblyomma auricularium (Conil, 1878), Amblyomma dubitatum Neumann, 1899, Amblyomma longirostre (Koch, 1844), Amblyomma pseudoconcolor Aragão, 1908, Amblyomma tigrinum Koch, 1844 y Amblyomma triste Koch, 1844. Así mismo se realiza una actualización sobre la distribución, hospedadores e importancia médico-veterinaria de cada una de la especies.
      
      

    • Amblyomma hadanii n. sp. (Acari: Ixodidae), a tick from northwestern Argentina previously confused with Amblyomma coelebs Neumann, 1899. (Completo, 2014)

      NAVA, S. , MASTROPAOLO, M. , MANGOLD, A.J. , MARTINS, T.F. , VENZAL, J.M. , GUGLIELMONE, A.A.
      Systematic Parasitology, v.: 88 p.:261 - 272, 2014
      Palabras clave: AMBLYOMMA Ticks Argentina New species
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Papel
      ISSN: 01655752
      E-ISSN: 15735192
      DOI: 10.1007/s11230-014-9500-9
      All stages of Amblyomma hadanii n. sp. (Acari: Ixodidae) are described from northwestern Argentina. The diagnostic characters for males are a combination of the pattern of scutal ornamentation, basis capituli dorsally rectangular with cornua, coxa I with two subequal spurs (the internal wider, the external longer), coxae II-III with a single spur, coxa IV with a single spur not reaching level of anus, ventral plates irregular in shape (larger and sometimes with a small incision on festoons 4, 5 and 6) and hypostome spatulate with dental formula 3/3 in 7–8 rows. The diagnostic characters for the females are a combination of scutal ornamentation, postero-lateral margins of scutum slightly convex, coxa I with two subequal spurs (the internal wider, the external longer), basis capituli dorsally rectangular, porose areas rounded, genital aperture U-shaped, and hypostome spatulate with dental formula 3/3 in 7–8 rows. Diagnosis of nymphs can be performed by a combination of basis capituli rectangular, scutum with large punctations in the lateral fields and small punctations in the central field, and cervical groove short and ending as a small shallow depression at the eye level. Larvae are diagnosed by the shape of basis capituli, scutum with with posterior margin slightly convex, and legs with coxa I with 2 triangular spur (the external longer than the internal), and with coxae II and III each with 1 triangular spur. The hosts recorded for this new tick species are Tapirus terrestris (Linnaeus), horse, cattle, dog and humans. Analyses of a 410 bp fragment of the mitochondrial 16S rRNA gene and the complete sequence of the nuclear 18S rRNA gene supported the description of A. hadanii as a new species.
      
      

    • Borrelia infection in Ixodes pararicinus ticks (Acari: Ixodidae) from northwestern Argentina. (Completo, 2014)

      NAVA, S. , BARBIERI, A.M. , MAYA, L. , COLINA, R. , MANGOLD, A.J. , LABRUNA, M.B. , VENZAL, J.M.
      Acta Tropica, 139 , p.:1 - 4, 2014
      Palabras clave: Argentina Ixodes pararicinus Borrelia burgdorferi sensu lato
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Medio de divulgación: Papel
      ISSN: 0001706X
      The aim of this work was to describe for the first time the presence of Borrelia burgdorferi sensu lato infecting ticks in Argentina. Unfed specimens of Ixodes pararicinus collected from vegetation in Jujuy Province were tested for Borrelia infection by PCR targeting the gene flagellin (fla), the rrfA-rrlB intergenic spacer region (IGS) and the 16S rDNA (rrs) gene. One male and one female of I. pararicinus collected in Jujuy were found to be positive to Borrelia infection with the three molecular markers tested. Phylogenetically, the Borrelia found in I. pararicinus from Jujuy belongs to the B. burgdorferi s.l complex, and it was similar to one of the genospecies detected in I. aragaoi from Uruguay. Also, this genospecies is closely related to two genospecies known from USA, Borrelia americana and the Borrelia sp. genospecies 1. The epidemiological risk that implies the infection with Borrelia in I. paracinus ticks from Argentina appears to be low because the genospecies detected is not suspected of having clinical relevance and there are no records of Ixodes ticks biting humans in the southern cone of South America. Further studies are needed to assess accurately if there is risk of borreliosis transmitted by ticks in South America.
      
      

    • First molecular detection of Rickettsia parkeri in Amblyomma tigrinum and Amblyomma dubitatum ticks from Uruguay (Completo, 2014)

      LADO, P. , CASTRO, O. , LABRUNA, M.B. , VENZAL, J.M.
      Ticks and Tick-borne Diseases, v.: 5 p.:660 - 662, 2014
      Palabras clave: Uruguay Amblyomma tigrinum Ticks Rickettsia parkeri Amblyomma dubitatum
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Medio de divulgación: Papel
      ISSN: 1877959X
      Rickettsia parkei is the etiological agent of spotted fever in Uruguay, where is transmitted to humans by the tick Amblyomma triste. In the present study, ticks were collected from capybaras (Hydrochoerus hydrochaeris) and domestic dogs during 2011-2012 in different parts of Uruguay. Three out of 11 (27.3%) Amblyomma dubitatum ticks collected from capybaras, and 4 out of 6 (66.7 %) Amblyomma tigrinum ticks collected from dogs were shown by molecular analyses to be infected by Rickettsia parkeri strain Maculatum 20. Until the present work, A. triste was the only tick species that was found infected by R. parkeri in Uruguay. This is the first report of R. parkeri infecting these two tick species in Uruguay, expanding the current distribution of this rickettsial pathogen in the country.
      
      

    • Registro de la larva de Leiperia gracilis (Pentastomida, Sebekidae) en un dorado, Salminus brasiliensis (Characidae), en el río Uruguay. (Completo, 2014)

      CASTRO, O. , VENZAL, J.M. , FÉLIX, M.L.
      Boletín de la Sociedad Zoológica del Uruguay, v.: 23 1 , p.:30 - 35, 2014
      Palabras clave: Uruguay Salminus brasiliensis Leiperia gracilis Pentastomida
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 02554402
      E-ISSN: 23936940
      Se comunica el hallazgo de una larva de Leiperia gracilis (Pentastomida) en el hígado de un dorado, Salminus brasiliensis, colectado en el río Uruguay, en el puerto de la ciudad de Salto. Es el primer registro de esta especie para nuestro país, discutiéndose brevemente los registros previos de pentastómidos para Uruguay.
      
      

    • Ticks (Acari: Ixodidae) on swifts (Apodiformes: Apodidae) in Minas Gerais, southeastern Brazil (Completo, 2014)

      TOLESANO-PASCOLI , G. , GARCIA, F.I. , GOMES, C.R.G. , DINIZ, K.C. , ONOFRIO, V.C. , VENZAL, J.M. , SZABÓ, M.P.J.
      Experimental and Applied Acarology, v.: 64 p.:259 - 263, 2014
      Palabras clave: Ixodidae Argasidae Ticks Swifts Waterfalls Southeastern Brazil
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 01688162
      E-ISSN: 15729702
      DOI: 10.1007/s10493-014-9797-8
      Brazil harbors five species of Cypseloidinae swifts. Those from Streptoprocne and Cypseloides genera have a very distinct ecology. They shelter at night and build nests in moist cliffs by waterfalls. Information about tick infestation of these birds is virtually non-existent and restricted to the description of a new species, Ixodes paranaensis, in Streptoprocne biscutata in Parana´ State and another record of this species in Streptoprocne zonaris in Minas Gerais State. We herein report tick infestation of swifts at eight waterfalls in the Cerrado biome of Minas Gerais State, southeastern Brazil. Swifts were captured during six campaigns from November 2008 to April 2013. Overall, 584 swifts were captured (527 C. senex, four C. fumigatus and 53 S. zonaris). Four birds were tick infested (prevalence of 0.7 %). Three individuals of C. senex hosted one tick each; a nymph of I. paranaensis, a female of I. paranaensis and a nymph of Amblyomma cajennense. One S. zonaris hosted an I. paranaensis nymph and an Ornithodoros sp. larva (Argasidae).
      
      

    • Validation of the taxon Ixodes aragaoi Fonseca (Acari: Ixodidae) based on morphological and molecular data (Completo, 2014)

      ONOFRIO, V.C. , DIEGO G. RAMIREZ , DALTON N. S. GIOVANNI , MARCILI, A. , MANGOLD, A.J. , VENZAL, J.M. , MENDONçA, R.Z. , LABRUNA, M.B. , BARROS-BATTESTI, D.M.
      Zootaxa, v.: 3860 4 , p.:361 - 370, 2014
      Palabras clave: Uruguay Brazil Ixodes aragaoi taxon validity
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Internet
      ISSN: 11755326
      E-ISSN: 11755334
      DOI: 10.11646/zootaxa.3860.4.4
      The species Ixodes aragaoi Fonseca was described as Ixodes ricinus aragaoi, and later placed in synonymy with Ixodes affinis. However, this synonymy was rejected and the subspecies was elevated to species, and named as I. aragaoi. Some researchers did not consider the validity of I. aragaoi and maintained the synonymy proposed until 1998 when I. aragaoi was revalidated, and it was suggested that Ixodes pararicinus could be a synonym. The aim of this study was to confirm the taxonomic validity of I. aragaoi by means of redescription of adults and molecular analysis. Morphological studies were performed by optical and scanning electron microscopy; types of I. aragaoi were compared with those of I. pararicinus from Argentina, and also with material of I. pararicinus from Uruguay and I. affinis from the United States. Mitochondrial 16S rDNA sequences were obtained for determining phylogenetic relationships based on maximum parsimony. Morphological and molecular differences between I. aragaoi, I. pararicinus from Argentina, and I. affinis confirm the validity of the first each of these species. The morphological similarities of I. pararicinus from Uruguay with I. aragaoi, and the small distance of nucleotide sequences between them, confirm that the Uruguayan ticks are in fact I. aragaoi and expand the geographical distribution of this species. Based on the specimens of Ixodes examined in the present study, from the same locality of the types of I. ricinus rochensis in Uruguay, we agree with the synonymy of this subspecies with I. aragaoi as previously reported. Finally, our analyses indicate that both I. aragaoi and Ixodes fuscipes, another South American tick species, belong to the I. ricinus complex, currently composed of 19 species.
      
      

    • The presence of Argas keiransi Estrada-Peña, Venzal & González-Acuña, 2003 (Acari: Argasidae) in Argentina (Completo, 2014)

      VENZAL, J.M. , FLORES, S.F. , SOLARO, C. , SANTILLáN, M.A. , MANGOLD, A.J. , NAVA, S.
      Systematic and Applied Acarology, v.: 19 4 , p.:399 - 403, 2014
      Palabras clave: Argentina 16S rDNA sequences Argas keiransi Milvago chimango
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Papel
      ISSN: 13621971
      E-ISSN: 20566069
      DOI: 10.11158/saa.19.4.3
      Forty-two larvae of Argas keiransi (Acari: Argasidae) were collected from the Chimango Caracara, Milvago chimango (Falconiformes: Falconidae), at three localities in La Pampa Province, Argentina. Ticks were determined by a combination of palpal segment IV as long as or longer than the other palpal segments, absence of a trumpet-shaped sensillum extending from the capsule of Haller ́s organ into the lumen of the tarsus, length of dorsal plate between 180 and 240 μm, length of hypostome between 180 and 205 μm, dental formula 2/2 in the basal third, 2/2 or 3/3 apically, and length of posterolateral setae between 45 and 50 μm. Morphological determination was confirmed by analysis of sequences of the mitochondrial 16S rRNA gene. The results of this work constitute the first Argentinean record of A. keiransi, which has previously been reported only from Chile.
      
      

    • ‘Candidatus Rickettsia asemboensis’ and Wolbachia spp. in Ctenocephalides felis and Pulex irritans fleas removed from dogs in Ecuador. (Completo, 2014)

      OTEO, J.A. , PORTILLO, A. , PORTERO, F. , ZAVALA-CASTRO, J. , VENZAL, J.M. , LABRUNA, M.B.
      Parasites & Vectors, v.: 7 455 , p.:1 - 5, 2014
      Palabras clave: Ctenocephalides felis fleas Ecuador Pulex irritans ‘Candidatus Rickettsia asemboensis’ Wolbachia spp.
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Medio de divulgación: Internet
      E-ISSN: 17563305
      DOI: 10.1186/s13071-014-0455-0
      Background: Flea-borne infections are distributed worldwide. Up to date there are no reports about microorganisms associated to fleas in Ecuador. Methods: Seventy-one Pulex irritans and 8 Ctenocephalides felis fleas were removed from dogs in two Ecuadorian areas (Pastaza and Chimborazo Provinces) in December 2012. DNA extracts were tested by polymerase chain reaction (PCR) assays targeting universal 16S rRNA, as well as screened for the presence of Rickettsia spp. (gltA, htrA, ompB, sca4 and ompA genes) and Bartonella spp. (rpoB, gltA and ITS genes). Results: Our results showed the presence of ‘Candidatus Rickettsia asemboensis’ (highly similar to R. felis) in C. felis and Wolbachia spp. endosimbionts in P. irritans collected from animals in Ecuador. No fleas were found to be positive for any Bartonella species or Yersinia pestis. Conclusions: Clinicians should be aware of the potential risk of this new Candidatus Rickettsia sp. and keep in mind other flea-borne infections since these flea species frequently bite humans.
      
      

    • Infection by Paramphistomidae trematodes in cattle from two agricultural regions in NW Uruguay and NW Spain (Completo, 2013)

      SANCHÍS, J. , SÁNCHEZ-ANDRADE, R. , MACCHI, M.I. , PIÑEIRO, P. , SUÁREZ, J.L , CAZAPAL-MONTEIRO, C. , MALDINI, G , VENZAL, J.M. , PAZ-SILVA, A. , ARIAS, M.S.
      Veterinary Parasitology, v.: 191 p.:165 - 171, 2013
      Palabras clave: Uruguay ELISA Paramphistomidae Bovine Spain Risk factors
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03044017
      The analysis of infection by Paramphistomidae trematodes was conducted in two agricultural regions with different knowledge on this parasitosis. Faecal and blood samples were collected from 374 cattle in Salto (NW Uruguay) where there is a lack of information about paramphistomosis. A total of 429 cattle from Galicia (NW Spain), an area with previous records of infection by gastric flukes, were sampled. Diagnostics of trematodosis was developed by using a copromicroscopic probe and an ELISA with excretory/secretory antigens collected from adult Calicophoron daubneyi (Paramphistomidae) specimens. Results were evaluated according intrinsic and extrinsic factors. In the Uruguay, the percentage of cattle passing Paramphistomidae-eggs by faeces was 7% (95% Confidence Interval 5, 10). A significantly higher prevalence of paramphistomosis in the Hereford × Angus cattle (OR = 3.5) was recorded, as observed for the oldest ruminants (>3.5 years). An overall seroprevalence of 29% (25, 34) was obtained by ELISA, with the highest values in the Friesians (OR = 3), the youngest bovines (<2.5 years) and dairy cattle (Friesians). Twenty-six percent (22, 30) of the cattle from Spain passed eggs by faeces, and cattle aged 2.5–7 years reached significant highest prevalences. By means of the ELISA, a percentage of 55 cattle (50, 59) had antibodies against the gastric fluke, and the highest seroprevalence was observed among the bovines under 6 years. It is concluded that paramphistomosis is on the increase in cattle from NW Spain, partly due to the absence of an effective treatment against the trematode. There is a need for reducing the risk of infection by Paramhistomidae spp. in cattle from Uruguay, especially by improving their management to avoid exposure to the gastric trematode. Further studies are in progress for identifying the species of Paramphistomidae affecting ruminants in Uruguay.
      
      

    • New records of Amblyomma multipunctum and Amblyomma naponense from Ecuador, with Description of A. multipunctum Nymph (Completo, 2013)

      LABRUNA, M.B. , MARTINS, T.F. , NUNES, P.H. , BORGES, F.C. , VENZAL, J.M. , PORTERO, F.
      Journal of Parasitology, v.: 99 6 , p.:973 - 977, 2013
      Palabras clave: Amblyomma multipunctum Amblyomma naponense Ecuador
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Papel
      ISSN: 00223395
      E-ISSN: 19372345
      DOI: 10.1645/13-254.1
      We provide new collection data for the ticks Amblyomma multipunctum and Amblyomma naponense in Ecuador. In addition, we describe the nymph of A. multipunctum for the first time. During December 2012, ticks were collected by dragging in forest trails of 1 locality at Puyo, Pastaza Province (elevation 979 m), and another locality at Papallacta, Napo Province (3,474 m). A total of 10 adults of A. naponense were collected at Puyo, whereas 27 adults and 3 nymphs of A. multipunctum were collected at Papallacta. Taxonomic identification of the A. multipunctum nymph was initially performed by molecular analysis based on sequences of a fragment of the mitochondrial 16S rDNA gene of adult and nymphal ticks. The sequence of an Amblyomma nymph was identical to the sequences generated from the A. multipunctum adults. The 3 collected nymphs (including the 1 used for molecular analysis) had the same morphotype, and were used for the first morphological description of the nymphal stage of A. multipunctum. Sequences generated from the A. naponense specimens showed by BLAST analysis to be closest (97% identity) to a corresponding sequence of A. naponense from Brazil, whereas the A. multipunctum sequences showed to be closest (90-91% identity) to a number of Neotropical Amblyomma species. Herein, we provide just the second record of A. naponense in Ecuador, more than 100 yrs after this tick was reported in that country. Adults and nymphs of A. multipunctum were collected in a highland, humid montane forest area, in agreement with the only 2 previous reports of A. multipunctum in Ecuador and Colombia. Finally, no genetic differences were found among A. multipunctum ticks that presented significant morphological differences, indicating that there is intraspecific polymorphism in the adult stage of this species.
      
      

    • Borrelia burgdorferi sensu lato infecting ticks of the Ixodes ricinus complex in Uruguay: first report for the southern hemisphere. (Completo, 2013)

      BARBIERI, A.M. , VENZAL, J.M. , MARCILI, A. , ALMEIDA, A.P. , GONZÁLEZ, E.M. , LABRUNA, M.B.
      Vector-Borne and Zoonotic Diseases, v.: 13 3 , p.:147 - 153, 2013
      Palabras clave: Uruguay SOUTH AMERICA Ixodes pararicinus Borrelia burgdorferi
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Salud Pública y Medioambiental / Parasitología
      Medio de divulgación: Papel
      ISSN: 15303667
      E-ISSN: 15577759
      DOI: 10.1089/vbz.2012.1102
      The Borrelia burgdorferi sensu lato (s.l.) group comprises genetically related spirochaetes, mostly associated with tick species belonging to the Ixodes ricinus complex in the northern hemisphere. The present study evaluated borrelial infection in the tick Ixodes pararicinus, which is the only representative species of the I. ricinus complex in Uruguay. A total of 137 I. pararicinus ticks were collected from deer, cattle or vegetation in two Uruguayan Departments. Part of these ticks was tested directly by PCR targeting the borrelial gene flagellin (fla), whereas another part of the ticks was inoculated into Barbour-Stoenner-Kelly (BSK)-H medium in an attempt to isolate Borrelia. Overall, Borrelia infection was detected in 9 males and one nymphal tick pool. These ticks were found to be infected by unique fla haplotypes, which were shown through phylogenetic analysis to represent possibly two new B. burgdorferi s.l. genospecies, one associated with B. bissettii, the other phylogenetically closest to B. americana. These results were reinforced by PCR and DNA sequencing analyses of portions of two additional borrelial genes, rrfA-rrlB intergenic spacer region (IGS) and 16S rDNA (rrs). Weekly examinations of BSK cultures by dark field microscopy failed to demonstrate live Borrelia through a 100-day incubation period. However, Borrelia DNA was detected by fla-PCR in culture media from two vials up to 90 days after inoculation. To the best of our knowledge, this is the first report of B. burgdorferi s.l. infecting ticks in South America.
      
      

    • A new species of Ornithodoros (Acari: Argasidae), parasite of Microlophus spp. (Reptilia: Tropiduridae) from northern Chile. (Completo, 2013)

      VENZAL, J.M. , NAVA, S. , GONZÁLEZ-ACUÑA, D. , MANGOLD, A.J. , MUÑOZ-LEAL, S. , LADO, P. , GUGLIELMONE, A.A.
      Ticks and Tick-borne Diseases, v.: 4 p.:128 - 132, 2013
      Palabras clave: Chile Argasidae Ornithodoros microlophi sp. n. Microlophus spp. Tropiduridae
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Papel
      ISSN: 1877959X
      A new species, Ornithodoros microlophi (Acari: Argasidae), (Acari: Argasidae) belonging to the subgenus Alectorobius is described from larvae collected on the lizards Microlophus atacamensis (Donoso-Barros, 1966) and Microlophus quadrivittatus (Tschudi, 1845) (Squamata: Tropiduridae) in continental and insular localities from northern Chile. Larvae of O. microlophi can be distinguished from other Neotropical species of the genus Ornithodoros by a combination of the following characters, namely 10 pairs of ventral setae, venter with six pairs of sternal setae, dorsal plate pyriform, 19 to 21 pairs of dorsal setae (typically 20), 13 pairs are dorsolateral and 7 pairs are central, and hypostome with dental formula 4/4 in medial portion and apex pointed. Phylogenetic analysis of 16S rDNA sequences suggests that O. microlophi represent an independent lineage within Neotropical species of Argasidae.
      
      

    • Ornithodoros peropteryx (Acari: Argasidae) in Bolivia: an argasid tick with a single nymphal stage. (Completo, 2013)

      VENZAL, J.M. , NAVA, S. , TERASSINI, F.A. , OGRZEWALSKA, M. , CAMARGO, L.M.A. , LABRUNA, M.B.
      Experimental and Applied Acarology, v.: 61 p.:231 - 241, 2013
      Palabras clave: Argasidae Ornithodoros Morphology Bolivia
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Papel
      E-ISSN: 15729702
      DOI: 10.1007/s10493-013-9689-3
      • Marcelo B. Labruna
      By the end of the 1960s, the argasid tick Ornithodoros peropteryx was described from larval specimens collected from the bat Peropteryx macrotis in Colombia. Since its original description, no additional record of O. peropteryx has been reported, and its post-larval stages have remained unknown. During July 2010, 18 larvae were collected from 9 bats (Centronycteris maximiliani), resulting in a mean infestation of 2.0 ± 2.2 ticks per bat (range 1–8). These bats were captured in a farm in northeastern Bolivia close to Guaporé River in the border with Brazil. Morphological examinations of the larvae revealed them to represent the species O. peropteryx. One engorged larva that was kept alive in the laboratory moulted to a nymph after 9 days. Fourteen days after the larval moulting, the nymph moulted to an adult female without taking any blood meal during the nymphal period. This adult female was used for a morphological description of the female stage of O. peropteryx. In addition, the larvae were used for a morphological redescription of this stage. One larva and two legs extirpated from the adult female were submitted to DNA extraction and PCR targeting a fragment of the mitochondrial 16S rDNA gene, which yielded DNA sequences at least 11 % divergent from any available argasid sequence in Genbank. We show that O. peropteryx ontogeny is characterized by a single, non-feeding, nymphal stage. This condition has never been reported for ticks.
      
      

    • Ornithodoros guaporensis (Acari, Ixodida: Argasidae), a new tick species from the Guaporé River Basin in the Bolivian Amazon (Completo, 2013)

      NAVA, S. , VENZAL, J.M. , TERASSINI, F.A. , MANGOLD, A.J. , CAMARGO, L.M.A. , CASÁS, G. , LABRUNA, M.B.
      Zootaxa, v.: 3666 4 , p.:579 - 590, 2013
      Palabras clave: Argasidae Ornithodoros guaporensis Bolivia Gauporé River Basin
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Internet
      ISSN: 11755326
      E-ISSN: 11755334
      DOI: 10.11646/zootaxa.3666.4.10
      The soft tick Ornithodoros guaporensis n. sp. (Acari: Ixodida: Argasidae) is described from larvae and adults. Morphological analysis and 16S rDNA sequences are provided. Adults were collected from a rocky fissure inhabited by bats located in the Amazonian forest in north-eastern Bolivia (Beni Department) close to the Guaporé River. Larvae were obtained from eggs laid by females collected in the field, and which were fed on rabbits in the laboratory. Larvae of O. guaporensis are morphologically closely related to Ornithodoros rioplatensis, Ornithodoros puertoricensis and Ornithodoros talaje. Larvae of O. guaporensis and O. rioplatensis can be separated from O. puertoricensis and O. talaje by the number of pairs of dorsal setae (20 in O. guaporensis and O. rioplatensis, 18 in O. puertoricensis and 17 in O. talaje). Larvae of O. guaporensis and O. rioplatensis can be differentiated by the medial dental formula (2/2 in O. guaporensis and 3/3 in O. rioplatensis) and the apex of the hypostome, which is more pointed in O. rioplatensis than in O. guaporensis. The Principal Component Analysis performed with morphometric characters of larvae showed a clear separation among O. guaporensis, O. rioplatensis, O. puertoricensis and O. talaje. Significant morphological differences among adults of these four species were not found. The analysis of the 16S rDNA sequences allowed for the differentiation between O. guaporensis and the remaining Neotropical species of the family Argasidae.
      
      

    • Sequence analysis of the internal transcribed spacer 2 (ITS2) from Philornis seguyi (García, 1952) and Philornis torquans (Nielsen, 1913) (Diptera: Muscidae). (Completo, 2013)

      MONJE, L.D. , QUIROGA, M. , MANZOLI, D. , COURI, M.S. , SILVESTRI, L. , VENZAL, J.M. , CUERVO, P. , BELDOMÉNICO, P.M.
      Systematic Parasitology, v.: 86 p.:43 - 51, 2013
      Palabras clave: Avian ectoparasite Philornis torquans Philornis downsi Philornis seguyi Internal transcribed spacer 2
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Papel
      ISSN: 01655752
      E-ISSN: 15735192
      DOI: 10.1007/s11230-013-9428-5
      Philornis Meinert, 1890 (Diptera: Muscidae) is a genus of Neotropical dipterans that parasitise birds. The currently used external morphological characters to distinguish between species within this genus present some limitations. We used the second internal transcribed spacer region (ITS2) of the rRNA gene as a molecular marker to differentiate adult specimens of Philornis identified morphologically as P. torquans and P. seguyi from different localities. Specimens identified as P.seguyi from Magdalena (Buenos Aires Province) showed an ITS2 sequence different from that for P. torquans, whereas all other specimens of P. seguyi had sequences identical to those for P. torquans. These findings do not necessarily confirm that specimens from Magdalena indeed belong to P. seguyi, nor that P. seguyi is a valid species. Instead, they alert us about the potential for species misidentification when using morphological characters alone. The use of molecular approaches to aid the identification of Philornis spp. Will shed light on the systematics of this group. Philornis torquans is reported for the first time in Mendoza Province and Uruguay.
      
      

    • Epidemiología de rickettsiosis por Rickettsia parkeri y otras especies emergentes o re-emergentes asociadas a la antropización en Latinoamérica (Completo, 2013)

      VENZAL, J.M.
      Acta Médica Costarricense, v.: 55 supl. 1 , p.:45 - 47, 2013
      Palabras clave: Rickettsia zoonosis Infecciones por Rickettsiaceae América Latina
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas / Rickettsiosis
      Medio de divulgación: Internet
      ISSN: 00016012
      E-ISSN: 22155856
      Se describe la importancia regional de Rickettsia parkeri y sus respectivos vectores. Se hace énfasis en los factores de antropización que favorecen la aparición de hospedadores alternativos para las garrapatas en los entornos domésticos y peridomésticos, generando modificaciones en la epidemiología del agente etiológico. También se menciona las modificaciones ecológicas que pueden favorecen el incremento de poblaciones de reservorios para las garrapatas incrementando el riesgo para el ser humano de sufrir enfermedades rickettsiales.
      
      

    • Immature argasid ticks: diagnosis and keys for Neotropical region (Completo, 2013)

      BARROS-BATTESTI, D.M. , GARCIA RAMIREZ, D. , LANDULFO, G.A. , FACCINI, J.L.H. , DANTAS-TORRES, F. , LABRUNA, M.B. , VENZAL, J.M. , ONOFRIO, V.C.
      Revista Brasileira De Parasitologia Veterinaria, v.: 22 4 , p.:443 - 456, 2013
      Palabras clave: Argasidae inmaduros clave identificación Brasil
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Papel
      ISSN: 0103846X
      E-ISSN: 19842961
      DOI: 10.1590/S1984-29612013000400002
      Barros-Battesti, D.M.; Garcia Ramirez, D.; Landulfo, G.A.; Faccini, J.L.H.; Dantas-Torres, F.; Labru
      
      

    • Case Report: A Confirmed Case of Rickettsia parkeri Infection in a Traveler from Uruguay (Completo, 2013)

      PORTILLO, A. , GARCÍA-GARCÍA, C. , SANZ, M.: , SANTIBÁÑEZ, S. , VENZAL, J.M. , OTEO, J.A.
      American Journal of Tropical Medicine and Hygiene, v.: 89 6 , p.:1203 - 1205, 2013
      Palabras clave: Uruguay Rickettsia parkeri Rickettsiosis
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Medio de divulgación: Papel
      ISSN: 00029637
      E-ISSN: 14761645
      DOI: 10.4269/ajtmh.13-0436
      García-García, C.; Mercedes Sanz, M.; Santibáñez, S.; Venzal, J.M.; Oteo, J.A.
      
      

    • Mixosporidiosis branquial por Henneguya sp. (Myxozoa: Myxobolidae) en sábalos (Pisces: Prochilodontidae) del Río Uruguay y Río de la Plata. (Completo, 2013)

      CARNALES, D. , CARNEVIA, D. , PERRETTA, A. , VENZAL, J.M.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 49 192 , p.:4 - 14, 2013
      Palabras clave: Río Uruguay Henneguya sp. branquias Prochilodus lineatus Río de la Plata
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03764362
      E-ISSN: 16884809
      Existen numerosos mixosporidios parásitos de peces, dentro de los cuales el género Henneguya ha sido descrito parasitando al sábalo (Prochilodus lineatus) en el Río Paraná por varios autores en Brasil. El objetivo del presente trabajo fue identificar la o las especies de Henneguya presentes en branquias de sábalos del Río Uruguay y Río de la Plata, estableciendo además la prevalencia y lesiones histopatológicas de la parasitosis. Se colectaron 81sábalos de los cuales 40 resultaron parasitados, lo que supone una prevalencia de 49,4%. Los peces parasitados presentaron en las branquias pequeños quistes ovoideos de 0,5-1 mm de diámetro mayor. Dentro de estos quistes se reconoció la presencia de mixosporas de Henneguya sp. caracterizadas por poseer una forma oval, dos cápsulas polares, una vacuola iodófila y un proceso caudal bifurcado. Las medidas registradas fueron: 34,80 ± 4,23 μm largo total; 14,04 ± 1,96 μm largo del cuerpo; 5,53 ± 0,81 μm ancho del cuerpo; 7,25 ± 1,03 μm largo de la cápsula polar y 2,79 ± 1,27 μm ancho de la cápsula polar. A la histopatología se identificó que los plasmodios se localizan en la zona interlaminillar de las láminas secundarias produciendo escasa alteración y respuesta del huésped. Las especies de Henneguya descritas en sábalos del Río Paraná (H. caudalongula, H. paranaensis y Henneguya sp.) presentan características morfométricas diferentes de la encontrada en este trabajo, por lo que postulamos que podría tratarse de una especie diferente de Henneguya la que parasita al sábalo en el Río Uruguay y Río de la Plata. Estos parásitos podrían utilizarse como indicadores biológicos de poblaciones de sábalos en la cuenca del Plata.
      
      

    • Maternal Care in the Soft Tick Antricola marginatus (Completo, 2012)

      LABRUNA, M.B. , NAVA, S. , GUZMÁN-CORNEJO, C. , VENZAL, J.M.
      Journal of Parasitology, v.: 98 4 , p.:876 - 877, 2012
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Papel
      ISSN: 00223395
      E-ISSN: 19372345
      Among spiders, scorpions, and whip spiders, a common type of maternal care consists of females carrying newly hatched offspring on their body for a few days, until the offspring is able to live independently. While this maternal care has been suggested to occur in different argasid tick species, it has been recorded only once, for Antricola marginatus in Cuba; however, this earlier record only superficially mentioned the occurrence of this behavior, with no further details. Here, we report the occurrence of maternal care in the argasid tick A. marginatus under natural conditions in a cave at Yucatan, Mexico, where 8 A. marginatus females, while walking on bat guano, had their body entirely covered by a mean number of 305 ± 112 conspecific unfed larvae (range: 105-466). Larvae covered the entire idiosoma of the female tick, where they were motionless or displaying slight movements. This result substantially expands the number of unique characters that have been found only in Antricola spp. ticks, when compared to the other tick genera. Our findings also indicate that maternal care evolved independently in different taxa of Arachnida, because it has been reported for species of Araneae, Scorpiones, and Amblypygi, and here for an Acari species.
      
      

    • Primer reporte de parasitismo de una garrapata blanda del género Ornithodoros (Ixodida: Argasidae) sobre Rhinella arenarum (Anura: Bufonidae) en el departamento de Valle Fértil, San Juan, Argentina. (Completo, 2012)

      GONZALEZ RIVAS, C.J. , CASTILLO, G.N. , ACOSTA, J.C. , VENZAL, J.M. , GUGLIELMONE, A.A.
      Cuadernos de Herpetología, v.: 26 2 , p.:95 - 97, 2012
      Palabras clave: Argentina Ornithodoros Rhinella arenarum
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Papel
      ISSN: 0326551X
      E-ISSN: 18525768
      
      

    • Ornithodoros peruvianus Kohls, Clifford & Jones (Ixodoidea: Argasidae) in Chile, a Tentative Diagnosis (Completo, 2012)

      VENZAL, J.M. , GONZÁLEZ-ACUÑA, D. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Neotropical Entomology, v.: 41 1 , p.:74 - 78, 2012
      Palabras clave: Chiroptera 16S sequence Desmodus rotundus Ornithodoros peruvianus
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Acarología
      Medio de divulgación: Papel
      ISSN: 1519566X
      E-ISSN: 16788052
      Three argasid tick larvae were collected on April 2, 2010 from a common vampire bat, Desmodus rotundus, captured in the Parque Nacional Pan de Azúcar (26º09´S 70º41´W), Region of Atacama, Chile. The larvae were diagnosed as Ornithodoros and further comparative analysis showed them to be O. peruvianus Kohls, Clifford & Jones or a species close to it. Phylogenetic analysis based on 16S mitochondrial rRNA gene sequences of Ornithodoros species plus four Argas, species were carried out to clarify the taxonomic position of the larvae. This is the first finding of ticks parasitizing D. rotundus in Chile.
      
      

    • Morphological study of Ornithodoros viguerasi Cooley and Kohls, 1941 (Acari: Ixodida: Argasidae), with sequence information from the mitochondrial 16S rDNA gene (Completo, 2012)

      NAVA, S. , VENZAL, J.M. , REYES NOVELO, E.A. , MANGOLD, A.J. , LABRUNA, M.B.
      Acarologia, v.: 52 1 , p.:29 - 38, 2012
      Palabras clave: Argasidae Morphology Ornithodoros viguerasi 16S rDNA Mexico
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Papel
      ISSN: 0044586X
      E-ISSN: 21077207
      DOI: 10.1051/acarologia/20122035
      A morphological and molecular study of Ornithodoros (Subparmatus) viguerasi (Acari: Argasidae) was carried out. Free-living males and females of this tick species were collected in Calcehtok cavern, Yucatán, Mexico. The morphology of the females of O. viguerasi was identical to the holotype female from Cuba. The only difference was related to size; females fromMexico were bigger than the holotype female. The male of O. viguerasi was described for the first time from the specimens collected in Mexico. The diagnostic characters are a combination of a genital aperture covered by a semicircular flap, a central sclerotized plate posterior to genital aperture, a transverse and thin plate anterior to the genital aperture located at the level of the anterior margin of coxae I, a pair of sclerotized plates bordering coxae II, III and IV, basis capituli rectangular in shape and protrusible, a hypostome roughly blunt at the apex with small denticles (it appears to be functionless), and article I of the palpi with a medial integumental extension which has a long setae on the medial margin. A comparative morphological analysis of the male described in this work with the paratype specimens from Cuba was also conducted; we conclude that the nymphs described in the original description of O. viguerasi correspond to males. Sequences of the mitochondrial 16S rDNA gene of both male and female ticks from Mexico were identical. In a phylogenetic analysis, the monophyly of the subgenus Subparmatus could not be resolved.
      
      

    • Description of a new species of bat-associated argasid tick (Acari: Argasidae) from Brazil. (Completo, 2012)

      DANTAS-TORRES, F. , VENZAL, J.M. , BERNARDI, L.F.O.I , FERREIRA, R.L. , ONOFRIO, V.C. , MARCILI, A. , BERMÚDEZ, S.E. , RIBEIRO, A.F. , BARROS-BATTESTI, D.M. , LABRUNA, M.B.
      Journal of Parasitology, v.: 98 1 , p.:36 - 45, 2012
      Palabras clave: Ticks Brazil Ornithodoros New species
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Acarología
      Medio de divulgación: Papel
      ISSN: 00223395
      E-ISSN: 19372345
      A new species of argasid tick is described from immature and adult specimens collected from several localities in Brazil. A complete morphological account is provided for all postembryonic life stages (i.e., larva, nymph, female, and male). The new species belongs to the genus Ornithodoros, which now includes 113 species. Morphologically, the new species shares common features (e.g., presence of well-developed cheeks and legs with micromammillate cuticle) with other bat-associated argasid ticks included in the subgenus Alectorobius. In particular, the new species is morphologically related to Ornithodoros azteci Matheson, with which it forms a species group. Phylogenetic analysis based on the 16S rRNA gene sequences supports the placement of the new species within a large clade that includes other New World bat-associated argasids. However, the new species appears to represent an independent lineage within the genus Ornithodoros.
      
      

    • Mitochondrial DNA analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) in the Southern Cone of South America (Completo, 2012)

      NAVA, S. , MASTROPAOLO, M. , VENZAL, J.M. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Veterinary Parasitology, v.: 190 p.:547 - 555, 2012
      Palabras clave: SOUTH AMERICA 16S rDNA Rhipicepahlus sanguineus sensu lato 12S rDNA Genetic analysis
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03044017
      A genetic analysis of partial sequences of the mitochondrial 16S and 12S rDNA genes of Rhipicephalus sanguineus sensu lato collected in the Southern Cone of South America was carried out. Also, sequences of ticks belonging to this taxon from Europe, Africa and other South American localities were included. TCS networks constructed with 16S rDNA sequences showed two clusters of haplotypes, namely, Southern lineage (ST) and Northern lineage (NT). Haplotypes representing the specimens coming from localities of Argentina, Uruguay and Chile were included in the ST lineage, while haplotypes from Brazil, Paraguay, Colombia, South Africa, Mozambique and from two localities of Northern Argentina were grouped in the NT lineage. The phylogenetic trees obtained with both 16S and 12S sequences showed two distinct clades, one containing R. sanguineus s.l from Argentina, Uruguay, Chile (ST lineage) and Western Europe (Italy and France), and a second clade including R. sanguineus s.l from Argentina, Paraguay, Brazil, Colombia (NT lineage), South Africa and Mozambique. The results herein reported revealed that the taxon R. sanguineus s.l is represented by two lineages in the Southern Cone of South America. According with the genetic comparative analysis, NT lineage and the ticks from Mozambique and South Africa represent a species that is not R. sanguineus s.s, while R. sanguineus s.l ticks from Western Europe and Southern South America (ST lineage) probably represent true R. sanguineus, because the type locality of R. sanguineus s.s is located in France. The taxonomic issue described for R. sanguineus s.l in the South America has epidemiological implications. Difference in the vectorial competence for Ehrlichia canis between the two lineages of R. sanguineus s.l was found in previous works. Further investigations are needed in order to verify a possible different vectorial competence for the other pathogens transmitted by these ticks.
      
      

    • Rickettsia parkeri: a rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern Uruguay (Completo, 2012)

      VENZAL, J.M. , ESTRADA-PEÑA, A. , PORTILLO, A. , MANGOLD, A.J. , CASTRO, O. , DE SOUZA, C.G. , FÉLIX, M.L. , PÉREZ-MARTÍNEZ, L. , SANTIBÁÑEZ, S. , OTEO, J.A.
      Revista do Instituto de Medicina Tropical de São Paulo, v.: 54 3 , p.:131 - 134, 2012
      Palabras clave: Uruguay Rhipicephalus sanguineus Rickettsia parkeri Amblyomma triste fiebres manchadas
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Medio de divulgación: Papel
      E-ISSN: 00364665
      Santibánez, S.; Oteo, J.A. 2012.
      At first Rickettsia conorii was implicated as the causative agent of spotted fever in Uruguay diagnosed by serological assays. Later Rickettsia parkeri was detected in human biting Amblyomma triste ticks using molecular tests. The natural vector of R. conorii, Rhipicephalus sanguineus, has not been studied for the presence of rickettsial organisms in Uruguay. To address this question, 180 R. sanguineus from dogs and 245 A. triste from vegetation (flagging) collected in three endemic localities were screened for spotted fever group (SFG) rickettsiosis in southern Uruguay. Tick extracted DNA pools were subjected to PCR using primers which amplify a fragment of the rickettsial gltA gene. Positive tick DNA pools with these primers were subjected to a second PCR round with primers targeting a fragment of the ompA gene, which is only present in SFG rickettsiae. No rickettsial DNA was detected in R. sanguineus. However, DNA pools of A. triste were found to be positive for a rickettsial organism in two of the three localities, with prevalences of 11.8% to 37.5% positive pools. DNA sequences generated from these PCR-positive ticks corresponded to R. parkeri. These findings, joint with the aggressiveness shown by A. triste towards humans, support previous data on the involvement of A. triste as vector of human infections caused by R. parkeri in Uruguay.
      
      

    • Molecular identification and description of the female of Nothoaspis reddelli (Ixodida: Argasidae) from a cave in Southeastern Mexico (Completo, 2012)

      GUZMÁN-CORNEJO, C. , PAREDES-LEÓN, R. , LABRUNA, M.B. , NAVA, S. , VENZAL, J.M.
      Journal of Parasitology, v.: 98 5 , p.:918 - 923, 2012
      Palabras clave: Argasidae bats Mexico Nothoaspis reddelli
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Papel
      ISSN: 00223395
      E-ISSN: 19372345
      Nothoaspis reddelli Keirans and Clifford, 1975 was described from 3 males collected in Grutas de Xtacumbilxunaán, Campeche, Mexico, although females have remained undescribed for 36 yr. Recently, adult females of this species were collected from Cueva de Villa Luz (= Cueva de las Sardinas, Cueva del Azufre), in Tapijulapa, Tabasco, Mexico. Here, we present a morphological description of the female stage, together with 16S rDNA sequences that confirm the conspecificity of our female, male, and nymphal specimens. The female integument of the anterior portion of the dorsal surface is smooth (nothoaspis), appearing to consist of 3 large “subunits”, 1 anterior and 2 posterior, each with a small sublateral “subunit” on either side. The remaining dorsal covered integument is a cell-like configuration. The hood is large and bluntly rounded, and visible dorsally. The spiracular plate is oval. It possesses 1 pair of posthypostomal setae. The palpal trochanter has 1 pair of setae, and a 5/5 hypostome decreasing to 4/4 at the apex. There is a single central pore at the base of the hypostome.
      
      

    • Reinstatement of Rhipicephalus (Boophilus) australis With Redescription of the Adult and Larval Stages (Completo, 2012)Trabajo relevante

      ESTRADA-PEÑA, A. , VENZAL, J.M. , NAVA, S. , MANGOLD, A.J. , GUGLIELMONE, A.A. , LABRUNA, M.B. , DE LA FUENTE, J.
      Journal of Medical Entomology, v.: 49 4 , p.:794 - 802, 2012
      Palabras clave: Rhipicephalus (Boophilus) australis Australian cattle tick reinstatement redescription
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00222585
      E-ISSN: 19382928
      DOI: 10.1603/ME11223
      Rhipicephalus australis Fuller, the Australian cattle tick, is reinstated and the adults and larvae redescribed from material collected in Australia. This long ignored boophilid was previously known as R. microplus Canestrini for specimens reported in Australia and New Caledonia. The adults of R. australis are easily recognized by a combination of characters, such as the ventro-medial spurs in the palpal segments of the male, and the abundant, plumose, pale white setae on the dorsum of the female. Other details, such as coxal and adanal shields are more variable among different populations and may lead to incorrect determinations. Larvae of R. australis are clearly smaller than those of R. microplus. The use of principal components analysis on body measurements leads to a clear separation of larvae of both taxa. A phylogenetic analysis based on 12S- and 16S-rDNA gene sequences supports the conspeciÞcity of the neotype material on which the reinstatement of the species is proposed, and of the specimens used for previous interspeciÞc crosses. R. australis is now known to be present in Australia, New Caledonia, the island of Borneo, Philippines, Sumatra, Java, New Guinea, Cambodia, and Tahiti. Both R. microplus and R. australis coexist in some countries in southeastern Asia. Given the extreme importance of these ticks for the cattle industry, Þeld data on their distribution in the region are required to know the actual range of these species and to understand the evolution of the group.
      
      

    • Ornithodoros quilinensis sp. nov. (Acari, Argasidae), a new tick species from the Chacoan region in Argentina (Completo, 2012)

      VENZAL, J.M. , NAVA, S. , MANGOLD, A.J. , MASTROPAOLO, M. , CASÁS, G. , GUGLIELMONE, A.A.
      Acta Parasitologica, v.: 57 3 , p.:329 - 336, 2012
      Palabras clave: Argasidae Argentina Ornithodoros quilinensis Chacoan region
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Papel
      ISSN: 12302821
      E-ISSN: 18961851
      DOI: 10.2478/s11686-012-0034-5.
      Ornithodoros quilinensis sp. nov. (Acari: Argasidae) is described from larvae collected on the small rodents Graomys centralis (Cricetidae: Sigmodontinae) in Argentina. The diagnostic characters for this new species are a combination of small size (520–540 μm), a dorsal plate oval in shape with a length of approximately 200 μm, 14 pairs of dorsal setae, hypostome short and narrower at the base (length from Ph1 to apex 133 μm (120–141)) with dental formula 2/2 and apex blunt, and the capsule of the Haller’s organ irregular in shape and without reticulations. The analysis of the 16S rDNA sequences available for the genus Ornithodoros indicate that, phylogenetically, O. quilinensis represents an independent lineage only related to a Bolivian tick species of the genus Ornithodoros yet not formally described.
      
      

    • Primer registro del piojo Heterodoxus spiniger (Phthiraptera: Amblycera: Boopidae) parasitando perros en Uruguay. (Completo, 2012)

      VENZAL, J.M. , RADCENCO, P. , ROCCA, H. , SEQUEIRA, C.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 48 187 , p.:21 - 23, 2012
      Palabras clave: Uruguay Heterodoxus spiniger primer registro perros
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 03764362
      E-ISSN: 16884809
      Se reporta por primera vez al piojo Heterodoxus spiniger parasitando perros en Uruguay, el hallazgo se realizó en la ciudad de Bella Unión en el Departamento de Artigas. También se confirma la presencia de Linognathus setosus el cual no estaba documentado bibliográficamente para el país. Con el presente reporte son tres las especies de piojos hallados parasitando perros en Uruguay: Trichodectes canis, H. spiniger y L. setosus.
      
      

    • Description of adults, nymph and redescription of the larva of Ornithodoros marinkellei (Acari: Argasidae), with data on its phylogenetic position. (Completo, 2011)

      LABRUNA, M.B. , NAVA, S. , TERASSINI, F. , ONOFRIO, V.C. , BARROS-BATTESTI, D.M. , CAMARGO, L.M.A. , VENZAL, J.M.
      Journal of Parasitology, v.: 97 2 , p.:207 - 217, 2011
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00223395
      E-ISSN: 19372345
      DOI: 10.1645/GE-2620.1
      The argasid tick Ornithodoros marinkellei Kohls, Clifford, and Jones, 1969 was described 4 decades ago based on larval specimens collected from bats (Pteronotus spp.) in Colombia and Panama. Thereafter, larval O. marinkellei parasitizing bats were reported from Venezuela, Guyana, and Brazil. Herein, we describe the adults and nymph, and redescribe the larva of O. marinkellei based on specimens recently collected in the western Brazilian Amazon region. In contrast to all other known adult argasids, the idiosoma of both males and females of O. marinkellei is covered with sclerotized plaques. The idiosoma of the nymph of O. marinkellei is entirely micromamillated, and differs from the adults by the absence of plaques. The larva of O. marinkellei is morphologically similar to the larvae of the 2 other species belonging to the subgenus Subparmatus, i.e, Ornithodoros viguerasi Cooley and Kohls, 1941 and Ornithodoros mormoops Kohls, Clifford, and Jones, 1969. Because of the long and narrow dorsal plate, the larva of O. marinkellei is readily distinguished from O. viguerasi and O. mormoops. Comparison of our larvae from Brazil with O. marinkellei paratype specimens from Colombia confirmed their taxonomic identification. However, a few morphological differences, particularly in the size of the gnathosoma, were observed. Further studies are necessary to clarify whether O. marinkellei is a complex of different species, or a single species represented by morphologically polymorphic, and geographically distinct populations. Partial mitochondrial 16S rDNA gene sequences were generated for O. marinkellei specimens from Brazil, and compared with available homologous sequences in GenBank. Phylogenetic analyses revealed O. marinkellei to be distinct from the remaining argasid species available in GenBank, including other bat-associated tick species that are found in sympatry with O. marinkellei in the Neotropical region.
      
      

    • Carios mimon (Acari: Argasidae): description of adults and redescription of larva (Completo, 2011)

      BARROS-BATTESTI, D.M. , LANDULFO, G.A. , ONOFRIO, V.C. , FACCINI, J.L.H. , MARCILI, A. , NIERI-BASTOS, F.A. , VENZAL, J.M. , LABRUNA, M.B.
      Experimental and Applied Acarology, v.: 54 p.:93 - 104, 2011
      Palabras clave: mitochondrial 16S rDNA sequence Carios mimon Argasid Adults Larva Morphology
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      DOI: 10.1007/s10493-010-9416-2
      Carios mimon is an argasid tick common on Chiroptera, originally described from larvae collected on bats Mimon crenulatum from Bolivia and Eptesicus brasiliensis from Uruguay. Later it was also registered from Argentina and recently included among the Brazilian tick fauna. In Brazil, this species is very aggressive to man, resulting in intense inflammatory response and pain. It is known only by the larval description and its morphology resembles that from other species currently included into the genus Carios, formerly classified into the subgenus Alectorobius, genus Ornithodoros. Here we describe adults and redescribe the larva of C. mimon, based on light and scanning electron microscopy. Remarks about its morphological similarity with other species of this genus are also discussed. Molecular analysis inferred from a portion of the 16S rRNA mitochondrial gene placed C. mimon in a cluster supported by maximal bootstrap value (100%) with other argasid species (mostly bat parasites in the New World), which have been classified into either the genus Ornithodoros or Carios, depending on the Argasidae classification adopted by different authors.
      
      

    • Seasonal dynamics and hosts of Amblyomma triste (Acari: Ixodidae) in Argentina (Completo, 2011)

      NAVA, S. , MANGOLD, A.J. , MASTROPAOLO, M. , VENZAL, J.M. , FRACASSI, N. , GUGLIELMONE, A.A.
      Veterinary Parasitology, v.: 181 p.:301 - 308, 2011
      Palabras clave: Amblyomma triste Argentina Hosts ecology
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Parasitología /
      Medio de divulgación: Papel
      ISSN: 03044017
      The seasonal dynamics and host usage of Amblyomma triste in Argentina were analyzed. Adults of A. triste were present from early winter to mid-summer, with the peak of abundance from late winter to mid-spring (August to October). Larvae and nymphs were found from December to June, with the peak of abundance in summer. There were no differences among the biological parameters (pre-moult period of larvae and nymphs, pre-oviposition period of females, and minimum incubation period of eggs) of engorged tick sex posed to different photoperiod regimens at the laboratory, but the periods for each biological parameter obtained from tick sex posed in the field were significantly longer than those from the laboratory. Field results fit better with the data of seasonal distribution of each stage. Morphogenetic diapause was not detected, but complementary studies should test the presence of behavioural diapause. Rodents of the subfamily Sigmodontinae (Akodon azarae, Oligoryzomys flavescens, Oligoryzomys nigripes, Oxymycterus rufus and Scapteromys aquaticus) are the principal hosts for immature stages of A. triste, the caviid Cavia aperea could be another potential host for these stages, and birds are exceptional hosts for larvae and nymphs. Regarding hosts of adults in Argentina, domestic and wild large-sized mammals belonging to different orders (cattle, dog, horse, Blastocerus dichotomus and Hydrochoerus hydrochaeris) serve as hosts for adults of this tick species. In conclusion, A. triste has a life cycle of 1year with adults feeding on large endemic and introduced mammals and immature stages using sigmodontine and caviid rodents as hosts.
      
      

    • Distribution and 16s rDNA sequences of Argas monachus (Acari: Argasidae), a soft tick parasite of Myiopsitta monachus (Aves: Psittacidae). (Completo, 2011)

      MASTROPAOLO, M. , TURIENZO, P. , DI IORIO, O. , NAVA, S. , VENZAL, J.M. , GUGLIELMONE, A.A. , MANGOLD, A.J.
      Experimental and Applied Acarology, v.: 55 p.:283 - 291, 2011
      Palabras clave: distribution Ticks Argentina Argas monachus Myiopsitta monachus DNA sequences
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      Specimens of Argas monachus Keirans et al. were collected from Myiopsitta monachus nests in 42 localities in Argentina and Paraguay from 2006 to 2010. A list of localities where this tick has been found is presented. 16S rDNA sequences of specimens of A. monachus from different localities were compared to confirm whether they belong to the same specific taxon. Argas monachus is present in the phytogeographic provinces of Chaco, Espinal, and Monte, but not in the Pampa (all from de Chaco Domain) where the host is well distributed. No differences were found among 16S rDNA sequences of geographically distant specimens.
      
      

    • El género Rickettsia como agente de zoonosis en el Cono Sur de Sudamérica (Completo, 2011)

      VENZAL, J.M. , NAVA, S.
      Revista Médica del Uruguay, v.: 27 p.:98 - 106, 2011
      Palabras clave: Rickettsia zoonosis
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Medio de divulgación: Papel
      ISSN: 03033295
      E-ISSN: 16880390
      27: 98-106
      Las rickettsiosis son zoonosis causadas por bacterias del género Rickettsia que se clasifican en dos grupos: el de las fiebres manchadas y el de las fiebres tíficas. Las fiebres manchadas son en su mayoría transmitidas por garrapatas, aunque algunas son transmitidas por pulgas y ácaros. En las fiebres tíficas los vectores primarios son el piojo humano del cuerpo y la pulga Xenopspylla cheopis. Hasta finales del siglo XX las únicas rickettsiosis reconocidas en Sudamérica eran Rickettsia prowazekii, Rickettsia typhi (fiebres tíficas) y Rickettsia rickettsii (fiebres manchadas). Pero en la última década, mediante el empleo de modernas técnicas de diagnóstico, se han determinado en Sudamérica varias especies o cepas de rickettsias del grupo de las fiebres manchadas, algunas de las cuales son patógenas para humanos. En el Cono Sur de Sudamérica, seis especies de Rickettsia y al menos cuatro cepas de Rickettsia sp. fueron detectadas en garrapatas y una en pulgas. Cuatro especies de estas rickettsias, R. rickettsii, Rickettsia parkeri, Rickettsia massiliae y Rickettsia felis y una cepa de Rickettsia sp. (cepa Mata Atlántica) son reconocidas como patógenas para los humanos. En este trabajo se describen los casos clínicos causados por las tres principales especies de rickettsias con importancia epidemiológica en el Cono Sur de Sudamérica: R. rickettsii, R. parkeri y R. massiliae. La información del trabajo comprende Argentina, la mitad meridional de Brasil, Chile y Uruguay. Paraguay no se incluye debido a la falta absoluta de estudios sobre rickettsias.
      
      

    • Rickettsioses in Latin America, Caribbean, Spain and Portugal (Completo, 2011)

      LABRUNA, M.B. , MATTAR, S. , NAVA, S. , BERMÚDEZ, S.E. , VENZAL, J.M. , DOLZ, G. , ABARCA, K. , ROMERO, L. , DE SOUZA, R. , OTEO, J.A. , ZAVALA-CASTRO, J.
      Revista Mvz Córdoba, v.: 16 2 , p.:2435 - 2457, 2011
      Palabras clave: Acari epidemiology rocky mountain spotted fever vector control
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas / Rickettsiosis
      Medio de divulgación: Papel
      ISSN: 01220268
      E-ISSN: 19090544
      Data on genus and infectious by Rickettsia were retrospectively compiled from the critical review literature regarding all countries in Latin America, Caribbean islands, Portugal and Spain. We considered all Rickettsia records reported for human and/or animal hosts, and/or invertebrate hosts considered being the vector. In a few cases, when no direct detection of a given Rickettsia group or species was available for a given country, the serologic method was considered. A total of 13 Rickettsia species have been recorded in Latin America and the Caribbean. The species with the largest number of country confirmed records were Rickettsia felis (9 countries), R. prowazekii (7 countries), R. typhi (6 countries), R. rickettsii (6 countries), R. amblyommii (5 countries), and R. parkeri (4 countries). The rickettsial records for the Caribbean islands (West Indies) were grouped in only one geographical area. Both R. bellii, R. akari, and Candidatus ‘R. andeane’ have been recorded in only 2 countries each, whereas R. massiliae, R. rhipicephali, R.monteiroi, and R. africae have each been recorded in a single country (in this case, R. africae has been recorded in nine Caribbean Islands). For El Salvador, Honduras, and Nicaragua, no specific Rickettsia has been reported so far, but there have been serological evidence of human or/and animal infection. The following countries remain without any rickettsial records: Belize, Venezuela, Guyana, Surinam, and Paraguay. In addition, except for a few islands, many Caribbean islands remain without records. A total of 12 Rickettsia species have been reported in Spain and Portugal: R. conorii, R. helvetica, R. monacensis, R. felis, R. slovaca, R. raoultii, R. sibirica, R. aeschlimannii, R. rioja, R. massiliae, R. typhi, and R. prowazekii. Amongst these Rickettsia species reported in Spain and Portugal, only R. prowazekii, R. typhi, R. felis, and R. massiliae have also been reported in Latin America. This study summarizes the current state of art on the rickettsial distribution in Latin America, Caribbean, Spain and Portugal. The data obtained allow a better understanding on rickettsial epidemiology and distribution of vector ecology.
      
      

    • Seroprevalencia de la fasciolosis bovina en el departamento de Salto (Uruguay) (Completo, 2011)

      SANCHÍS, J. , MIGUÉLEZ, S. , SOLARI, M.A. , PIÑEIRO, P. , MACCHI, M.I. , MALDINI, G , VENZAL, J.M. , MORRONDO, P. , DíEZ-BAñOS, P. , SÁNCHEZ-ANDRADE, R. , PAZ-SILVA, A. , ARIAS, M.S.
      Revista Ibero-latinoamericana de Parasitología, v.: 70 2 , p.:163 - 171, 2011
      Palabras clave: Uruguay ELISA fasciolosis bovine herd
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Internet
      ISSN: 07188730
      A serological survey to gain more knowledge about bovine fasciolosis in Salto Department (Uruguay) by using an ELISA and excretory/secretory antigens was conducted. Blood samples were collected from 386 cattle from beef, dairy and/or mixed farms. Data obtained were analyzed regarding intrinsic (age, breed and gender) and extrinsic factors (aptitude). The relationship among these variables and the results was demonstrated by the estimation of odds ratio (OR) and prevalence ratio (PR) values. The cases were classified according the CHAID (Chi Squared Automatic Interaction Detection) test. An overall individual seroprevalence of 67% (IC95% 62, 72) was obtained, and the highest percentages in Angus cattle and younger than 2 yr were observed. Breed resulted the most influent factor, and then the age.Ninety-two percent (CI 95% 78, 100) of the bovine herds had ruminants positive to the ELISA.It was concluded that fasciolosis is a very important problem in the bovine farms from Uruguay, which underlines the need for the application of measures against the intermediate and definitive hosts to reduce its appearance.
      
      

    • Hosts, distribution and genetic divergence (16S rDNA) of Amblyomma dubitatum (Acari: Ixodidae) (Completo, 2010)

      NAVA, S. , VENZAL, J.M. , LABRUNA, M.B. , MASTROPAOLO, M. , GONZÁLEZ, E.M. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Experimental and Applied Acarology, v.: 51 4 , p.:335 - 351, 2010
      Palabras clave: distribution Hosts Amblyomma dubitatum Biogeography Genetic divergence 16S rDNA sequences
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      DOI: 10.1007/s10493-009-9331-6
      We supply information about hosts and distribution of Amblyomma dubitatum. In addition, we carry out an analysis of genetic divergence among specimens of A. dubitatum from different localities and with respect to other Neotropical Amblyomma species, using sequences of 16S rDNA gene. Although specimens of A. dubitatum were collected on several mammal species as cattle, horse, Tapirus terrestris, Mazama gouazoubira, Tayassu pecari, Sus scrofa, Cerdocyon thous, Myocastor coypus, Allouata caraya, Glossophaga soricina and man, most records of immature and adult stages of A. dubitatum were made on Hydrochoerus hydrochaeris, making this rodent the principal host for all parasitic stages of this ticks. Cricetidae rodents (Lundomys molitor, Scapteromys tumidus), opossums (Didelphis albiventris) and vizcacha (Lagostomus maximus) also were recorded as hosts for immature stages. All findings of A. dubitatum correspond to localities of Argentina, Brazil, Paraguay and Uruguay, and they were concentrated in the Biogeographical provinces of Pampa, Chaco, Cerrado, Brazilian Atlantic Forest, Parana Forest and Araucaria angustifolia Forest. The distribution of A. dubitatum is narrower than that of its principal host, therefore environmental variables rather than hosts determine the distributional ranges of this tick. The intraspecific genetic divergence among 16S rDNA sequences of A. dubitatum ticks collected in different localities from Argentina, Brazil and Uruguay was in all cases lower than 0.8%, whereas the differences with the remaining Amblyomma species included in the analysis were always bigger than 6.8%. Thus, the taxonomic status of A. dubitatum along its distribution appears to be certain at the specific level.
      
      

    • Description of a new Argasid tick (Acari: Ixodida) from bat caves in Brazilian Amazon (Completo, 2010)

      NAVA, S. , VENZAL, J.M. , TERASSINI, F.A. , MANGOLD, A.J. , CAMARGO, L.M.A. , LABRUNA, M.B.
      Journal of Parasitology, v.: 96 6 , p.:1089 - 1101, 2010
      Palabras clave: Argasidae bats Nothoaspis amazoniensis Ixodida
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00223395
      E-ISSN: 19372345
      Nothoaspis amazoniensis n. sp. (Acari: Ixodida: Argasidae) is described from adult and immature ticks (nymph II, nymph I, larva) collected from bat caves in the Brazilian Amazon. Also, 16S rDNA sequences are provided. The diagnostic characters for adults are the presence of false shield or nothoaspis, an anteriorly projecting hood covering the capitulum, a medial extension of palpal article I (flaps), genital plate extending from coxa I to IV, absence of 2 setae on the internal margin of the flaps, a minute hypostome without denticles, presence of a central pore in the base of hypostome, and a reticulate surface pattern on the posterior half of the nothoaspis in males. The nymph II stage is characterized by a hood that is small in relation to the capitulum, short coxal setae, palpal flaps lacking setae on the internal margin, long hypostome, pointed with dentition 4/4 apically, and the anterior half of the body is covered by a cell-like configuration. Nymph I stage is characterized by a hood, small in relation to the capitulum, dorsum of the body covered by a cell-like configuration, venter integument covered by a cell-like configuration, and hypostome dentition 4/4 with apices that are ‘‘V’’-shaped. Diagnostic characters of the larvae are the number and size of dorsal setae, and the shape of scutum and hypostome. The new species appears to have a life cycle with a larva that feeds on bats, a non-feeding nymphal stage (nymph I), a feeding nymphal stage (nymph II), and adults that probably represent non-feeding stages.
      
      

    • A review of the systematics of the tick family Argasidae (Ixodida) (Completo, 2010)

      ESTRADA-PEÑA, A. , MANGOLD, A.J. , NAVA, S. , VENZAL, J.M. , LABRUNA, M.B. , GUGLIELMONE, A.A.
      Acarologia, v.: 50 3 , p.:317 - 333, 2010
      Palabras clave: Argasidae phylogenetic relationships morphology ecology cladistics 16S sequences
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 0044586X
      E-ISSN: 21077207
      DOI: 10.1051/acarologia/20101975
      The tick family Argasidae is a complex and diverse assemblage of about 190 species. Taxa within the Family Ixodidae had received considerable attention by contemporary researchers, but currently there is no consensus about the relevant morphological features for the determination of argasid species nor there is consensus on the appropriate genus for about 130 species. As a result, many species of Argasidae have not been yet adequately described. The generic and subgeneric arrangements are still a matter of discussion and currently there is no strict description of supraspecific systematics of this group. In this review, we introduce the competing proposals for the systematics of the family Argasidae, together with an overview of prominent morphological, ecological and behavioural features, which are of interest in a synthesis of the family. Special attention is paid to previous attempts to systematize the group based on cladistic analysis, outlining the possible pros and cons of such a method. We also present the largest phylogenetic tree of the family in terms of the number of species resolved by molecular biology based on 16S rDNA. This tree provides support for some of the previously proposed systematic arrangements based on morphology alone, and shows no basic differences with other previously published trees using either nuclear or mitochondrial, coding or non-coding genes. Our main conclusion is that we are still far from an accurate view of the main evolutionary lines of the family. There is thus an urgent need to obtain additional material to, first, explore the relative position of the different species in the phylogenetic arrangement of the family, and second, to capture adequate morphological features which could support a systematic key, necessary for any kind of faunistic or epidemiological studies.
      
      

    • Estudio de una colección de garrapatas (Acari: Ixodidae) de pecaríes (Artiodactyla: Tayassuidae) de Salta, Argentina. (Completo, 2010)

      MASTROPAOLO, M. , NAVA, S. , VENZAL, J.M. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      FAVE, v.: 9 1 , p.:27 - 31, 2010
      Palabras clave: AMBLYOMMA hospedadores Haemaphysalis Tayassuidae Rhipicephalus
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03253112
      Se estudiaron las garrapatas de pecaríes (Catagonus wagneri, Tayassu pecari, Tayassu tajacu) de Salta, Argentina, obtenidas en hallazgos ocasionales desde el 2007 a marzo de 2010. Amblyomma boeroi fue determinada exclusivamente sobre C. wagneri; una especie del grupo Amblyomma cajennense fue hallada sobre C. wagneri y T. pecari; Amblyomma neumanni fue observada parasitando C. wagneri y T. tajacu; Rhipicephalus microplus fue determinada sobre C. wagneri y Haemaphysalis juxtakochi sobre T. pecari. El registro de H. juxtakochi es el primero para este hospedador en la Argentina; igual condición corresponde al registro de la especie del grupo A. cajennense sobre C. wagneri, mientras que los registros de A. neumanni y R. microplus en C. wagneri son los primeros para esas especies y hospedador. Amblyomma parvum fue registrado previamente sobre pecaríes en el área de estudio por lo que no se incluye dicha garrapata en este trabajo.
      
      

    • Amblyomma boeroi n. sp. (Acari: Ixodidae), a parasite of the Chacoan peccary Catagonus wagneri (Rusconi) (Artiodactyla: Tayassuidae) in Argentina. (Completo, 2009)

      NAVA, S. , MANGOLD, A.J. , MASTROPAOLO, M. , VENZAL, J.M. , OSCHEROV, E.B. , GUGLIELMONE, A.A.
      Systematic Parasitology, v.: 73 p.:161 - 174, 2009
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01655752
      E-ISSN: 15735192
      All parasitic stages of Amblyomma boeroi n. sp. (Acari: Ixodidae) are described here from Catagonus wagneri (Rusconi) in Argentina. The diagnostic characters for the male are a combination of orbited eyes, a 2/2 dental formula, coxa IV considerably larger than coxae I–III and with a long, sickle-shaped, medially directed spur arising from its internal margin, a scutum which is light grey to very pale ivory in colour, and the absence of a postanal groove. The diagnostic characters for the females are a combination of orbited eyes, a central pair and two marginal pairs of short, coarse notal setae, a 2/2 dental formula, and the absence of a postanal groove. The nymph has short palpi and a 2/2 dental formula arranged in 6 rows, its eyes are convex and orbited, and it has no postanal groove. The dorsally rectangular basis capituli of the larva, its bulging eyes and slightly sinuous posterior scutal margin all serve to distinguish it from the larva of other species of the genus. The principal host for all parasitic stages is C. wagneri (Artiodactyla: Tayassuidae). Phylogenetically A. boeroi appears to represent an independent lineage within Amblyomma Koch, 1844.
      
      

    • Two new records of helminth parasites of domestic cat from Uruguay: Alaria alata (Goeze, 1782) (Digenea, Diplostomidae) and Lagochilascaris major Leiper, 1910 (Nematoda, Ascarididae) (Completo, 2009)

      CASTRO, O. , VENZAL, J.M. , FÉLIX, M.L.
      Veterinary Parasitology, v.: 160 p.:344 - 347, 2009
      Palabras clave: Uruguay Digenea Alaria alata Nematoda Lagochilascaris major Cat
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03044017
      Eight helminth taxa were recovered from the necropsy of four stray domestic cats from Colonia Miguelete, county of Colonia, Uruguay. Two of them are recorded for the first time for domestic cats in that country: Alaria alata (Goeze, 1782) from the small intestine (which is also the first trematode species found in domestic cat in Uruguay), and Lagochilascaris major Leiper, 1910 from the pharynx. The remaining helminth species found were Toxocara mystax (Zeder, 1800) and Spirometra sp. from the small intestine, Trichuris serrata (von Linstow, 1879) from the caecum, Eucoleus aerophilus (Creplin, 1839) from the trachea, and Pearsonema feliscati (Diesing, 1851) from the urinary bladder. Moreover, four female specimens of an unidentified Spiruroidea were collected from the stomach and small intestine of one host.
      
      

    • Bartonella rochalimae and Other Bartonella spp. in Fleas, Chile. (Completo, 2009)

      PÉREZ-MARTÍNEZ, L. , VENZAL, J.M. , GONZÁLEZ-ACUÑA, D. , PORTILLO, A. , BLANCO, J.R. , OTEO, J.A.
      Emerging Infectious Diseases, v.: 15 7 , p.:1150 - 1152, 2009
      Areas de conocimiento:
      Ciencias Médicas y de la Salud / Ciencias de la Salud / Enfermedades Infecciosas /
      Medio de divulgación: Papel
      ISSN: 10806040
      E-ISSN: 10806059
      
      

    • Carios fonsecai sp. nov. (Acari, Argasidae), a bat tick from the central-western region of Brazil (Completo, 2009)

      LABRUNA, M.B. , VENZAL, J.M.
      Acta Parasitologica, v.: 54 4 , p.:355 - 363, 2009
      Palabras clave: Argasidae Carios fonsecai sp.nov. bat Brazil
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 12302821
      E-ISSN: 18961851
      DOI: 10.2478/s11686-009-0051-1
      Males, females, and larvae of Carios fonsecai sp. nov. are described from free-living ticks collected in a cave at Bonito, state of Mato Grosso do Sul, Brazil. The presence of cheeks and legs with micromammillate cuticle makes adults of C. fonsecai morphologically related to a group of argasid species (mostly bat-associated) formerly classified into the subgenus Alectorobius, genus Ornithodoros. Examination of larvae indicates that C. fonsecai is clearly distinct from most of the previously described Carios species formerly classified into the subgenus Alectorobius, based primarily on its larger body size, dorsal setae number, dorsal plate shape, and hypostomal morphology. On the other hand, the larva of C. fonsecai is most similar to Carios peropteryx, and Carios peruvianus, from which differences in dorsal plate length and width, tarsal setae, and hypostome characteristics are useful for morphological differentiation. The mitochondrial 16S rDNA sequence of C. fonsecai showed to be closest (85–88% identity) to several corresponding sequences of different Carios species available in GenBank. Bats identified as Peropteryx macrotis and Desmodus rotundus were found infested by C. fonsecai larvae in the same cave where the type series was collected. C. fonsecai showed to be aggressive to humans in the laboratory.
      
      

    • Detection of Alpha and Gamma-Proteobacteria in Amblyomma triste Koch, 1844 (Acari: Ixodidae) from Uruguay. (Completo, 2008)

      VENZAL, J.M. , ESTRADA-PEÑA, A. , PORTILLO, A. , MANGOLD, A.J. , CASTRO, O. , DE SOUZA, C.G. , FÉLIX, M.L. , PÉREZ-MARTÍNEZ, L. , SANTIBÁÑEZ, S. , OTEO, J.A.
      Experimental and Applied Acarology, v.: 44 1 , p.:49 - 56, 2008
      Palabras clave: Uruguay Amblyomma triste Alpha-Proteobacteria Gamma-Proteobacteria Francisella-like Endosymbiont
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      Amblyomma triste is the most prevalent tick species reported in human tick bites in Uruguay and has been found to be infected with Rickettsia parkeri, but no other microorganisms have been reported from this tick. A sample of 254 adults of A. triste was collected by flagging on vegetation in suburban areas in southern Uruguay. Pools of five ticks were assembled and a screening for the DNA from the resulting 51 pools was realized by PCR assays using primers for amplifying a fragment of 16S rRNA gene for members of Anaplasmataceae. Seventeen pools were positive (33%) and the sequenciation of the gene fragment amplified revealed the presence of a putative new Alpha-Proteobacterium (denominated Atri-uru). The phylogenetic analysis showed that this microorganism is closely related to the symbiont of I. ricinus denominated ‘Candidatus Midichloria mitochondrii’ and other associated organisms. This rickettsial symbiont of ticks is included in a recent new clade proposed for the Alpha subclass of the Proteobacteria. The discovery of this bacterium in A. triste is the Wrst evidence of this group of Rickettsiales detected in the Genus Amblyomma, and the Wrst record in South America. Also, in two of 17 positive samples a Gamma-Proteobacterium related to Francisella-like organisms was detected.
      
      

    • Overview: Ticks as vectors of pathogens that cause disease in humans and animals. (Completo, 2008)

      DE LA FUENTE, J. , ESTRADA-PEÑA, A. , VENZAL, J.M. , KOCAN, K.M. , SONENSHINE, D.E.
      Frontiers in Bioscience, v.: 13 p.:6938 - 6946, 2008
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 10939946
      E-ISSN: 10943935
      Ticks (Acari: Ixodidae) transmit a wide variety of pathogens to vertebrates including viruses, bacteria, protozoa and helminthes. Tick-borne pathogens are believed to be responsible for more than 100,000 cases of illness in humans throughout the world. Ticks are considered to be second worldwide to mosquitoes as vectors of human diseases, but they are the most important vectors of disease-causing pathogens in domestic and wild animals. Infection and development of pathogens in both tick and vertebrate hosts are mediated by molecular mechanisms at the tick-pathogen interface. These mechanisms, involving traits of both ticks and pathogens, include the evolution of common and species-specific characteristics. The molecular characterization of the tick-pathogen interface is rapidly advancing and providing new avenues for the development of novel control strategies for both tick infestations and their associated pathogens.
      
      

    • Amblyomma triste Koch, 1844 (Acari: Ixodidae): Hosts and seasonality of the vector of Rickettsia parkeri in Uruguay. (Completo, 2008)Trabajo relevante

      VENZAL, J.M. , ESTRADA-PEÑA, A. , CASTRO, O. , DE SOUZA, C.G. , FÉLIX, M.L. , NAVA, S. , GUGLIELMONE, A.A.
      Veterinary Parasitology, v.: 155 p.:104 - 109, 2008
      Palabras clave: Uruguay Amblyomma triste Hosts Seasonality Rickettsia parkeri
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03044017
      Amblyomma triste Koch, 1844, the tick species most frequently involved in human bites in Uruguay, has been implicated as a vector of human rickettsiosis. Seasonal abundance of adult A. triste was examined by standard flagging at three sites where human tick bites and cases of the disease have been reported. Adult tick activity occurred from August to February (end of winter to mid summer in the southern hemisphere) with a peak in spring. Activity declined in step with decreasing temperatures and photoperiod during winter. This period of activity coincides with seasonal outbreaks of human rickettsiosis in the region. In a small mammal survey, the Sigmodontinae rodents Scapteromys tumidus (Waterhouse, 1837) and Oxymycterus nasutus (Waterhouse, 1837) and the small marsupial Monodelphis dimidiata (Wagner, 1847) were the main hosts for immature A. triste. Immature ticks were observed on hosts in November, well within the period of peak adult abundance. In stored collections, immature ticks were most often collected from January to March. These data suggest that one generation might be completed in 1 year. The main animal host for adult A. triste at our study sites was the domestic dog. Humans were afflicted by the tick in rural and suburban settlements where other host animals are scarce or extinct and where dogs are common.
      
      

    • Hosts and distribution of Ixodes longiscutatus Boero, 1944 (Acari: Ixodidae). (Completo, 2008)

      VENZAL, J.M. , NAVA, S. , BELDOMÉNICO, P.M. , BARROS-BATTESTI, D.M. , ESTRADA-PEÑA, A. , GUGLIELMONE, A.A.
      Systematic and Applied Acarology, v.: 13 p.:102 - 108, 2008
      Palabras clave: Uruguay Ixodidae Argentina Hosts Ixodes longiscutatus
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 13621971
      E-ISSN: 20566069
      Specimens of Ixodes longiscutatus Boero, 1944 (Ixodidae) were collected in Uruguay and, to a lesser extent, Argentina from 1998 to 2004. Specimen data and literature records were used to compile a comprehensive list of hosts and to define the ecological parameters of this tick species, which is currently known from a total of only 6 females, 37 nymphs and 94 larvae. The male remains undescribed. Bona fide records of females were from cattle, horse, Cavia tschudii Fitzinger, 1857 and Cavia sp. (Rodentia: Caviidae). Most nymphs were collected on rodents of the family Cricetidae, especially Oxymycterus nasutus (Waterhouse, 1837) and Scapteromys tumidus (Waterhouse, 1837), but also C. tschudii. Again, most larvae were found on cricetid rodents, namely O. nasutus, S. tumidus, Akodon lutescens Allen, 1901, Oligoryzomys flavescens (Waterhouse, 1837) and Necromys obscurus (Waterhouse, 1837), and on Caviidae (Cavia aperea Erxleben, 1777 and C. tschudii). Both larvae and nymphs were found on hosts during all seasons of the year, with a higher prevalence in summer. Two allopatric populations of I. longiscutatus appear to exist, one in Uruguay and neighboring provinces of Argentina (Buenos Aires and Entre Ríos), the other in northwestern Argentina (Salta Province); DNA analysis may confirm their conspecificity. Scanning electron photomicrographs of the capituli of larvae and nymphs, as well as the posterior setae of the nymphal idiosoma, are provided.
      
      

    • Observations on Antricola Ticks: Small Nymphs Feed on Mammalian Hosts and Have a Salivary Gland Structure Similar to Ixodid Ticks. (Completo, 2008)

      ESTRADA-PEÑA, A. , VENZAL, J.M. , KOCAN, K.M. , TRAMUTA, C. , TOMASSONE, L. , DE LA FUENTE, J. , LABRUNA, M.B.
      Journal of Parasitology, v.: 94 4 , p.:953 - 955, 2008
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00223395
      E-ISSN: 19372345
      Ticks use bloodmeals as a source of nutrients and energy to molt and survive until the next meal and to oviposit, in the case of females. However, only the larvae of some tick species are known to feed upon bats; females are obligatorily autogenous, and nymphal stages are believed to not feed. We investigated the presence of blood in a natural population of nymphal Antricola delacruzi ticks collected from bat guano; their ability to feed upon laboratory hosts; and the microscopic structure of both salivary glands and gut. DNA amplification of gut contents of freshly collected material was positive for a mammal in 4 of 11 first instar nymphs, but we were unsuccesful in the amplification of host bloodmeal DNA from late instar nymphs. All early nymphal stages (n _ 10) fed on rabbits, and host DNA was detected and sequenced from gut contents. However, all the large nymphs (n _ 10) rejected feeding, and host DNA remained undetected in these ticks. All stages of A. delacruzi have salivary glands similar in morphology to the ixodid agranular Type I salivary gland acini and to granular Type II or Type B acini. All stages of A. delacruzi had a similar gut structure, consisting of digestive cells in the basal portion that contained hematin granules. Neither regenerative nor secretory cell traces were observed in the sections of gut.
      
      

    • Rickettsia felis in Ctenocephalides felis from Argentina. (Completo, 2008)

      PÉREZ-MARTÍNEZ, L. , NAVA, S. , VENZAL, J.M. , PORTILLO, A. , SANTIBÁÑEZ, S. , OTEO, J.A.
      Vector-Borne and Zoonotic Diseases, v.: 8 4 , p.:465 - 466, 2008
      Palabras clave: Argentina Rickettsia felis Ctenocephalides felis
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 15303667
      E-ISSN: 15577759
      This article reports on the detection of Rickettsia felis in Argentina in Ctenocephalides felis fleas collected from a dog and analyzed by polymerase chain reaction (PCR) amplification and DNA sequencing.
      
      

    • The Ornithodoros (Alectorobius) talaje species group (Acari: Ixodida: Argasidae): Description of Ornithodoros (Alectorobius) rioplatensis n. sp. from southern South America. (Completo, 2008)

      VENZAL, J.M. , ESTRADA-PEÑA, A. , MANGOLD, A.J. , GONZÁLEZ-ACUÑA, D. , GUGLIELMONE, A.A.
      Journal of Medical Entomology, v.: 45 5 , p.:832 - 840, 2008
      Palabras clave: distribution Ornithodoros (Alectorobius) talaje species group Ornithodoros (Alectorobius) rioplatensis n. sp. mitochondrial 16S rDNA sequence
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00222585
      E-ISSN: 19382928
      A new species of argasid tick, Ornithodoros (Alectorobius) rioplatensis Venzal, Estrada-Peña, & Mangold, is described from larval and adult specimens collected in Uruguay. Further specimens from Argentina, Paraguay, and Chile are ascribed to the new species. Key distinguishing characters of larvae of the species are the number of pairs of dorsal setae (19-20 but typically 20 in O. rioplatensis, compared with 16-17 but typically 17 in O. puertoricensis and O. talaje), and the length of the hypostome, which is clearly longer in O. rioplatensis than in O. talaje. Morphological details of the adults of these species are inadequate for taxonomic purposes. The genetic distance between O. puertoricensis and O. rioplatensis, based on 16S rDNA sequencing, is ~ 12.7%. Variability in some morphological features, using multivariate morphometric discrimination to assess congruence between populations, was examined among larval specimens of O. talaje and O. puertoricensis collected from the United States, and Central and South America. Larval specimens ofO. talaje from Guatemala (near the type locality of the species) were morphologically different from Mexican and southern U.S. populations originally described as O. talaje. In the absence of DNA data for these populations, and because of inadequate statistical discrimination among body characters, we prefer to refer to the Mexican and U.S. material as O. nr. talaje until further analysis of population variability is conducted using an adequate sample size.
      
      

    • Toxoplasma gondii: Cross-immunity against the enteric cycle. (Completo, 2007)

      FREYRE, A. , FALCÓN, D. , MÉNDEZ, J. , GASTELL, T. , VENZAL, J.M.
      Experimental Parasitology, v.: 115 p.:48 - 52, 2007
      Palabras clave: Toxoplasma Cross-immunity Enteric cycle
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00144894
      E-ISSN: 10902449
      Eight of nine cats inoculated with strain ME-49 and challenged with three diVerent strains of Toxoplasma were immune to oocyst shedding, as ascertained with bioassays of their feces. In a second experiment, only toxoplasma asexual stages were seen in H&E stained gut sections of cats treated with suppressive doses of sulfamerazine and pyrimethamine starting 2 days after oral inoculation with cysts of the strain ME-49 and killed 6 days later. In a third experiment, four cats were similarly inoculated and treated for 20 days. Six weeks later, the cats received an oral homologous challenge with cysts, and none shed toxoplasma oocysts. An acceptable level of cross-protection was achieved with strain ME-49, and therefore, it can be used as a candidate strain from which antigens could be tested for enteric protection.
      
      

    • Using invaded range data to model the climate suitability for Amblyomma variegatum (Acari: Ixodidae) in the New World. (Completo, 2007)

      ESTRADA-PEÑA, A. , PEGRAM, R.G. , BARRÉ, N. , VENZAL, J.M.
      Experimental and Applied Acarology, v.: 41 3 , p.:203 - 214, 2007
      Palabras clave: habitat suitability modelling Amblyomma variegatum Spread New World
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      Climate matching models are in increasing use to predict distributions of living organisms, using records of the known distribution of a species to map its expected range of habitat suitability. Here, we modelled the known distribution of the tick Amblyomma variegatum in Africa as a preliminary step to delineate the most probable range of climatically suitable habitat for the tick in the New World. We used two presence-only methods (one based in the Gower distance, the other based on the Maximum Entropy principle) to model the distribution range in Africa. The Maximum Entropy method is highly dependent of the realized niche of the tick, and has serious constraints in the case of lack of adequate description of the actual range of the tick. The Gower distance, however, can evaluate the fundamental niche of the tick and produced better results with the same set of distribution data. Several populations of A. variegatum were recognized in Africa on the basis of statistically different ecological attributes. The separate modelling of the climate envelope for these populations provided a better Wt in the delineation of habitat suitability with both methods in Africa but produced high rates of false negatives when applied to the Caribbean. The best modelling strategy for the tick in the New World (according to the rate of false negatives) is the use of Gower distance together with the known distribution of the tick in the Caribbean. The potential spread area of the tick includes all the Caribbean, large areas of Colombia and Venezuela, parts of Brazil, most of the Mesoamerican corridor and Mexico as well as the Peninsula of Florida. We do not consider further if the invading strain either still retains the full ecological plasticity of the original populations in Africa, or has already adapted to the invaded area, resulting in a more restricted ability to expand. Both possibilities have deep impact in our analyses, as the tick could find a larger zone for spreading into the New World.
      
      

    • The Ornithodoros hasei (Schulze, 1935) (Acari: Argasidae) species group in Argentina. (Completo, 2007)

      NAVA, S. , VENZAL, J.M. , DÍAZ, M.M , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Systematic and Applied Acarology, v.: 12 p.:27 - 30, 2007
      Palabras clave: Argasidae Argentina Ornithodoros hasei bats
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 13621971
      E-ISSN: 20566069
      Six larvae of the genus Ornithodoros were collected in Rio Lavayén, approximately 1km north of Santa Rita, San Pedro Department (24º 28´S, 64º 48´W), Jujuy Province, Argentina, on three species of bats: Molossops temminckii (Burmeister, 1854) (Molossidae), Myotis albescens (E. Geoffroy, 1806) (Vespertilionidae) and Histiotus laephotis Thomas, 1916 (Vespertilionidae). All six larvae were identified as belonging to the Ornithodoros hasei species group, whose members are common parasites of bats throughout the Neotropical Zoogeographic Region. This is the first record of the O. hasei species group in Argentina, and M. temminckii and H. laephotis are new hosts for this tick. Studies of the morphology, genetics and ecology of O. hasei across its vast range will be necessary in order to determine whether sibling species are present.
      
      

    • A GIS framework for the assessment of tick impact on human health in a changing climate. (Completo, 2007)

      ESTRADA-PEÑA, A. , VENZAL, J.M.
      Geospatial health, v.: 2 p.:157 - 168, 2007
      Palabras clave: tick-borne disease tick impact society vulnerability human health climate change
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 18271987
      E-ISSN: 19707096
      A framework to evaluate the impact of ticks on human health under various scenarios of climate change is proposed. The purpose is not to provide a comprehensive plan (e.g. the economic impact of ticks on human society is not included), instead we wish to describe a series of indices that would be helpful by obtaining homogeneous comparisons of impact and vulnerability exerted by ticks in different regions, countries or continents, using normalized sets of population, vegetation, climate and physical attributes of the territory. Three tick species, i.e. Dermacentor marginatus, Rhipicephalus turanicus and Hyalomma marginatum, have been traced over the territory of Spain to further explain the computation of these indices. The discussion is based on tick habitat suitability, used as a measure of the abiotic (climate) fitness of the habitat for the species in question, and the sensitivity of each tick species to the rate of change of habitat suitability with respect to climate change. The impact is the rate of change in habitat suitability weighted with a fuzzy logic function evaluating the total number of people in an area, percent of rural population and accessibility of the geographical divisions (expressed as hexagons with a 10 km radius) used in the study. The different climate scenarios evaluated in relation to ticks show that the north-western part of Spain would suffer the greatest impact in case the mean temperature would increase, while the Mediterranean region would suffer the highest impact if temperatures decreased. Vulnerability, based on the sanitary structure of the territory and on the impact on human activities due to the change in tick distribution and abundance, is proposed as a measure of adaptation of society to these climate scenarios. The cost is evaluated as a function of land use and tick habitat suitability in a buffer zone surrounding each geographic division. All indices proposed have been obtained by search of common and/or publicly available data sets.
      
      

    • Effects produced by the feeding of larvae of Ornithodoros aff. puertoricensis Fox, 1947 (Acari: Argasidae) on laboratory mice. (Completo, 2007)

      VENZAL, J.M. , ESTRADA-PEÑA, A. , FERNÁNDEZ DE LUCO, D.
      Experimental and Applied Acarology, v.: 42 3 , p.:217 - 223, 2007
      Palabras clave: Ornithodoros aff. puertoricensis Toxicosis Larvae Laboratory mice
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      The clinical picture produced by the feeding of larvae of Ornithodoros aff. puertoricensis on laboratory mice, was studied using different larval infestation protocols that included 30, 40 or 50 larvae per mouse and control uninfested groups. Clinical effects appeared around 72 h of larval feeding, having a Wrst stage characterized by hyperaemia in both nasal and ocular mucosa, followed by respiratory symptoms (96–120 h) and nervous incoordination (120–144 h). No one mouse evidenced paralysis, and nervous symptoms were never observed in animals infested with only 30 larvae. High mortality (commonly up to 70%) was observed in mice with respiratory symptoms, while 100% of animals in the nervous phase died between 168 and 192 h after the beginning of larval feeding. When some infested mice were treated with a solution of Amitraz the larvae were killed and reversion of symptoms was observed. These effects are ascribed to the presence of a toxin in the saliva of the feeding larvae.
      
      

    • Study on seasonal activity in dogs and ehrlichial infection in Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) from southern Uruguay. (Completo, 2007)

      VENZAL, J.M. , ESTRADA-PEÑA, A. , CASTRO, O. , DE SOUZA, C.G. , PORTILLO, A. , OTEO, J.A.
      Parasitología latinoamericana, v.: 62 1-2 , p.:23 - 26, 2007
      Palabras clave: Uruguay Rhipicephalus sanguineus seasonal activity dogs absence of ehrlichial DNA
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      E-ISSN: 07177712
      The seasonal activity on dogs of Rhipicephalus sanguineus in southern Uruguay has been studied from samples collected in veterinary clinics of the country. From a total of 744 specimens, adults (92%) were active on dogs between August and March (peaking in October) being absent between April and July. Immatures represent more than 80% of captures on dogs in summer. This pattern is well correlated with the climate records in the zone. The search for ehrlichial DNA from tick-derived extracts (36 pools from 180 ticks) was negative in all the cases. While R. sanguineus has been incriminated as vector of these pathogens, additional studies are required to understand the role of this tick in the transmission of ehrlichial organisms.
      
      

    • Tick collections from the Peruvian Amazon, with new host records for species of Ixodes Latreille, 1795 (Acari: Ixodidae) and Ornithodoros Koch, 1844 (Acari: Argasidae). (Completo, 2007)

      DÍAZ, M.M , NAVA, S. , VENZAL, J.M. , SÁNCHEZ, N. , GUGLIELMONE, A.A.
      Systematic and Applied Acarology, v.: 12 p.:127 - 133, 2007
      Palabras clave: Ixodidae AMBLYOMMA Argasidae Ixodes Ornithodoros Peruvian Amazon
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 13621971
      E-ISSN: 20566069
      Tick-infested bats, rodents and marsupials were captured along the Iquitos-Nauta highway, Loreto Department, in the Peruvian Amazon. The only ticks found on Chiroptera were members of the Ornithodoros hasei (Schulze, 1935) species group, collected from Noctilio albiventris Desmarest, 1818, Rhinophylla pumilio Peters, 1865 and Myotis albescens (É. Geoffroy, 1806). Rhinophylla pumilio is a new host and M. albescens was confirmed as a host for the O. hasei group. Among Rodentia, Oecomys bicolor (Tomes, 1860), Oligoryzomys microtis (Allen, 1916), Hylaeamys perenensis (Allen, 1901) and Hylaeamys yunganus (Thomas, 1902) were infested with larvae (O. bicolor) or nymphs (other three rodents) of Ixodes luciae Sénevet, 1940; all are new hosts for I. luciae. Tick infestation was more diverse among the Didelphimorphia, where a nymph and larva of Amblyomma sp. Were collected on Philander opossum (Linnaeus, 1758), a nymph of Amblyomma sp. on Caluromys lanatus (Olfers, 1818), and a larva of Ixodes pararicinus Keirans & Clifford, 1985 on Monodelphis adusta (Thomas, 1897). Monodelphis adusta is a new host for I. pararicinus. Caluromys lanatus, Marmosops sp., Metachirus nudicaudatus (Desmarest, 1817), Micoureus sp., Philander andersoni (Osgood, 1913) and P. opossum were parasitized with larvae (Micoureus sp.) or adults (all other didelphids) of I. luciae. With the exception of P. opossum, all these marsupials are new hosts for I. luciae.
      
      

    • Climate Niches of Tick Species in the Mediterranean Region: Modeling of Occurrence Data, Distributional Constraints, and Impact of Climate Change. (Completo, 2007)

      ESTRADA-PEÑA, A. , VENZAL, J.M.
      Journal of Medical Entomology, v.: 44 6 , p.:1130 - 1138, 2007
      Palabras clave: Ticks Mediterranean region climate niche sensitivity climate change
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00222585
      E-ISSN: 19382928
      In this study, we used ecological niche factor analysis (ENFA) and principal components analysis (PCA) of climate variables to deÞne the climate niches and areas of potential colonization of six species of ticks in the Mediterranean region: Dermacentor marginatus Sulzer, Rhipicephalus bursa Canestrini & Fanzago, Rhipicephalus turanicus Pomerantsev, Matikashvili & Lototsky, Hyalomma marginatum Koch, Hyalomma excavatum Koch, and Boophilus annulatus (Say). ENFA generated distribution models that varied in accuracy from high to very high (area under the curve [AUC] _ 0.87Ð 0.97), with the lowest AUC obtained for B. annulatus. PCA provided an adequate separation of the climate niches of different species in the reduced space of the variables. Climate scenarios and factorial consensus analysis were used to evaluate the geographic impact of climate change (as turnover in habitat suitability) on the niches of the ticks and net variations in habitat availability. The scenario that was most compatible with estimates of future climate in the Mediterranean region (increase in temperature and decrease in rainfall) was predicted to produce a sharp increase in the extent of suitable habitat for R. bursa, R. turanicus, and H. marginatum. This scenario would result in a northward expansion of suitable habitat areas for these three species. The highest impact (highest species turnover) would be recorded at the margin of the current distribution range of the three species.Asensitivity analysis of the ecological response of the ticks to the climate change scenarios showed that the response is statistically different in different regions of the PCA-derived niche. These results outline the need to further investigate the potential of bioclimate models to obtain accurate estimations of tick species turnover under conditions of climate change over wide areas.
      
      

    • Piojos (Phthiraptera: Amblycera, Ischnocera) registrados en aves marinas y costeras en Uruguay. (Completo, 2007)

      VENZAL, J.M. , CASTRO, O. , KATZ, H. , FÉLIX, M.L. , MORGADES, D. , PALMA, R.L.
      Gayana, v.: 71 2 , p.:195 - 199, 2007
      Palabras clave: Uruguay Phthiraptera Amblycera Ischnocera lice marine and coastal birds
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 0717652X
      E-ISSN: 07176538
      In this study, 17 lice species were collected and identified from marine and coastal birds from the Atlantic Ocean coast, rivers and lagoons of Uruguay. Among them, 16 species (one at generic level only) are recorded for the first time for this country.
      
      

    • Garrapatas exóticas: hallazgo de Amblyomma latum Koch, 1844 (Acari: Ixodidae) parasitando Python regius (Serpentes: Boidae) en Uruguay (Completo, 2007)

      VENZAL, J.M. , NAVA, S. , GUGLIELMONE, A.A.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 42 168 , p.:15 - 17, 2007
      Palabras clave: Uruguay Amblyomma latum Python regius garrapatas exóticas
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03764362
      E-ISSN: 16884809
      La introducción de parásitos vectores a través de animales exóticos adquiridos como mascotas puede generar riesgos sanitarios. El ingreso de garrapatas que usan como hospedadores reptiles exóticos ha sido reportado en varios países. Mediante el examen de un ejemplar de Python regius en el departamento de Montevideo, Uruguay, fue hallada una garrapata hembra determinada como Amblyomma latum. Esta especie ha sido recientemente registrada en pitones importadas en Argentina y Chile. El hallazgo de A. latum en forma casi simultánea en estos tres países del cono sur, indica un ingreso poco controlado de los reptiles y sus garrapatas, y supone la posibilidad que A. latum u otras especies puedan establecerse y diseminarse en la naturaleza o criaderos de reptiles. Sería conveniente extremar las medidas de control sanitario sobre estos animales para impedir que ello ocurra.
      
      

    • The tick Ixodes ricinus: distribution and climate preferences in the western Palaearctic. (Completo, 2006)

      ESTRADA-PEÑA, A. , VENZAL, J.M. , SÁNCHEZ ACEDO, C.
      Medical and Veterinary Entomology, v.: 20 2 , p.:189 - 197, 2006
      Palabras clave: Ixodes ricinus clades habitat suitability modelling
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 0269283X
      E-ISSN: 13652915
      In this study, multivariate spatial clustering on monthly normalized difference vegetation index (NDVI) maps is used to classify ecological regions over the western Palaearctic. This classification is then used to delineate the distribution and climate preferences of populations (clades) of the tick Ixodes ricinus L. (Acari: Ixodidae) from a geographically extensive dataset of tick records and a gridded 2.5-km resolution climate dataset. Using monthly layers of the NDVI, regions of similar ecological attributes were defined and nine populations with significant differences in critical climate parameters ( P < 0.005) were detected. Grouping of tick records according to other categories, such as political divisions, a 4° × 4° grid overlying the study area, or the CORINE ) and USGS ) vegetation classification schemes did not provided significantly separated populations ( P = 0.094 – 0.304). Factor analysis and hierarchical tree clustering provided an ecological overview of these tick clades: two Mediterranean and one Scandinavian (western) clades are clearly separated from a node that includes clades of different parts of central Europe and the British Isles, with contrasting affinities between the different clades. The capture records of these ecologically separated clades produce a clear bias when bioclimate envelope modelling is applied to the mapping of habitat suitability for the tick in the western Palaearctic. The best-performing methods (Cohen ’ s kappa = 0.834 – 0.912) use partial models developed with data from each ecoregion, which are then overlapped over the region of study. It is concluded that the use of ecologically derived ecoregions is an objective step in assessing the presence of ecologically different clades, and provides a guide in the development of data partitioning for habitat suitability modelling.
      
      

    • Changes in climate and habitat suitability for the cattle tick Boophilus microplus in its southern Neotropical distribution range. (Completo, 2006)

      ESTRADA-PEÑA, A. , CORSON, M. , VENZAL, J.M. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Journal of Vector Ecology, v.: 31 1 , p.:158 - 167, 2006
      Palabras clave: Argentina Boophilus microplus climate scenarios habitat suitability models
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      E-ISSN: 10811710
      We addressed the possible effects of several climate scenarios on habitat suitability (HS) for the cattle tick Boophilus microplus and the probability of producing permanent populations from introduced females of that tick in central parts of Argentina, using both a correlative model (derived from climate predictors) and a mechanistic (life cycle) model. There was high correlation (R2 = 0.866) between HS-derived and life cycle outputs for HS values higher than 0.52, suggesting that HS is a good estimator of the life cycle of the tick above a critical threshold of HS values. Scenarios with increased temperatures increased suitable habitats for the tick in southern parts of the study region, extending below parallel 34º S, but suitable habitats remained limited in the west. A concurrent increase in rainfall produced a further increase of HS in these areas. Results from the life cycle model suggest that in areas of suitable habitat, permanent cattle tick populations are most probable if engorged females are introduced during mid-summer.
      
      

    • The phylogenetic position of Ixodes stilesi Neumann, 1911 (Acari: Ixodidae): morphological and preliminary molecular evidences from 16S rDNA sequences. (Completo, 2006)

      GUGLIELMONE, A.A. , VENZAL, J.M. , GONZÁLEZ-ACUÑA, D. , NAVA, S. , HINOJOSA, A , MANGOLD, A.J.
      Systematic Parasitology, v.: 65 1 , p.:1 - 11, 2006
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01655752
      E-ISSN: 15735192
      The female of Ixodes stilesi Neumann, 1911 (Acari Ixodidae) is redescribed and the male and nymph are described from specimens collected from Pudu puda (Molina) (Artiodactyla: Cervidae) in Chile. Both sexes of I. stilesi have characteristics of the subgenera Ixodes Latreille, 1795 and Ixodiopsis Filippova, 1957. The females of I. stilesi are peculiar in having the combination of the sinuous scutum outline, rounded porose areas with distinct borders separated by the width of one area, slender and long palpi, and two subequal spurs on coxa I. The male is unique in having a combination of a posteriorly wrinkled marginal folder, a basis capituli longer than wide, a non-crenulate hypostome toothed portion, two spurs on coxa II to IV and the presence of a pseudoscutum. The nymph of I. stilesi has blunt anterior and posterior processes on palpal article I (characteristics of the subgenus Ixodiopsis and some Pholeoixodes Schulze, 1942) and a wing-shaped basis capituli with a prominent triangular cornua. Phylogenetic analyses based on 16S mitochondrial rDNA sequences of 12 Neotropical and two Australian Ixodes species, plus three argasids, were carried out to clarify the position of I. stilesi. The results of phylogenetic analyses and morphological characters indicate a close relationships between I. stilesi and two other Neotropical species of uncertain subgeneric status, I. neuquenensis Ringuelet, 1947 and I. sigelos Keirans, Clifford & Corwin, 1976.
      
      

    • Larval feeding performance of two Neotropical Ornithodoros ticks (Acari: Argasidae) on reptiles. (Completo, 2006)

      VENZAL, J.M. , ESTRADA-PEÑA, A.
      Experimental and Applied Acarology, v.: 39 p.:315 - 320, 2006
      Palabras clave: Argasidae ticks Ornithodoros puertoricensis Ornithodoros rostratus Reptiles Larval feeding performance
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      This paper determines the feeding performance of the larvae of two Neotropical soft tick species namely Ornithodoros rostratus Aragão, 1911 and O. puertoricensis Fox, 1947 on reptiles (Gekkonidae) using rabbits, mice and guinea pigs to provide comparisons with feeding features on mammals. O. puertoricensis produced a larval feeding rate of 63% on reptiles, while that of O. rostratus was only 20%. But the final success (attaching + feeding) was similar, 12.4% for O. puertoricensis and 10.4% for O. rostratus. The feeding time was also very different for both species. In O. puertoricensis, detachment begins at 16th day and lasts until day 27. In O. rostratus detachment begins at 1.5 h and lasts until day 10. These values of feeding on reptiles are different from those obtained on mammals (average 5.6 days for O. puertoricensis and 2.9 for O. rostratus).
      
      

    • La presencia de Ixodes luciae en el noroeste argentino y nuevos huéspedes para Ixodes pararicinus y algunas especies de Amblyomma (Acari: Ixodidae). (Completo, 2006)

      AUTINO, A.G. , NAVA, S. , VENZAL, J.M. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Revista de la Sociedad Entomológica Argentina, v.: 65 1-2 , p.:27 - 32, 2006
      Palabras clave: AMBLYOMMA Carnivora Didelphimorphia Ixodes Rodentia
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      E-ISSN: 03735680
      The analysis of a tick collection of mammals from northwestern Argentina deposited in the Annexes (ACML) of Colección Mamíferos Lillo (CML) National University of Tucumán, amplified the distribution of Ixodes luciae Sénevet, to Salta and Tucumán provinces and increased the host range to the sigmodontinae rodent Calomys callosus (Rengger) and the Didelphidae Micoureus constantiae (Thomas), Thylamys cinderella Thomas and Thylamys venustus (Thomas). Calomys callosus and Oligoryzomys destructor (Tschudi) were registered as new hosts of Ixodes pararicinus Keirans & Clifford, and the Carnivora Lycalopex gymnocercus (Fischer) and Oncifelis geoffroyi (d´Orbigny & Gervais) as new hosts of Amblyomma neumanni Ribaga, and Amblyomma parvum Aragão, respectively. Lutreolina crassicaudata (Desmarest) (Didelphidae) was also found infested with I. luciae while Amblyomma tigrinum Koch was detected on L. gymnocercus. Nymphs of Amblyomma sp. were found on Akodon Meyen. (Sigmodontinae).
      
      

    • Notes on New World Persicargas Ticks (Acari: Argasidae) with Description of Female Argas (P.) keiransi. (Completo, 2006)

      ESTRADA-PEÑA, A. , VENZAL, J.M. , GONZÁLEZ-ACUÑA, D. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Journal of Medical Entomology, v.: 43 5 , p.:801 - 809, 2006
      Palabras clave: Argas (Persicargas) keiransi 16S sequence keys New World Persicargas
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00222585
      E-ISSN: 19382928
      The female of Argas (Persicargas) keiransi Estrada-Peña, Venzal & González-Acuña (Acari: Argasidae) is described from specimens collected in the type locality in Chile. The female has a unique combination of characters in the dorsal cuticle and in the number and pattern of ventrolateral setae in the basis capituli. Additional features on tarsus I chaetotaxy and dorsal plate are provided for the larva of the species, as observed on flat specimens derived from engorged females collected in the type locality. Keys for the larvae of the New World Persicargas are provided as well as illustrations for prominent characters in hypostome and posterolateral setae. The mitochondrial 16S rDNA sequence of A. keiransi has been obtained, and an analysis of the phylogenetic relationships with other ticks is included. Phylogenetic analysis provides support for a Persicargas grouping of species, separated from species in Argas.
      
      

    • Fleas of Small Mammals in Uruguay, with New Host and Distribution Records. (Completo, 2006)

      LARESCHI, M. , VENZAL, J.M. , ARZUA, M. , GONZÁLEZ, E.M.
      Comparative Parasitology, v.: 73 2 , p.:263 - 268, 2006
      Palabras clave: Uruguay fleas- Siphonaptera Polygenis species Craneopsylla minerva minerva Sternopsylla distincta
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 15252647
      E-ISSN: 19382952
      Fleas are ectoparasitic insects of many birds and mammals. More than 200 species and subspecies of fleas have been recorded from South America; however, they have been poorly studied in Uruguay. In the present study, Polygenis (Neopolygenis) atopus, Polygenis (Neopolygenis) massoiai, Polygenis (Polygenis) axius axius, Craneopsylla minerva minerva, and Sternopsylla distincta are reported for the first time in Uruguay. These fleas parasitize wild rodents, marsupials, and bats, and most of the ectoparasite–host associations are recorded for the first time. Results obtained are consistent with findings in nearby Argentinean and Brazilian localities.
      
      

    • Rickettsia parkeri in Uruguay. (Completo, 2006)

      PACHECO, R.C. , VENZAL, J.M. , RICHTZENHAIN, L.J. , LABRUNA, M.B.
      Emerging Infectious Diseases, v.: 12 11 , p.:1804 - 1805, 2006
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 10806040
      E-ISSN: 10806059
      
      

    • Prevalence of Rickettsia felis in Ctenocephalides felis and Ctenocephalides canis from Uruguay. (Completo, 2006)

      VENZAL, J.M. , PÉREZ-MARTÍNEZ, L. , FÉLIX, M.L. , PORTILLO, A. , BLANCO, J.R. , OTEO, J.A.
      Annals of the New York Academy of Sciences, v.: 1078 1 , p.:305 - 308, 2006
      Palabras clave: Uruguay Rickettsia felis Ctenocephalides felis Ctenocephalides canis
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00778923
      E-ISSN: 17496632
      Our aim was to determine the presence of Rickettsia spp. In 66 fleas from Uruguay. Rickettsial DNA was amplified using gltA and ompB PCR primers. Rickettsia spp. were found in 41% of the fleas (25 Ctenocephalides felis and 2 Ctenocephlides canis). Sequences resulted in the identification of Rickettsia felis and four genotypes closely related to this species (Rickettsia sp. TwKM03, California 2, Hf187, and RF2125). The presence of R. felis in fleas from Uruguay in was demonstrated. This is the second species of Rickettsia identified in Uruguay in the past 2 years using molecular approaches, and it is helping to clarify the etiology of rickettsial diseases in the region.
      
      

    • Efficacy of Several Anti-Tick Treatments to Prevent the Transmission of Rickettsia conorii under Natural Conditions. (Completo, 2006)

      ESTRADA-PEÑA, A. , VENZAL, J.M.
      Annals of the New York Academy of Sciences, v.: 1078 1 , p.:506 - 508, 2006
      Palabras clave: Rickettsia conorii anti-tick treatment protection
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00778923
      E-ISSN: 17496632
      The efficacy of several anti-tick treatments to prevent the transmission of Rickettsia was evaluated under natural conditions of tick pressure in a kennel. Only Amitraz (Preventic) provided total control on transmission (no dogs were infected), whereas with Frontline, Advantix, or Scalibor, the rate of infection varied among the dogs.
      
      

    • First Report of Mites (Gamasida: Laelapidae) Parasitic on Wild Rodents in Uruguay, with New Host Records. (Completo, 2006)

      LARESCHI, M. , GETTINGER, D. , VENZAL, J.M. , ARZUA, M. , NIERI-BASTOS, F.A. , BARROS-BATTESTI, D.M. , GONZÁLEZ, E.M.
      Neotropical Entomology, v.: 35 5 , p.:596 - 601, 2006
      Palabras clave: Acari ectoparasite Haemogamasinae
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 1519566X
      E-ISSN: 16788052
      Information is presented for the first time on laelapid mites associated with wild rodents in Uruguay. Specimens of the following species were identified: Laelapinae: Androlaelaps fahrenholzi (Berlese), Androlaelaps rotundus (Fonseca), Gigantolaelaps wolffsohni (Oudemans), Laelaps paulistanensis (Fonseca), Laelaps manguinhosi (Fonseca) and Mysolaelaps microspinosus Fonseca; Haemogamasinae: Eulaelaps stabularis (Koch). Most of the ectoparasite-host associations are reported for the first time. New host and locality records presented in this study are in accordance with previous findings on the same and/or related host species in nearby South American localities.
      
      

    • Changes in Habitat Suitability for the Tick Ixodes ricinus (Acari: Ixodidae) in Europe (1900-1999). (Completo, 2006)

      ESTRADA-PEÑA, A. , VENZAL, J.M.
      EcoHealth, v.: 3 p.:154 - 162, 2006
      Palabras clave: Ixodes ricinus habitat suitability Europe 1900–1999
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      E-ISSN: 16129210
      Climatic changes may lead to drastic changes in the distribution of arthropods important in human health. We tracked changes in habitat suitability for the tick Ixodes ricinus in Europe from 1900 to 1999, using a geographically extensive gridded climate data set. For the whole period, 52% of the territory was always unsuitable for the tick. In the grid, 6.11% of the cells were classified as having a deterministic drift with positive trend and 7.4% as deterministic drift with a negative trend. A total of 17.25% of cells were classified as exhibiting a random walk behavior, with a trend to increase of habitat suitability (9.57%) or decrease (7.68%). Zones of deterministic trend extend into most of Ireland and parts of the United Kingdom and France. Total and summer rainfall primarily drive changes in habitat suitability in these sites. Areas of random walk are common in Scandinavia, central Europe, and the Balkans, with summer rainfall and temperature largely directing the changes. Sites of reported increased abundance of I. ricinus coincided with areas of increased habitat suitability over the last 20–30 years, but this feature showed a long-term random walk negative trend. Habitat suitability for I. ricinus remains relatively stable in Europe, with no sites showing permanent changes in habitat suitability (negative to sustained positive or vice versa). However, some zones in the continent showed a clear trend to increase or decrease.
      
      

    • High-resolution predictive mapping for Boophilus annulatus and B. microplus (Acari: ixodidae) in Mexico and Southern Texas. (Completo, 2006)

      ESTRADA-PEÑA, A. , VENZAL, J.M.
      Veterinary Parasitology, v.: 142 p.:350 - 358, 2006
      Palabras clave: High-resolution Satellite imagery Predictive mapping Boophilus spp. Texas
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03044017
      In this study we use high-resolution satellite imagery to map habitat suitability for both Boophilus annulatus and B. microplus over an area covering parts of Texas and northeastern Mexico. The habitat for these tick species covers widely different regions. B. microplus finds satisfactory abiotic conditions in wet coastal habitats, whereas B. annulatus finds suitable habitat in the drier inner parts of southwestern Texas and northern Mexico; the two species have very different habitat requirements.We use habitat topology and data concerning abiotic habitat suitability to understand the effects of habitat configuration on tick abundance. This is achieved using traversability (habitat permeability) and recruitment (tick abundance at the patch level) to produce a model that computes the abundance of ticks at the patch level. Results from the computation are then compared with actual data on tick outbreaks at the Texas–Mexico border, as a test of the agreement between actual tick captures and abundance model predictions.
      
      

    • Ticks (Ixodidae) on humans in South America. (Completo, 2006)

      GUGLIELMONE, A.A. , BEATI, L. , BARROS-BATTESTI, D.M. , LABRUNA, M.B. , NAVA, S. , VENZAL, J.M. , MANGOLD, A.J. , SZABÓ, M.P.J. , MARTINS, J.R. , GONZÁLEZ-ACUÑA, D. , ESTRADA-PEÑA, A.
      Experimental and Applied Acarology, v.: 40 p.:83 - 100, 2006
      Palabras clave: Ixodidae SOUTH AMERICA Ticks Humans
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      Twenty eight species of Ixodidae have been found on man in South America (21 Amblyomma, 1 Boophilus, 2 Dermacentor, 2 Haemaphysalis, 1 Ixodes and 1 Rhipicephalus species). Most of them are rarely found on man. However, three species frequently parasitize humans in restricted areas of Argentina (A. neumanni reported from 46 localities), Uruguay (A. triste from 21 sites) and Argentina–Brazil (A. parvum from 27 localities). The most widespread ticks are A. cajennense (134 localities in Argentina, Bolivia, Brazil, Colombia, Ecuador, French Guiana, Guyana, Paraguay, Suriname and Venezuela), A. ovale (37 localities in Argentina, Brazil, Ecuador, French Guiana, Guyana, Paraguay, Suriname and Venezuela) and A. oblongoguttatum (28 sites in Brazil, Colombia, French Guiana, Guyana, Suriname and Venezuela). Amblyomma aureolatum (18 localities in Argentina, Brazil, French Guiana and Paraguay), A. cajennense, and A. triste are vectors of rickettsioses to man in South America. A better understanding of the respective roles of these and other tick species in transmitting pathogens to humans will require further local investigations. Amblyomma ticks should be the main subjects of these studies followed by species of Boophilus, Dermacentor, Haemaphysalis and Rhipicephalus species. In contrast with North America, Europe and Asia, ticks of the genus Ixodes do not appear to be major players in transmitting diseases to human. Indeed, there is only one record of an Ixodes collected while feeding on man for all South America.
      
      

    • Larvae and Nymphs of Amblyomma tigrinum Koch, 1844 and Amblyomma triste Koch, 1844 (Acari: Ixodidae) Naturally Parasitizing Sigmodontinae Rodents. (Completo, 2006)

      NAVA, S. , LARESCHI, M. , ABBA, A.M. , BELDOMÉNICO, P.M. , VENZAL, J.M. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Acarologia, v.: XLVI 3-4 , p.:135 - 141, 2006
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 0044586X
      E-ISSN: 21077207
      Larvae and nymphs of Amblyomma tigrinum Koch, 1844 and Amblyomma triste Koch, 1844 were collected on Sigmodontinae rodents in Argentina. Amblyomma tigrinum was found in the phytogegraphical provinces of Chaco and Monte from the Chaco domain, while A. triste was determined in the Paranaense province of the Amazonic domain and in the Pampean province of the Chaco domain. Larvae and nymphs of A. tigrinum were found on Akodon dolores (Thomas, 1916); Akodon molinae Contreras, 1968; Akodon oenos Braun, Mares and Ojeda, 2000; Calomys musculinus (Thomas, 1913); Graomys Thomas, 1916 sp.; only larvae were found Calomys venustus (Thomas, 1894) and Phyllotis xanthopygus (Waterhouse, 1937). Larvae and nymphs of A. triste were found on Akodon azarae (Fischer, 1829); Calomys callosus Renger, 1830; Oxymycterus rufus (Fischer, 1814) and only nymphs Oligoryzomys delticola (Thomas, 1917). All these Sigmodontinae are new hosts for these tick species. Birds are considered crucial for the life cycle of A. tigrinum; however the results of this study show that Sigmodontinae rodents may also play a role for the maintenance of this tick in nature. This information also confirms the importance of Sigmodontinae as natural host for A. triste. Although on a wide basis the distribution of both tick species appears to be sympatric they colonize different ecological niches.
      
      

    • Nuevos registros de piojos Trichodectidae (Phthiraptera: Ischnocera) para Uruguay (Completo, 2006)

      VENZAL, J.M. , CASTRO, O. , DE SOUZA, C.G. , CORREA, O.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 41 163-164 , p.:31 - 34, 2006
      Palabras clave: Uruguay Phthiraptera Trichodectidae mamíferos
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03764362
      E-ISSN: 16884809
      The lice (Phthiraptera) are a poorly studied group in Uruguay. In this work new contributions for the family Trichodectidae, as well as the listing of all registered species until the moment in the country, are carried. Eutrichophilus cordiceps Mjoberg, 1910, Neotrichodectes chilensis Werneck, 1948, Stachiella fallax (Werneck, 1948) and Trichodectes galictidis Werneck, 1934 are considered new records for the country. Additionally Bovicola caprae (Gurlt, 1843) and Trichodectes canis (De Geer, 1778) are correctly documented and confirmed through material deposited in collection. In this way, the number of Trichodectidae species confirmed for Uruguay rises to 12.
      
      

    • Reptile trade and risk of exotic tick introduction into southern South American countries (Completo, 2005)

      GONZÁLEZ-ACUÑA, D. , VENZAL, J.M. , BELDOMÉNICO, P.M. , FABRY, M. , KEIRANS, J.E. , GUGLIELMONE, A.A.
      Experimental and Applied Acarology, v.: 35 p.:335 - 339, 2005
      Palabras clave: Amblyomma argentinae Amblyomma latum Python regius Reptile trade Southern South American countries Terrestrial tortoises
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      Ticks exotic for the Neotropical region were found on Python regius imported into Argentina and Chile. All ticks (7 males and 3 females) were classified as Amblyomma latum Koch, 1844 (= Aponomma latum). Additionally, four lots comprising 18 males of the Argentinean tortoise tick, Amblyomma argentinae Neumann, 1904, were found on a terrestrial tortoise, Chelonoidis chilensis, and on three terrestrial tortoises (probably C. chilensis) imported to Uruguay presumably from Argentina). These findings alert us to the risk of expanding the distribution of reptile parasites and their diseases into regions previously free of these parasites.
      
      

    • Ixodes (Ixodes) pararicinus Keirans & Clifford, 1985 (Acari: Ixodidae): Description of the Immature Stages, Distribution, Hosts, and Medical/Veterinary Importance (Completo, 2005)

      VENZAL, J.M. , ESTRADA-PEÑA, A. , BARROS-BATTESTI, D.M. , ONOFRIO, V.C. , BELDOMÉNICO, P.M.
      Systematic Parasitology, v.: 60 3 , p.:225 - 234, 2005
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01655752
      E-ISSN: 15735192
      The immature stages of the Neotropical tick Ixodes (Ixodes) pararicinus Keirans & Clifford, 1985 are described from specimens in a laboratory colony initiated from engorged females collected on cattle and larvae fed on mice and chickens. The larva and nymph of I. pararicinus are described using SEM micrographs as well as drawings for some features of the larva. Additionally, immature stages of I. pararicinus collected on wild mice and birds in Uruguay and Argentina were compared with specimens from the laboratory colony. A taxonomic key to the nymphs of the species of the ricinus complex established in the Western Hemisphere is presented. The distribution of I. pararicinus comprises Argentina, Colombia and Uruguay, but it is also probably established in Bolivia, Brazil, Chile and Peru. Most adult ticks of this species have been found on introduced domestic artiodactyles, although Neotropical deer species must have been the ancestral host. Larvae and nymphs of I. pararicinus have also been found on sigmodontine rodents and passeriform birds. Although I. pararicinus is a member of the ricinus complex, which contains the main vectors of Borrelia burgdorferi (sensu lato), there are few studies concerning its potential for pathogen transmission.
      
      

    • The Amblyomma maculatum Koch, 1844 (Acari: Ixodidae: Amblyomminae) tick group: diagnostic characters, description of the larva of A. parvitarsum Neumann, 1901, 16S rDNA sequences, distribution and hosts (Completo, 2005)Trabajo relevante

      ESTRADA-PEÑA, A. , VENZAL, J.M. , MANGOLD, A.J. , CAFRUNE, M.M. , GUGLIELMONE, A.A.
      Systematic Parasitology, v.: 60 2 , p.:99 - 112, 2005
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01655752
      E-ISSN: 15735192
      A review of the largely confused Amblyomma maculatum Koch, 1844 tick group of the subgenus Anastosiella Santos Dias, 1963 (A. neumanni Ribaga, 1902, A. maculatum, A. parvitarsum Neumann, 1901, A. tigrinum Koch, 1844 and A. triste Koch, 1844) is presented together with a discussion of the diagnostic characters used for the determination of adults, nymphs and, to a lesser extent, larvae. A key for this tick group is produced, including the description of the larva of A. parvitarsum, 1901. Sequences of 16S rDNA are obtained and compared with other Amblyomma spp., including two other species currently in Anastosiella but in the ovale tick group, A. ovale Koch, 1844 and A. aureolatum (Pallas, 1772). According to the morphology and the rDNA sequences, the maculatum group is reduced to A. maculatum (Neotropical-Nearctic), A. tigrinum (Neotropical) and A. triste (Neotropical) A. neumanni and A. parvitarsum are excluded from the subgenus. The distribution is sympatric in northern South America from where A. maculatum reaches the southern Nearctic and the range of A. tigrinum extends to the southern Neotropics. These species have been found on several domestic and wild vertebrates. A. triste and A. tigrinum have been also found on man. Their role as vectors of pathogens deserves further investigation.
      
      

    • A collection of ticks (Ixodidae) from wild birds in Uruguay. (Completo, 2005)

      VENZAL, J.M. , FÉLIX, M.L. , OLMOS, A. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Experimental and Applied Acarology, v.: 36 p.:325 - 331, 2005
      Palabras clave: Ixodidae AMBLYOMMA Ixodes Haemaphysalis Uruguay Wild birds
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      Thirty nine wild birds from seven families (Alcedinidae, Emberizidae, Furnariidae, Parulidae, Trochilidae, Turdidae and Tyrannidae) were caught from 4 to 7 of February, 2004 at Rincón da Vassoura (31º15´ S 56º03´ W) Department of Tacuarembó, Uruguay. Ninety one nymphs and forty one larvae of Ixodidae were recovered from them. The majority of ticks were conventionally identified but 16S mitochondrial rDNA sequences were obtained for larvae and nymphs of Amblyomma with uncertain specific status to be compared with sequences of species from this genus. The 16S rDNA of Haemaphysalis juxtakochi Cooley was also obtained. Most ticks, 1 nymph of Amblyomma longirostre (Koch), 5 larvae of Amblyomma aureolatum (Pallas) and 3 nymphs of Amblyomma spp., 10 nymphs and 71 larvae of H. juxtakochi, 23 nymphs and 12 larvae of Ixodes pararicinus Keirans and Clifford, were found on the 5 Turdus albicollis Vieillot and 6 Turdus rufiventris Vieillot (Turdidae) caught. One nymph of A. longirostre and 2 nymphs of I. pararicinus were collected from the only Syndactyla rufosuperciliata (Lafresnaye) (Furnariidae) captured; 1 larva of I. pararicinus was collected on one of two Basileuterus leucoblepharus (Vieillot) (Parulidae) trapped and 1 nymph of A. longirostre was found on one of nine Elaenia parvirostris Pelzeln (Tyrannidae) captured. All these findings constitute new host records with the exception of I. pararicinus on S. rufosuperciliata, and stress the importance of Turdus as hosts for I. pararicinus, H. juxtakochi and A. aureolatum. Nymphs of H. juxtakochi and Amblyomma spp. were also found on man and vegetation.
      
      

    • First record of Haemaphysalis juxtakochi Cooley, 1946 (Acari: Ixodidae) from Peru. (Completo, 2005)

      GUGLIELMONE, A.A. , ROMERO, J. , VENZAL, J.M. , NAVA, S. , MANGOLD, A.J. , VILLAVICENCIO, J.
      Systematic and Applied Acarology, v.: 10 p.:33 - 35, 2005
      Palabras clave: Ixodidae Haemaphysalis juxtakochi Lama pacos Peru
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 13621971
      E-ISSN: 20566069
      Twenty-four adult ticks were collected from South American alpacas, Lama pacos (Linnaeus, 1758), at Anexo Tambo Cañahuas (16º01’S, 71º26’W), distrito de Yanahuara, departamento Arequipa, Peru, altitude 4,300 m. Three male and 20 female ticks were identified as Amblyomma parvitarsum Neumann, 1901, a common parasite of camelids belonging to the genera Lama G. Cuvier, 1800, and Vicugna Lesson, 1842, in the Andean region of Argentina, Bolivia, Chile and Peru. The remaining tick was a female of Haemaphysalis juxtakochi Cooley, 1946. This is the first Peruvian record of H. juxtakochi, a species known from the Neotropical and southern Nearctic Zoogeographic Regions. Lama pacos is also a new host for this tick species.
      
      

    • Ixodes sigelos Keirans, Clifford & Corwin, 1976 (Acari: Ixodidae) in Argentina and southern Chile (Completo, 2005)

      GUGLIELMONE, A.A. , GONZÁLEZ-ACUÑA, D. , AUTINO, A.G. , VENZAL, J.M. , NAVA, S. , MANGOLD, A.J.
      Systematic and Applied Acarology, v.: 10 p.:37 - 40, 2005
      Palabras clave: Ixodidae Ixodes sigelos Sigmodontinae Argentina southern Chile
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 13621971
      E-ISSN: 20566069
      Single nymphs of Ixodes sigelos Keirans, Clifford & Corwin, 1976 were collected in Region XI, Chile, on Akodon olivaceus (Waterhouse 1837) in Puerto Aysen (45º23´S 72º41´W), Oligoryzomys longicaudatus (Bennett 1832) in the vicinity of Lago General Carrera (46º26´S 72º00´W) and Akodon xanthorhinus (Waterhouse 1837) in Puerto Ibañez (46º17´S 71º56´W), as well as in Tucumán Province, Argentina, on Akodon spegazzinii Thomas, 1897, 3 km SW of Hualinchay (26º20´S 65º39´W). Akodon xanthorhinus and A. spegazzinii are new hosts for I. sigelos, and these are the first records of this tick from Argentina and southern Chile. The nymph of I. sigelos and nymphs of three other Neotropical Ixodes (I. neuquenensis Ringuelet, 1947, I. nuttalli Lahille, 1913, I. stilesi Neumann, 1911) bear anterior and posterior processes on palpal article I. However, I. sigelos is unique in possessing both a basis capituli that appears winged in dorsal aspect and a bifurcate posterior process on palpal article I. These collections greatly expand the known range of I. sigelos and underscore the importance of sigmodontine rodents as hosts of Neotropical ticks, especially immature stages.
      
      

    • New hosts and localities for Ixodes auritulus Neumann, 1904 (Acari: Ixodidae) tick group, with a revision of hosts and distribution for the Neotropical Zoogeographic Region. (Completo, 2005)

      GONZÁLEZ-ACUÑA, D. , VENZAL, J.M. , KEIRANS, J.E. , ROBBINS, R.G. , IPPI, S. , GUGLIELMONE, A.A.
      Experimental and Applied Acarology, v.: 37 p.:147 - 156, 2005
      Palabras clave: Ixodidae distribution Hosts Ixodes auritulus species group Neotropical region Ticks
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      New Neotropical records are presented for ticks belonging to the Ixodes auritulus Neumann, 1904, species group, together with a review of hosts and localities from which members of this complex have previously been collected. The range of the I. auritulus species group is now understood to include Colombia, and 15 bird species are listed as new hosts. From Guatemala to southern Argentina and Chile, specimens of the I. auritulus group have been found on birds belonging to the orders Ciconiiformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Piciformes, Procellariiformes and Tinamiformes. Passeriform birds are probably the principal hosts, sustaining tick populations throughout the Neotropics. Collection data have yielded four areas – southern South America (from 56º S to 51º S), southern Brazil (25º S–22º S), south-central Peru (14º S–10º S) and Central America (10º N–15º N) – where the I. auritulus group appears to commonly parasitize birds, but additional collections may show that the range of this complex is less discontinuous than currently perceived. Several morphological differences are described for ticks within and among these areas, but it is still unclear whether the I. auritulus group comprises more than one species
      
      

    • Identificación en Uruguay de metacercaria de Ascocotyle (Phagicola) longa Digenea: Heterophyidae parasitando lisas, Mugil platanus Pisces: Mugilidae y evaluación del riesgo de zoonosis y afecciones en mascotas (Completo, 2005)

      CARNEVIA, D. , CASTRO, O. , PERRETTA, A. , VENZAL, J.M.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 40 159-160 , p.:19 - 23, 2005
      Palabras clave: Lisa Ascocotyle longa Parasitosis zoonosis
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03764362
      E-ISSN: 16884809
      The mullet (Mugil platanus) is a fish commercialized in Uruguay for human consumption and is being also studied for aquaculture. In previous works metacercariae of the family Heterophyidae were detected in a great number of fishes. The objective of the present work is to identify the species of trematode and the occurrence of infection in mullet for Uruguay. The alevins and young fishes were captured in coast of the Rio de la Plata, whereas the adults were acquired in fish market. Internal organs (heart, spleen, liver and intestine) and muscle were examined, being observed the metacercariae in fresh with the microscope. The identification of the parasite was made for experimental infection of mice and later study of trematode adult. The parasite is Ascocotyle (Phagicola) longa Ransom, 1920. The percentage of infection of alevins was 10,27 %, the one of young fishes was 100 % and the one of adults was 94,73 %. The authors wants alert that: a) this parasite affects dogs producing gastrointestinal helminthiasis; and, b) cases in human beings, that have consumed crude fish, have been diagnosed in Brazil.
      
      

    • Description of the larva and redescription of the nymph and female of Ixodes neuquenensis Ringuelet, 1847 (Acari: Ixodidae), a parasite of the endangered species Dromiciops gliroides Thomas, 1847 (Microbiotheria: Microbiotheriidae) (Completo, 2004)

      GUGLIELMONE, A.A. , VENZAL, J.M. , AMICO, G. , MANGOLD, A.J. , KEIRANS, J.E.
      Systematic Parasitology, v.: 57 p.:211 - 219, 2004
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01655752
      E-ISSN: 15735192
      The female of Ixodes neuquenensis Ringuelet, 1947 (Acari: Ixodidae) is redescribed and the nymph and larva are described from specimens collected from the endangered marsupial Dromiciops gliroides Thomas (Microbiotheria: Microbiotheriidae) in Argentina. At first sight the female of I. neuquenensis resembles a member of the subgenus Ixodes Latreille, 1795. However, the female of I. neuquenensis is peculiar in having the combination of two spurs on coxae II–IV and a pair of chitinous plaques internal to coxa I. Both the nymph and larva have an anterior and posterior process on palpal article I, characteristics of the subgenus Ixodiopsis Filippova, 1957 and some representatives of the subgenus Pholeoixodes Schulze, 1942. Analysis of 16S mitochondrial rDNA sequences showed no strong relationship with any known Ixodes subgenus.
      
      

    • First record of immature stages of Amblyomma tigrinum (Acari: Ixodidae) on wild birds in Chile (Completo, 2004)

      GONZÁLEZ-ACUÑA, D. , VENZAL, J.M. , SKEWES-RAMM, O. , RUBILAR-CONTRERAS, L. , DAUGSCHIES, A. , GUGLIELMONE, A.A.
      Experimental and Applied Acarology, v.: 33 p.:153 - 156, 2004
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 01688162
      E-ISSN: 15729702
      For the first time, larvae and nymphs of Amblyomma tigrinum ticks were found on three species of wild birds (Zenaida auriculata, Callipepla californica and Nothoprocta perdicaria) in Chile. A significant higher number of A. tigrinum was found on fledglings of Z. auriculata and N. perdicaria than on adults of these species of birds. A significant higher number was also observed on N. perdicaria living in non-irrigated areas as compared with irrigated areas. Infestation levels were 6.5, 6.3 and 10.2 ticks for Z. auriculata, C. californica and N. perdicaria, respectively. Our results suggest that birds are important in the maintenance of the life cycle of A. tigrinum ticks in the area.
      
      

    • Rickettsia parkeri in Amblyomma triste from Uruguay (Completo, 2004)Trabajo relevante

      VENZAL, J.M. , PORTILLO, A. , ESTRADA-PEÑA, A. , CASTRO, O. , CABRERA, P.A. , OTEO, J.A.
      Emerging Infectious Diseases, v.: 10 8 , p.:1493 - 1495, 2004
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 10806040
      E-ISSN: 10806059
      Our goal was to detect whether spotted fever group Rickettsia are found in the suspected vector of rickettsioses, Amblyomma triste, in Uruguay. Rickettsia parkeri was detected in A. triste, which suggests that this species could be considered a pathogenic agent responsible for human rickettsioses in Uruguay.
      
      

    • Three new species of Antricola (Acari: Argasidae) from Brazil, with a key for the known species in the genus. (Completo, 2004)

      ESTRADA-PEÑA, A. , VENZAL, J.M. , BARROS-BATTESTI, D.M. , ONOFRIO, V.C. , TRAJANO E. , LIMA FIRMINO, J.V.
      Journal of Parasitology, v.: 90 3 , p.:490 - 498, 2004
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00223395
      E-ISSN: 19372345
      Three new species of Antricola (Acari: Argasidae) are described from adult specimens collected on bat guano in different caves in Brazil. The female of Antricola guglielmonei n. sp. is easily determined by the presence of 2 smooth, depressed areas lacking setae in the posterolateral portions of the dorsum of the idiosoma, together with the partial fusion of the tubercles in the anteromedian portion of the idiosoma. The male of this species has a small spiracular plate surrounded by a pattern of tubercles disposed concentrically in its dorsal portion. Both sexes have cervical grooves very slightly marked. Antricola delacruzi n. sp. represents the only Antricola species with the dorsum of the idiosoma devoid of tubercles in both sexes and with scarce and minute setae placed over the smooth cuticle. The female of A. inexpectata n. sp. is known only from a few specimens. In this species, lines of smooth cuticle lacking setae separate the tubercles of the dorsum. In addition, there are 3 clumps of plumose setae close to the spiracular plate, in the ventrolateral portions of the idiosoma, in 3 well-delimited regions over cuticular thickenings. These 3 species share the peculiarity of a Haller’s organ with the anterior pit bearing only 7 1 2 setae. The collection of these new species in Brazilian caves greatly expands the known range of the genus. A key to the adults of all known species of the genus is provided.
      
      

    • Ornithodoros mimon Kohls, Clifford & Jones, 1969 (Acari: Argasidae) on Argentinean bats, and new records from Uruguay (Completo, 2004)

      VENZAL, J.M. , AUTINO, A.G. , NAVA, S. , GUGLIELMONE, A.A.
      Systematic and Applied Acarology, v.: 9 p.:37 - 39, 2004
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 13621971
      E-ISSN: 20566069
      A total of 18 larvae of Ornithodoros mimon Kohls, Clifford & Jones, 1969 were found on vespertilionid bats deposited in the Annexes of the Colección Mamíferos Lillo, National University of Tucumán, Argentina, or captured alive and released. Thirteen larvae were collected on Argentinean bats: Eptesicus diminutus Osgood (Salta Province), Eptesicus furinalis (d´Orbigny) (Jujuy Province) and Histioutus macrotus (Poeppig) (Tucumán Province); the remaining specimens were obtained from E. furinalis in Uruguay (Departaments of Artigas and Montevideo). These first Argentinean records expand the known range of O. mimon, as do the records from Uruguay. Eptesicus diminutus and H. macrotus are new hosts for O. mimom, which appears to be a common bat tick in the southern Neotropics, with a range that probably includes Brazil.
      
      

    • Liolaemus jamesi (Boulanger, 1891) (Reptilia: Tropiduridae), a host for the larva of Amblyomma parvitarsum Neumann, 1901 (Acari: Ixodidae). (Completo, 2004)

      GONZÁLEZ-ACUÑA, D. , VENZAL, J.M. , FABRY, M. , GUGLIELMONE, A.A.
      Systematic and Applied Acarology, v.: 9 p.:33 - 36, 2004
      Palabras clave: Ixodidae Amblyomma parvitarsum larvae Reptilia Liolaemus jamesi Andean Altiplano
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 13621971
      E-ISSN: 20566069
      Larvae of Amblyomma parvitarsum Neumann, 1901 were collected on a lizard, Liolaemus jamesi (Boulanger, 1891), captured on March 1, 2003 in the vicinity of Surire Salar (18°50´ S 69°06´ W, altitude 4250 m) within Surire National Park, which is located in the Andean Altiplano in the First Administrative Region of Chile. This is the first confirmed record of A. parvitarsum larvae on a lizard. The habitat where the infested lizard was found is shared with South American camelids, the hosts for adults of this tick species.
      
      

    • Ecological aspects of Ixodes pararicinus (Acari: Ixodidae) and other tick species parasitizing Sigmodontin mice (Rodentia: Muridae) in the Northwestern Argentina. (Completo, 2004)

      BELDOMÉNICO, P.M. , BALDI, C.J. , ORCELLET, V.M. , PERALTA, J.L. , VENZAL, J.M. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Acarologia, v.: XLIV 1-2 , p.:15 - 21, 2004
      Palabras clave: Ixodidae Ticks Argentina Muridae Sigmodontinae Ixodes pararicinus
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 0044586X
      E-ISSN: 21077207
      The present study constitutes the first contribution to the knowledge of Ixodes pararicinus dynamics on mice populations. Sigrnodontin mice were trapped for tick collection in spring 2000, summer and fa11 2001 in three environmental settings at Parque Nacional El Rey. A total of 203 mice of the genera Akodon, Calomys, Oligoryzomys, Oryzomys, and Oxynycterus (in order of abundance) were captured. Fifty-eight (58) of them were infested with 164 larvae and 62 nymphs of ixodids. The genus Calomys was significantly more infested than Akodon (P<0.0001). The predominant tick species was I. pararicinus (139 larvae and 56 nymphs). Larvae prevailed in surnmer and fall, and nymphs in spring. The leve1 of infestation was higher in adult mice, compared with juvenile and young mice (P<0.001). Other species found were Ixodes loricatus, Ixodes longiscutatus, Ixodes sp., and Amblyomma sp. The results indicate that sigmodontin mice are of irnportance for the maintenance of I. pararicinus at the study site.
      
      

    • Sigmodontinae rodents as hosts for Ixodes loricatus Neumann, 1899 (Acari: Ixodidae). (Completo, 2004)

      NAVA, S. , LARESCHI, M. , BELDOMÉNICO, P.M. , ZERPA, C. , VENZAL, J.M. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Parasite, v.: 11 4 , p.:411 - 414, 2004
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 1252607X
      E-ISSN: 17761042
      Larvae and nymphs of Ixodes loricatus Neumann, 1899 ticks (confirmed by morphological characters and by comparison of 16S mitochondrial rDNA sequences) were collected from Sigmodontinae Wagner, rodents in central and northern Argentina and Uruguay. A total of 100 larvae and 38 nymphs of I. loricatus were collected on the genera Akodon Meyen (n = 36 individuals), Calomys Waterhouse (n = 2), Oligoryzomys Bang in = 12), Oxymycterus Waterhouse (n = 9), and Scapteromys Waterhouse (n = 13). 72 larvae and 18 nymphs were collected on Akodon. Adults of I. loricatus were found in central Argentina and Uruguay on Didelphimorphia of the genera Didelphis Linnaeus and Lutreolina Thomas. Ixodes loricatus has been considered a species with strict total specificity to Didelphimorphia. Our results show that this statement may not be justified. Sigmodontinae rodents are sympatric and share habitats with the phylogenetically distant Didelphimorphia; infestation with I. loricatus seems to be its consequence. We tentatively consider I. loricatus moderately specific to Didelphimorphia.
      
      

    • Nuevos hospedadores y localidades de Ixodes sigelos e Ixodes auritulus (Acari: Ixodidae) en Chile. (Completo, 2004)

      GONZÁLEZ-ACUÑA, D. , VENZAL, J.M. , GUGLIELMONE, A.A.
      Gayana, v.: 68 1 , p.:108 - 111, 2004
      Palabras clave: Chile Ixodes Ixodes sigelos Ixodes auritulus new hosts
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 0717652X
      E-ISSN: 07176538
      An individual of Rattus norvegicus was observed infested with a nimph of Ixodes sigelos and two of Oligoryzomys longicaudatus each one infested with one larva of I. Sigelos. Both are new hosts of these ticks parasit of rodents described exclusively in Chile. In addition, the first record of the bird Cureus cureus as host of Ixodes auritulus is reported from a bird infested with a female tick. All hosts were captured in Chillán (Región VIII). The status of Ixodes species in Chile is discussed.
      
      

    • Hallazgos Bacteriológicos y Parasitológicos en una faena de ñandú (Rhea americana). (Completo, 2004)

      GIOSSA, G. , TRENCHI, H. , CASTRO RAMOS, M. , MORGADES, D. , DE SOUZA, C.G. , CASTRO, O. , CASAS, L. , SALAZAR, M. , PERDOMO, L. , VENZAL, J.M.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 39 154 , p.:11 - 16, 2004
      Palabras clave: Ñandú (Rhea americana) Salud Pública Salmonella Mycobacterium Parásitos
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias /
      Medio de divulgación: Papel
      ISSN: 03764362
      E-ISSN: 16884809
      The result of pathologic findings in 158 ñandúes slaughtered under official supervision is presented. Swabs from 40 % of the birds were obtained from intestinal tracts and gall bladders. Salmonella newport was isolated from them. Six livers were selected, three of them with granulomatous lesions. They were sent to the Tuberculosis Laboratory in the DILAVE “Miguel C. Rubino”. The samples were decontaminated and inoculated in selective media. A sample from each of the growing colonies was stained with Ziehl-Neelsen and biochemical and cultural tests were done for identification with the Runyon method. In three samples of them granulomatous lesions were present. In five of them Mycobacterium were observed through Acid-fast positive smears. Four samples were positive to Mycobacterium avium and in one of them M. intracellulare was isolated too. From 17 complete intestinal tracks sent to the Parasitology Department (FV) the following helminthes were found: Deletrocephalus dimidiatus, Monoecocestus sp. and an unidentified cestoda. From feathers a louse identified as Struthiolipeurus sp. was found . Because of the importance of the bacteriological findings for public health, special care must be taken in the future during the rearing period and during slaughter to avoid risks. Also, the recorded parasites rise the necessity of their control during rearing.
      
      

    • Cyst burden in the brains of Wistar rats fed Toxoplasma oocysts (Completo, 2003)

      FREYRE, A. , FALCÓN, D. , CORREA, O. , MÉNDEZ, J. , GONZÁLEZ, M. , VENZAL, J.M. , MORGADES, D.
      Parasitology Research, v.: 89 p.:342 - 344, 2003
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 09320113
      E-ISSN: 14321955
      Six strains of Toxoplasma oocysts were used to infect groups of 4-24 Wistar rats, with each rat being fed 101-104 oocysts from a single strain. After 2 months, the rats were killed, their brains screened for Toxoplasma cysts and then bioassayed in mice if negative. Toxoplasma was either observed in the form of brain cysts or was recovered using the bioassay, from 113 out of 138 (82%) rat brains. As few as ten oocysts were capable of initiating a brain infection that lasted for at least 2 months in eight of the nine rats inoculated. However, judging from bioassay 102-104 oocysts did not give rise to progressively higher rates of infection. Brain cysts were seen in only 68 of 138 rats (49%). The number of Toxoplasma cysts formed in the brains of rats was generally in the order of tens to hundreds. The frequency of infection in the brains with Toxoplasma and the number of brain cysts formed appeared to be influenced by the individual resistance of the rats as well as by the doses of oocysts and the Toxoplasma strains used. The information gathered is considered to be a basis for a rat model of immunity against acquired toxoplasmosis.
      
      

    • Fetal Toxoplasma infection after oocyst inoculation of pregnant rats (Completo, 2003)

      FALCÓN, D. , FREYRE, A. , MÉNDEZ, J. , GONZÁLEZ, M. , VENZAL, J.M. , MORGADES, D.
      Parasitology Research, v.: 89 p.:352 - 353, 2003
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 09320113
      E-ISSN: 14321955
      Six groups totalling 53 Wistar rats were fed 104 oocysts from one of six different Toxoplasma strains at 15 days of pregnancy. The overall transplacental transmission rate was 51%. This varied between 10% and 80%, dependent on the strain used. The strains of Toxoplasma which are more pathogenic for mice were transmitted transplacentally more frequently than the strains of intermediate or low pathogenicity. There were no statistically significant differences in the rate of congenital transmission of Toxoplasmain rats fed oocysts (present work) or cysts (previous work).
      
      

    • Amblyomma aureolatum (Pallas, 1772) and Amblyomma ovale Koch, 1844 (Acari: Ixodidae): DNA sequences, hosts and distributions. (Completo, 2003)

      GUGLIELMONE, A.A. , ESTRADA-PEÑA, A. , MANGOLD, A.J. , BARROS-BATTESTI, D.M. , LABRUNA, M.B. , MARTINS, J.R. , VENZAL, J.M. , ARZUA, M. , KEIRANS, J.E.
      Veterinary Parasitology, v.: 113 p.:273 - 288, 2003
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03044017
      DNA sequences of Amblyomma aureolatum (Pallas, 1772) and Amblyomma ovale Koch, 1844 were obtained to determine genetic differences between these tick species. Collections of these species are discussed in relation to distribution and hosts. Seven ticks collections (four from Brazil, one from Argentina, one from Uruguay and one from USA) house a total of 1272 A. aureolatum (224 males, 251 females, 223 nymphs and 574 larvae) and 1164 A. ovale (535 males, 556 females, 66 nymphs and 7 larvae). The length of the sequenced mitochondrial 16S rRNA gene fragment for A. aureolatum was 370 bp and for A. ovale was 373 bp. The DNA sequence analysis showed a 13.1% difference between the two species. Apart from one male A. ovale found on a toad, all adult ticks were found on mammals. The majority of adult specimens of both tick species were removed from Carnivora (96.1 and 84.3% of A. aureolatum and A. ovale, respectively), especially from dogs (53.1% of A. aureolatum, and 46.4% of A. ovale). Collections on wild Canidae were higher for A. aureolatum (23.3%) than for A. ovale (7.1%). On the other hand, collections of A. ovale adults on wild Felidae were higher (18.3%) than findings of A. aureolatum (9.2%). The contribution of other mammalian orders as hosts for adults of A. aureolatum and A. ovale was irrelevant, with the exception of Perissodactyla because Tapiridae contributed with 13.0% of the total number of A. ovale adults. Adults of both tick species have been found occasionally on domestic hosts (apart of the dog) and humans. Most immature stages of A. aureolatum were found on Passeriformes birds, while rodents and carnivores were the most common hosts for nymphs and larvae of A. ovale. A. aureolatum has been found estricted to the Neotropical region, covering the eastern area of South America from Uruguay to Surinam, including northeastern Argentina, eastern Paraguay, southeastern Brazil and French Guiana. A. ovale showed a distribution that covers the Neotropical region from central-northern Argentina throughout the Neotropics into the Nearctic region of Mexico with a few records from the USA, also with collection sites in Paraguay, Bolivia, most Brazilian states, Peru, Ecuador, French Guiana, Surinam, Guyana, Trinidad & Tobago, Venezuela, Colombia, Panama, Costa Rica, Nicaragua, Belize, Guatemala and several states of Mexico.
      
      

    • Primer registro de Amblyomma longirostre (Acari: Ixodidae) en Uruguay (Completo, 2003)

      VENZAL, J.M. , CASTRO, O. , CLARAMUNT, S. , GUGLIELMONE, A.A.
      Parasitología latinoamericana, v.: 58 p.:72 - 74, 2003
      Palabras clave: Uruguay Ixodidae Amblyomma longirostre birds distribution
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      E-ISSN: 07177712
      This paper describes Amblyomma longirostre, which is recorded for the first time in Uruguay. Nymphs of this species were found on a resident bird, Phylloscartes ventralis, from a locality in which a potential host to adult ticks, Sphiggurus spinosus, also inhabits. It is concluded that A. longirostre is probably resident in Uruguay, and this is the southernmost record for this species.
      
      

    • The genus Amblyomma (Acari: Ixodidae) in Chile: new records of Amblyomma argentinae (Conil, 1877) and Amblyomma tigrinum Koch, 1844. (Completo, 2003)

      GONZÁLEZ-ACUÑA, D. , VENZAL, J.M. , KEIRANS, J.E. , GUGLIELMONE, A.A.
      Systematic and Applied Acarology, v.: 8 p.:85 - 88, 2003
      Palabras clave: Ixodidae AMBLYOMMA Amblyomma argentinae Amblyomma tigrinum Chile
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 13621971
      E-ISSN: 20566069
      Two species of Amblyomma occur in Chile: A. parvitarsum and A. tigrinum. Despite published reports of A. americanum, A. maculatum and A. varium var. albida from Chile, these three taxa do not occur there. Amblyomma inflatum is described from Chile, but this species is a nomen dubium. There are Chilean records for the terrestrial tortoise tick A. argentinae, but this species is thought to be exotic because no natural populations of terrestrial tortoises exist in Chile. Herein we present new records of A. argentinae from the terrestrial tortoise Chelonoides chilensis, illegally introduced from Argentina, and of A. tigrinum from the fox Pseudalopex griseus in Chile Region V.
      
      

    • First record of Amblyomma triste Koch, 1844 (Acari: Ixodidae) and new records of Ornithodoros mimon Kohls, Clifford & Jones, 1969 (Acari: Argasidae) on Neotropical bats (Completo, 2003)

      VENZAL, J.M. , GONZÁLEZ, E.M. , CAPELLINO, D. , ESTRADA-PEÑA, A. , GUGLIELMONE, A.A.
      Systematic and Applied Acarology, v.: 8 p.:93 - 96, 2003
      Palabras clave: Ixodidae Argasidae Amblyomma triste Ornithodoros mimon Chiroptera Neotropical region
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 13621971
      E-ISSN: 20566069
      A nymph of Amblyomma triste Koch, 1844 was found on a bat Myotis albescens (E. Geoffroy 1806) (Vespertilionidae), captured in Cerro Pan de Azúcar (34º47´S 55º13´W), Departamento Maldonado, Uruguay. A retrospective search of ticks from bats deposited in the Museo Nacional de Historia Natural, Montevideo, Uruguay, yielded seven larvae of Ornithodoros mimon Kohls, Clifford & Jones, 1969, from Eptesicus furinalis (d´Orbigny 1847) (Vespertilionidae), captured in Departamento Cerro Largo; six larvae were collected at Estancia La Formosa, (31º51´S 54º12´W), north to the locality of Aceguá, and one larva was collected at Arroyo Sarandí (32º29´S 53º41´W). This is the first record of A. triste from Chiroptera, but it is doubtful that bats are important in the life cycle of this tick species. The present records of O. mimon are the first since the description of this species from larvae collected on Bolivian and Uruguayan bats more than 30 years ago.
      
      

    • Ixodid Ticks (Acari: Ixodidae) present at Parque Nacional El Rey, Argentina. (Completo, 2003)

      BELDOMÉNICO, P.M. , BALDI, C.J. , ANTONIAZZI, L.R. , ORDUNA, G.M. , MASTROPAOLO, M. , MACEDO, A.C. , RUIZ, M.F. , ORCELLET, V.M. , PERALTA, J.L. , VENZAL, J.M. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      Neotropical Entomology, v.: 32 2 , p.:273 - 277, 2003
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 1519566X
      E-ISSN: 16788052
      ; ; ; ; ; Venzal, J.M.; Mangold, A.J.; Guglielmone, A.A.
      Information on autochthonous ticks and their hosts is scarce in South America, especially in Argentina. To contribute to tick knowledge in the region, 2094 ticks were collected from vegetations, humans, and domestic and wild animals in a host-and-tick rich area of northern Argentina during six field trips conducted in 1999 (January and August), 2000 (March and November), and 2001 (March and June). The ticks were identified as Amblyomma cajennense, A. coelebs, Amblyomma sp., Haemaphysalis juxtakochi, H. leporispalustris, Ixodes pararicinus, I. loricatus, I. longiscutatum and Ixodes sp. Small mammals were mainly parasitized by immature stages of Ixodes; humans and domestic animals, predominantly by Amblyomma spp., and birds, mainly by nymphs and larvae of Haemaphysalis spp.
      
      

    • Primer registro de Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) en Rattus norvergicus (Mammalia: Rodentia) en Chile (Completo, 2003)

      GONZÁLEZ-ACUÑA, D. , VENZAL, J.M. , GUGLIELMONE, A.A.
      Gayana, v.: 67 1 , p.:121 - 122, 2003
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 0717652X
      E-ISSN: 07176538
      A male Rhipicephalus sanguineus was found on a Rattus norvegicus in the city of Chillan (Chile). This is the first record on this host in Chile. The hypothetical sanitary importance of the finding is discussed.
      
      

    • Ticks (Ixodida: Ixodidae) parasitising humans in Uruguay (Completo, 2003)

      VENZAL, J.M. , GUGLIELMONE, A.A. , ESTRADA-PEÑA, A. , CABRERA, P.A. , CASTRO, O.
      Annals of Tropical Medicine and Parasitology, v.: 97 7 , p.:769 - 772, 2003
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00034983
      E-ISSN: 13648594
      97(7): 769-772.
      During routine collections between November 1999 and November 2002, three species of tick (Amblyomma tigrinum, Amblyomma triste and Rhipicephalus sanguineus) were found on humans in southern Uruguay. The finding of R. sanguineus and A. triste on humans is of particular concern because these tick species may be involved in the transmission of the pathogens causing human rickettsioses, a type of disease already known to occur in Uruguay.
      
      

    • Argas (Persicargas) keiransi n. sp. (Acari: Argasidae) a parasite of the Chimango (Aves: Falconiformes) in Chile (Completo, 2003)

      ESTRADA-PEÑA, A. , VENZAL, J.M. , GONZÁLEZ-ACUÑA, D. , GUGLIELMONE, A.A.
      Journal of Medical Entomology, v.: 40 6 , p.:766 - 769, 2003
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00222585
      E-ISSN: 19382928
      The larva of Argas (Persicargas) keiransi Estrada-Peña, Venzal & González-Acuña n. sp. is described from specimens collected on the neck of a chimango, Milvago c. chimango (Aves: Falconiformes) in the Chillán, Chile, in the Sub-Antarctic biogeographical region. The larva of the new species shares the tarsus I setal formula with A. (P.) giganteus, these being the only two Persicargas species with three pairs of ventral setae plus both av4 and pv4 setae. However, it is unique in having a dorsal plate "V" or "U" shaped, with the anterior end open, without the typical reticulated pattern present in the remaining species of the genus.
      
      

    • Garrapatas de importancia médica y veterinaria en Uruguay (Completo, 2003)

      VENZAL, J.M. , CASTRO, O. , CABRERA, P.A. , DE SOUZA, C.G. , GUGLIELMONE, A.A.
      Entomología y Vectores, v.: 10 4 , p.:635 - 650, 2003
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      E-ISSN: 03280381
      From the 31 species quoted in the literature, 19 have been confirmed as part of the Uruguayan fauna of ticks. Two of them are of economic and public health importance in Uruguay: Boophilus microplus that infects cattle, and Amblyomma triste that infects humans. B. microplus, the cattle common tick, can cause economic losses due to direct parasitism, and can also acts as vectors of babesiosis (Babesia bovis and B. bigemina) and anaplasmosis (Anaplasma marginale). The high costs of sanitary programmes and required acaricides (to some this tick species have developed resistance) also add to the problems. A. triste, on the other hand, is the only tick involved in the transmission of Rickettsia conorii to individuals in Uruguay. As this species is particularly aggressive to humans, the necessity of epidemiological studies is stressed. Preliminary results from molecular studies on Rickettsia spp. potentially transmitted by A. triste suggest that the species implicated is not R. conorii. Other tick species that are potential vectors of human and animal pathogens in Uruguay include Ornithodoros talaje/O. puertoricensis, A. aureolatum, Ixodes pararicinus and Rhipicephalus sanguineus.
      
      

    • Garrapatas de Catamarca y Tucumán: Estudio de una colección de hospedadores silvestres y domésticos. (Completo, 2003)

      ZERPA, C. , VENZAL, J.M. , LÓPEZ, N. , MANGOLD, A.J. , GUGLIELMONE, A.A.
      FAVE, v.: 2 2 , p.:167 - 171, 2003
      Palabras clave: Ixodidae AMBLYOMMA Haemaphysalis Rhipicephalus Catamarca Tucumán
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03253112
      Durante 2003-2004 se obtuvieron garrapatas de caballos (Amblyomma neumanni) y de corzuela Mazama gouazoupira (A. neumanni y Haemaphysalis juxtakochi) en Catamarca y de perros (A. tigrinum, Rhipicephalus sanguineus), mara, Dolichotis patagonum (R. sanguineus), lampalagua, Boa constrictor (A. argentinae) y tapir, Tapirus terrestris (A. dubitatum) en Tucumán. Aunque R. sanguineus se presume que se encuentra establecida en la mayor parte de los centros urbanos argentinos, éstos son los primeros registros para Tucumán. El hallazgo sobre mara es el primero para este tipo de hospedador. También los registros de A. dubitatum y de H. juxtakochi en Tucumán y Catamarca, respectivamente, son los primeros para ambas provincias.
      
      

    • Las garrapatas de Uruguay: especies, hospedadores, distribución e importancia sanitaria. (Completo, 2003)

      VENZAL, J.M. , CASTRO, O. , CABRERA, P.A. , DE SOUZA, C.G. , GUGLIELMONE, A.A.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 38 150-151 , p.:17 - 28, 2003
      Palabras clave: Uruguay Garrapatas hospedadores distribución importancia sanitaria
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03764362
      E-ISSN: 16884809
      A list of tick species present in Uruguay is presented. The 31 species were classified as: resident (15 species), probably resident (3), accidental (1), to confirm (4), erroneous diagnosis (6) and mistaken records (2 species). The new systematic status of some species is clarified and the hosts and geographical distribution within Uruguay are indicated. Boophilus microplus is still the only species with economic significance to livestock, whereas for human health, Amblyomma triste is the most important species because of its involvement in the transmission of rickettsia. Several other species, such as Ornithodoros talaje, Ixodes pararicinus and Rhipicephalus sanguineus were identified as potential vectors of diseases to animals and humans. The introduction of transmissible microorganisms into carrier animals, ecological and climatic changes and the human colonization in certain zones of the country, are the risk factors in the transmission of tickborne diseases.
      
      

    • First records of Amblyomma auricularium (Conil, 1878) and Amblyomma pseudoconcolor Aragão, 1908 (Acari: Ixodidae) in Uruguay (Completo, 2002)

      VENZAL, J.M. , CASTRO, O. , GUGLIELMONE, A.A. , KEIRANS, J.E.
      Systematic and Applied Acarology, v.: 7 p.:109 - 111, 2002
      Palabras clave: Uruguay Ixodidae Amblyomma auricularium Amblyomma pseudoconcolor Dasypus hybridus
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 13621971
      E-ISSN: 20566069
      A. auricularium and A. pseudoconcolor are recorded for the first time in Uruguay parasitizing Dasypus hybridus. These are the first records of ixodids from dasypodids in this country.
      
      

    • Amblyomma dubitatum Neumann: description of nynph and redescription of adults, together with the description of inmatures A. triste Koch (Completo, 2002)

      ESTRADA-PEÑA, A. , VENZAL, J.M. , GUGLIELMONE, A.A.
      Acarologia, v.: 42 4 , p.:323 - 333, 2002
      Palabras clave: AMBLYOMMA SOUTH AMERICA DESCRIPTION NYMPHS NEOTROPICAL
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 0044586X
      E-ISSN: 21077207
      The adults of Amblyomma dubitatum Neumann, 1899, originally reported as "collected in southern Spain" are redescribed from the engorged female type and several flat adults collected in Uruguay and Brazil. As stated elsewhere, A. cooperi Nuttall and Warburton, 1908, a well known parasite of Hydrochaeris hydrochaeris (Linnaeus, 1766) [=Hydrochoerus hydrochaeris (L.)] in the Neotropical region, is a junior synonym of A. dubitatum. The nymph of A. dubitatum is described from semiengorged specimens collected on H. hydrochaeris. Immature stages of A. triste Koch, 1844, are described from specimens originating from engorged females and engorged larvae, respectively. A. triste is included in the maculatum group. It is easily distinguishable from other species in this group because of a unique combination of shape and size of auriculae, as well as the relative length of coxal spurs.
      
      

    • Las garrapatas del género Ixodes (Acari: Ixodidae) en Uruguay. (Completo, 2002)

      VENZAL, J.M. , CASTRO, O. , DE SOUZA, C.G.
      Veterinaria (Sociedad de Medicina Veterinaria del Uruguay), v.: 37 146 , p.:25 - 28, 2002
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 03764362
      E-ISSN: 16884809
      An update of the genus Ixodes Latreille, 1795 (Acari: Ixodidae) in Uruguay is made. Four species are presented: I. auritulus Neumann, 1904, I. longiscutatum Boero, 1944, I. loricatus Neumann, 1899 and I. pararicinus Keirans and Clifford, 1985. I. uruguayensis Kohls and Clifford, 1967 was seen to be synonymous to I. longiscutatum. Also, new hosts to the different species of the genus and notes on their distribution and biology are included.
      
      

    • Ixodes (Haemixodes) longiscutatum Boero (New Status) and I. (H.) uruguayensis Kohls & Clifford, a new synonym of I. (H.) longiscutatum (Acari: Ixodidae). (Completo, 2001)

      VENZAL, J.M. , OTEO, J.A. , GEREMIAS, V. , HERNÁNDEZ-RUSSO, Z. , GEREMIAS. V. , SANTIBÁÑEZ, S. , FACCINI, J.L.H.
      Memórias do Instituto Oswaldo Cruz, v.: 96 8 , p.:1121 - 1122, 2001
      Palabras clave: Ixodes longiscutatum Ixodes uruguayensis subgenus Haemixodes Uruguay
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      Lugar de publicación: Rio de Janeiro
      E-ISSN: 00740276
      Females of Ixodes (Haemixodes) uruguayensis Kohls & Clifford, 1967, a species whose adults were unknown until the present, were obtained in the laboratory from engorged nymphs collected on rodents (Scapteromys tumidus and Oxymycterus nasutus) in the counties of Maldonado and San José, Uruguay. Morphological characters of these females were identical to those given in the description of the female of Ixodes longiscutatum Boero, 1944. I. uruguayensis is, thus, relegated to a junior subjective synonym of I. longiscutatum. However, because of the unique morphological characters of the immature stages, the validity of the subgenus Haemixodes Kohls & Clifford, 1967 is not questioned. Therefore, the new status of Ixodes (Haemixodes) uruguayensis Kohls & Clifford, 1967 is Ixodes (Haemixodes) longiscutatum Boero, 1944.
      
      

    • Residual infection of 15 Toxoplasma strains in the brain of rats fed cysts. (Completo, 2001)

      FREYRE, A. , CORREA, O. , FALCÓN, D. , MÉNDEZ, J. , GONZÁLEZ, M. , VENZAL, J.M.
      Parasitology Research, v.: 87 p.:915 - 918, 2001
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 09320113
      E-ISSN: 14321955
      Thirty-seven groups of 4-32 Wistar rats were 2-103 cysts of 15 Toxoplasma strains. After 2 months, the rats were euthanized and their brains screened for Toxoplasma cysts and bioassayed in mice if negative. The brains of 323 of 411 rats (78.6%) were found to be infected 2 months after inoculation with Toxoplasma cysts. Two hundred cysts were necessary to infect nearly 90% of the rats. With lower doses, only 60% of the rats had residual brain infection. Brain cysts were formed only in 146 of 411 rats (35.5%). The numbers of cysts formed were in the order of tens to hundreds, only occasionally one or two thousands. The mean percentage of rats with brain cysts, and the number of cysts formed in rat brains by different inocula, increased with higher doses of cysts and then declined. This pattern is difficult to explain and similar results regarding the number of cysts formed have been published. In relation to the mean percentage of rats infected, there appears to be a plateau in infection with the higher inocula. Neither the number of rats with cysts in their brains nor the numbers of cysts formed were dependent on the Toxoplasma strain used, with the exception of one strain. Instead, individual variations were marked, and are presumably related to variations in the individual genetic resistance to Toxoplasma infection in the rat. The information gathered is considered a preliminary step for a rat model of immunity against acquired toxoplasmosis.
      
      

    • Some factors influencing transmission of Toxoplasma in pregnant rat fed cysts (Completo, 2001)

      FREYRE, A. , CORREA, O. , FALCÓN, D. , MÉNDEZ, J. , GONZÁLEZ, M. , VENZAL, J.M.
      Parasitology Research, v.: 87 p.:941 - 944, 2001
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 09320113
      E-ISSN: 14321955
      An overall 44% transplacental transmission rate was observed in 221 rats fed cysts of 12 Toxoplasma strains at 15 days of pregnancy, with a range of 0-90% transmission. Considerable variability in the transmission rate was seen among different groups of rats that received similar Toxoplasma inocula; this is attributed to genetically based susceptibility to Toxoplasma among individuals of the outbred Wistar strain of rats. Transplacental transmission was more frequent in Long Evans than in Wistar rats. Significant differences in the rate of transmission were not found between rats that were fed similar Toxoplasma inocula 6-8 days or 15 days after conception. The frequency of transmission was not affected by the strain or dose of Toxoplasma used.
      
      

  • No Arbitrados

    • Aves y garrapatas (un asunto que pica) (Completo, 2024)

      Tarragona, E.L. , Flores, F.S. , VENZAL, J.M. , Nava, S. , Lamattina, D.
      
      Aves Argentinas, v.: 71 3 , p.:26 - 33, 2024
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología /
      Medio de divulgación: Internet
      Escrito por invitación
      ISSN: 25915053
      Las garrapatas son ectoparásitos hematófagos parientes de las arañas (pertenecen al phylum Arthropoda y clase Arachnida) que parasitan a anfibios, reptiles, aves y mamíferos (vertebrados tetrápodos). Junto con los mosquitos se encuentran entre los vectores de microrganismos patógenos (ej. virus, bacterias, protozoos) de mayor relevancia médica y veterinaria. La dispersión de las garrapatas abarca todas las ecoregiones del planeta desde los alrededores de la Antártida hasta las cercanías del Ártico, pero se puede generalizar diciendo que la riqueza específica de las garrapatas es mayor en las regiones tropicales y subtropicales en relación a las templadas y frías y, si bien hay especies adaptadas a regiones desérticas, un déficit excesivo de agua no favorece la abundancia específica. En el mundo se conocen aproximadamente mil especies de garrapatas distribuidas en tres familias: Ixodidae (denominadas garrapatas duras por la presencia de una placa esclerotizada en la superficie dorsal denominada escudo y un aparato bucal que es visible dorsalmente: aproximadamente 790 especies), Argasidae (conocidas como garrapatas blandas por la ausencia de del escudo esclerotizado dorsal y un aparato bucal que no es visible dorsalmente; aproximadamente 220 especies), y Nuttalliellidae (familia monotípica ancestral representada por una única especie africana). También hay descriptos tres géneros conocidos sólo de especímenes fósiles (Compluriscutula, Cornupalpatum y Denicroton).

    • Chorlos de pastizal: Nuevas observaciones para el norte uruguayo (Completo, 2011)

      VENZAL, J.M. , STAGI, A. , MAZZULLA, J.C. , FÉLIX, M.L.
      
      Achará Digital, v.: 2 p.:10 - 13, 2011
      Palabras clave: aves de pastizal chorlos playeros nuevos registros norte de Uruguay
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Ornitología
      Medio de divulgación: Internet
      ISSN: 15103528
      E-ISSN: 16888138
      Las praderas y pastizales de Uruguay son un importante sitio de invernada para aves migratorias como chorlos y playeros, los cuales poseen diferentes grados de amenaza. Uno de los principales problemas radica en la alteración que han sufrido estos ambientes debido a la ganadería, agricultura y actualmente la forestación. Dado que la mayoría de los registros de chorlos y playeros de pastizal en Uruguay corresponden al sur y este del país, en esta nota se dan a conocer nuevos registros para el norte, entre los que se destacan los de chorlito canela (Tryngites subruficollis), batitú (Bartramia longicauda), chorlo dorado (Pluvialis dominica) y chorlo cabezón (Oreopholus ruficollis) para los departamentos de Artigas y Salto.

    • Incidencia de cercarias (Trematoda: Digenea) en una población de Drepanotrema heloicum (d´Orbigny, 1835) (Mollusca: Planorbidae) de un área suburbana del Departamento de Canelones, Uruguay (Completo, 2007)

      CASTRO, O. , DE SOUZA, C.G. , VENZAL, J.M.
      
      Comunicaciones de La Sociedad Malacológica Del Uruguay, v.: 9 90 , p.:101 - 107, 2007
      Palabras clave: Drepanotrema heloicum xifidiocercarias furcocercarias equinocercarias Pseudoquinqueserialis caviae
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 00378607
      Se caracterizó la presencia de cercarias en una población del planórbido Drepanotrema heloicum (d´Orbigny, 1835) que colonizaba una zanja y pequeños charcos adyacentes en un área suburbana del Depto. de Canelones, Uruguay. Se realizaron colectas mensuales en dicha localidad durante 15 meses (agosto 2004 a octubre 2005). De 294 caracoles examinados, 136 (46.3 %) estaban infectados, registrándose cuatro tipos distintos de cercarias: xifidiocercarias (en 58 caracoles), furcocercarias (39), oculocercarias (13) y equinocercarias (1). Además, 20 caracoles presentaron infecciones por fases de desarrollo previas a la formación de cercarias (esporocistos, rédias y formas no determinadas), y otros cinco infecciones dobles, aunque la cantidad observada de infecciones dobles fue entre tres y cuatro veces menor a la esperada. Las xifidiocercarias fueron más abundantes en verano y otoño, y las furcocercarias en primavera e invierno. La prevalencia global de infección aumentó notoriamente a partir de la categoría de talla de 7.0 - 7.9 mm, al igual que la prevalencia de las xifidiocercarias. La prevalencia de furcocercarias aumentó continuamente con la talla de los caracoles, mientras que las oculocercarias presentaron una prevalencia que decreció con la talla de los moluscos a partir de la categoría de 5.0 - 5.9 mm. Las infecciones con formas larvarias previas al estadio cercarial disminuyeron al aumentar el tamaño de los moluscos. La abundancia y diversidad de larvas de tremátodos albergadas por este caracol en un ambiente suburbano indica que parásitos de ciclos complejos pueden persistir y medrar en un hábitat fuertemente antropizado.

    • Anillamiento en El Planerón (Completo, 2005)

      LUCIENTES, J. , VENZAL, J.M.
      
      La Garcilla, v.: 124 p.:21 2005
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Ornitología
      Medio de divulgación: Papel
      ISSN: 0212923X

    • Yal Negro Prygilus fruticeti (Passeriformes, Emberizidae) en Uruguay: consideraciones sobre su estatus (Completo, 2001)

      VENZAL, J.M. , STAGI, A. , BENTOS-PEREIRA, A.
      
      Achará Digital, v.: 5 p.:6 - 7, 2001
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Ornitología
      Medio de divulgación: Papel
      ISSN: 15103528
      E-ISSN: 16888138

    • Estatus y Conservación de la Avifauna del Uruguay. (Completo, 2001)

      VENZAL, J.M. , STAGI, A.
      
      Achará Digital, v.: 5 p.:17 - 21, 2001
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Ornitología
      Medio de divulgación: Papel
      ISSN: 15103528
      E-ISSN: 16888138

    • Parásitos de aves silvestres del Uruguay. Parte I. Garrapatas. (Completo, 2000)

      VENZAL, J.M.
      
      Achará Digital, v.: 3 p.:14 - 15, 2000
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Parasitología
      Medio de divulgación: Papel
      ISSN: 15103528
      E-ISSN: 16888138

    • Nuevos registros. Aves para el Departamento de Salto (Completo, 2000)

      VENZAL, J.M. , MAZZULLA, J.
      
      Achará Digital, v.: 4 p.:14 - 16, 2000
      Areas de conocimiento:
      Ciencias Naturales y Exactas / Ciencias Biológicas / Zoología, Ornitología, Entomología, Etología / Ornitología
      Medio de divulgación: Papel
      ISSN: 15103528
      E-ISSN: 16888138

    • Presencia de Lipoptena cervi y Fasciola hepatica en Venado de Campo (O. bezoarticus). (Completo, 1994)

      HERNÁNDEZ, Z. , GUGLIELMONE, A. , PESCHERA, P. , VENZAL, J.M.
      
      Boletín Técnico de Ciencias Biológicas, v.: 4 p.:13 - 18, 1994
      Areas de conocimiento:
      Ciencias Agrícolas / Ciencias Veterinarias / Ciencias Veterinarias / Parasitología
      Medio de divulgación: Papel
      ISSN: 07975902
      A study in 3 paddocks with Ozotoceros bezoarticus [Odocoileus bezoarticus] on a farm in El Tapado, in Salto, Uruguay, revealed the presence of Lipoptena. Fasciola hepatica was also found in one of the paddocks.